• 건강소식
• /
• v.27 no.9 s.298
• /
• pp.22-23
• /
• 2003

• The Microorganisms and Industry
• /
• v.31 no.2
• /
• pp.23-29
• /
• 2005

• Bio news
• /
• no.5
• /
• pp.16-17
• /
• 2004

• The Korean Chronic Disease News
• /
• no.58
• /
• pp.5-5
• /
• 1985

• Food Industry
• /
• s.51
• /
• pp.85-89
• /
• 1979

• Food Industry
• /
• s.41
• /
• pp.31-35
• /
• 1977

• Korean Journal of Microbiology
• /
• v.34 no.3
• /
• pp.132-136
• /
• 1998

Molecular analyses of natural microbial communities are often dependent upon the obtainments of pure nucleic acids. The four methods (elution after agarose gel electrophoresis, G-75 microspin columns, hydroxyapatite mi-crospin columns, and polyvinylpolypyrrolidone (PVPP) microspin columns) were compared for the removal of PCR-inhibitory humic substances from the crude DNA extracts of marine sediment samples. The PVPP microspin columns have shown superior removal of humic substances from the crude DNA extract of marine sediment samples, with yield of $4.8{\mu}g/g$ (dry weight of sediment). The purified DNA by this rapid method was pure enough to amplify 1.5 kb fragment corresponding almost full length of 16S rRNA genes.

• Korean Journal of Environmental Biology
• /
• v.19 no.4
• /
• pp.321-324
• /
• 2001

Groundwater samples were collected at 6 sites in Seoul area. DNA extraction from the sample was performed by the boiling method. Samples were boiled with guanidinium thyocyanate and phenol-chloroform. One set of primer was designed for amplification of 16S rDNA. For detection of denitrifying bacteria in groundwater sample, the author used primer sets consensus regions in gene sequences encoding the two forms of nitrite reductase (NIR), a key enzyme in the denitrification pathway. Two sets of PCR primer were designed to amplify $cd_1$-and Cu-nir. We confirmed the existence of denitrifying bacteria in 3 sites using $cd_1$-nir primer and in 4 sites using Cu-nir primer.

• Journal of Food Hygiene and Safety
• /
• v.18 no.1
• /
• pp.6-13
• /
• 2003

Various kinds of genetically modified organisms (GMO) and processed foods have been developed during recent years. Genetically modified organisms can be classified into several groups as their development methods. Generally, GMO has three foreign DNA regions such as gene expression adjustment region(Promoter), termination region (terminator) and structure gene. Detection of these regions can be done particularly by polymerase chain reaction (PCR). PCR-based detection can virtually be performed for any GMO within short of time. The most important prerequisite for the application of PCR-based detection is to decide abstraction method of efficient nucleic acids. Specially, in the case of processed food, because nucleic acids of foodstuffs are damaged by heat treatment (sterilization), pressure and fermentation, DNA must be extracted ken the samples prior to PCR analysis. Although many DNA extraction protocols are available, they have rarely been compared in a comprehensive method. In this study low widely used commercial and non-commercial DNA extraction methods-DNeasy$^{TM}$, Wizard$^{TM}$, CTAB, phenol/chloroform system-were compared with respect to the quality and yield of nucleic acids and insertion genes.nes.

• Korean Journal of Food Science and Technology
• /
• v.36 no.3
• /
• pp.379-384
• /
• 2004

Changes in nucleotide concentrations of aquarium-stored flounder, sea bass, and sea bream were studied. ATP, ADP, and AMP slowly decreased, whereas IMP, HxR and Hx slightly increased with increasing storage ported. ATP was converted into IMP at initial storage stage. Changes in concentrations of nucleotides differed depending on fish type and season. Freshness indicators, $K,\;K_{p}\;G,\;P,\;H,\;and\;F_{r}$ values during 14 days storage showed no significant differences. Changes in nucleotide concentrations during 14 days storage had no significant effect on taste of raw fishes.