• Title/Summary/Keyword: 형태학적 검출

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Infection status of Tapes philippinarum collected from southern coastal areas of Korea with Parvatrema spp. (Digenea: Gymnophallidae) metacercariae (남해안 일부 지방산 반지락의 Parvatrema속 흡충 피낭유충 감염상)

  • Son, Un-Mok;Chae, Jong-Il;Lee, Sun-Hyeong
    • Parasites, Hosts and Diseases
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    • v.34 no.4
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    • pp.273-278
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    • 1996
  • An epidemiologic survey along the several sites of southern coastal areas of Korean peninsula was performed to know the infection status of Pcnlotremc app. metacercariae in Tapes phiLlppirlawm. The clams were purchased from 13 coastal areas in Kyongsangnam- do alld Chollanam-do. in September, 1990. Each of them was digested with pepsin-HCI solution and examined under a stereomicroscope for the recovery of metacercariae. A total of 232 (77.3%) out of 300 examined clams were proved to have 1 to 273 Pawatrema app. metacercariae (54.7 in average). None of the clams from Samoan-myon Kosong-gun and Dolsan-up. Yochon-gun was infected with metacercariae. However, all of the clams from Yonghyon-myon, Sachon-guu, Dohwa-myon, Kohung-gun, Ahllyang-myon. Changhung- gun and Chiryang-myon. Kangiin-gun were infected with average 71, 31, 80 and 42 metacercariae respectively. Of the clams from Kohyon-myon, Namhae-gun, Doam-myon, Kangiin-gun and Kusan-myon. Uichang-guil examined, 97.5%. 95.0% and 90.0% were infected with about 117. 76 and 28 metacercariae. In other 4 surveyed areas. Seolchon rlyoll, Namhae-gun, Hwayang-myon. Yochon-gun Byollyang-myon, Sungju-gun and Bukpyong-myon, Haenam-gun. 55.0%-80.0% of clams were positive. and their average intensity of infection ranged from 6 to 25 metacercariae. From these results, it was confirmed that Tcpes philippincrum from southern coastal areas of Korea are highly infected by Parvatrema spp. metacercariae.

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Distribution and Identification of Halophilic Bacteria in Solar Salts Produced during Entire Manufacturing Process (천일염 생산공정별 미생물 분포 조사 및 호염미생물 동정)

  • Na, Jong-Min;Kang, Min-Seung;Kim, Jin-Hyo;Jin, Yong-Xie;Je, Jeong-Hwan;Kim, Jung-Bong;Cho, Young-Sook;Kim, Jae-Hyun;Kim, So-Young
    • Microbiology and Biotechnology Letters
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    • v.39 no.2
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    • pp.133-139
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    • 2011
  • In this study, we determined the changes in microbial numbers in solar salts according to the manufacturing process and storage duration. The salt samples were harvested from salt farms in Shinan (area 2) and Yeonggwang (area 1). They were serially diluted ten-fold and then placed on 4 kinds of cultivable media (mannitol salt agar, eosin methylene blue, plate count agar, and trypticase soy agar). After incubation, we obtained 62 halophilic isolates from the salt samples. Coliform and general bacteria were not detected in all salt samples. By 16S rRNA sequencing analysis, we found 12 kinds of halophilic bacteria belonging to the genera Halobacillus, Halomonas, Bacillus, Idiomarina, Marinobacter, Pseudoalteromonas, Vibrio, Salinivibrio, Virgibacillus, Alteromonas, Staphylococcus and some un-known stains. In our study, we discovered two novel species that have a 16S rDNA sequence similarity below 97%.

The first discovery of larval Gnathostoma hispidum (Nematoda: Gnathostomidae) from a snake host, Agkistrodon breuicaudn (한국산 살모사에서 최초로 발견한 돼지악구충의 제3기 유충)

  • 손운목;이순형
    • Parasites, Hosts and Diseases
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    • v.36 no.2
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    • pp.81-90
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    • 1998
  • The present study was performed to observe the infection status of several kind of animals with indigenous Gnnthostomn in Korea, and morphological characteristics of gnathostome larvae detected from pit-viper, Askistronon breuiccudus, for the species bullfrogs. several kinds of snakes, j.e., 55 Elnphe rufodorsata, 2 Dinodon rufozonatum rufzonatum. 62 Rhnbdophis tisrinus tigrinus and 87 Askistronon app., and 438 cats were examined. A total of 21 larval gnathostomes was detected from 12 pit-vipers, A. breuicnudus. They were 2.233 x 0.343 mm in average size and covered with about 210 transverse rows of minute cuticular spines. Their characteristic head bulbs were provided with 4 rows of hooklets of which average numbers in each row were 36.8, 39.0, 41.7 and 44.3. posteriorly. In the cross sections of midgut level, the intestinal wall consisted of a single layer of 19-25 elongate epithelial cells with a single nucleus. SEM observation of the larvae revealed unique features of head bulb , cuticular spines on transverse striations and a cervical papilla. On the basis of above morphological characteristics, they were identified as the advanced third-stage larvae of Gnothostomo hispinum. It was first confirmed that the pit-viper. ABkistronon breuicaunus is the snake intermediate host of G. hispidun.

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Bacteriological Quality of Foods on Sale at Resting Places of the Highways in Korea (고속도로 휴게소에서 판매되는 식품의 세균학적 품질)

  • 서정희;이애리;김말남
    • Journal of Food Hygiene and Safety
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    • v.15 no.2
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    • pp.61-67
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    • 2000
  • Bacteriological quality evaluations were carried out for rice rolled in laver(Kimbab), Hamburger, Walnut cake and chinese noodle (Jajangmyun), which were on sale in April and May 1995, in May and July 1997 and in January 1999, at 20 different resting places on Kyung-Bu, Ho-Nam, Joong-Bu and Young-Dong Highway in Korea. Food poisoning bacteria were not detected. However numerous coliform bacteria were dectected saying that the sanitary condition of foods on sale at resting places of the highways was not hygienic. There exited 8.8$\times$10$^{4}$~6.6$\times$10$^{5}$ cells/g of coliform bacteria in all the Kimbab sold in 1995. As for Hamburger, 1.8$\times$10$^{2}$~4.7$\times$10$^{4}$ cells/g of coliform bacteria proliferated in all the samples. In 1997,E. coli was found in 16 cases and 21 cases respectively out of 22 Kimbab samples, Hamburger dealing at 7 resting places out of 14 were contaminated with 1.7$\times$10$^{2}$~1.9$\times$10$^{7}$ cells/g of coliform bacteria and Hamburgers dealing at 2 resting places were infected by 5. coli. In contrast to the Kimbab and Hamburger, all the 6 Walnut cake samples were free from the microbial pollution exhibitory that their hygienic condition was satisfactory. 3 samples out of 6 Jajangmyun were contaminated by 7.1$\times$10$^{2}$~2.0$\times$10$^{3}$ cells/g of coliform bacteria, but E. coli was not detected. Compared Kimbab sold in 1995 and 1997 with 1999, Kimbab sold in it can be said that hygienic control fur Kimbab should be performed more strictly during hot season than during cold season. Walnut cake was the safest against microbial contamination, followed by Jajangmyun, Hamburger and Kimbab in decreasing order, indicating that foods with mixed ingredients such as Kimbab and Hamburger were more susceptible to microbial infection, so that a more systematic safety control is needed for such foods during cooking, processing and distribution.

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Enhancement of Konjac Storage by Controlling pH of Coagulant and Soaking Liquid (응고제와 침지제의 pH 조절에 따른 곤약의 저장성 강화)

  • Choi, Ung-Kyu
    • Journal of Food Hygiene and Safety
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    • v.34 no.1
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    • pp.100-105
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    • 2019
  • In this study, viable cells, coliforms and food poisoning bacteria were identified according to the pH levels of the coagulant and immersion liquid during each stage in the production of konjac, and storage stability was confirmed for 3 months. A considerable number of bacteria were found in the raw material, or powdered konjac (Amorphophallus konjac), as well as in the processing water. However, it has been shown that the plastic package were safe from microorganisms. Due to the high pH of the added coagulant [2.0% $Ca(OH)_2$], no contaminating bacteria were observed after konjac jelly formation. Coliforms were not detected any of the tested steps. During the molding process, the pH of konjac was adjusted to 9.5 ~ 12.5 at intervals of 0.5, and the number of bacteria was determined. As a result, no bacteria were detected in the alkaline range above pH 11.5. The pH of the immersion liquid was adjusted to 10.0 ~ 12.5, and after hardening, the konjac were stored at room temperature for 12 weeks. As a result, no bacteria, Escherichia coli or other food poisoning bacteria were detected at pH 11.5 or higher. Based on these results, it is expected that when the pH levels of the konjac and its immersion liquid are maintained at 11.5, it should be possible to keep the product for 3 months without additional sterilization process.

Staphylococcal Scalded Skin Syndrome in Children : Comparison of the Clinical Features of That Isolated Methicillin-resistant and Methicillin-sensitive Staphylococcus aureus (포도상 구균성 열상 피부 증후군의 임상적 고찰 : Methicillin 내성과 감수성 Staphylococcus aureus 예의 비교)

  • Lee, Seong Hun;Choi, Wu Kyung;Jung, Chang Hyn;Chung, Cheol Ju;Lee, Dong Jean
    • Pediatric Infection and Vaccine
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    • v.11 no.2
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    • pp.183-191
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    • 2004
  • Purpose : The purpose of this study was to compare the clinical features of staphylococcal scalded skin syndrome(SSSS) isolated methicillin-resistant Staphylococcal aureus(MRSA) with those of SSSS isolated methicillin-sensitive Staphylococcal aureus(MSSA) in children. Methods : We retrospectively reviewed the medical records and microbiological results of 36 cases of SSSS who were admitted to the Pediatric Department of Ulsan Dong-Kang General Hospital from Jan. 1999 to Dec. 2003. Results : Among 36 cases, there were 7 generalized types, 1 abortive type in MRSA group and 5 generalized types, 6 abortive types in MSSA group. The peak incidence of age was between 2 and 5 years in both groups. SSSS were diagnosed with increasing frequency. Most of MRSA group were diagnosed in 2003(6/8). The peak seasonal incidence was fall and winter in both groups. Facial lesion was observed in all cases followed by flexural lesion in both groups. Staphylococci were isolated from the skin and throat most frequently in both groups. Upper respiratory illness and conjunctivitis were most common preceding infections in both groups. Among 8 cases of MRSA group, 4 cases were treated completely with ampicillin-sulbactam. There was no significant difference of mean duration of admission between MRSA group and MSSA group. Conclusion : There was no significant difference of clinical features between MRSA group and MSSA group although generalized types of SSSS were more common in MRSA group than in MSSA group.

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Behavior and Geochemical Characteristics of Au and Heavy Metals in the Water System at the Abandoned Bonjeong Gold Mine (본정 함금 폐광산 주변수계 내 Au와 중금속의 거동 및 지화학적 특성)

  • Cho, Kang-Hee;Kim, Bong-Ju;Oh, Su-Ji;Choi, Nag-Choul;Park, Cheon-Young
    • The Journal of Engineering Geology
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    • v.23 no.4
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    • pp.363-373
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    • 2013
  • This study investigates the dispersion and behavior of Au and heavy metals in the water system (soil, AMD and stream sediment) at the abandoned Bonjeong gold mine, based on XRD, aqua regia, sequential extraction, and physico-chemical analyses. The XRD analyses targeted quartz and kaolinite in the mine waste soil and quartz and goethite in stream sediment. The physico-chemical analyses of AMD with increasing distance from water system showed that pH increased from 3.00 to 3.19 and Eh decreased from 450 to 396 mV. The Au content in AMD ranged from 0.68 to 0.97 mg/L upstream, but was not detected downstream. The Au content of stream sediment was 13.76 to 22.85 mg/kg. Sequential extraction from stream sediment revealed 10.84% exchangeable (STEP I), 11.09% carbonates (STEP II), 25.53% Fe-Mn oxides (STEP III), 26.62% organic matter (STEP IV), and 24.61% residual (STEP V).

Effects of Cultivation Environment and Fruit Ripeness on Microbial Load in Mulberry (재배환경 및 과실 숙도가 오디의 미생물학적 부하량에 미치는 영향)

  • Ryu, Song Hee;Yun, Bohyun;Kim, Hye-Young;Choi, Ah-Hyun;Kim, Se-Ri;Kim, Won-Il;Ryu, Jae-Gee;Han, Sanghyun
    • Journal of Food Hygiene and Safety
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    • v.32 no.1
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    • pp.1-7
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    • 2017
  • This study was conducted to investigate the microbial loads in mulberry fruits depending on cultivation environment and fruit ripeness. The population levels of total aerobic bacteria in mulberry fruits collected from open field orchards were higher than those from three plots protected within plastic green houses. In regards to fruit ripeness, the levels of total aerobic bacteria in ripe black fruits were higher than those in unripe green and red mulberry. From the farms into where livestock animals were allowed to enter, Escherichia coli was detected in soil at a level of 4.26~4.94 log CFU/g and in mulberry fruits at 5.03~6.07 log CFU/g, while no coliform and E. coli were detected from where the intrusion of livestock was prevented. We also examined the density change of inoculated E. coli in mulberry fruits as they were becoming mature. While E. coli did not increase in green fruits, two and four log CFU/g increases at $20^{\circ}C$ and $37^{\circ}C$, respectively, were observed with red and fully mature black mulberries during 48 hours incubation. To ensure the food safety of mulberry, it is suggested that the introduction of E. coli into a farm through livestock should be prevented and more hygienic caution should be taken especially when the fruits are ripe.

Environmental Fate of Trichlorfon Used to Control Agelastica coerulea B. in Forest by Aerial Application (오리나무 잎벌레(Agelastica coerulea B.) 방제용 살충제 Trichlorfon(Dipterex)의 환경 동태)

  • Lee, Sung-Kyu;Kim, Yong-Hwa;Roh, Jung-Koo
    • Korean Journal of Environmental Agriculture
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    • v.5 no.2
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    • pp.119-129
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    • 1986
  • Disappearence of the trichlorfon (Dipterex) in the forest, following aerial to control Agelastica coerulea B., were studied by sampling deposits on slide glasses, soils, water, and leaves, and analysing with a gas chromatograph equipped with an electron capture detector. By analysing the amount remained on slide glasses, it was shown that the pesticide was adequately sprayed and nearly all deposit was lost in a day. The amount deposited under the tree was about 1/100 of the amount at an exposed site. Concentration of trichlorfon in creek water was 10 to 100 times as high as the acute toxic level to zooplankton for 6 to 24 hours, The rain could recontaminate the stream water up to the toxic level. Loss rate of trichlorfon from soils showed variations by sampling sites and was generally slower than from slide glasses. Amount deposited on leaves were less than the calculated or expected amount. The loss from leaves were similar to that from soil.

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Development of Agriculture Auto Hose Reel by using Wheeled Mobile Robot (바퀴구동 로봇을 이용한 농업용 자동 호스 릴 장치 개발)

  • Kim, Kyoung-Chul;Ko, Min-Hyuc;Ryuh, Beom-Sahng
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.15 no.3
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    • pp.1299-1304
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    • 2014
  • This paper is a study for developing an agriculture automatic hose reel of mobile robot. One of the important works in farming is pesticide spraying because it is related to the growth of crops. Therefore, we develop an automatic reel hose and mobile robot. Conducting kinematic analysis of steering performance, the mobile robot is designed to move smoothly even in a small space, and that is verified by simulation. To increase supplying accuracy of the automatic hose reel, the mobile robot use detecting tension mechanism on a hose and a device for the hose deployment. We conduct performance and on-farm evaluation. This system has been maximum speed of 2.5m/s, driving accuracy of ${\pm}0.18^{\circ}$ and driving safety speed of 2m/s. The system would solve an aging population and shortage of workforce in agriculture.