• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.370-378
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• 2011

To address infra-specific relationships of Sedum kamtschaticum Fisch., and to provide the fundamental information for developing new horticultural variations, we analyzed the morphology of individuals in four natural populations (Mt. Gwangdeok, Mt. Samyeong, Mt. Yonghwa, Tongyeong) and investigated the vegetations of these area. Based on 50 morphological characters the principle component analysis (PCA) has been performed. Principle component axis 1, 2, and 3 explain 22.9%, 14.2%, and 7.4% of total variations, respectively. Dot plot of OTUs in PC2 by PC1 area showed that the areas of four populations are completely overlapped. The result of PCA and the statistics of each character indicate that all of morphological characters are overlapped in these four populations. The maximum deviations are found in the characters related in the size and shape of the leaf. In the vegetation analyses, eighteen community plots that we investigated were grouped into 10 subcommunities: subcomm. Boehmeria spicata, subcomm. Artemisia stolonifera, subcomm. Artemisia keiskeana, subcomm. Impatiens nolitangere, subcomm. Crepidiastrum chelidoniifolium, subcomm. Urtica thunbergiana, subcomm. Artemisia gmelini, subcomm. Commelina communis, subcomm. Erigeron annuus-Artemisia princeps, and typical subcommunity.

• Korean Journal of Plant Taxonomy
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• v.46 no.4
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• pp.392-404
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• 2016

The anatomical characters of the 19 taxa of Korean Juncus were reviewed in order to examine their significance in taxonomy. Cross-section analyses were conducted on the stem, leaf, and capsule. Descriptions, line drawings, tables, and a species key of Korean Juncus are provided for species identification based on anatomical characters. The results of this study showed that the arrangements of the vascular bundle, fiber, and air-space structure in the stems as well as the arrangements of the vascular bundle and parenchyma in the leaves and the development of placenta in capsules are the key characters to be used when classifying each species of Juncus. It was revealed that the studied anatomical features were more useful for identification at the subgenera and section levels than at the species level. Therefore, these anatomical characters used in conjunction with external morphological features will make a valuable contribution to establishing a natural classification system for Juncus.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.157-158
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• 2000

김(Porphyra)속 식물은 색깔, 체형, 크기, 촉감 등의 식별형질에 근거하여 분류가 시작되었고, Kurogi는 일본산 김속 식물의 분류학적 연구를 개괄하면서 김속의 식별형질로서 엽체의 세포층수, 거치상 돌기의 유무, 생식유형, 정자낭반의 형태, 정자낭 및 과포자낭 분열형식, 무성포자의 형성 유무, 지리적 분포, 각포자의 형태 및 생태적 특성 등을 종합하였다. 그러나 전통적인 분류 특징을 가지고 70종이나 되는 김을 분류한다는 것은 지금까지 불충분하여왔다. (중략)

• Animal Systematics, Evolution and Diversity
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• v.4 no.2
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• pp.103-120
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• 1988

한국에 서식하고 있는 붉은쥐속 2 종, 등줄쥐(Apodemus agrarius coreae, A. agrarius chejuensis)와 흰넓적다리 붉은쥐 (A. peninsulae peninsulae)의 염색체 및 형태적 형질의 분석을 하였다. A. agrasius coreae는 작은 형이고, A. agrarius chejuensis 와 A .peninsulae peninsulae 는 큰 형이었다. 또한A. agrarius chejuensis 는 A.peninsule peninsulae 보다도 큰 편이었다. A.peninsulae peninsulae 에 있는 B chromosomes 은 C.-negative 즉 진정염색질임이 밝혀졌다.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.95-96
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• 2003

본 연구에서는 VSV-G(vesicular stomatitis virus G glycoprotein)로 포장된 MoMLV(Moloney murine leukemia virus) retrovirus vector system을 이용하여 GFP가 발현되는 형질전환 닭을 생산하고자 하였다. GFP 유전자를 retroviral vector 내의 RSV(Rous sarcoma virus) promoter의 조절 하에 도입한 후, Gp293 세포주에서 virus 형태로 생산하였으며, 이 virus를 초원심분리로 고농축하여 stage X 계란의 배 반엽 층에 주입하여 GFP가 발현되는 형질전환 닭을 생산하였다. 생산된 닭에서의 GFP의 발현은 epifluorescence stereomicroscope를 이용하여 확인하였다. 이 방법은 기존의 여러 형질전환 가금 방법에 비하여 기술적인 용이성과 경제성을 가지므로(Muramatsu, Park and Okumura 1998), 매우 효율적이고 주목할 만한 형질전환 가금 생산 방법으로 사료된다. 형질전환 가금의 생산에서 retrovirus vector를 이용하는 방법은 다양한 종류의 표적세포에 대하여 retrovirus 고유의 감염성에 의한 외래 유전자의 전이가 용이하고, 전이된 유전자가 진정염색질 영역 내로 선택적으로 도입될 수 있으며 유전적으로 안정성을 나타내므로 매우 효과적인 방법이다. 그러나 가금에서는 초기 배 발달에 의한 급격한 세포의 수적 증가로 인해 고감염성 virus의 획득이 요구되므로, 본 연구에서는 virus의 농축에 있어 보다 안정적이고 숙주 범위에 있어서 pantropic한 VSV-G에 기반을 둔 retrovirus vector system을 확립하였다. 이 system은 기존의 형질전환 닭의 생산방법에 비해 외래 유전자의 전이에 있어서 매우 효과적인 것으로 확인되었으며, 또한 여러 유용한 생리활성물질을 분비하는 형질전환 동물의 생산에 있어서 상당한 기여를 할 것으로 사료된다.

• Korean Journal of Plant Taxonomy
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• v.43 no.3
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• pp.196-207
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• 2013

As a recent infrageneric classification of Juncus (Juncaceae), Kirschner suggested two subgenera which are distinguished by the type of inflorescence and the absence/presence of bracteole along with 10 sections which are distinguished by the characters of the leaves, the position of the inflorescence, and types of stamens and seeds. However, an intensive morphological investigation on Korean Juncus has yet to be performed. We surveyed the morphological characters of 14 taxa distributed throughout the Korean peninsula, including one North Korean taxon. The quantitative and qualitative characters of rhizomes, leaves, inflorescences, flowers, fruits, and seeds were investigated on each taxon using about 950 specimens of Korean Juncus on loan from major Korean herbaria. As a result, we provide diagnostic characters with illustrations and a key to Korean Juncus.

• KSBB Journal
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• v.7 no.3
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• pp.191-200
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• 1992

Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.

• Korean Journal of Plant Tissue Culture
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• v.27 no.3
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• pp.219-226
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• 2000

Lycopersicon peruvianum has a gametophytic self-incompatibility (GSI) mechanism controlled by a single genetic locus (S locus) with multiple alleles. S RNases, an allelic series of abundant stylar proteins, are products of the S locus in L. peruvianum and other Solanaceous plants. The $S_{11}$ RNase gene from L. peruvianum was introduced into a self-compatible (SC) species (Lycopersicon esculentum) to examine whether the expression pattern in the heterologous host mimics that in L. peruvianum. The resultant transgenic L. esculentum plants expressed the introduced gene highly in their styles, which is similar manner to the expresion in L. peruvianum. The $S_{11}$ RNase gene was expressed in the syle at a similar stage of flower development in both transgenic plants of L. esculentum and L. peruvianum without any morphological changes.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.405-431
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• 2008

Cuticle micromorphology of 22 species of Quercus and outgroup were examined by the SEM. Twenty-two species selected each two or three species in all section of the genus Quercus. The genus Trigonobalanus and Alnus are selected as outgroups. Ten characters of the inner surface and eight characters of the outer surface of the cuticle have been described. Some characters, such as the present of papillae, arrangement of subsidiary cell, shape of anticlinal cell wall are considered important character for infrageneric classification. A parsimony analysis of 18 characters resulted in 72 most parsimonious trees with its lengths of 66 steps. The topology obtained from the analysis showed two major lineages. Subgenus Cyclobalanopsis formed one clade by 75% and subgenus Quercus formed another clade by 57% bootstrap value. Based on the cuticle morphology, the two subgenus delimitation of Camus was supported. However, sect. Erythrobalanus and sect. Cerris formed one group, and sect. Lepidobalanus formed polytomy.

• Korean Journal of Ichthyology
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• v.1 no.1_2
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• pp.24-34
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• 1989

We reexamined the skulls of 14 gobionine genera of Korea to study taxonomic status of Gobioninae. The character transformation series found in the morphology of urohyal was very distinct among other skeletal characters. The gobioninae fishes of Korea were divided into 4 groups based on their morphology of urohyal as follows ; 1. Coreoleuciscus, Gnathopogon group ; The horizontal plate of urohyal has a ellipse shape with posterior edge wedged and the length is longer than that of vertical plate. 2. Hemibarbus, Squalidus group ; The horizontal plate has a elongated rhombeus shape with posterior edge pointed and the length is shorter than that of vertical plate. 3. Sarcocheilichthys, Pseudopungtungia, Pseudorasbora, Pungtungia group ; the horizontal plate has a expanded rhombeus shape, the length is longer than that of vertical plate, the hypohyal attatchment is not bifurcated, and the profile of the horizontal and vertical plate curved ventrally. 4. Ladislavia, Gobiobotia, Abbottina, Hicrophysogobio, Pseudogobio, Saurogobio group ; The horizontal plate has a pin or wedge shape expanded anteriorly, the length is shorter than that of vertical plate, and the hypohyal attatchment is thickened laterally. This results agreed with those by the adductor mandibulae complex, the geniohyoideus of cephalic muscles, and the cephalic-lateral line canals.