• Korean Journal of Communication Studies
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• v.18 no.4
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• pp.31-50
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• 2010

This study is designed to decode the persuasion strategies in the advertising targeted for children from a semiotic perspective. Semiotic approach to "decoding advertising text" allows us to classify advertising signs and sign systems in relation to the way they are transmitted and communicated. In this strategic process of advertising production, the producers use various "expressive techniques" and "persuasive appeals, premises, and ideology" shared by the members of the society. This study investigates Korean print media advertising for children with the following research questions: ① What types of expression techniques are employed in the print media advertising for children? ② What types of persuasive appeals(consumption values) are used in the print media advertising for children? ③ What are the persuasive premises(cultural premises) underlying those persuasive appeals? ④ What is the structure of ideology that governs those persuasive appeals and premises in the advertising for children? The study result reveals seven most frequently employed expressive techniques, ten major types of persuasive appeals and premises, and the structure of ideology governing the signs and signification systems in the advertising targeted for children.

• Proceedings of the KIEE Conference
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• 2008.10b
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• pp.266-267
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• 2008

ERP(Enterprise Resource Planning) 및 그룹웨어 등의 전자적인 메일, 결재, 문서 관리 수단의 발달에도 불구하고 금융 및 산업계의 프린팅 수요는 매년 급증하고 있다[1]. 특히 근래의 프린터는 스캐닝, 복사, 프린팅 보안 시스템 등의 다양한 기능들이 융복합화 하면서 사무실에서의 문서 수요와 관리를 책임지는 시스템으로 진화하였다[2]. 다양한 문서 포맷에 대한 프린터의 직접 지원도 ubiquitous의 시나리오에 필수적인 아이템이 되어 가고 있다[2]. 이에 본 논문에서는 인터넷 공간에서 가장 널리 쓰이는 문서 포맷의 하나인 Adobe사 PDF(Portable Document Format) 문서 포맷을 중심으로 프린터에서 직접 문서 해독 및 이미지 처리와 렌더링을 수행하는 S/W 시스템에 대해 그 아키텍쳐와 구현 예를 보이고자 한다. 아울러 임베디드 시스템에서 direct printing S/W의 고려 사항에 대해 논하고자 한다.

• Journal of Industrial Technology
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• v.28 no.B
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• pp.53-57
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• 2008

From the extensive screening for small cryptic plasmid among about 23 lactic acid bacteria (LAB), 2.4 kb of cryptic plasmid was isolated from Lactobacillus farciminis strain KCTC 3681 and named as pLF24. The plasmid pLF24 was a circular molecule of 2,396 base-pairs in length with a G+C content of 38%. Two protein-coding sequences could be predicted. ORF1 and ORF2 showed homologies to plasmids of gram-positive bacteria. The replication protein coded by ORF2 and the plus origin, were similar to replication regions of other gram-positive bacteria as shown in plasmids such as pLH2, pLS141-1 and pLC2. The nucleotide sequence of pLF24 was deposited into Genbank data base with an accession number of EU429343. The newly isolated plasmid can be used for construction of shuttle vector in Lactobacillus bacteria.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.18-24
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• 1999

Kinesin has been discovered in Saccharomyces cerevisiae, Aspergillus nidulans, and Drosophila melanogaster and it has major roles in the movemenl of chromosomes and separation of spindle poles. In this study, a gene encoding kinesin heavy chain in Schizosaccharo~n)~ces pombe was cloned by using the polymerase chain reaction with degenerated primcrs corresponding to highly conserved regions of the kinesin heavy chain motor domain. The kinesin gene in S pombe contains an open reading frame of 2496 base pairs and encodes a kinesin prolein of 832 amino acids with a molecular weight of 96 kd. From thc comparison of the predictcd amino acids of the newly cloned kinesin, the kinesin in S. pornbe belongs to the C-terminal motor subfamily of kincsin-related protein.

• Journal of Life Science
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• v.22 no.3
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• pp.306-311
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• 2012

A gene encoding a lipase, lipT, was cloned from the psychrotrophic bacterium Pseudomonas mandelii and sequenced. An open reading frame of 1,686 bp was found that encodes a polypeptide consisting of 562 amino acid residues. Sequence analysis revealed a Gly-His-Ser-Leu-Gly sequence, which matches the consensus Gly-X-Ser-X-Gly motif conserved among lipolytic enzymes. The recombinant LipT protein was predominantly expressed as inclusion bodies in Escherichia coli and subsequently purified by nickel-chelate affinity chromatography. A small fraction of LipT was refolded, and the subsequent LipT exhibited substrate preferences for p-nitrophenyl butyrate (C4) and p-nitrophenyl octanoate (C8).

• Proceedings of the Korea Society for Industrial Systems Conference
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• 2000.05a
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• pp.101-106
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• 2000

자동화 기기 분야에서 컴퓨터의 적용 및 응용은 하드웨어 발달에 따라 매우 빠르고 민감하게 반영되어왔다. 이는 컴퓨터 하드웨어의 빠른 개발 주기에 맞춰 저 가격, 고 효율성, 높은 신뢰성, 호환성 등의 장점을 가진 PC가 현대의 컴퓨터 흐름을 주도하게 되면서 자동화 산업분야 또한 이를 적용하여 왔기 때문이다. 이에 따라, 자동화 기기 분야에서는 고 가격, 긴 개발기간 등을 필요로 했던 과거와 달리 저 가격, 짧은 개발기간, 다양한 개발환경 등을 이룰 수 있었다. 또한, 생산량 증가에만 의존하던 과거와 달리 현대에 이르러서는 시스템의 최적화, 효율의 극대화, 시스템의 안정성, 운용의 편리성, 호환성 등의 개념들이 도입되고 있는 것이다. 자동화 기기를 구성하는 요인으로는 크게 시스템의 틀을 이루는 기계부분과 이를 제어하는 제어 시스템부로 나뉠 수 있다. 제어 시스템에서는 기계부분의 동작을 제어하는 동작 제어부와 이에 관한 정보를 화면에 나타내는 GUI(Graphical User Interface)부분으로 나뉘게된다. 현재에는 이를 통합하여 하나의 하드웨어에서 제어부와 GUI를 모두 담당하는 방법이 연구 진행되고 있으나, 하드웨어를 둘로 나누거나 하나로 하여도 제어부와 GUI 사이의 통신부분은 빼놓을 수 없는 요소가 된다. 따라서, 본 논문에서는 시스템의 안정성을 위하여 두 시스템간에 송·수신되는 데이터를 추적할 수 있도록 하는 Emulate 기법을 구현 및 개발하고자 한다. 이는, 두 시스템간의 통신 데이터를 실시간으로 누적, 저장하여 사용자로 하여금 시스템의 운용상태를 분석할 수 있게 하였으며, 시스템 오류발생 시 Emulate 자료를 근거로 시스템의 운용상태를 파악할 수 있게 하였다.근 제한기능을 제공하며 각 클라이언트와 서버간의 실시간 연결 혹은 지연연결을 지원하는 독립적인 애플리케이션이다. 이러한 처방전달 메시징시스템을 구성하는 각 요소에 대해 정의하고 개념적 모델을 설계하고자 한다.에게 청구되며, 소비자에게 전송 되는 청구서는 사용자DB를 참조하여 사용자가 미리 정의한 원하는 형태로 변환되어 전달되며, 필요시 암호화 과정을 거치는 것이 가능해야 한다. 전송된 청구서는 전자우편의 경우, 암호해독이 가능한 전용 브라우저를 통해 열람 되며, 이는 다시 전용 브라우저를 통해 지불인증이 승인되어 청구 제시서버에게 전송된다. EBPP 시스템의 제어 흐름은 크게 기업이 청구 정보를 소비자에게 제시하는 흐름과 소비자의 지불 승인으로 인해 기업이 은행에 지불을 요구하는 흐름으로 구분할 수 있다. 본 논문에서는 통합 청구서버 및 정구 제시서버의 역할 및 구성 요소들에 대해 서술하고, EBPP 시스템과 연동하여야 하는 메일 서버와의 상호 작용에 대해 서술할 것이다. 본 시스템을 아직 구현이 되지 않은 관계로 시스템의 성능 등의 수치적 결과를 제시할 수 없는 상태다., 취약계층을 위한 일차의료, 의약관리), ${\circled}2$ 보건소 조직 개편 및 민간의료기관과 협력체계 확립, ${\circled}3$ 전문인력 확보 및 인력구성 조정, 그리고 ${\circled}4$ 방문보건사업의 강화 등이다., 대사(代謝)와 관계(關係)있음을 시사(示唆)해 주고 있다.ble nutrient (TDN) was highest in booting stage (59.7%); however no significant difference was foun

• Journal of Life Science
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• v.17 no.9 s.89
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• pp.1197-1203
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• 2007

A genomic DNA library of the bacterium Paenibacillus sp. DG-22 was constructed and the ${\beta}-xylosi-dase-positive$ clones were identified using the fluorogenic substrate $4-methylumbelliferyl-{\beta}-D-xylopyr-anoside$ $({\beta}MUX)$. A recombinant plasmid was isolated from the clone and 4.3-kb inserted DNA was sequenced. The ${\beta}-xylosidase$ gene (xylA) was comprised of a 2,106 bp open reading frame (ORF) en-coding 701 amino acids with a molecular weight of 78,710 dalton and a pI of 5.0. The deduced amino acid sequence of the xylA gene product had significant similarity with ${\beta}-xylosidases$ classified into family 52 of glycosyl hydrolases. The xylA gene was subcloned into the pQE60 expression vector to fuse with six histidine-tag. The recombinant ${\beta}-xylosidase$ $(XylA-H_6)$ was purified to homogeneity by heat-treatment and immobilized metal affinity chromatography. The pH and temperature optima of the $XylA-H_6$ enzyme were pH 5.5-6.0 and $60^{\circ}C$, respectively.

• Radiation Oncology Journal
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• v.17 no.2
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• pp.151-157
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• 1999

Purpose : By sequencing the Erpressed Sequence Tags of human 걸ermal papilla CDNA library, we identified a clone named K872 of which the expression decreased during differentiation of HL6O cell line. Materials and Methods : K872 plasmid DNA was isolated according to QIA plasmid extraction kit (Qiagen GmbH, Germany). The nucleotide sequencing was performed by Sanger's method with K872 plasmid DNA. The most updated GenBank EMBL necleic acid banks were searched through the internet by using BLAST (Basic Local Alignment Search Tools) program. Nothern bots were performed using RNA isolated from various human tissues and cancer cell lines. The gene expression of the fusion protein was achieved by His-Patch Thiofusicn expression system and the protein product was identified on SDS-PAGE. Results : K872 clone is 1006 nucleotides long, and has a coding region of 675 nucleotides and a 3' non-coding region of 280 nucleotides. The presumed open reading frame starting at the 5' terminus of K872 encodes 226 amino acids, including the initiation methionine residue. The amino acid sequence deduced from the open reading frame of K872 shares $70\%$, identity with that of rat glutathione 5-transferase kappa 1 (rGSTKl). The transcripts were expressed in a variety of human tissues and cancer cells. The levels of transcript were relatively high in those tissues such as heart, skeletal muscle, and peripheral blood leukocyte. It is noteworthy that K872 was found to be abundantly expressed in coloreetal cancer and melanoma cell lines. Conclusion : Homology search result suggests that K872 clone is the human homolog of the rGSTK1 which is known to be involved in the resistance of cytotoxic therapy. We propose that meticulous functional analysis should be followed to confirm that.

• Journal of the Korean Geographical Society
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• v.29 no.4
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• pp.438-449
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• 1994

This paper focuses on recontextualizing geography curriculum, i.e. examining recent changing aspects in three geography curriculum locators-society, student and discipline of geography-and searching future directions of geography curriculum in light of such changes. For conciliation and reflection of changing aspects of each locators, this paper dealt with social issues and societal changes in terms of locator of society, increased concern to student and development of cognitive science in terms of students, and challenging views on science and the meaning of epistemological changes in geography in terms of discipline. As a result, three future directions in geography curriculum are searched : issue-based geography curriculum, thinking geography curriculum, geography curriculum toward equity and accessbility.

• Journal of Genetic Medicine
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• v.4 no.1
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• pp.38-44
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• 2007

Purpose : Menkes disease is an X-linked recessively inherited disorder caused by the mutation of the ATP7A gene encoding copper-transporting P-type AT Pase. The phenotypic features are progressive neurological degeneration, mental retardation, loose skin, and vascular complications. Early diagnosis and treatment are very important for the prognosis of Menkes disease. Here, we describe nov el mutations of the ATP7A gene and prenatal diagnosis by mutation analysis. Methods : Five unrelated Korean Menkes patients were included in this study. They presented with depigmented wool-like hair, progressive neurologic deterioration, and hypotonia in infancy. Serum copper and ceruloplasmin levels w ere decreased. Brain magnetic resonance imaging revealed tortuous intracranial vessels. Mutation analysis has been carried out using cDNA from cultured skin fibroblasts or genomic DNA from peripheral leukocytes. Prenatal diagnosis was performed in two cases using chorionic villi samples or amniocytes. Results : Four novel mutations have been identified from four different families; c.3511+1G>A (p.E1099_N1171delinsMfsX 18), c.4005+5 G>A (p.V1268_R1335del), c.1870_2172del (p.S624_Q724del), and c.3352 G>A (p.G1118S). T he remaining one was previously reported (c.1933 C>T (p.V 1268_R1335del)). On prenatal DNA analysis, one w as diagnosed as normal, while the other turned out to be a female heterozygote with p.S624_Q724del mutation of the ATP7A gene. Conclusion : We identified 4 novel mutations of the ATP7A gene. Prenatal diagnosis in families at risk is critical in order to choose preventiv e options including an early treatment with copper-histidine therapy or therapeutic termination. Most mutations of the ATP7A gene were frame-shift mutations and prenatal diagnosis has been successfully carried out.