• The Journal of the Korean bone and joint tumor society
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• v.15 no.1
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• pp.26-33
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• 2009

Purpose: This study was performed to investigate the maspin gene expression from osteosarcoma and to determine whether its expression correlates with clinical course of the cancer. Materials and Methods: Between 2001 and 2006, 39 patients who were diagnosed and treated surgically for osteosarcoma were included in the present study. We estimated the maspin gene expression from osteosarcoma tissue samples using RT-PCR. And we examined the correlations between the maspin expression and clinical data (post-chemotherapeutic response, local relapse or metastases). Results: Maspin was over expressed in 21 cases of 39 osteosarcoma tissues. There were significant correlations between maspin expression and the response to neoadjuvant chemotherapy, distant metastases & metastasis-free survival. In multivariate analysis, maspin low-expression was significant risk factor for distant metastases. Also, there was significant difference in metastasis-free survivals between maspin hi- expression group ($69.0{\pm}10.5%$) and low-expression group ($25.4{\pm}13.0%$). Conclusion: The degree of maspin expression in osteosarcoma was significant risk factor for distant metastases and predictive factor for metastasis-free of overall survivals. Maspin may be a useful biologic marker in evaluating the prognosis in patients with osteosarcoma and could be used as a therapeutic target clinically.

• Journal of Gastric Cancer
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• v.6 no.4
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• pp.291-294
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• 2006

F-18 FDG-PET/CT could be used to evaluate the surveillance of recurrent stomach cancer, but some cases reported as false-positives. The authors found an activated charcoal granuloma from intraperitoneal chemotherapy by using a curative resection and mitomycin C for stomach cancer. A mass behind the right colon that showed on CT 6 months after an operation in a 46-year-old male patient had no progression in size, but 36 months after the operation, an increase was seen on F-18 FDG-PET/CT, and a metastatic tumor was suspected. The tumor was resected by an explorative laparotomy and was diagnosed as being an activated charcoal granuloma based on the histologic finding. Based on this case, we should be reminded of the possibility of a false-positive on analysis of F-18 FDG-PET/CT caused by an activated charcoal granuloma in a patient who has intraperitoneal chemotherapy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.1-7
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• 1993

The 1,081 soybean genotypes including 972 black soybeans and 109 other colored soybeans were collected in 197 locations from January to April, 1991. Seed chemical components of soybean collections in relation to growth characters were evaluated to survey germplasms for black soybean breeding for seed quality. Seeds of 1,081 genotypes collected were analyzed for crude protein, crude lipid, and total sugar contents. The crude protein content was averaged to 39.8% and ranged from 34.1% to 48.0%. The average crude lipid and total sugar contents were 20.1% and 10.1%, and the ranges of those were 14.1% to 23.8% and 8.3% to 12.1%, respectively. Higher crude protein content was shown in early soybean maturity group, whereas higher total sugar content was in late group. Black soybean collections with green seed embryo tended to be higher in total sugar content than those with yellow one. Black soybean oil is generally composed of about 22% oleic, 55% linoleic, 7% linolenic acid, and 16% the others.

• Korean Chemical Engineering Research
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• v.61 no.1
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• pp.109-115
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• 2023

The extraction and adsorption characteristics for three phenolic ketones with high physicochemical similarity among phenolic compounds, which are alcohol fermentation inhibitors in lignocellulosic biomass hydrolysates, were investigated. The most suitable basic extractant for selectively separating acetosyringone from three phenol ketones by reactive extraction was found to be trioctylphosphine oxide. In addition, it was found that adsorption using XAD16, a polymer neutral resin adsorbent, or physical extraction using hexane, was a suitable separation method for separation of 4'-hydroxyacetophenone (HAP) and acetovanillone (AVO). A five-step fractionation process including extraction and adsorption mentioned above has been first proposed to separate and concentrate the three phenol ketones present at equal mass percentages. When physical extraction with n-hexane and re-extraction with an aqueous NaOH solution were used as the steps 4 and 5 in the fractionation process respectively, it was possible to obtain almost 70% or more of the purity of three phenolic ketones.

• Applied Biological Chemistry
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• v.51 no.3
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• pp.238-244
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• 2008

The essential oil was obtained from the aerial part of Caryopteris incana Miq. by steam distillation, samples were collected by headspace (HS) and solid-phase microextraction (SPME) methods, and the compositions of the essential oil were analyzed by gas chromatography-mass spectrometry (GCMS). The fragrance of the essential oil was fougere and woody. There were sixty-nine constituents in the essential oil: 28 carbohydrates, 22 alcohols, 7 acetates, 7 ketones, 3 aldehydes, and 2 others. Major constituents were 4,6,6-trimethyl [1S-($1{\alpha},2{\beta},5{\alpha}$)]-bicyclo[3.1.1]hept-3-en-2-ol (11.8%), taucadinol (9.4%), myrtenyl acetate (9.2%), pinocarvone (7.0%), 1-hydroxy-1,7-dimethyl-4-isopropyl-2,7-cyclodecadiene (6.3%), ${\delta}$-3-carene (6.2%). By SPME extraction, forty-nine constituents were identified: 22 hydrocarbons, 16 alcohols, 6 acetates, 3 ketones, and 2 ethers. Major constituents of the SPME-extracted sample were ${\delta}$-3-carene (12.6%), (-)-myrtenyl acetate (11.2%), 6,6-dimethyl-2-methylene-bicycol [3.1.1] heptan-3-o1 (10.9%), pinocarvone (9.3%). By HS extraction, ten constituents were identified: 5 hydrocarbons, 2 amines, 1 alcohol, and 2 others. Major constituents of the HS-extracted sample were (Z)-2-fluoro-2-butene (34.9%), ${\delta}$-3-carene (6.9%), 6-(4-chlorophenul)tetrahydro-2-methyl-2H-1,2-oxazine (5.9%). The $IC_{50}$ value (0.011 ${\mu}g/mg$) in MTT assay using HaCaT keratinocyte cell line was lower than those of commercially-selling rosemary and tea tree, suggesting more toxicological studies are needed for commercial use of the essential oil of Caryopteris incana Miq.

• Journal of the Korean Society of Tobacco Science
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• v.32 no.1
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• pp.41-54
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• 2010

담배 주류연은 수천가지 종류의 다양한 형태의 화학물질로 구성되어있고, 이들 화학물질의 대부분은 극히 미량의 농도로 존재하는 것으로 알려져 있다(Green and Rodgman, 1996). 담배연기 속에서 발암물질로서는 poly cyclic aromatic hydrycarbon (PAHs) 류인 B(a)P를 최초로 보고된 이후, 현재까지 69종의 화합물이 담배연기중의 발암물질로서 알려졌으며, 여기에는 9종의 PAHs 그리고 4종의 aromatic amine, nitrosoamines 및 aldehydes 그리고 다른 무기 및 유기 화합물들을 포함된다. 또한 담배 주류연에는 여러 화학물질들에 의해 유발된 생물학적 독성을 상당히 감소시키는 것으로 확인된 성분들이 존재하는 것으로 알려져 있다. 이러한 담배연기 중에 존재하는 항 발암성 효과를 나타내는 성분들의 작용 메카니즘은 발암성 물질 또는 발암성 물질의 대사산물들과 반응을 하거나, 불활성화 시키거나, 세포 구성 물질들과의 반응에서 서로 경쟁하거나, 또는 발암성의 흡수를 간섭함으로서 발암성 효과를 없애거나 또는 감소시키는 것으로 생각된다. 여러 연구자들은 담배연기 응축물에서 종양 성장의 억제 작용을 하는 성분들이 존재하는 것으로 주장 하고 있다.(Hoffmann and Griffin, 1958; Fall et al., 1964; Homburger and Tregier, 1965). 담배 연기 중에는 발암성을 나타내는 성분들과 함께 항 발암성 및 항 돌연변이성 효과를 나타내는 성분들이 존재한다는 것이 다양한 생물학적 평가 연구들에 의해 증명되었다(Slaga and DiGivanni, 1984; Fay et al., 1984; Green and Rodgman, 1996).

• Journal of the Korean Chemical Society
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• v.29 no.2
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• pp.183-190
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• 1985

The elution behaviors of the rare earths was carried out in the iminodiacetic acid solution of various pH as eluent using ion exchange resin. The optimum eluent for the quantitative separation of $Eu^{3+},\;Pr^{3+},\;Ce^{3+}\;and\;La^{3+}$ were 0.0301M IMDA solution of pH 8.00 and 9.00 at 5cm column of the resin Dowex 50${\times}$8 (100-200 mesh). The elution order of the rare earth elements was in order of the atomic number of those elements. From the elution behavior of rare earth and u.v spectrum we found that the complexed rare earth species with IMDA changed according to pH of IMDA.

• Resources Recycling
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• v.27 no.4
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• pp.44-49
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• 2018

$MoO{_4}^{2-}$ is the stable chemical species of Mo(VI) in alkaline solution. In the pH range of 2 to 6, condensation polymerization between $MoO{_4}^{2-}$ and hydrogen ion results in the formation of various polyanions of Mo(VI). Polycations of Mo(VI) begin to form when solution pH is less than 2. As the concentration of inorganic acid increases, polycations of Mo(VI) can react with the anion of the inorganic acid, resulting in the formation of heteranions of Mo(VI). The distribution of Mo(VI) species at pH < 6 depends on the concentration of Mo(V) and inorganic acid. In order to analyze the solvent extraction and ion exchange data on Mo(VI) from concentrated inorganic acid solution, it is necessary to elucidate the nature of Mo(VI) complexes.

• Journal of Sensor Science and Technology
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• v.33 no.3
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• pp.158-164
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• 2024

We developed a self-operated paper pump that can maintain a nearly constant flow rate of an aqueous solution along a paper strip channel in paper-based analytical devices (PADs). The quasi-stationary flow rate was controlled by increasing the crosssectional channel area (capillary force) using a fan-shaped absorption pad coupled with a paper strip channel. The flow rate is regulated by varying the fan angle of the circular absorbing pad. Furthermore, the flow rate can be increased by furnishing a hollow cavity at the center of a conventional paper strip channel. The rate was regulated by varying the length of the hollow paper channel in the flow rate range of 5.1-26.4 mm/min. As a preliminary work, a paper-pump-coupled PAD was fabricated, and its CV detection capability was evaluated for the redox reaction of Fe(CN)₆^+4/+3. The combination of a paper pump with a PAD resulted in an ideal CV curve with a higher limiting current and faster response time. These results are interpreted well by the Levich equation, which suggests that the paper pump is a very useful component in paper-based sensors.

• Environmental Analysis Health and Toxicology
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• v.18 no.2
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• pp.137-143
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• 2003

To validate and to estimate the chemical hazard playa very important role to environment and human health. The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this resepct, the clastogenicity of 8 synthetic chemicals was evaluated with bone marrow micronucleus assay in mice. The positive control, mitomycin C(2mg/kg,i.p.) revealed significant induction ratio of percentage of micronucleated polychromatic erythrocytes/l,000 polychromatic erythrocytes compared to carboxymethylcellulose control. The chemicals with relatively high LD$\_$50/ value such as phenylisocyanate (CAS No. 103-71-9), m-aminochlorobenzene (CAS No. 108-42-9) and 2-chloro-4-nitroaniline (CAS No. 121-87-9) revealed no significant induction of micronucleated polychromatic erythrocytes in mice. From this results, 8 synthetic chemicals widely used in industry have revealed no significant micronucleus induction of clastogenicity in mice in this experiment.