• Title/Summary/Keyword: 항온시험

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Fluorescence Assay for High Efficient Mass Screening of the Herbicides Inducing Rapid Membrane Peroxidation (막과산화를 신속히 유발하는 제초제의 고효율 대량스크리닝을 위한 형광검정법)

  • Kim, Jin-Seog;Kwon, Ok Kyung
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.308-314
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    • 2015
  • This study was conducted to establish a fluorescence assay system for high efficient mass screening of the herbicides causing rapid membrane peroxidation, based on the fact that peroxide in cellular leakage could be fluorometrically determined through the fuorescent compounds formed after reacting with homovanillic acid (HVA) and peroxidase (HRP). The assay procesure established in this study was as follows. Only single disc (4 mm diameter) excised from cucumber cotyledon is placed on the well containing test solution ($200{\mu}L$) with 96-well microplate. The plate is shaking-incubated for 8 h under light condition. Then after removing the cucumber disc, HVA and HRP are supplied in the medium buffer and incubated for 5 min at room temperature. Fluorescence values are determined at Ex 320 nm/Ex 425 nm. The higher fluorescence values are obtained in the treatment of chemical having higher herbicidal activity. Using this assay with 96-well microplates, a large number of herbicides inducing rapid membrane peroxidation seemed to be screened more efficiently than spectrophotometric microtiter assay reported previously.

Influence of Polarization Behaviors on the ECM Characteristics of SnPb Solder Alloys in PCB (PCB에서의 ECM 특성에 미치는 SnPb 솔더 합금의 분극거동의 영향)

  • Lee Shin-Bok;Yoo Young-Ran;Jung Ja-Young;Park Young-Bae;Kim Young-Sik;Joo Young-Chang
    • Journal of the Microelectronics and Packaging Society
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    • v.12 no.2 s.35
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    • pp.167-174
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    • 2005
  • Smaller size and higher integration of electronic components make smaller gap between metal conducting layers in electronic package. Under harsh environmental conditions (high temperature/humidity), electronic component respond to applied voltages by electrochemically ionization of metal and metal filament formation, which lead to short failure and this phenomenon is termed electrochemical migration(ECM). In this work, printed circuit board(PCB) is used for determination of ECM characteristics. Copper leads of PCB are soldered by eutectic solder alloys. Insulation breakdown time is measured at $85^{\circ}C,\;85{\%}RH$. CAF is the main mechanism of ECM at PCB. Pb is more susceptible to CAF rather than Sn, which corresponds well to the corrosion resistance of solder materials in aqueous environment. Polarization tests in chloride or chloride-free solutions fur pure metal and eutectic solder alloys are performed to understand ECM characteristics. Lifetime results show well defined log-normal distribution which resulted in biased voltage factor(n=2) by voltage scaling. Details on migration mechanism and lifetime statistics will be presented and discussed.

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Distributional Data and Ecological Characteristics of Parnassius bremeri Bremer in Korea (붉은점모시나비의 국내 분포정보 및 생태적 특성 조사)

  • 고민수;이준석;김철학;김성수;박규택
    • Korean journal of applied entomology
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    • v.43 no.1
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    • pp.7-14
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    • 2004
  • This study was carried out to survey and confirm the occurring sites of Parnassius bremeri in Korea, and to investigate ecological characteristics to develop a mass rearing technique. In the field survey, adults were found in the two previously known sites in Gyungnam Province and another site was newly found in Samcheok, Gangwon Province. Emergence period of adults was from middle of May to middle of June. Oviposition took place on various material, including hostplant, debris, dead leaves, etc. Eggs were laid singly, up to 126.7 eggs per female. The egg-period was 221.3${\pm}$2.3 days, eggs were hatched from 11th to 22nd of January in the natural condition, and started to feed for about 10 days after hatching. Survival rate of the 1st larvae was 67.6%. Developing period of each instar in the insectary (25$^{\circ}C$, 75% RH, 16L:8D) was 11.2 days for the 1st instar, 7.3 days for 2nd, 12.8 days for 3rd, 16.2 days for 4th, and 18.2 days for 5th, and the pupal period was 21.3 days. The average longevity of adults was 26.2 days. Oviposition rate was higher in the natural condition with enough space to fly for 3♀ : 1 ♂ coupled, at least 3 ${\times}$ 3${\times}$4 m-sized room, than in smaller cage. In a comparison of the preference for visiting on sugar source, black sugar and fructose were effective.

Verification of Durability of Electromagnetic Metamaterial Absorber in Temperature Varying Environment for Its Application to Integrated Mast of Next-Generation Destroyer (차기구축함 통합마스트에 적용을 위한 전자기파 메타물질 흡수체의 온도 환경 내구성 검증)

  • Ra, Young-Eun;Kim, Yongjune;Jung, Hyun-June;Park, Pyoungwon;Jo, Jeongdai;Lee, Joonsik;Kim, Myungjoon;Jung, Joonkyo;Lee, Gun-Min;Lee, Jong-Hak;Lee, Hak-Joo
    • Journal of IKEEE
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    • v.24 no.1
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    • pp.347-353
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    • 2020
  • In this paper, the durability of an electromagnetic metamaterial absorber is verified in a temperature varying condition mimicking a maritime environment for the purpose of applying it to reduce the radar cross section of an integrated mast of the next-generation destroyer. To validate the durability, the reflectance of the electromagnetic metamaterial absorber was measured after storing it in a chamber that can control the temperature according to Procedure I of Method 501.7 included in MIL-STD-810H. Before and after the environmental test, both of the measured reflectances were retained less than -10 dB over the X band, that can guarantee the stealth functionality.

Characteristics of Predation of Neoseiulus fallacis (Acarina: Phytoseiidae) on Panonychus citri (Acari: Tetranychidae) (귤응애에 대한 팔라시스이리응애의 포식특성)

  • Kim, Dong-Hwan;Kim, Sang-Soo;Kim, Kwang-Sik;Hyun, Jae-Wook
    • Korean journal of applied entomology
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    • v.45 no.2 s.143
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    • pp.145-152
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    • 2006
  • Predation of Neoseiulus fallacis was observed for biological control of Panonychus citri that is one of the major insect pests on citrus. The daily predation of development stages of P. citri by an adult female of N. fallacis were 20.1 eggs, 26.1 larvae, 18.2 protonymphs, and 7.4 deutonymphs at 25$^{\circ}C$, The daily predation of P. citri eggs by N, fallacis was observed under different temperatures. The predation was increased as the temperature rise. At this time, ratio of eggs production of l! fallacis after predation of P. citri eggs (number of eggs N. fallacis/number of eggs P. citri consumed by N. fallacis) was 0.09. The daily predation of P. citri eggs by N, fallacis was 21.1, 17.3, and 16.7 on the different arenas (diameter: 20, 40, and 60 mm), respectively. The predation was decreased as the arena of the leaf increase. The functional response of M fallacis to P. citri showed Holling's Type II response: the consumption of prey by N. fallacis increased as the density of prey increase but increasing rate was gradually reduced. As the result, it seemed that N. fallacis can be use for biological control of P. citri.

Change of shear bond strength of orthodontic brackets according to surface treatment on dental gold alloy (치과용 금합금의 표면처리에 따른 교정용 브라켓의 전단결합강도 변화)

  • Min, Ji-Hyun;Hwang, Hyeon-Shik;Kim, Jong-Chul
    • The korean journal of orthodontics
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    • v.30 no.4 s.81
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    • pp.483-490
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    • 2000
  • The dental gold alloy shows a lower bond strength than the natural teeth in bracket bonding, and this can be a possible source of subsequent bond failure. This study aims to evaluate the effect of various gold alloy surface treatment techniques on shear bond strength between the orthodontic adhesives and the gold alloy and to find ways of increasing the bond strength. Two hundred and forty specimens made of the dental fold alloy were divided into twelve groups based on the combination of surface treatment methods(non-surface treatment, sandblasted, sandblasted plus tin-plated, and sandblasted plus intermediate adhesive) and adhesive systems (Ortho-one, Panavia 21, Superbond C&B). The specimens with bonded brackets were placed in distilled water at $37^{\circ}C$ for 24 hours and shear bond strength was measured by a universal testing machine. The results were as follows: 1. All surface-treated groups showed a significantly higher shear bond strength than non-surface-treated groups. 2. The sandblasted plus tin-plated group showed a significantly higher shear bond strength than the sandblasted group only when Panavia 21 was involved. 3. The sandblasted plus intermediate adhesive group showed a significantly higher shear bond strength than sandblasted group regardless of the type of adhesive used. 4. Of the three resin adhesive types, the Superbond C&B showed the highest bond strength, followed by Panavia 21 and Ortho-one. These findings suggest that a combination of sandblasting and intermediate resin treatment is desirable in order to enhance bracket bond strength regardless of adhesive types.

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Isolation of Alfalfa Nodule Bacteria ana Assessement of their Nitrogen Fixing Capacities (알팔파근류균(根瘤菌)의 분리(分離) 및 질소고정능력(窒素固定能力)의 비교(比較))

  • Choi, Woo-Young;Kim, Seung-Yeol
    • Applied Biological Chemistry
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    • v.22 no.3
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    • pp.166-172
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    • 1979
  • A series of experiments was planned for practical application of rhizobia in grass lands in Korea. This is the report for the studies mainly on the isolation and characterization of alfalfa nodule bacteria, and on the assessment of their nodulation abilities and nitrogen fixation capacities. 1. Total number of 47 strains was isolated from nodules which were taken from alfalfa grown in Daekwanyong, Cheju and other places. 2. Morphological and cultural characteristics of the strains were studied, and attempts. were also made to investigate their antigenic properties and to demonstrate lysogenic strains. The results were; i) the isolates varied in their cultural characteristics on yeast mannitol broth and agar, and in degree of congo red absorption; ii) similarities in their antigenic prorerties were found between the strains: SU 47/M-11, M-13/M-15, and M-3/M-5; iii) no lysogeny was found in the strains. 3. Plant infection test by test tube method in light room were carried out to elucidate the ability of the strains to nodulate Luna alfalfa and of the capacity of such nodules to fix atmospheric nitrogen. The isolates were grouped info non-invasive, ineffective, or effective to the legumes. Those strains which produced effective nodules, supporting similar/higher level of growth as nitrate control were: M-8, 9, 14, 20, 21, 25 and 34.

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The Effects of Fermented Anchovy on the Bromobenzene-Induced Hepatic Lipid Peroxidation in vitro (시험관내에서 멸치액젓이 Bromobenzene유발 간조직 지질과산화에 미치는 효과)

  • Park, Jong-Ok;Choi, Jong-Won;Kim, Hee-Sook;Ryu, Byung-Ho
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1179-1185
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    • 2000
  • Fermented anchovy was used to investigate its effects on the formation of lipid peroxide and the activities of epoxide or free radical generating enzyme in vitro in bromobenzene-treated rats. All solvent fractions from fermented anchovy not only showed the strong antioxidative activities on linoleic acid autooxidation, but also reduced bromobenzene-induced hepatic lipid peroxidation. The activities of aniline hydroxylase and aminopyrine N-demethylase elevated by bromobenzene were recovered to the level of normal rats by adding the solvent fractions of fermented anchovy. But, xanthine oxidase and aldehyde oxidase activities were not affected by fermented anchovy. These results suggest that reduction of the bromobenzene-induced hepatic lipid peroxidation is caused by inhibition on the epoxide formation, not on free radical generation.

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Analysis of the behavior of microorganisms isolated from the medium during cultivation of Agaricus bisporus (button mushroom) (양송이 재배 중 배지에서 분리한 미생물의 상호작용 분석)

  • Min, Gyeong-Jin;Park, Hae-sung;Lee, Eun-Ji;Yu, Byeong-kee;Lee, Chan-Jung
    • Journal of Mushroom
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    • v.19 no.2
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    • pp.103-108
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    • 2021
  • This experiment investigates the characteristics of microorganisms isolated from a medium during cultivation process and reveals the relationship between these microorganisms and the growth of Agaricus bisporus. The domestically grown strains of Agaricus bisporus displayed a higher inhibition growth rate against microorganisms isolated from straw, chicken manure, and medium than imported strains. As for inhibition of mycelial growth among mushroom cultivars of the microorganisms separated by each fermentation step from the mushroom medium, the domestic cultivar, 'Saedo,' grew more vigorously among other cultivars. As the fermentation progressed, it was confirmed that inhibitation of microorganisms against Agaricus bisporus was weakened. A total of 21 strains of microorganisms that promote mushroom growth were isolated in the 4th turning process, and the microorganisms isolated from the mushroom medium affect the growth and as yield of the mushroom through secretory substances.

Evaluation of the Parameters of Soil Potassium Supplying Power for Predicting Yield Response, K2O Uptake and Optiumum K2O Application Levels in Paddy Soils (수도(水稻)의 가리시비반응(加里施肥反応)과 시비량추정(施肥量推定)을 위한 가리공급력(加里供給力) 측정방법(測定方法) 평가(評価) -I. Q/I 관계(関係)에 의(依)한 가리(加里) 공급력측정(供給力測定)과 시비반응(施肥反応))

  • Park, Yang-Ho;An, Soo-Bong;Park, Chon-Suh
    • Korean Journal of Soil Science and Fertilizer
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    • v.16 no.1
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    • pp.42-49
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    • 1983
  • In order to find out the possibility of predicting fertilizer K requirement from the K supplying capacity of soil, the relative K activity ratio, Kas/kai, the potential buffering capacity of $K^+$ ($PBC^k$ ; the liner regression coefficient) and its activity ratio ($AR^k_o$ ; $^{k+}$/${\sqrt{Ca^{+2}+Mg^{+2}}}$ in mol/l) at ${\delta}K$ = O, in the Q/I relationships of Beckett(1964), were determined for the soils before flooding and the samples taken at heading stage of transplanted rice in pot experiment. These parameters assumed as the K supplying capacity of soils were subjected for the investigation through correlation stady between themselves and other factors such as grain yield or the amounts of $K_2O$ uptake by rice plant at harvest. The results may be summarized as follows; 1. The potassium supplying power of the flooded soil was considered to be ruled by the amounts of exchangeable K before flooding, since there was little change in exchangeable K concentration from no-exchangeable K during the incubation periods of 67 days. 2. The $PBC^k$ values, in soils before flooding were 0.027, 0.014 and 0.009, where as the $AR^k_o{\times}10^{-3}$ values were 9.1, 7.6, and 15.4, respectively, in clay, loamy and sandy loam soils. 3. The $PBC^k$ values, determined in the soil samples taken at heading stage, varied little compared with the values of orignal soil, regardless of those different fertilizer treatments and textures, showing the possibility of using them as a factor for the improvement of soil to increase the efficiency of fertilizer K. 4. The significant yield responses to potassium fertilizer application were observed wherever the $AR^k_o$ values in soil at heading stage drop down to the original $AR^k_o$ values, regardless of any levels of fertilizer application. 5. The higher correlations between the gain yield or the amounts of $K_2O$ uptake and by the use of both soil factors of $PBC^k$ and $AR^k_o$ at heading stage were observed compared with the use of any single factor. 6. The Kas/Kai value in the soil, estimated prior to the experiment, had high possitive correlation with the $AR^k_o$ determined in the soil at heading stage and could be used as a soil factor for predicting potassium fertilizer requirement.

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