• Journal of the Korean Chemical Society
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• v.28 no.6
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• pp.412-417
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• 1984

A highly efficient and general route to obtain the alkyl derivatives of 2-mercaptobenzothiazole is described. Depending on the reaction conditions 2-methylmercaptobenzothiazole gave various products with chlorinating agents probably due to the lability of the probable chlorosulfonium ion intermediate.

• Analytical Science and Technology
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• v.29 no.1
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• pp.10-18
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• 2016

Phosphodiesterase (PDE) plays an important role in cAMP-mediated signaling within cells. We previously showed that the long-form of Aplysia PDE4 (ApPDE4) was localized in the plasma membrane and the presynaptic terminal in Aplysia sensory neurons, and the 16 N-terminal amino acid was sufficient for this targeting process. In this study, we characterized the cellular localization of various ApPDE4 mutants. We first identified the roles of each amino acid within the group of 16 N-terminal amino acids of long-form ApPDE4. As a result, we were able to identify various mutants that were localized to both the plasma membrane and the Golgi complex, Golgi only, or both the endoplasmic reticulum (ER) and the Golgi complex. To examine the role of palmitoylation on the cellular localization of ApPDE4 mutants, 2-bromo palmitate (2-BR) was used as a treatment. As a result, in the presence of 2-BR, the plasma membrane targeting of many mutants was impaired, indicating that palmitoylation was involved in the plasma membrane targeting of the mutants. We also found that PI4P play crucial roles in the Golgi targeting of (N16,C3S/VV/G)-mRFP, L(N16,C3S/LFS/R)-mRFP, and L(N16,EPL/R)-mRFP.

• Korean Journal of Environmental Agriculture
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• v.4 no.1
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• pp.37-42
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• 1985

Recently the method using fixing biological contactor in treatment of sewage and wastewater has been propelling on the ground that it has advantages of reducing both motive and man power and applied treatment plants of this method are increasing gradually in Korea. After analysing the results from which real structure pilot plant had operated in the field with RBC sewage wastewater system-one of the fixing biological contact methods-for five months, this study was performed to investigate how to apply the standard of establishment that provided in article of sewage disposal facilities notified (act 8 of art 84) by the office of environment on May 16, 1984, to real treatment plant. The rotating velocity and the staying time of rotating disc have interrelation on removal efficiency of BOD. When circumferential velocity of rotating disc was ranged from 18 to 20 m per minute, economical price was the best. When the staying time was even about 120 minutes for that of home RBC facilities showed 90% or above of BOD removal efficiency of high concentration sewage also showed excellent efficiency ranged from about 85% to 90%.

• Korean Journal of Materials Research
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• v.11 no.7
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• pp.585-589
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• 2001

Many experimental results have revealed that the development of cavitaition during tensile deformation is limited by the Presence of liquid phases. However, the presence of liquid phases does not always lead to high-strain-rate superplasticity, because too much liquid causes intergranular decohesion at grain boundaries/interfaces in metal-matrix composites. Thus, it is important to examine the nature of interfaces of superplastic composites in order to understand the origin of superplastic flow related to liquid grain boundaries during high-strain-rate superplastic deformation. This study shows that a large elongation is obtained at the temperature, that is close to the onset temperature for partial melting in the superplastic composites, but the elongation significantly decreases at slightly higher temperatures, which are close to the end temperature fur partial melting. This indicates that there is an optimum amount of the liquid phase for obtaining high-strain-rate superplasticity in these materials.

• Parasites, Hosts and Diseases
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• v.23 no.1
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• pp.111-122
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• 1985

The present study was performed to observe the ultrastructure of the integument of adult Paragonimus westermani. Dogs experimentally infected with 60 metacercariae of F. westermani were autopsied 4 months after the infection. Adult p. westermani were extracted from the dogs and the fine structure was studied by means of scanning and transmission electron microscope. The findings are as follows: 1. Scanning electron microscopic findings showed that the spines and the papillae are distributed at whole body surface but the well developed spines or papaillae are shown around the oral sucker and ventral sucker. 2. At the end of the body, excretory pore was found, the shape was irregular. 3. Transmission electron microscopic findings showed that plasma membrane, tegument, basal lamina, connective tissue, circular muscle layer, longitudinal muscle layer. nerve axon and tegumental cell were observed. 4. In higher magnification, plasma membrance and bar-shaped granules were found at the outer surface of the tegument.

• Microbiology and Biotechnology Letters
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• v.5 no.3
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• pp.141-151
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• 1977

Distribution of tellurite and tellurate-reducing enzymes in the cell of Nocardia sp, the purifcation and the chemical properties of enzymes were investigated. Tellurite- and tellurate-reducing enzymes were located in the cytoplasm, but T. T. C. reduction part was in the cell membrane. Purification of tellurite- and tellurate-reducing enzymes was possible with the application of ammonium sulfate precipitation method and DEAE-Cellulose or CM-Cellulose column chromatographic method from the crude soluble part of the cell. On investigating the properties of purified enzyme, one of NADP, NADPH and reductive methylene blue(leucomethylene blue) was thought to react as a hydrogen donor. Both NADH and NADPH, or either of them would be physiological hydrogen donor.) In the reaction of this enzyme, either tellurite or tellurate reacts as a hydrogen acceptor, but on the other hand either selenate or selenate also reacts as a hydrogen acceptor.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2003.07a
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• pp.213-219
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• 2003

본 연구에서는 새로운 개념의 건조방법을 연구하여 곡물건조의 변화를 도모하고자 원적외선ㆍ열풍 복합열을 이용한 곡물건조기를 개발하게 되었다. 그 결과를 요약하면 다음과 같다. 가. 방사체길이가 1,680mm일 때 보다 1,470mm일 때가 방사체 표면온도가 높게 나타났고, 안정적인 것으로 나타났다. 열풍온도를 5$0^{\circ}C$로 설정했을 때 방사체의 표면온도분포는 280-29$0^{\circ}C$을 유지하였고, 6$0^{\circ}C$일 때는 30$0^{\circ}C$ 부근에서 유지되는 것을 확인할 수 있었다. 두 조건 모두에서 온도편차는 크게 나타나지 않았으므로 균일 건조가 이루어지는 것을 의미하며, 곡물의 품질저하에 영향을 미치지 않는 것으로 판단된다. 나. 열풍온도 5$0^{\circ}C$, 조사거리 125mm, 방사체 길이 1,470mm에서 방사체 길이방향으로 위치에 따라서 온도편차를 측정했을 때 버너를 기점으로 해서 근거리에서부터 원거리까지 균등 분할하여 5점의 온도를 측정하여 그 변화곡선을 분석한 결과 위치 3에서 온도가 높았고, 계속해서 위치 4, 5, 2, 1순으로 나타났다. 버너의 근거리에서보다 원거리에서 온도가 높게 나타난 것은 원적외선방사체를 통과하는 열풍이 빠져나가도록 되어있는 열풍유동관이 버너 원거리에 위치하고 있어 버너에 불꽃이 점화되면서 열풍이 방사체 끝쪽으로 유동되기 때문이다. 다. 건조실 수직면 길이방향의 온도는 열풍공기가 열풍실에서 유입되는 하단부이 온도가 높게 나타났고, 버너쪽과 송풍기쪽의 온도차는 나타나지 않아 온도분포의 좌우 대칭이 잘 되어 균일 건조가 되는 것으로 판단된다 이러한 현상은 건조실의 수평면에 대해서도 같은 현상이 나타났다. 라. 바닥면에서 상부로 올라갈수록 낮은 온도분포를 나타내고 있는 것은 상부에는 외부공기가 유입되면서 온도가 떨어지는 반면 하부에는 외부공기 유입이 적기 때문으로 사료된다. 또한 열풍실의 길이방향 위치별 온도 분포에서도 같은 현상으로 나타났고, 버너쪽과 송풍기쪽의 온도편차는 나타나지 않아 균일 건조를 기대할 수 있다. 마. 열풍온도를 45$^{\circ}C$로 설정하고 조사거리와 방사체 길이를 각각 119, 1,470mm로 하여 벼의 건조성능시험을 열풍건조기(대비구)와 비교시험 결과 시험구에서 건감률, 건조소요에너지가 각각 0.58%(w.b.), 470kcal/kg - water로 대비구보다 각각 건감율은 23% 높았고, 건조소요에너지는 2%의 절감되었다. 바. 건조기에서 발생되는 소음은 버너쪽 근처에서는 대비구 94.12㏈의 87%에 불과하였으나, 거리가 멀어질수록 차이는 크지 않았다. 이것은 버너에서 멀어질수록 외부적인 요인이 소음에 영향을 미쳤기 때문인 것으로 생각된다. 사. 시작기와 대비구간의 경제성에서 시작기의 구입가격이 20% 비싸기 때문에 시간당 고정비가 높았으나, 건조성능이 우수하여 건조비용이 69,350원/톤으로 대비구보다 14% 절감되는 것으로 나타났다.

• Elastomers and Composites
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• v.14 no.3
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• pp.161-168
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• 1979

이 논문(論文)의 목적(目的)은 열적안정성(熱的安定性) 및 내노화성면(耐老化性面)에 있어서 디엔제3성분단위체(第三成分單位體)(diene termonomer)의 각각(各各)의 종류(種類)가 EPDM의 중합체(重合體)의 성질(性質)에 미치는 효과(效果)를 구명(究明)하는데 있다. 제3성분단위체(第三成分單位體) 5종(種)으로 각각(各各) 다음과 같다. 즉, ethylidene norbonene(ENB), butadiene(BD), dicyclopentadiene(DCPD), methyltetrahydroindene (MTHI) 및 1,4-hexadiene(HD)이다. 이들을 써서 만든 각각(各各)의 EPDM은 동(同)몰의 불포화도(不飽和度)로 만들어졌다. 또한 가황계(加黃系)는 동일(同一)한 황/촉진제계(黃/促進劑系)를 사용(使用)하였다. ENB-EPDM이 순(純)고무배합체(配合體) 및 충전제함유배합체(充塡劑含有配合體)의 가황(加黃)에 있어서 모두 함께 가장 빠른 가황속도(加黃速度)를 보였다. HD-EPDM은 순(純)고무배합체(配合體)에서 가황속도(加黃速度)가 가장느렸으나 충전제함유배합물(充塡劑含有配合物)에서는 DCPD-EPDM보다는 발랐다. BD-EPDM을 제외(除外)한 이들 중합체(重合禮)는 거의 같은 초기가교밀도(初期架橋密度)를 갖는다. 가교밀도(架橋密度) 및 가교형(架橋型)을 분석(分析)하여 보면 BD-EPDM 쇄(鎖)에서 부타디엔 단위(單位)는 블럭을 이루고 있다. 또한 HD-EPDM은 순(純)고무가황체(加黃體) 및 충전제배합가황체(充塡劑配合加黃體)에 있어서 원가교결합(原架橋結合)의 50%가 monosulfide의 구조(構造)를 가지고있다. 이외(外)의 4종(四種)의 EPDM 폴리머는 보다 낮은 monosulfide구조를 가진다. $177^{\circ}C(350^{\circ}F)$의 노화온도(老化溫度)에서 ENB 및 HD폴리머는 약(約) 65% monosulfide 가교(架橋) 및 거의 동일(同一)한 파괴에너지값$(E_b)$을 가진다. 그러나 1,4HD의 원가교(原架橋)의 monosulfide 구조함량(含量)이 보다 높다고 해서 그의 내노화성(耐老化性)이 다른 폴리머보다 더 좋다고는 생각되지 않는다. DCPD는 $177^{\circ}C(350^{\circ}F)$의 노화온도(老化溫度)에서 똑같은 monosulfide가교(架橋)를 가지나 노화온도(老化溫度)가 $259^{\circ}C(500^{\circ}F)$로 높아짐에 따라 monosulfide 함량(含量)도 증가(增加)한다. $550^{\circ}F(287.7^{\circ}C)$의 노화온도(老化溫度)에서는 EPDM폴리머의 모든 가교(架橋)가 monosulfide구조가 되나 전가교밀도(全架橋密度) 및 $E_b$ (신장률(伸長率), 절단시(切斷時)의)는 대단(大端)히 낮은 것으로 나타나는데 이것은 산화(酸化)에 의한 노화(劣化)에 기인(基因)되는 것으로 보인다. 질소기류(窒素氣流)속에서의 TGA의 분석결과(分析結果)를 보면 EPDM 가황체(加黃體)는 $800\sim935^{\circ}F(427\sim502^{\circ}C)$의 온도범위(溫度範圍)에서 분해(分解)되며 공기중(空氣中)에서는 $750\sim935^{\circ}F$ 범위(範圍)에서 분해(分解)한다.

• Korean Journal of Microbiology
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• v.42 no.1
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• pp.19-25
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• 2006

This study was undertaken to develop PCR primers for the identification and detection of Streptococcus mutans (by)using species-specific forward and universal reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene (rDNA). The primer specificity was tested against 11S. mutans strains and 10 different species (22 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. mutans ATCC $25175^T$. The data showed that species-specific amplicons were obtained from all the S. mutans strains tested, which was not observed in the other species. The direct and nested PCR could detect as little as 2 pg and 2 fg of the chromosomal DNA from S. mutans ATCC $25175^T$, respectively. This shows that the PCR primers are highly sensitive and applicable to the detection and identification of S. mutans.

• Korean Journal of Medicinal Crop Science
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• v.8 no.1
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• pp.41-48
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• 2000

This study was aimed to proliferate Gentiana axillariflora Leveille which is one of the important medicinal and ornamental plants, by establishment of multiple shoot formation and embryogenesis through tissue culture technique. Callus was formed on MS (Murashige and Skoog) medium supplemented with 2,4-D, CPA, but not formed with BAP. The addition of 2,4-D 2 mg/ l into the medium was effective for callus formation and the rate of callus formation was about 90%. Somatic embryos were obtained on MS medium for two months. When callus was cultured on MS medium with combination treatment of 2,4-D 0.5 mg/ l and BAP 0.5 mg/ l, the number of embryo formed was better than that of other single or combination treatments and the total numbers of embryo a were 18.8 (number of total embryo/number of explants incubated = 753/40) at mean. Callus induction from stem and node explants was increased by addition of TDZ 2 mg/ l in the presence of 2,4-D 2 mg/ l, respectively. The best result about the differentiation of shoots was obtained on MS medium added BAP 2 mg/ l from node culture. Multiple shoots from shoot apex were induced on MS medium containing BAP 1 mg/ I and TDZ 1 mg/ l , BAP 2 mg/ l and TDZ 1 mg/ l. The number of multiple shoots per one explant was above seventy plants. It was the most effective regeneration system for rapid multiplication of Gentiana axillariflora Leveille.