• KSBB Journal
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• v.6 no.1
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• pp.27-33
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• 1991

Hairy root induced by A. rhizogenes from potato tuber (Solanm tuberosum L.) synthesized the agropine and mannopine which were demonstrated with paper electrophoresis. And the starch contents in hairy root were increased gradually following the developmental stage. But protein contents were decreased. The activity of ${\beta}-glucan$ synthetase II(GSII) which is related to the cell wall biosynthesis was stimulated in hairy root on the developmental stage. And chloropromazine did not influence the activity of GS II while verapamil inhibited about 60% of the activity GS II. Therefore, these results showed $Ca^{2+}$ to be effective factor in the cell wall formation. Isozyme pattern of peroxidase was investigated in the callus and hairy root induced from potato tuber.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2006.05a
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• pp.117-121
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• 2006

본 연구는 한우의 도체 품질을 결정하는 육질 등급 판정 항목이자 경제적으로 매우 중요한 근내지방도, 배최장근 단면적 및 등지방두께에 대한 개체별 유전능력 차이를 조기에 식별하는 선발 기술을 개발하기 위해 세포내 지방산 농도 및 다양한 세포 및 지질대사를 조절하는 지 방산결합 단백질(H-FABP) 유전자의 전체 염기서열을 분석하여 SNP를 검출하고, SNP marker가 한우의 도체 및 육질 형질에 미치는 영향에 대하여 분석하고자 수행하였다. 염기 서열 분석 결과 총 6개의 SNP를 검출하였고, 이들 중 4개의 주요 SNP를 선발하여 PCR-RFLP기법으로 genotyping하고, 각 SNP marker가 한우 도체 및 육질 형질에 미치는 영향을 통계분석 하였다. H-FABP의 C3523T SNP marker가 한우의 배최장근 단면적 및 등지방 두께에 유의적인 영향을 미쳤다(p<0.05). 따라서, 본 연구를 통해 개발된 한우 H-FABP 유전자의 특정 SNP marker는 배최장근 단면적은 넓고 등지방두께는 얇은 우수한 고급육을 생산하는 한우의 조기 식별 및 육질진단에 매우 유용한 SNP분자 표지로 활용할 수 있을 것으로 기대된다.

• Korean Journal of Microbiology
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• v.38 no.2
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• pp.103-106
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• 2002

Direct PCR from intact fungal cells is not readily suitable to all fungi mainly because of difficulties in rupturing the cell walls. Microwave irradiation has been proven to be useful in fungal DNA extraction protocol. Here we describe a fast template preparation method for PCR amplification from Aspefillus nidulans conidiospores using microwave irradiation. We optimized the duration far microwave irradiation, and the amount of template DNA for PCR. Amplification from samples prepared in this manner was so efficient that we could get PCR products with size enough to identify transformants. We believe that this is a time-saving procedure for screening true transformants of A. nidulans.

• Korean Journal of Plant Taxonomy
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• v.37 no.3
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• pp.255-273
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• 2007

The morphological characters for 4 taxa of Korean Symplocaceae were examined. Based on morphological examination, numerical analysis was performed to clarify the taxonomic relationships among the taxa. It was found that epidermal characters of leaves, cellular deposition of petals, cellular boundary of petioles, growth of stigmas and pollen grains and their surfaces were useful diagonostic characters. From the results of PCA analysis, four taxa were grouped as species clusters including each populations. Four species of Symplocos were clustered as species groups with clear delimitation. Morphological, numerical, and palynological analyses supported the previous morphological studies on this family.

• The Korean Journal of Zoology
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• v.33 no.2
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• pp.241-245
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• 1990

In order to resolve the taxonomic contusion of Acheilognathus limbata from Korea, 17 morphological characters, egg form and cleavage part of A. limbata from Japan and Korea were ompared. These two groups are overlapping each other in morphometric characters mostly, but two characters of snout length and interarbital width percent in head length are distinguished between them. Moreover the Korean and the Japanese specimens are significantly different from each other based on the egg form and, cleavage part of egg. These results indicate that A. limbata from Korea and from Japan are homonyms. So we consider that the target specimens from Korea should be treated Acheifognathus sp. rather than Acheilognathus limbata until further informations are available.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.188-195
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• 2001

The anatomical characteristics of rhizome, lateral root, stem, leaf, petiole, ovary and leaf epidermis about Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz. were investigated to estimate these taxonomic values. The cross sections of stem and ovary were very similar to two species, therefore these characters were not useful for delimitation of two species, but the size and distribution numbers per 100${\mu}{\textrm}{m}$$^2$of oil cavites in rhizome, the shape of leaf and petiole in cross section, the size of leaf epidermal cell and distribution numbers per 100 ${\mu}{\textrm}{m}$$^2$of stomata were differ from two species, therefore these were thought to be useful characters for delimitation of two species.

• Animal Systematics, Evolution and Diversity
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• no.nspc2
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• pp.67-85
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• 1988

The hypotrichous ciliates (Protozoa , Ciliophora, Hypotrichida) inhabiting the Han River in Seoul were systematically investigated. Several Collections were made from 6 November 1986 to 8 November 1987 at 9 sites located in the Han River. Collected protozoans were cultured and characterized cyto-morphologically under the living and/or stained conditions, As a result, 7 species of hypotrichs in 4 families were identified of which 3 species are newly recorded from Korea ; Paraurostyla weissei (Stein, 1859_, Aspidisca cicada(O.F. Mller, 1786) and Euplotes patella (O.f.Mller, 1773). The identified species were redescribed with illustrations and photographs. The morphological similarities among them were numerically analyzed by UPGMA method, and its result of clustering concurs in general with the orthodox classification scheme.

• Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
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• v.32 no.2
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• pp.98-103
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• 2021

Plasma cell mucositis is a very rare benign disease characterized by dense lymphoplasmacytic infiltration in the submucosa layer. It appears as a reddish ulcer on the mucous membrane or as a cobblestone or nodular mass on the affected mucosa. When it involves the pharynx or larynx, the patient presents with dysphagia, voice change and dyspnea. Clinically, it is important to differentiate with malignant diseases such as extramedullary plasmacytoma, amyloidosis and sarcodosis. Several cases of mucositis in the larynx have been reported in English literature, but none have been reported in Korea. We report a case of plasma cell mucositis in the larynx with a review of literature.

• Development and Reproduction
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• v.2 no.1
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• pp.45-52
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• 1998

An enhncer detector line(EDL) having P[1ArB] insertion in X chromosome with expression of reporter gene (lacZ) in the polar cells and border cell of egg chamber was established and used to monitor the differentiation and migration of border cells during the oogenesis of Drosophila. differentiation of border cell from the anterior polar follicle cells was evident in stage-9 egg chamber of EDL149 which was characterized by migration of columnar follicle cells toward posterior of egg chamber surrounding the oocyte. Migration of border cells was observed in the stage-9 and -10 egg chambers. \beta -galactosidase activities were rapidly increased during the first 4 days after eclosion, and it coincided with the timing of border cell differentiation in the ovary during adult life. Homozygote of EDL149 showed some retardation of border cell migration , resulting absence of migration of some border cells in the anterior part of egg chamber or delayed migration of some border cells in the stage-10 egg chamber. These results suggest that the P[1ArB] of EDL149 is inserted at the locus of the structural gene required for the border cell migration. In addition to the expression in egg chambers, lacZ expression was also detected in the meiotic germ cells of testis and antenna, suggesting the possible requirement of the trapped gene function in these organ. this EDL and enhancer trapped gene might be useful for the study of developmentally regulated cell migration.

• Microbiology and Biotechnology Letters
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• v.21 no.1
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• pp.59-65
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• 1993

We isolated Actinomycetes strain GMT 1155 and purified the active compound, MT 1155, on the morphological reversion of ts/NRK cell from the isolate. MT 1155 was identified as toyocamycin having antifungal and antitumor activities from physico-chemical properties and UV, IR, $^1H$-NMR, $^13C$-NMR and mass spectrum. MT 1155 showed the morphologically reversional activity on ts/NRK cell and the cytotoxicity on CTLL cell at the final concentrations of 1.7 JlM and 0.2 11M, respectively and its $IC_{50}$ value on protein kinase A enzyme was 2.3 $\mu$M. Also it had strong antifungal activity against several pathogenic fungi but not antibacterial activity. And it did not inhibit both protein kinase C activity and the bleb-formation of K562 cell induced by phorbol esters.