• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.3
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• pp.207-214
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• 1990

Amish 집단(미국 오하이오주 홈스카운터 거주)의 vitamin E 영양상태를 24시간 회상법, 영양보충제의 사용량 조사 colorimetric법에 의한 혈장 vitamin E의 분석으로 평가하였다 평균식이 섭취 vitamin E는 7.4mg a-tocopherol equivalent였으며 평균 181.4 IU는 영양보충제로 섭취했다 전체 vitamin E의 67.7% 는 식이를 통해 섭취했으며 32.4%는 영양보충제를 복용함으로 섭취했다 평균 혈장 vitamin E 농도는 13.4mg/ml였다 혈장 vitamin E와 영양제로 복용한 vitamin E 사이에는 유의적인 상관과계가(r=0.489 p=0.0001)를 보여주었고 혈장 vitamin E와 연령과도 상관관계(r-0.216, p=0.0017)가 있었다 Amish 집단의 vitamin E 영양상태는 충분했으며 이집단 중의 어떤 자들은 고단위의 다량의 vitamin E 영양제를 복용하고 있어 vitamin E에 대한 올바른 정보를 알려주고 효율적인 영양교육을 시킬 필요성이 요청되었다.

• Analytical Science and Technology
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• v.28 no.2
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• pp.125-131
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• 2015

A method was developed and fully validated for the determination of terbutaline, a β₂-receptor agonist, in human plasma. Plasma samples were prepared by solid-phase extraction with Sep-Pak silica, followed by high-performance liquid chromatography (HPLC). The terbutaline was pre-separated from the interfering components in plasma on a Luna C18 (2) column, and terbutaline and salbutamol as an internal standard were resolved and determined on a Luna Silica column. The two columns were connected by a switching valve equipped with silica pre-column. The pre-column was used to concentrate the terbutaline in the eluent from the C18 column before back-flushing onto the silica column with fluorescence detection at an excitation/emission wavelength of 276/306 nm. The method was shown to be specific by testing six different human plasma sources. Linearity was established for a concentration range of 0.4-20.0 ng/mL with a correlation coefficient of 0.9999. The lower limit of quantitation was 0.4 ng/mL with a precision of 10.1% as C.V.%.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.1
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• pp.116-122
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• 1997

Rats were adapted to diets containing 10% cellulose,10% sodium alginate and fiber-free diet for 5 weeks. Following a 14 hour fasting, rats were fed 5g of a test meal that provided 50% energy from fat, then killed at 4 hour postprandially. Plasma and lipoprotein fraction-cholesterol levels were lower in sodium alginate-fed animals than in rats fed other diets. Plasma TG did not differ among diet treatments. Increase in TG content of HDL fraction occurred in dietary fiber groups. Intestinal apolipoprotein B level and lipase activity were lower in sodium alginate-fed group than in other dietary groups. These results suggest that chronic consumption of sodium alginate affects plasma cholesterol level as in the case of fiber supplemetation, but is less likely to modify the acute Plasma TG response to high fat meal than if a fiber supplement is incorporated into the meal.

• Korean Journal of Plant Resources
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• v.17 no.3
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• pp.323-330
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• 2004

Effects of Guemengja (Rosae Laevigatae Michx.) Extracts on serum and liver lipid composition and antioxidative capacity were investigated in rat fed high oxidized fat. Twenty eight male Sprague-Dawley rats weighing 159.35$\pm$2.17g were blocked into four groups according to body weight and raised seven weeks with basal diet (normal group, I), basal diet and 10% oxidized fat (control group, II), basal diet, 10% oxidized fat and 100mg/kgg Guemengja Extracts (100mg/kg Guemengja Extracts group, III) and basal diet, 10% oxidized fat and 200mg/kg Guemengja Extracts (200mg/kg Guemengja Extracts group, IV). The level of plasma total cholesterol and triglyceride showed a tendency to decrease, whereas the plasma HDL-cholesterol concentration revealed a tendency to increase in guemengja extracts groups. The level of liver total cholesterol showed no significantly different in all treatment groups, however the level of liver triglyceride showed a tendance to decrease in guemengja extracts groups. Thiobarbituric acid(TBARS) values in plasma and liver showed a tendence to decrease in guemengja extracts groups. The guemengja extracts samples have also decreased the plasma GOT and GPT activities, whereas they have increased the liver glutathione peroxidase, superoxide dismutase and catalase activity.

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.475-480
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• 2007

Effects of Galgeun(Pueraria radix) extracts on plasma and liver lipid composition, liver function and antioxidative capacity were investigated in rat fed high oxidized fat. Plasma total cholesterol and triglyceride concentration increased in the high oxidized fat groups, however these values showed a tendency to decrease in the Galgeun extracts groups. Plasma HDL-cholesterol concentration revealed a tendency to increase in Galgeun extracts groups. The concentration of liver total cholesterol showed no significantly different in all treatment groups, however liver triglyceride concentration showed a tendency to decrease in galgeun extracts groups. Thiobarbituric acid(TBARS) concentration in plasma and liver showed a tendency to decrease in galgeun extracts groups. The galgeun extracts samples have also decreased the plasma GOT and GPT activities, whereas they have increased the liver glutathione peroxidase, superoxide dismutase and catalase activity.

• The Korean Journal of Physiology
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• v.23 no.2
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• pp.253-261
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• 1989

Responses of atrial natriuretic peptide (ANP), aldosterone and renin release to acute volume expansion were compared in normotensive Wistar and spontaneously hypertensive rat (SHR) fed low or high-sodium diet (2 or 25 mmol Na/100 g diet). Experimental diets were fed for 6 weeks from 7-week-old and the growth rate was similar in all groups. In the morning of the experiment, catheters were inserted under ether anesthesia in femoral artery for pressure recording and blood collection, femoral vein for saline infusion, and bladder for urine collection. Then, the rats were placed in restraining cages. When the rats were recovered from anesthesia and the arterial pressure became stabilized, control urine and blood samples were collected. Then, 0.9% saline was infused for 30 min for volume expansion (3% BW). Arterial pressure was significantly higher in the high-sodium SHR but there was no difference between the two groups of Wistar rats. Control plasma levels of Na, K, ANP, renin activity, and hematocrit were not different among the 4 groups. However, plasma aldosterone level was significantly higher in the low-sodium groups. Wistar low-sodium rats showed approximately two times higher plasma aldosterone level than the SHR counterpart. Volume expansion produced a marked increase in plasma ANP level, especially in the high-sodium groups. The low-sodium groups of both strains showed approximately two-fold increase in plasma ANP level. Following a volume expansion plasma aldosterone level and renin activity decreased in all groups. There was a significant logarithmic positive correlation between plasma renin activity and aldosterone concentration. The low-sodium rats produced a greater increase in aldosterone release by small increase in plasma renin than did the high-sodium rats. The low- and high-sodium rats produced a similar degree of diuresis and natriuresis after volume expansion. However, SHR produced a greater natriuresis than did the Wistar rats. The above results indicate that regulatory mechanisms of ANP, aldosterone and renin release are different between the normotensive and hypertensive rats, and between the low- and high-sodium groups.

• Biomedical Science Letters
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• v.5 no.2
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• pp.181-190
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• 1999

To evaluate the usefulness of transforming growth factor-$\beta$1 (TGF-$\beta$1) as a new tumor marker, we determined the plasma TGF-$\beta$1 levels using sandwich ELISA assay in cancer patients. Patients with three most common adult cancers in Korea (stomach, liver and breast cancer) and children's cancers (leukemia and two kinds of solid tumor) were enrolled for the study. Furthermore, 39 individuals were subjected to age and sex-stratified plasma TGF-$\beta$1 analysis. No statistical difference was demonstrated with respect to age or sex. The mean plasma TGF-$\beta$1 level (16.0 ng/ ml) of stomach cancer patients was significantly higher than that (8.3 ng/ml) of controls. However, there was no difference among the mean plasma TGF-$\beta$1 levels of liver, breast cancer patients and controls. Seven of 16 patients (43.7%) with stomach cancer, one of 8 (12.5%) with liver cancer, and one of 7 (14.3%) with breast cancer showed higher TGF-$\beta$1 levels compared to controls. Plasma TGF-$\beta$1 concentrations of five leukemic children remained in the normal range regardless of the remission state. In contrast, initial high TGF-$\beta$1 levels from two children with solid tumors returned to normal range on surgical resection of tumors. From the above results, we could conclude that plasma TGF-$\beta$1 levels of apparently healthy individuals seem to be rather constant irrespective of difference in age or sex, and the plasma TGF-$\beta$1 has the limited value as a screening test for the diagnosis of aforementioned adult cancers because of its low sensitivity. Finally, additional studies need to be pursed for the large number of stomach cancer and pediatric solid tumor patients in order to reach a secure conclusion on the usefulness of plasma TGF-$\beta$1 as a tumor marker in these patients.

• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.2
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• pp.308-313
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• 1997

To investigate the effects of Angelica keiskei Koidz on cholesterol metabolism, male Sprague Dawley rats were fed diets containing 5% of A. keiskei flour for 6 weeks. A. keiskei decreased plasma contents of total cholesterol, LDL-cholesterol and triglyceride while showing no change in HDL-cholesterol, resulting in reduction of atherosclerotic index. Decrease in liver concentration of triglyceride and increases in fecal excretion of cholesterol, total neutral steroid and bile acid were also observed. Those results suggest that A. keiskei improves hypercholesterolemia through, at least in part, reducing the absorption of cholesterol and enhancing the catabolism of cholesterol to bile acids.

• Journal of fish pathology
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• v.20 no.2
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• pp.139-145
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• 2007

To decrease stress in eel (Anguilla japonica) during its culture or transportation, aspirin (ASA) known as analgesic, antiinflammatory and antithrombic agent was administrated by dipping or oral routes. Concentrations of aspirin (ASA) and salicylic acid (SA) in eel plasma were simultaneously measured by a high performance liquid chromatography (HPLC). The plasma was acidified with 0.2 M HCl and 0.2 M orthophosphoric acid, and mixed with acetonitrile. ASA and SA extracted with acetonitrile were analyzed by the HPLC equipped with reversed phase Novapak C18 column (4 ㎛ silica, 150×4 mm) and UV detector(237 nm). The mobile phase consisted of 740 ㎖ water, 900 ㎕ orthophosphoric acid (85%) and 180 ㎖ acetonitrile. The retention times of ASA, SA and 2-methylbenzoic acid(MBA) were 4.8 min, 8.4 min and 11.5 min, respectively. The limit of quantification was 0.01 ㎍/㎖ for SA and 0.05 ㎍/㎖ for ASA. The mean recovery from eel plasma was 70.8～99.6% for ASA and 95.2～100.3% for SA. This HPLC method was applied to analyze ASA and SA of eel plasma after either dipping in a concentration of 20 ppm or feeding the feed supplemented with 50 ㎎/kg BW. Only SA was detected in eel plasma after the administration of ASA by dipping or oral routes because the drug was quickly decomposed into SA in eel plasma. The amount of SA in eel plasma reached the highest value at 3hr in dipping and 7 days in oral administration. When the ASA-administrated eel were kept in ASA free aquaria, 0.02-0.03 ㎍/㎖ of SA were detected 48 hr after the administration in both routes.

• The Journal of Korean Orthopaedic Ultrasound Society
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• v.4 no.2
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• pp.111-122
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• 2011

Platelet rich plasma (PRP) has been widely used nowadays for several common orthopaedic-related sports medicine conditions with the theoretical basis that PRP supplies numerous autologous growth factors from concentrated platelets needed to promote the healing process of injured tissue. Even though there are many basic sciences, animal studies and some clinical studies regarding PRP injections for musculoskeletal injuries which suggested good results, it is difficult to compare these various studies due to marked methodological differences such as PRP preparation method, the timing, volume and number of injection, and the outcome measurement tool. In addition, many studies have no control groups or a limited sample size, and there are few prospective randomized controlled trials assessing the efficacy of PRP injections. Therefore, well designed high-quality randomized studies are required to confirm the preliminary results until now and provide scientific evidence to support its use, and the paucity of scientific clinical evidence suggest that the administration of PRP on humans for musculoskeletal injuries should be performed with caution.