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Nested PCR for the Detection of Streptococcus mutans  

Choi, Min-Ho (Department of Prosthodontics, College of Dentistry, Chosun University)
Yoo, So-Young (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Lim, Chae-Kwang (Institute of Forensic Odontology, College of Dentistry, Chosun University)
Kang, Dong-Wan (Department of Prosthodontics, College of Dentistry, Chosun University)
Kook, Joong-Ki (Department of Oral Biochemistry, College of Dentistry, Chosun University)
Publication Information
Korean Journal of Microbiology / v.42, no.1, 2006 , pp. 19-25 More about this Journal
Abstract
This study was undertaken to develop PCR primers for the identification and detection of Streptococcus mutans (by)using species-specific forward and universal reverse primers. These primers targeted the variable regions of the 16S ribosomal RNA coding gene (rDNA). The primer specificity was tested against 11S. mutans strains and 10 different species (22 strains) of oral bacteria. The primer sensitivity was determined by testing serial dilutions of the purified genomic DNA of S. mutans ATCC $25175^T$. The data showed that species-specific amplicons were obtained from all the S. mutans strains tested, which was not observed in the other species. The direct and nested PCR could detect as little as 2 pg and 2 fg of the chromosomal DNA from S. mutans ATCC $25175^T$, respectively. This shows that the PCR primers are highly sensitive and applicable to the detection and identification of S. mutans.
Keywords
16S rDNA; PCR primer; Streptococcus mutans;
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