Evaluation of the Genetic Toxicity of Synthetic Chemicals (Ⅷ) - In vivo Bone Marrow Micronucleus Assay of 8 Synthetic Chemicals in Mice -

합성화학물질들의 유전독성평가(Ⅷ) -마우스의 골수세포를 이용한 8종 합성화학물질들의 생체내 소핵시험-

  • Ryu, Jae-Chun (Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Kim, Kyung-Ran (Toxicology Laboratory, Korea Institute of Science and Technology) ;
  • Kim, Youn-Jung (Toxicology Laboratory, Korea Institute of Science and Technology)
  • Published : 2003.06.01

Abstract

To validate and to estimate the chemical hazard playa very important role to environment and human health. The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this resepct, the clastogenicity of 8 synthetic chemicals was evaluated with bone marrow micronucleus assay in mice. The positive control, mitomycin C(2mg/kg,i.p.) revealed significant induction ratio of percentage of micronucleated polychromatic erythrocytes/l,000 polychromatic erythrocytes compared to carboxymethylcellulose control. The chemicals with relatively high LD$\_$50/ value such as phenylisocyanate (CAS No. 103-71-9), m-aminochlorobenzene (CAS No. 108-42-9) and 2-chloro-4-nitroaniline (CAS No. 121-87-9) revealed no significant induction of micronucleated polychromatic erythrocytes in mice. From this results, 8 synthetic chemicals widely used in industry have revealed no significant micronucleus induction of clastogenicity in mice in this experiment.

합성화학물질들이 환경중에 유입되어 인체에는 물론 환경생태계에 많은 영향을 미치고 있어 이들의 유해성 검증은 매우 중요한 일이라 할 수 있다. 실제 산업체에서 사용되는 수많은 화학물질들의 유전적 손상 유발유무는 더욱이 중요한 일이라 할 수 있다. 이에 산업체 공정과정에서 널리 사용되는 것으로 알려진 8종의 합성화학물질에 대해 마우스의 골수세포를 이용한 in vivo 소핵시험을 수행하여, 소핵형성 유발유무를 관찰하였다. 양성대조군으로 사용된 mitomycin는 음성대조군과 비교시 유의하게 소핵을 유발하는 반면, 비교적 마우스에서 높은 50% 치사량을 보이는 phenylisocyanate, m-aminochlorobenzene 및 2-chloro-4-nitroaniline 등의 합성물질들을 비롯한 나머지 5종의 물질들은 본 실험결과 통계적으로 유의하게 소핵을 유발하지 않는 것을 관찰 할 수 있었다.

Keywords

References

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