• Journal of Plant Biotechnology
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• v.44 no.1
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• pp.82-88
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• 2017

Transgenic lily plants have been obtained after particle bombardment, using PDS-1000/He system and scale explants of lilies, followed by PPT (D-L-phosphinothricin) selection. In this study, scales of the lily plants cv. 'red flame' were bombarded with a plasmid containing the bar gene as a selectable marker, and the AtSIZ gene as a gene of interest, showing salt tolerance and drought tolerance respectively, and both being driven by the CaMV 35S promoter. For optimization of a protocol, factors which optimized and showed a high transformation efficiency under following conditions, were considered: a bombardment pressure of 1100 psi, a target distance of 6 cm and $1.0{\mu}m$ of gold particle, and 24-h pre-culture and post-culture on MS medium containing 0.2 M sorbitol and 0.2 M mannitol as osmoticum agents. After bombardment, all the bombarded scales of lily were transferred to MS medium without selective agents, for a week. Subsequently, these bombarded scales were transferred to a selection MS medium containing 10 mg/l PPT, and incubated for a month for further selection, after which they were cultured for another 4-8 weeks with a 4-week subculture regime on the same selection medium. After transferring into hormone-free MS medium, the PPT-resistant scales with shoots were successfully rooted and regenerated into plantlets. PCR analysis revealed that the surviving putatively transformed plantlets indicated the presence of both the bar gene and the AtSIZ gene. In conclusion, when 100 scales of lily cv. Red flame are bombarded, this study produced approximately 17-18 transgenic plantlets with an optimized bombardment protocol. The protocol described here can contribute to the breeding program of lilies.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.22 no.4
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• pp.183-189
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• 2012

Surface treatments and their effects on high temperature properties for the Hastelloy X, which is a promising candidate alloy for high temperature heat-transport system, have been evaluated. For TiAlN and $Al_2O_3$ overlay coatings, the two different PVD (physical vapor deposition) methods using an arc discharge and a sputtering, were applied, respectively. In addition, a different surface treatment method of the diffusion coating by a pack cementation of Al (aluminiding) was also adopted in this study. To achieve enhanced thermal oxidation resistance at $1000^{\circ}C$ by suppressing the inhomogeneous formation of thick $Cr_2O_3$ crust at the surface region, a study for the surface modification methods on the morphological and structural properties of Hastelloy X substrates has been conducted. The structural and compositional properties of each sample were characterized before and after heat-treatment at $1000^{\circ}C$ under air and He environment. The results showed that the Al diffusion coating showed the more enhanced high temperature properties than the overlay coatings such as the suppressed thick $Cr_2O_3$ crust formation and lower wear loss.

• Journal of Radiation Protection and Research
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• v.33 no.3
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• pp.113-120
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• 2008

In this study, The TRITGO code was introduced, which can predict the amount of tritium production, it's transport, removal, distribution and the level of contamination for the produced hydrogen by the tritium on the VHTR (very high temperature gas cooled reactor). The TRITGO code was improved so that the permeation to the IS Iodine Sulfide) loop for producing the hydrogen can be simulated. The contamination level of the produced hydrogen by the tritium was predicted by the improved code for the VHTR with 600MW thermal power. The contamination level for the produced hydrogen by tritium was predicted as 0.055 Bq/$H_2-g$. This level is three order of lower than the regulation value of 56 Bq/$H_2-g$ from Japan. From this study, the following results were obtained. it is important that the fuel coating (SiC layer) should be kept intact to prevent the tritium from releasing. Also it is necessary that the level of impurity such as 3He and Li in the helium coolant and the reflector consisting of the graphite should be kept as low as possible. It was found that the capacity of the purification system for filtering the impurities directly from the coolant will be the important design parameter.

• Analytical Science and Technology
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• v.11 no.2
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• pp.112-119
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• 1998

A Siemens SemiQuant (SSQ) 3000 program, a precalibrated 'standardless' analytical program handling up to 90 elements, was evaluated for the fast analysis of various types of reference materials using a wavelength dispersive X-ray spectrometer. Various types of standard reference materials such as metal discs, metal chips, and geological materials in powder form were analysed and it took 23 minutes of measuring time for 75 elements. Measurements of geological reference materials using different sampling methods were carried out and their data were interactively evaluated. The analysis of materials of a known matrix concentration such as stainless steels provided higher precision value compared to totally unknown samples. The analyses of materials prepared as pressed pellets or fused glass beads provided higher precision values compared to the measurement of loose powders with a foil on the sample surface and helium operation, though their sampling procedures were more complicate and took more time. Since very light elements such as boron, carbon, and oxygen have a strong influence on the matrix effects and also on the calculation of effective matrix corrections, the rhodium Compton check was applied to verify the reliability of the defined light element concentrations of light matrix materials and the defined major sample compounds. Failure of defining correct matrix resulted in an unoptimized matrix correction and therefore in the wrong calculation of the element concentration.

• The Journal of Engineering Geology
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• v.26 no.4
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• pp.551-559
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• 2016

The chemical composition and noble gas isotopes of 10 deep groundwater samples were analyzed to know the circulation of groundwaters in the Yangsan fault and the Gampo fault. The chemical types of groundwaters show the $Ca-HCO_3$ type and $Ca-SO_4(Cl)$ type, and show indistinct relationship with geology. Noble gas isotopic data of most groundwaters were plotted along the air-crust mixing line on $^3He/^4He$ vs. $4^He/^{20}Ne$ diagram, and show dominant $^3He$ of air origin except one sample that shows helium mixing of crust origin. This indicates that groundwater actively circulates along fault, and fault could not play an role of upward pathway of a deep-seated helium gas. A comparatively high $^4He$ indicates that groundwater flows in an aquifer assuring relatively enough water-rock interaction.

• Journal of Astronomy and Space Sciences
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• v.21 no.4
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• pp.481-492
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• 2004

We report the successful fabrication and I-V curve superconductivity test results of the Nb/Al-based superconducting tunnel junctions. STJs with side-lengths of 20, 40, 60 and $80{\mu}m$ were fabricated by deposition of polycrystalline Nb/Al/AlOx/Al/Nb 5-layer thin films incorporated on a 3-inch Si wafer. STJ was designed by $Tanner^{TM}$ L-Edit 8.3 program, and fabricated in SQUID fabrication facility, KRISS. S-layer STJ thin-films were fabricated using UV photolithography, DC magnetron sputtering, Reactive ion etching, and CVD(Chemical Vapor Deposition) techniques. Superconducting state test for STJ was succeeded in 4K with liquid helium cooling system. Their performance indicators such ie energy gap, normal resistance, normal resistivity, dynamic resistance, dynamic resistivity, and quality factor were measured from I-V curve. Fabricated Nb/Al STJ shows $11\%$ higher FWHM energy resolution than genuine Nb STJ.

• Analytical Science and Technology
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• v.26 no.2
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• pp.135-141
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• 2013

The purpose of this study is to determine the quantity of selenium in grains by hydride generation-inductively coupled plasma mass spectrometry. Two sample preparation methods, beaker and microwave digestions, are compared and the former shows better result. The optimum condition for hydride generation is 4.0 M for HCl, 3% for $NaBH_4$ with the sample flow of 0.6 mL/min. The detection limit is 0.02 ${\mu}g/kg$($3{\sigma}$) and improved by 10 times. Isobaric interferences on Se is removed by Octopole Reactoin Cell and $H_2$ (3.8 mL/min) shows better performance over He. However, in case Br exists in a matrix, $H_2$ could induce interferences on m/z 80 and 82 ($^{80}[BrH]^+$ and $^{82}[BrH]^+$). The accuracy of this experiment is examined successfully by analyzing several reference materials. The results for several domestic grain analysis show that the concentrations are between 12.7 ${\mu}g/kg$ and 29.6 ${\mu}g/kg$.

• Journal of the Korean earth science society
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• v.27 no.5
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• pp.557-568
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• 2006

We present CCD UBVI photometry for more than 10,000 stars in $20'.5{\times}20'.5$ field of the halo globular cluster M30. From a color-magnitude diagram, main sequence turnoff was obtained when $V_{TO},\;(B-V)_{TO},\;and\;(V-I)_{TO}\;are\;8.63{\pm}0.05,\;0.44{\pm}0.05\;and\;0.63{\pm}0.05$, respectively. From a (U-B)-(B-V) diagram, reddening parameter, E(B-V) equals $0.05{\pm}0.01$ and a UV color excess ${\delta}(U-B)\;is\;0.27{\pm}0.01$. The abundance is derived, where [Fe/H] equals $-2.05{\pm}0.09$ according to the photometric method and spectroscopic data. The observed luminosity function of M30 shows an excess in the number of red giants relative to the number of turnoff stars, when comparing with the predictions of canonical models. Using the Hipparcos parallaxes for subdwarfs, we estimate distance modulus, $(m-M)_o\;as\;14.75{\pm}0.12$. Using the R and R' method, we find helium abundances, Y(R) as $0.23{\pm}0.02$, Y(R') as $0.29{\pm}0.02$, respectively. Finally, the cluster' sage dispersion was deduced from 10.71 Gyr to 17 Gyr.

• Journal of Navigation and Port Research
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• v.44 no.4
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• pp.283-290
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• 2020

BOG (Boil Off Gas) generation is unavoidable in the liquefied hydrogen carrier, and proper measures are necessary to prevent pressure problems inside the cargo tank. The BOG can be used as propulsion fuel for ships, and the remaining parts used for propulsion must be effectively managed, such as in the form of reliquefying or burning. This study proposes an BOG reliquefaction system optimized for a 160,000 m3 liquefied hydrogen carrier with a hydrogen propulsion system. The system comprises a hydrogen compression and helium refrigerant section, and increases the efficiency by effectively using the cold energy of the BOG discharged from the cargo tank. In this study, the system was evaluated through the exergy efficiency and SEC (Specific Energy Consumption) analysis according to the rate of the reliquefaction of the BOG while the hydrogen BOG with a supply temperature of -220℃ entered the reliquefaction system. As a result, it showed SEC of 4.11 kWh/kgLH2 and exergy efficiency of 60.1% at the rate of reliquefaction of 20%. And the parametric study of the effects of varying the hydrogen compression pressure, inlet temperature of the hydrogen expander, and the feed hydrogen temperature was conducted.

• The Journal of Engineering Geology
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• v.24 no.4
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• pp.535-550
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• 2014

The origins and varieties of natural radioactive materials, including uranium and radon-222, were examined in a drilled borehole extending to a depth of 120 m below the surface in the Goesan area. In addition to core samples, eight groundwater samples were collected at different depths, using a double packer system and bailer, and their geochemical characteristics were determined. Most of the rock samples from the drilled core consisted of granite porphyry, with sedimentary rocks (slate, carbonate, or lime-silicates) and pegmatite occurring in certain sections. The pH of samples varied from 7.8 to 8.4, and the groundwater was of a Na-$HCO_3$type. Uranium and thorium concentrations in the core were < 0.2-14.8 ppm and 0.56-45.0 ppm, respectively. Observations by microscope and an electron probe microanalyzer (EPMA) showed that the mineral containing the natural radioactive materials was monazite contained in biotite crystals. The uranium, which substituted for major elements in the monazite, appeared to have dissolved and been released into the groundwater in a shear zone. Concentrations of Radon-222 in the borehole showed no close relationship with levels of uranium. The isotopes of noble gases, such as helium and neon, would be useful for analyzing the origins and characteristics of the natural radioactive materials.