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MicroRNA Profile in the Helicobacter pylori-infected Gastric Epithelial Cells (Helicobacter pylori 감염 위상피세포에서 MicroRNA 발현 변화)

  • Chang Whan Kim;Sung Soo Kim;Tae Ho Kim;Woo Chul Chung;Jae Kwang Kim
    • Journal of Digestive Cancer Research
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    • v.5 no.2
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    • pp.105-112
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    • 2017
  • Background: The expression of miRNAs in response to Helicobacter pylori infection has not been well explored. The aims of this study were to evaluate the H. pylori associated miRNAs in the gastric epithelial cells. Methods: We investigated gastric epithelial cell-line (HS3C) exposed H. pylori over 3 months and AGS cell-line (AGS) exposed H. pylori for 6 hour. After the extraction of miRNA from these cell-lines, microarray and real time PCR were performed to confirm the alteration of expression. Results: All 12 miRNAs chosen for real-time PCR are based on the result of microarray and their potential functions related to H. pylori infection. miR-21, miR-221, miR-222 were upregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-99b, miR-200b, miR-203b and miR-373 were downregulated in the H. pylori infected AGS cell for 6 hours and HS3C cells. miR-23a, miR-23b, miR-125b, miR-141 and miR-155 were upregulated in HS3C cell line but not in H. pylori infected AGS cell for 6 hours. Conclusion: miR-21, miR-99b, miR-125b, miR-200b, miR-203b, miR-221, miR-222, and miR-373 are supposed to be related with oncogenesis of H. pylori infection. Further studies are needed for the evaluation of the function of these confirmed miRNAs.

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The Relationship between Korean college students' Self-Construal and Alexithymia: Mediation Effects of Ambivalence over Emotional Expressiveness and Emotion Suppression according to the Deficiency of Independent Self-Construal (한국 대학생의 자기관과 감정표현불능증의 관계: 상호독립적 자기관 결핍에 따른 정서표현양가성 및 정서억제의 매개 효과)

  • Soyoung Kwon;Jarang Kwak;Bia Kim;Donghoon Lee
    • Korean Journal of Culture and Social Issue
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    • v.25 no.2
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    • pp.101-118
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    • 2019
  • Alexithymia refers to a psychological deficit of identifying and describing one's feelings. It has benn reported that the level of alexithymia of East-Asians is generally higher than that of Western Europeans. Recently one research conducted in U.S. suggested that the interdependent self-construal from East-Asians' collectivism culture might be a cause of this cross-cultural difference. In the current study, we examined the relationship between the level of independent and interdependent self-construal of Korean college students and their level of alexithymia, as well as their ambivalence over emotional expressiveness and emotion suppression. The correlation analysis shows that the Korean students' alexithymia level does not correlate with their level of interdependent self-construal, but negatively correlates with their level of independent self-construal. Moreover, it is also correlated with the level of ambivalence over emotional expressiveness(AEE) and emotion suppression(ES). Thus, we setup a double-mediation model between the deficiency of independent self-construal and alexithymia via AEE and ES, and estimate mediation effects using Hayes and Preacher(2014)s' Process analysis. The results show that the deficiency of independent self-construal has a direct effect as well as indirect effects of AEE and ES on the alexithymia level. Further analysis on the indirect effects reveals that the mediation effect of AEE and the double mediation effect of ES via AEE are significant, but the mediation effect of ES is not significant. Current results imply that the interdependent self-construal from the traditional collectivistic culture may not cause Korean college students' problems on the emotional expression, but the relatively lower independent self-construal may cause them. The deficiency of independent self-constural may raise up the level of self-defensive ambivalence over emotional expressiveness and suppress emotional expression by themselves, which can result in alexithymia.

Predicting the Potential Habitat and Future Distribution of Brachydiplax chalybea flavovittata Ris, 1911 (Odonata: Libellulidae) (기후변화에 따른 남색이마잠자리 잠재적 서식지 및 미래 분포예측)

  • Soon Jik Kwon;Yung Chul Jun;Hyeok Yeong Kwon;In Chul Hwang;Chang Su Lee;Tae Geun Kim
    • Journal of Wetlands Research
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    • v.25 no.4
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    • pp.335-344
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    • 2023
  • Brachydiplax chalybea flavovittata, a climate-sensitive biological indicator species, was first observed and recorded at Jeju Island in Korea in 2010. Overwintering was recently confirmed in the Yeongsan River area. This study was aimed to predict the potential distribution patterns for the larvae of B. chalybea flavovittata and to understand its ecological characteristics as well as changes of population under global climate change circumstances. Data was collected both from the Global Biodiversity Information Facility (GBIF) and by field surveys from May 2019 to May 2023. We used for the distribution model among downloaded 19 variables from the WorldClim database. MaxEnt model was adopted for the prediction of potential and future distribution for B. chalybea flavovittata. Larval distribution ranged within a region delimited by northern latitude from Jeju-si, Jeju Special Self-Governing Province (33.318096°) to Yeoju-si, Gyeonggi-do (37.366734°) and eastern longitude from Jindo-gun, Jeollanam-do (126.054925°) to Yangsan-si, Gyeongsangnam-do (129.016472°). M type (permanent rivers, streams and creeks) wetlands were the most common habitat based on the Ramsar's wetland classification system, followed by Tp type (permanent freshwater marshes and pools) (45.8%) and F type (estuarine waters) (4.2%). MaxEnt model presented that potential distribution with high inhabiting probability included Ulsan and Daegu Metropolitan City in addition to the currently discovered habitats. Applying to the future scenarios by Intergovernmental Panel on Climate Change (IPCC), it was predicted that the possible distribution area would expand in the 2050s and 2090s, covering the southern and western coastal regions, the southern Daegu metropolitan area and the eastern coastal regions in the near future. This study suggests that B. chalybea flavovittata can be used as an effective indicator species for climate changes with a monitoring of their distribution ranges. Our findings will also help to provide basic information on the conservation and management of co-existing native species.

Conceptual Model of Establishing Lifestyle (Lifestyle-DEPER [Decision, Execution, Personal Factor, Environment, Resources]) and Lifestyle Intervention Strategies (라이프스타일 형성 모델(Lifestyle-DEPER [Decision, Execution, Personal Factor, Environment, Resources])과 건강을 위한 라이프스타일 중재 전략)

  • Park, Ji-Hyuk;Park, Hae Yean;Hong, Ickpyo;Han, Dae-Sung;Lim, Young-Myoung;Kim, Ah-Ram;Nam, Sanghun;Park, Kang-Hyun;Lim, Seungju;Bae, Suyeong;Jin, Yeonju
    • Therapeutic Science for Rehabilitation
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    • v.12 no.4
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    • pp.9-22
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    • 2023
  • The Lifestyle-DEPER (Decision, Execution, Personal Factors, Environment, Resources) model explains lifestyle formation. Lifestyles are shaped through the decision, execution, and habituation stages. Factors influencing the establishment of a lifestyle are categorized as environmental, resource, and personal. The environment encompasses our surroundings and social, physical, cultural, and virtual environments. Resources refer to what individuals possess, such as health, time, economic, and social resources. Personal factors include competencies, needs, and values. At the lifestyle establishment stage, each of these factors influences a different stage. These collective processes are referred to as events, encompassing both personal and social events. Health-related lifestyle factors include physical activity, nutrition, social relationships, and occupational participation. These are the goals of lifestyle intervention. The intervention strategy based on the Lifestyle-DEPER model, called KEEP (Knowledge, Evaluation, Experience, Plan), is a comprehensive approach to promoting a healthy lifestyle by considering lifestyle formation stages and their influencing factors. This study introduces the Lifestyle-DEPER model and presents a lifestyle intervention strategy (KEEP) to promote health. Further research is required to validate the practicality of the model after applying interventions based on the lifestyle construction model.

Selection of Forage Corn Varieties Adapted to High Latitude (The South of Mt. Suyang) (고위도 기후대 재배 적합 국산 사료용 옥수수 품종 선발)

  • Jae-Han Son;Hwan-Hee Bae;Young Sam Go;Jun-Young Ha;Bonil Ku;Man-Kee Baek;Jeong-Ju Kim;Beom-Young Son;Tae-Wook Jung
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.43 no.4
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    • pp.216-224
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    • 2023
  • Since maize (Zea mays L.) originated in central and south America, it requires warm climate conditions throughout its growing season. Growth halts when night-time temperatures drop below 10℃, and the plant may die if temperature reach -1.7℃. Thus, temperature should be maintained between 10 and 30℃ from seeding to maturity. The germination temperature for maize should be at least 8-11℃, whit an optimal range 32-34℃. Since temperature significantly affects the germination rate and period, it plays a crucial role in maize growth. In this study, we evaluated the quantity and feed value of 11 major varieties to determine those best suited for maize cultivation as feed in higher latitude, specifically in Democratic People's of Republic of Korea, below 38 degrees north. A cultivation test was also conducted in Suwon in Republic of Korea, to assess adaptability in areas south of Mt. Suyang. Among the varieties tested, Shinhwangok2 reached silking the fastest, in 65 days, while Gwangpyeongok took the longest at 75 days. The stem length of all varieties exceeded 230 cm. Gwangpyeongok had the tallest stems, while Daanok and Shinhwangok2ho displayed the highest ear ratios. Dacheongok presented the highest values in both dry matter and TDN quantity, with 31,420 kg/ha and 21,66 kg/ha respectively. Pyeonggangok had the highest crude protein content at 8.0%. TDN (%) ranged from 57-68%, with Hwangdaok reaching up to 68%. Based on these findings, Dacheongok and Pyeonggangok appear to be the most suitable varieties for cultivation in terms of both quantity and feed value.

Comparative Study on the Carbon Stock Changes Measurement Methodologies of Perennial Woody Crops-focusing on Overseas Cases (다년생 목본작물의 탄소축적 변화량 산정방법론 비교 연구-해외사례를 중심으로)

  • Hae-In Lee;Yong-Ju Lee;Kyeong-Hak Lee;Chang-Bae Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.258-266
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    • 2023
  • This study analyzed methodologies for estimating carbon stocks of perennial woody crops and the research cases in overseas countries. As a result, we found that Australia, Bulgaria, Canada, and Japan are using the stock-difference method, while Austria, Denmark, and Germany are estimating the change in the carbon stock based on the gain-loss method. In some overseas countries, the researches were conducted on estimating the carbon stock change using image data as tier 3 phase beyond the research developing country-specific factors as tier 2 phase. In South Korea, convergence studies as the third stage were conducted in forestry field, but advanced research in the agricultural field is at the beginning stage. Based on these results, we suggest directions for the following four future researches: 1) securing national-specific factors related to emissions and removals in the agricultural field through the development of allometric equation and carbon conversion factors for perennial woody crops to improve the completeness of emission and removals statistics, 2) implementing policy studies on the cultivation area calculation refinement with fruit tree-biomass-based maturity, 3) developing a more advanced estimation technique for perennial woody crops in the agricultural sector using allometric equation and remote sensing techniques based on the agricultural and forestry satellite scheduled to be launched in 2025, and to establish a matrix and monitoring system for perennial woody crop cultivation areas in the agricultural sector, Lastly, 4) estimating soil carbon stocks change, which is currently estimated by treating all agricultural areas as one, by sub-land classification to implement a dynamic carbon cycle model. This study suggests a detailed guideline and advanced methods of carbon stock change calculation for perennial woody crops, which supports 2050 Carbon Neutral Strategy of Ministry of Agriculture, Food, and Rural Affairs and activate related research in agricultural sector.

Associations Between Heart Rate Variability and Symptom Severity in Patients With Somatic Symptom Disorder (신체 증상 장애 환자의 심박변이도와 증상 심각도의 연관성)

  • Eunhwan Kim;Hesun Kim;Jinsil Ham;Joonbeom Kim;Jooyoung Oh
    • Korean Journal of Psychosomatic Medicine
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    • v.31 no.2
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    • pp.108-117
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    • 2023
  • Objectives : Somatic symptom disorder (SSD) is characterized by the manifestation of a variety of physical symptoms, but little is known about differences in autonomic nervous system activity according to symptom severity, especially within patient groups. In this study, we examined differences in heart rate variability (HRV) across symptom severity in a group of SSD patients to analyze a representative marker of autonomic nervous system changes by symptoms severity. Methods : Medical records were retrospectively reviewed for patients who were diagnosed with SSD based on DSM-5 from September 18, 2020 to October 29, 2021. We applied inverse probability of treatment weighting (IPTW) methods to generate more homogeneous comparisons in HRV parameters by correcting for selection biases due to sociodemographic and clinical characteristic differences between groups. Results : There were statistically significant correlations between the somatic symptom severity and LF (nu), HF (nu), LF/HF, as well as SD1/SD2 and Alpha1/Alpha2. After IPTW estimation, the mild to moderate group was corrected to 27 (53.0%) and the severe group to 24 (47.0%), and homogeneity was achieved as the differences in demographic and clinical characteristics were not significant. The analysis of inverse probability weighted regression adjustment model showed that the severe group was associated with significantly lower RMSSD (β=-0.70, p=0.003) and pNN20 (β=-1.04, p=0.019) in the time domain and higher LF (nu) (β=0.29, p<0.001), lower HF (nu) (β=-0.29, p<0.001), higher LF/HF (β=1.41, p=0.001), and in the nonlinear domain, significant differences were tested for SampEn15 (β=-0.35, p=0.014), SD1/SD2 (β=-0.68, p<0.001), and Alpha1/Alpha2 (ß=0.43, p=0.001). Conclusions : These results suggest that differences in HRV parameters by SSD severity were showed in the time, frequency and nonlinear domains, specific parameters demonstrating significantly higher sympathetic nerve activity and reduced ability of the parasympathetic nervous system in SSD patients with severe symptoms.

Is 'Life Satisfaction' satisfactory?: Complementing the measurement of subjective well-being ('삶의 만족'은 만족스러운가: 주관적 웰빙 측정의 보완)

  • Jung-Ho Kim;Jhe Min You;Kyung Hyun Suh;Seong Kyeon Lim;Sun-Joo Kim;Mirihae Kim;Suja Gong;Tae-Young Kan;Jee-Sun Lee;Jungeun Hwang
    • Korean Journal of Culture and Social Issue
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    • v.15 no.1
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    • pp.187-205
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    • 2009
  • Based on the Motivational States Theory(MOST), the present research expanded and complemented Kim(2007)'s proposal to add the Life Satisfaction Expectancy Scale(LSES) to Diener et al's Satisfaction With Life Scale(SWLS) to measure subjective well-being(SWB). In the present study, the Life Satisfaction Motivation Scale(LSMS) was introduced to measure the strength of motivation for life satisfaction in general. Two hundred and eighty six college students participated in this study. Factor analyses revealed a two-factor structure, with the factors corresponding to life satisfaction and life satisfaction expectancy. Measures of internal and temporal reliability show the LSMS to be a good complement for the measure of SWB(The LSMS showed high internal and test-retest reliability). It was found that the addition of the LSES provided a significant increment in predictive power over the SWLS in the prediction of various factors related with well-being prediction. Exceptionally, in the prediction of anger the LSMS had the most predictive power. There were some differences between male and female students in the correlations among life satisfaction, life satisfaction expectancy and life satisfaction motivation and well-being-related factors. The merits of including LSES in the measurement of subjective well-being and the limitations of this study are discussed.

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The effects of skill-utilization based underemployment on self-esteem and mental health: Employment commitment and social support as moderators (기술활용도 측면의 불완전고용이 재취업자의 자아존중감과 정신건강에 미치는 영향: 일에 대한 몰입과 사회적 지지의 조절효과를 중심으로)

  • Youn-Hee Roh ;Myung Un Kim
    • Korean Journal of Culture and Social Issue
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    • v.16 no.3
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    • pp.355-383
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    • 2010
  • In this study, the effects of skill-utilization based underemployment on self-esteem and mental health were examined. Also the moderating effects of employment commitment and social support were verified. Data obtained by a longitudinally designed survey with the reemployed (N=153) after job loss were used. The skill-utilization based underemployment was measured in two ways. One is measured in the way that use objective data, the ratio of education - 'educational requirement for the current job' divided by 'individual educational attainment.' The other is measured in the way that use psychological and self-reported measure, two factors of overqualification - the absence of opportunity for growth (no-growth) and the occupational mismatch in educational attainment, skills and experiences (mismatch). The main effect revealed that the no-growth made self-esteem lower and mental health (GHQ-social maladjustment) worse. The significant interaction of the ratio of education and employment commitment indicates that the negative effects of underemployment measured by the ratio of education on mental health (GHQ-social maladjustment, GHQ-depression/anxiety) were greater for those perceiving low employment commitment than for those perceiving high employment commitment. The significant interaction of the ratio of education and social support indicates that the negative effects of underemployment measured by the ratio of education on mental health (GHQ-depression/anxiety) were greater for those perceiving low social support than for those perceiving high social support. The two moderators also interacted with the two factors of perceived overqualification. Employment commitment had significant interaction effects with the no-growth and the mismatch on self-esteem and mental health (GHQ-depression/anxiety), whereas social support had a significant interaction only with the no-growth on mental health (GHQ-depression/anxiety). The significant interactions show that employment commitment and social support function as buffers in reducing the negative effects of skill-utilization based underemployment on self-esteem and mental health.

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Remodeling and Damage of the Garden According to the Park Project in Deoksugung Palace During the Japanese Colonial Period (일제강점기 덕수궁(德壽宮) 공원화에 따른 정원의 개조와 훼손)

  • OH Junyoung
    • Korean Journal of Heritage: History & Science
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    • v.56 no.3
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    • pp.234-252
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    • 2023
  • This study looked at the modification of major gardens while making Deoksugung Palace (德壽宮) a park in the Japanese colonial era. This is because landscaping work was carried out in various places from 1932 to 1933 to open Deoksugung Palace, which used to be an imperial palace, as a public recreation space. In particular, major gardens such as the front yard of Seokjojeon Hall (石造殿), the back yard of Hamnyeongjeon Hall (咸寧殿), and the back yard of Jeukjodang Hall (卽阼堂) were greatly transformed into different shapes from the original. During the first phase of construction in 1932, a water tank was installed in Seokjojeon Hall Garden, creating the first water space. This water tank was originally a structure installed in the front yard of Injeongjeon Hall (仁政殿) of Changdeokgung Palace (昌德宮). Around 1909, a water tank installed in the front yard of Injeongjeon Hall was relocated to Seokjojeon Garden in the process of turning Deoksugung Palace into a park. The water tank moved from the front yard of Injeongjeon Hall was a factor that transformed the central area of Seokjojeon Garden into a water space, and a fountain installed to replace the water tank remains to this day. The backyard of Hamnyeongjeon Hall was also renovated into a new shape during the first phase of construction. Originally, there was a terraced flowerbed called Hwagye (花階) in the backyard of Hamyujae Hall (咸有齋) and Hamnyeongjeon Hall, and it was restored from the construction that took place after the Great Fire of Deoksugung Palace. In the process of turning Deoksugung Palace into a park, a three-stage stonework was built in the front yard of Jeonggwanheon Pavilion (靜觀軒) which renovated the Hwagye in the backyard of Hamyujae Hall and Hamnyeongjeon Halll. The stonework built at that time was used as a peony garden to provide visitors with attractions after the opening of Deoksugung Palace, and it remains today with the name Jeonggwanheon's Hwagye. The backyard of the Jeukjodang Hall area is a case of damage in the second phase of construction in 1933. Like the backyard of Hamnyeongjeon Hall, the backyard of Jeukjodang Hall, where the Hwagye was originally built, was converted into a Japanese-style garden in the process of turning Deoksugung Palace into a park. The site where the Hwagye was demolished was decorated with a Japanese-style garden centered on mounding, small roads, and landscaping stones, as well as topographic control and planting work. Although there have been minor changes since liberation, the backyard of the Jeukjodang Hall area is still based on a Japanese-style garden created by turning Deoksugung Palace into a park.