• Korean Journal of Microbiology
• /
• v.55 no.2
• /
• pp.103-111
• /
• 2019

DNA methylation is involved in diverse processes in bacteria, including maintenance of genome integrity and regulation of gene expression. CcrM, the DNA methyltransferase conserved in Alphaproteobacterial species, carries out $N^6$-adenine or $N^4$-cytosine methyltransferase activities using S-adenosyl methionine as a co-substrate. Celeribacter marinus IMCC12053 from the Alphaproteobacterial group was isolated from a marine environment. Single molecule real-time sequencing method (SMRT) was used to detect the methylation patterns of C. marinus IMCC12053. Gibbs motif sampler program was used to observe the conversion of adenosine of 5'-GANTC-3' to $N^6$-methyladenosine and conversion of $N^4$-cytosine of 5'-GpC-3' to $N^4$-methylcytosine. Exocyclic DNA methyltransferase from the genome of strain IMCC12053 was chosen using phylogenetic analysis and $N^4$-cytosine methyltransferase was cloned. IPTG inducer was used to confirm the methylation activity of DNA methylase, and cloned into a pQE30 vector using dam-/dcm- E. coli as the expression host. The genomic DNA and the plasmid carrying methylase-encoding sequences were extracted and cleaved with restriction enzymes that were sensitive to methylation, to confirm the methylation activity. These methylases protected the restriction enzyme site once IPTG-induced methylases methylated the chromosome and plasmid, harboring the DNA methylase. In this study, cloned exocyclic DNA methylases were investigated for potential use as a novel type of GpC methylase for molecular biology and epigenetics.

• Korean Journal of Heritage: History & Science
• /
• v.50 no.1
• /
• pp.4-17
• /
• 2017

In this research, the chemical composition of the iron artifacts from the late 6th-century to early 7thcentury Baekje remains in Gwanbuk-ri, Buyeo, specifically of the nine iron artifacts including slags, furnace walls and ingot iron excavated in the District "Na", were examined by observing their chemical compounds and microstructures. As a result, GB1 and GB6 were determined to be proto-reduction lumps whereas GB2, GB3, GB4 and GB5 were determined to be tempered slags, respectively. Also, he furnace wall GB7 were containing mullite and cristobalite, which are high temperature index minerals, The extrusion temperature was found out to be about $1200{\sim}1300^{\circ}C$, and it is most likely that the smelting temperature in the furnace was in that temperature range. GB8 ingot iron was determined to be a forged ironware. This ingot iron was an intermediary product for making ironware and its nonmetallic inclusions displayed similar microstructure and contents compared to the forged iron. Because of the existence of proto-reduction lumps and forged iron, the iron making facility located in District "Na" most likely had a small-scale iron making facility that handled iron bloom smelting and refining processes.

• Korean Journal of Microbiology
• /
• v.54 no.4
• /
• pp.448-452
• /
• 2018

The full genome sequence of Bacillus safensis KCTC 12796BP which had been isolated from the marine sponge in the seawater of Jeju Island, was determined by Pac-Bio next-generation sequencing system. A circular chromosome in the length of 3,935,874 bp was obtained in addition to a circular form of plasmid having 36,690 bp. The G + C content of chromosome was 41.4%, and that of plasmid was 37.3%. The number of deduced CDSs in the chromosome was 3,980, whereas 36 CDS regions were determined in a plasmid. Among the deduced CDSs in chromosome, 81 tRNA genes and 24 rRNA genes in addition to one tmRNA were allocated. More than 30 CDSs for sporulation, 16 CDSs for spore coat, and 20 CDSs for germination were also assigned in the chromosome. Several genes for capsular polysaccharide biosynthesis and for flagella biosynthesis and chemotaxis in addition to genes for osmotic tolerance through glycine-choline betaine pathway were also identified. Above all, the biosynthetic gene cluster for anti-allergic compounds seongsanamides were found among two non-ribosomal peptide synthetase (NRPS) gene clusters for secondary metabolites.

• Journal of the Korean GEO-environmental Society
• /
• v.12 no.7
• /
• pp.23-29
• /
• 2011

The oxidation slag has been widely used in civil engineering project, whereas the reduced slag from electric furnace has yet to be applied. Consequently in order to find out the recycling method in civil engineering field, the mineral compositions of the reduced slag were analyzed and some tests on water quality were performed to estimate the potential release of toxic compounds. Slag-soil mixtures of 0, 10, 20 and 30%(dry weight) soil were prepared in lysimeter columns and the effluents were collected with the period of one, two and four week options in closed system, respectively. The result from qualitative and quantitative analysis using X-ray Diffraction(XRD) and X-ray Fluorescence(XRF) indicates that the main mineral of the reduced slag is $Ca_2(SiO_4)$, a kind of calcium silicate. Also, the leaching medium analyzed by Inductively Coupled Plasma Optical Emission Spectroscopy(ICP-OES) showed that main heavy metals such as Al, Fe and Mn are included in the reduced slag due to the effect of steel production process. It can be seen that the leachate does not violate the regulation guide line of waste material of heavy metal. Also the pH levels were increased from pH 6.9 for 0% soil to pH 10 for 30% soil. However the influence on leachate circulation period of one through four weeks was negligible.

• Journal of the Korean GEO-environmental Society
• /
• v.12 no.7
• /
• pp.31-37
• /
• 2011

Only a few studies have been conducted using reduced slag as recycled material. The reduced slag in electric furnace is produced as a by-product in making a steel and a few applications of the reduced slag as expensive additives and bonding materials or as the stabilized soils was reported. The purpose of this study is to present the feasibility of the reduced slag as recycled material, especially, in a field of civil engineering. In order to achieve the purpose experiments such as SEM and XRF analysis was conducted for the reduced slag in electric furnace. Based on the results various geotechnical experiments were conducted to know engineering properties of slag-soil mixtures. Weathered soils and clay are mixed with reduced slag for various ratios. As the ratio of reduced slag to weathered soil increases, the maximum dry unit weight of the mixture decreased with increasing optimum moisture content. The results indicates that there is no effect on a reduced slag by compaction efforts. The shear strengths of the weathered soil-slag mixtures are slightly higher or similar to those of weathered soils. The permeability of the weathered soil-slag mixtures is similar to that of silty or sandy soils. Therefore, it is possible to use the mixtures as embankment or backfill materials in the fields. The unconfined strength of the mixtures of reduced slag and clay is higher than that of clay and it tends to increase with the curing time. Therefore it can be used to improve the soft ground.

• Journal of Life Science
• /
• v.31 no.5
• /
• pp.496-501
• /
• 2021

In this study, the marine agar-degrading bacterium Pseudoalteromonas sp. JH-1 was isolated, and its growth and agarase properties were investigated. Seawater was collected from the offshore of the Yonggung Temple in Busan, and agar-degrading bacteria were isolated and cultured with marine agar medium. The bacterium Pseudoalteromonas sp. JH-1 was isolated through 16S rRNA gene sequencing. The extracellularly secreted enzyme was obtained from the culture broth of Pseudoalteromonas sp. JH-1 and was used to characterize its agarase. The extracellular agarase exhibited a maximum activity of 116.6 U/l at 50℃ and pH 6.0 of 20 mM Tris-HCl buffer. Relative activities were 31, 59, 94, 100, 45, and 31% at 20, 30, 40, 50, 60, and 70℃, respectively. Relative activities were 49, 85, 100, 86, 81, and 67% at pH 4, 5, 6, 7, 8, and 9, respectively. Residual activity was more than 85% after exposure at 20, 30, and 40℃ for 2 hr, and more than 82% after exposure at 50℃ for 2 hr. Zymogram analysis confirmed that Pseudoalteromonas sp. JH-1 produced at least two agarases of 55 and 97 kDa. As the products of α-agarase and β-agarase have antioxidation, antitumor, skin-whitening, macrophage activation, and prebiotic effects, further studies are needed on the agarase of Pseudoalteromonas sp. JH-1.

• Journal of Life Science
• /
• v.32 no.10
• /
• pp.778-783
• /
• 2022

This study isolated a new agarase-producing bacterium and characterized its agarase. A new agar-degrading strain was isolated from the seashore of Namhae in Gyeongnam province, Korea, and was purely cultured using the Marine Agar 2216 media. The isolated bacterium was identified as Simiduia sp. SH-2 after 16S rRNA gene sequencing. The crude agarase was obtained from the culture medium of the Simiduia sp. SH-2 strain, and the agar-degrading activity was measured. The highest level of activity of the Simiduia sp. SH-2-derived agar-degrading enzyme was 625 U/l. Agar degradation activity was most significant at 40℃ and pH 7.0. Compared to the activity at 40℃, the relative activity was 31% at 20℃ and 71% at 30℃. Compared to the activity at pH 7.0, the relative activity was 94% and 89% at pH 6.0 and pH 8.0, respectively. Residual activity was greater than 96% after exposure to 20℃ and 30℃ for 2 hr and more than 49% after exposure to 40℃ for 2 hr. Simiduia sp. SH-2 was identified as a strain producing β-agarase that creates neoagarooligosaccharides, such as neoagarotetraose and neoagarohexaose. Therefore, the Simiduia sp. SH-2 strain and its β-agarase are expected to be useful functional material producers in the food, cosmetic, and pharmaceutical industries.

• KSCE Journal of Civil and Environmental Engineering Research
• /
• v.42 no.6
• /
• pp.763-773
• /
• 2022

Industrial and environmental facilities, which are national growth engine, must sustain their structural safety and maintain their process to continue production activities under various load conditions including natural hazards. In this study, by improving existing design codes which aim to secure the structural safety only, new structural and seismic design codes are proposed to secure both the structural safety and the operability of facilities. In the proposed structural design code, a variety of loads to reflect the characteristics of industrial and environmental facilities are considered and load combinations for the ultimate strength design and the allowable stress design of structures are suggested. Considering the importance of a unit industrial facility and that of a unit process, the seismic design class, design earthquake, and seismic performance level of a unit component are determined to achieve the dual seismic performance objectives for securing both the structural safety and the operability. Also, the proposed design code are applied to an example of an environmental facility in order to examine its applicability.

• Resources Recycling
• /
• v.31 no.1
• /
• pp.37-45
• /
• 2022

The development of the electrical, electronic, and telecommunication industries has increased the share of electricity in total energy consumption. With the enforcement of the Act on the Promotion of the Development, Use, and Diffusion of New and Renewable Energy in 2021, the mandatory supply ratio of new and renewable energy is expected to expand, and the amount of waste cables generated in the stage of replacing and discarding cables used in the industry is also expected to increase. The purpose of this study was to quantify the environmental burden of waste cable recycling through the life cycle assessment (LCA) method. The results showed that the higher the amount of glue contained in the waste cable, the greater was the amount of fine dust and greenhouse gases generated. In addition, by assigning weights to 10 environmental burden items, it was confirmed that the marine aquatic eco-toxicity potential (MAETP) and human toxicity potential (HTP) had the greatest environmental burden. The main causes were identified as heptane and ethanol, which were the glue contained in the waste cable and the cleaning solutions used to remove them. Therefore, it is necessary to refrain from using glue in the cable production process and reduce the environmental burden by reducing the use of waste cable cleaning solutions used in the recycling process or using alternative materials.

• Tunnel and Underground Space
• /
• v.33 no.4
• /
• pp.209-227
• /
• 2023

The government continues to announce measures to revitalize smart construction technology based on BIM for productivity innovation in the construction industry. In the design phase, the goal is design automation and optimization by converging BIM Data and other advanced technologies. Accordingly, in the basic design of the Namhae Seomyeon-Yeosu Sindeok National Road Construction Project, a domestic undersea tunnel project, BIM-based design was carried out by developing tunnel design automation technology using 3D spatial information according to the tunnel design process. In order to derive the optimal alignment, more than 10,000 alignment cases were generated in 36hr using the generative design technique and a quantitative evaluation of the objective functions defined by the designer was performed. AI-based ground classification and 3D Geo Model were established to evaluate the economic feasibility and stability of the optimal alignment. AI-based ground classification has improved its precision by performing about 30 types of ground classification per borehole, and in the case of the 3D Geo Model, its utilization can be expected in that it can accumulate ground data added during construction. In the case of 3D blasting design, the optimal charge weight was derived in 5 minutes by reviewing all security objects on the project range on Dynamo, and the design result was visualized in 3D space for intuitive and convenient construction management so that it could be used directly during construction.