• Microbiology and Biotechnology Letters
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• v.40 no.1
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• pp.1-9
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• 2012

This study's aim was to isolate microorganisms producing agarase with a high activity, with possible applications in improving the performance of the pretreatment processes for bioethanol production. Marine algaes were collected from the south coast of Korea, from which three kinds of microorganisms were isolated. After a 4-day culture of these strains at $25^{\circ}C$, crude enzymes were obtained from culture supernatant or cell-free extract by ammonium sulfate precipitation and membrane dialysis. Agarase activity was observed in these crude enzymes. Notably higher specific activity was observed in the crude enzyme obtained from the culture supernatant rather than that from the cell-free extract. This indicates that a secreted enzyme has a much greater activity than a cellular enzyme. Crude enzymes from the GNUM08122 strain were inferred to have ${\alpha}$-agarase activity because release of p-nitrophenol was observed, possibly due to the cleavage of p-nitrophenyl-${\alpha}$-D-galactopyranoside. The 16S rRNA sequence of GNUM08122 showed a close relationship to Pseudoalteromonas issachenkonii KMM 3549 (99.8%) and Pseudoalteromonas tetraodonis IMA 14160 (99.7%), which led us to assign it to the genus Pseudoalteromonas. Biochemical and physiological study revealed that this strain can grow well at $40^{\circ}C$ under a wide range of pH (pH 4~8) in high-salt conditions (10% NaCl).

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.2
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• pp.111-118
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• 2016

Acne vulgaris is a chronic inflammatory condition characterized by comedo, papule, cyst, nodule and postinflammatory hyperpigmentation. Meanwhile, it is also induced by adverse event of drugs. Among them, acneiform folliculitis is a side effect of epidermal growth factor receptor (EGFR) inhibitor, which is an anticancer agent, and its incidence may occur in upward of 75 ~ 100% of cases. The main method of acne vulgaris treatment is oral antibiotics, retinoids, topical medication and so on. However, it is limitation that teratogenicity caused by retinoids and antibiotic resistance increased by using antibiotics. In this study, we aimed to evaluate the clinical efficacy and safety of topical recombinant human (rh) EGF in treating facial acne vulgaris. Twenty three Koreans (age: 10 ~ 29 years) with mild to moderate acne vulgar participated in the study and applied topical rhEGF cream (trouble control EGF) with 3 products (trouble control clarifying cleansing foam, trouble control all-clear filling toner, redroll calming moisture) on their face twice daily for four weeks. Several assessment methods were applied: Acne lesion counts score by investigator's global assessment, efficacy and satisfaction score by subjects. Skin sebum output level, hydration level and redness level were also measured at each visit. At the final visit, skin sebum level, transepidermal water loss, skin redness statistically decreased and acne lesions (comedone, papule) were statistically reduced. No severe side effects were observed during the study. In conclusion, topical rhEGF seems to be an effective and safe adjuvant treatment option for mild acne vulgaris.

• Journal of the Korean Society of Marine Environment & Safety
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• v.29 no.2
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• pp.87-97
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• 2023

In this study, we examined the effects of a large wildfire in the coastal area of Uljin-gun. The analysis of water quality and the dominant species, species composition, and community structure of marine algal was conducted quarterly in 2022 at Nagok (F-1), Hujeong (F-2), Bongpyeong (F-3), and Gongse Port (F-C) in Uljin-gun. As a result of water quality analysis, the pH, a factor of wildfire impact was 8.07~8.30 and 8.12~8.48 in surface and bottom layers, respectively, which are normal values in coastal waters of the East Sea, suggesting no direct impact from wildfires. By marine algal species composition, the coastal areas show the following order: Rhodophyta (58.1%) > Ochrophyta (25.8%) > Chlorophyta (14.5%) > Magnoliophyta (1.6%). By season, Undaria pinnatifida was the most dominant at Nagok and Hujeong in March and June, which in September and November, Gelidium elegans and Lithophyllum sp. were the most dominant in Bongpyeong and Gongse Port, respectively. In the cluster analysis, the stations were divided into two groups according to presence and absence of specific marine algal by season. The dominant species were U. pinnatifida, G. elegans and D. divaricata in group A, and Lithophyllum sp. was mainly present in group B. Thus, the species composition and group structure reflected the normal seasonal change pattern with water temperature variation and showed little significant difference from the control site, suggesting no direct effects of the wildfire on algae distribution in Uljin.

• Journal of Yeungnam Medical Science
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• v.4 no.2
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• pp.29-38
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• 1987

The authors studied anxiety-depression using New Anxiety-Depression Scale (NADS) in the subjects of 2.564 male and 1,252 female college freshmen of Yeungnam University. The authors collected the reports of NADS during the periods from January to February 1987, and applied NAOVA and t-test on anxiety depression scores in order to compare them between various psychosocial factors and sexes. The results are as follows : There was significant difference in the mean average of total scores between male and female students : male students scored $33.41{\pm}8.76$ and female students scored $35.34{\pm}9.02$ (P<0.001). The anxiety-depression scores relating to the items of sleep disturbance, apprehension, facial flushing and fatigue were relatively scored higher in both groups. One hundred and thirty nine male students (5.4%) showed seriously higher degree of anxiety-depression scores of 50 or more, while eighty eight female students (7.0%) showed the sane scores. There was a strong tendency toward higher anxiety-depression scores in the students who were dissatisfied with their home atmosphere, colleges, departments, familiarity of parents am those who had pessimistic views of self image in the past, presents and future in both groups (P<0.001).

• Korean Journal of Environment and Ecology
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• v.30 no.1
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• pp.98-103
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• 2016

Pyropia yezoensis were sampled from Jeonnam in Korea, Jiansu in China and Saga in Japan. Sampled P. yezoensis were analyzed proximate composition by Association official Analytical Chemists(AOAC) method, minerals by ICP-OES and amino acid by amino acid analyzer. Three areas resulted that contents of moisture and crude protein were $8.13{\pm}0.38%$ and $37.25{\pm}1.15%$, and crude lipid, ash and carbohydrate were $2.12{\pm}0.27%$, $7.41{\pm}0.36%$ and $45.06{\pm}1.30%$ respectively. In total minerals, Korea and China were $28300.19{\pm}76.39ppm$and $13945.63{\pm}7.73ppm$, and Japan was $12262.485{\pm}4.38ppm$. Amino acids, Korea and China were $265.28{\pm}0.38mg\;g^{-1}$ and $209.19{\pm}0.19mg\;g^{-1}$, and Japan was $157.61{\pm}0.43mg\;g^{-1}$. Therefore, those substance contents of P. yezoensis are significantly different between cultivation sites. The basic data may used to the environmental and nutritional study of P. yezoensis based on country sites.

• Korean Journal of Environment and Ecology
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• v.28 no.1
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• pp.10-23
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• 2014

This study was carried out to examine seaweed community structure and distribution of crustose coralline algae at the intertidal and subtidal zones of Gallam, Samchuk, eastern coast of Korea. Total 112 species including 14 of green algae, 33 of brown algae, and 65 of red algae were collected and identified. Among these species, 29 species were found throughout the year. The dominant species which contributed significantly to the total biomass were Ulva pertusa, Colpomenia spp., Sargassum fulvellum, Sargassum fusiforme, Sargassum muticum, Sargassum thunbergii, Corallina pilulifera, and Gelidium elegans. Annual seaweed biomass in dry weight was 66.7 $gm^{-2}$ and maximum biomass was recorded seasonally in summer (107.5 $gm^{-2}$), while minimum was recorded in autumn (36.2 $gm^{-2}$). In seaweed functional group analyses, coarsely branched form was the most dominant functional group constituting from 40.0 to 48.6% of the total flora. Ecological state group ESG II, as an opportunistic species, including sheet form, filamentous form, and coarsely branched form, consisted of 46~61 species, constituting 76.7~82.4%. Crustose coralline algae was observed throughout all seasons. Species of the crustose coralline algae were largely classified into Lithothamnion lemoineae, Lithophyllum okamurai, Lithophyllum yessoense, and Hydrolithon gardineri. The coverage of crustose coralline algae seems to be lower than that of other studies from 36.8% to 11% on average.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.2
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• pp.181-187
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• 2016

Algae is a potential resource with various biological activities. In this study, the anti-inflammatory effect of Grateloupia imbricata Holmes ethanol extract (GIHEE) from red algae was investigated in LPS-induced RAW 264.7 cells. As a result, reduced secretion of pro-inflammatory cytokines [tumor necrosis factors-${\alpha}$, interleukin (IL)-$1{\beta}$, and IL-6] and nitric oxide (NO) was observed in a dose-dependent manner. Expression of nuclear factor-kappaB (NF-${\kappa}B$) as well as inducible NO synthase and cyclooxygenase-2 proteins was reduced by GIHEE, suggesting that the anti-inflammatory activity of GIHEE is related to suppression of NF-${\kappa}B$ signaling pathways. In addition, GIHEE reduced phosphorylation of mitogen-activated protein kinases. These results suggest that GIHEE can be used as a potential anti-inflammatory therapeutic.

• Journal of the Korean Society of Marine Environment & Safety
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• v.15 no.3
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• pp.179-185
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• 2009

The species composition and distributional patterns in marine benthic algae at intertidal zone in Masan Bay were investigated seasonally throughout 2007. A total of 42 species, 5 Chlorophyta 8 Phaeophyta and 29 Rhodophyta were recorded, and dominant species were Enteromorpha linza, Ulva pertusa, Sargassum thunbergii, Gelidium amansii, G. divaricatum, Corallina pilulifera, Gracilaria textorii, and Polysiphonia morrowii during study periods. Especially the vertical distribution of dominant algal species was remarkable: U. pertusa and G. divaricatum were dominated in the upper part, E. intestinalis, E. linza, S. thunbergii and G. divaricatum were in the middle part, and U. pertusa, Undaria pinnatiffda, S. thunbergii, G. amansii, G. divaricatum and C. pilulifera mainly occurred in the low part of tidal zone. The numbers of species were different with seasons and stations; Higher number of species was 38 species in winter, whereas 28 species were the lowest in fall. The number of species was higher at stations 4 and 6 Mile the lower value was at stations 1 and 2 than other stations. The spatial distributional patterns of marine benthic algal composition at each station in dendrogram and MDS ordination were due to the differences of local topography and physical characteristics such as currents and water movements.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.362-372
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• 2011

The relationships among total epiphytes, algal epiphytes and eelgrass ($Zostera$ $marina$ L.) were studied at eelgrass medows from July, 1998 to July, 1999 in Yulim-ri, Yeosu, Korea. Epiphytic diatoms on eelgrass leaves were observed from July to December 1998. From the results of this study, we inferred the following three (3) conclusions : 1) As eelgrass grew older, biomass of epiphytes increased, according to relationships between the leaf length and area of eelgrass and biomass (DW, AFDW and Chl. $a$) of epiphytes. 2) According to the ratio of dry weight, ash-free dry weight and Chlorophyll of epiphytes, the algae with calcareous or siliceous skeletons, such as coralline algae or diatoms, were dominated in the epitphytic algae community. 3) The autotrophic index (AI) calculated from AFDW and Chl. $a$ of epiphytes varied from 151 to 375. However, the period of autotrophic community was shorter than heterotrophic community and the value of AI was high. From these results, we inferred that heterotrophic community, including detritus or microorganisms were dominated in the most of research period.

• The Korean Journal of Food And Nutrition
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• v.11 no.1
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• pp.99-106
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• 1998

Two kinds of carrageenan were extracted from red seaweeds, Tichocarpus crinitus, collected in The Peter the Great Bay of Russia on August, 1996. One is KC1-insoluble carrageenan and another is KC1-soluble carrageenan. The yield of KC1-insoluble carrageenan was 17.15%, which is composed of 18.06% total sulfate, 5.61% protein, 3.51% K+, 0.49% Na+, 1.66% Ca2+, 54.26% galactose, 4.68% xylose, trace of mannose and glucose. The yield of KC1-soluble carrageenan was 3.52%, which is composed of 24.06% total sulfate, 5.2% protein, 5.32% K+, 0.16% Na+, 2.80% Ca2+, 33.54% galactose, 5.48% xylose, 4.32% mannose, trace of glucose. But rhamnose was not detected in both case. FT-IR spectrum showed that the KC1-insoluble carrageenan was kappa-type carrageenan and that KC1-soluble carrageenan was lambda, iota hybrid-type carrageenan. KC1-insoluble carrageenan was very weakly formation the gel compared with KC1-insoluble carrageenan from other red seaweeds. So we investigated viscosity. Both type carrageenan was stable in the temperature until 9$0^{\circ}C$, 1 hr. The viscosity of the solution of KC1-insoluble carrageenan was increased to about two folds by K+, but was not changed by Ca2+. The viscosity of the solution of KC1-soluble carrageenan was reduced by K+ and Ca2+. Both of them was stabilized in alkali but was reduced in comparison with acid conditions. In this study, both carrageenan was expected as thickening agent than gelling agent for food additives.