• YAKHAK HOEJI
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• v.31 no.3
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• pp.154-163
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• 1987

Daidzein and its methyl derivatives were synthesized and their anti-inflammatory activities were examined. Daidzein suppressed the carrageenin-induced edema, but its methyl derivatives showed decrease or abolition of the anti-inflammatory effect. Daidzein did not significantly suppress the complete Freund's adjuvant-induced arthritis, and the mixed phlogistics (histamine+serotonin)-induced edema. Daidzein inhibited the leukocyte emigration and protein exudation when it was administered into the CMC pouch at low doses (5,25mg/pouch). Daidzein significantly suppressed the cotton-pellet granuloma formation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.23 no.1
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• pp.116-119
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• 1994

The anti-inflammatory and analgesic actions of Cedrela sinensis, Oenanthe javanica and ARtemisia princeps var. orientalis were carried out. The methanolic extract of Artemisia princeps var. orientalis showed the inhibitory effect on carrageenin-induced edema as well as vascular permeability in mice. And we also found the alagesic activity in these plants.

• Korean Journal of Clinical Pharmacy
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• v.2 no.1
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• pp.71-73
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• 1992

• Journal of the Korean Applied Science and Technology
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• v.40 no.6
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• pp.1373-1380
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• 2023

This study investigated the effects of coumarin, a flavonoid known for various physiological activities like antiviral, anticancer, and antibacterial properties, on anti-oxidants and anti-inflammatory processes, aiming to explore its application in functional cosmetics. The results are as follows: Cell toxicity experiments using RAW 264.7 cells showed no significant cytotoxicity for coumarin at any concentration, indicating its safety for skin application. Observing coumarin's antioxidant activity through DPPH radical scavenging showed concentration-dependent effectiveness, though not significantly varied with concentration. The inhibition of silica-induced ROS production in cells was concentration-dependent. Both NO production inhibition and histamine release measurements showed concentration-dependent suppression. These findings suggest that Coumarin can be effectively used as a natural ingredient in cosmetic development for its antioxidant and anti-inflammatory properties.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1995.04a
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• pp.56-56
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• 1995

자연계에 널리 분포하고 있는 flavonoid 유도체들은 다양한 구조에 따라, 다양한 생물활성을 보인다. 최근에는 이들이 cyclooxygenase/lipoxygenase를 저해한다는 사실이 알려진 후에 이들 유도체들을 항염증제 또는 항알레르기 약물로 개발하려는 시도가 계속되고 있다. 이들 연구의 일환으로 본 연구자들은 여러 flavonoid유도체들에 대한 in vivo 항염증능 및 림프구증식억제능을 보고하였다 (Arch. Pharm. Res. 16, 18, 25 (1993), 17, 31, 236 (1994), Life Sci. 54, 313 (1994)). 또한 flavonoid 유도체들 중 apigenin-dimer인 수종의 biflavonoid들이 group II phospholipase $A_2$ 저해제인 것을 밝혔고 (BBRC 205, 843 (1994)), 이들 중 몇 종은 림프구증식능에 대하여 억제작용을 보인다는 것을 알았다 (Life Sci. in revision). 본 연구에서는 이들 biflavonoid 유도체들 중 amentoflavone과 ginkgetin을 중심으로 이들의 림프구증식억제능 및 in vivo에서의 항염증작용을 연구하였다. 그 결과, ginkgetin (1-10 $\mu$M)은 Con A와 LPS에 의해 유도되는 림프구중식을 비가역적으로 억제하였으며, 이들 유도체들은 마우스 귀부종에 대하여 복강내투여에서 강력한 항염증능을 보인다는 것을 알았다.

• Korean Journal of Medicinal Crop Science
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• v.12 no.4
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• pp.289-294
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• 2004

Reactive oxygen species (ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. In this study, we have conducted to investigate the antioxidant and hyaluronidase inhibitory activities of Prunus persica Batsch var. davidiana Maximowicz in order to screen the bioactive substances which can be developed as possible anti-inflammatory agents. Among the extracts of Prunus persica Batsch var. davidiana Maximowicz, EtOAc extract exhibited the strongest effect on antioxidant experiment such as DPPH, Ferric- Thiocyanate and Rancimat. Also, EtOAc extract showed a potent hyaluronidase inhibitory activity.

• Journal of the Korea Convergence Society
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• v.13 no.1
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• pp.101-107
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• 2022

Tertomotide is a peptide vaccine developed for anti-cancer therapy. Since it has been found to ameliorate inflammatory symptoms in animal studies and clinical test, we investigated anti-inflammation activity of the tertomotide and the mechanism of action in monocyte in order to assess if tertomotide may serve as an anti-inflammatory agent by checking inflammatory cytokines and related signaling pathway following tertomotide treatment. We found that tertomotide reduced the level of pro-inflammatory cytokines such as TNF-α, IL-1β, IL-8 in LPS- or PMA-stimulated monocyte cell line and suppressed NF-κB signaling including the activation of ERK1/2 and P38 MAPK following TNF-α treatment. These results may correlate to the beneficial findings in animal studies, implicating that tertomotide may act as a potential anti-inflammatory agent. This study is an exemplary case for convergence that a computationally designed peptide for immunological purpose exerting unexpected biological activity may elicit novel anti-inflammatory drug.

• YAKHAK HOEJI
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• v.34 no.6
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• pp.407-414
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• 1990

The relationships of inhibitory activities of inflammation and wound healing of flavonoids were studied in vitro and in vivo. Generally flavonoids have not only significantly anti-inflammatory activity in carrageenin-induced paw edema and Freund's complete adjuvant-induced arthritis, but also inhibitory activity of wound healing. The more inhibitory activities of wound healing flavonoids have, the more they have the anti-inflammatory activities; apigenin > guercetin > flovone > rutin > hesperidin > naringin. Their inhibitory mechanism seems to be inhibition of the inflammatory cell infiltration and fibroblast proliferation, and so they decreased the granulomatous activity and tensile strength.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.20 no.1
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• pp.25-36
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• 1994

It is weal known that bacterial lipopolysaccharide (LPS) stimulates prostaglandin synthesis in various experimental system via enhancing the expression of cylooxygenase-2 (COX-2). This study was designed to characterize U)5-induced prostaglandin synthesis in mouse peritoneal macrophages LPS-stimulated prostaglandin synthesis in macrophages with short term exposure was not so much prominent, but there was a burst in prostaglandin synthesis 8 hours after the LPS treatment and this u·as accompanied with the increase of cyclooxygenase activity, Dexamethasone markedly inhibited prostaglandin synthesis in this system. Metabolic label ins data supported above observations and thus, it could be concluded that LPS induces the do novo synthesis of COX-2 by which it stimulates the prostaglandin synthesis in mouse peritoneal macrophages, These data suggested that this experimental model system could be used for the screening procedure of COX-2 selective inhibitors. Ketoprofen, a non steroidal anti inflammatory agent, appeared to inhibit COX-1 relatively more selectively than COX-2.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.84-84
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• 2020

염증 (Inflammation)은 병원체, 손상된 세포, 자극물질 등으로 인한 손상에 대해 작용하여 조직이나 장기의 손상을 재생하는 작용으로써 신체 방어 기전들 가운데 매우 중요한 역할을 하는 것으로 알려져 있다. 이러한 염증반응이 과다할 경우 각종 염증성 질환 혹은 암 등을 유발하는 원인으로 발전할 수 있어 항염증제의 개발은 전 세계적으로 중요시되고 있다. 선천적 면역을 담당하는 대식세포는 lipopolysaccharide(LPS), 활성산소 (ROS) 등에 의해 자극되어 염증인자 생성에 관여한다. 따라서 본 연구에서는 항염증 소재 개발을 목표로 하며, 국내 천연소재를 활용한 기능성 항염증 소재로 전북 순창군에서 재배된 초석잠(Stachys Sieboldii MIQ.)과 돼지감자 (Helianthus tuberosus L.)를 이용하여 항산화 활성을 평가하고자 하였으며, 대식세포주를 활용한 세포독성 및 항염증 활성에 대한 효능을 확인하고자 하였다. 본 연구는, 전북 순창 지역에서 재배 된 초석잠과 돼지감자를 사용하여 각 조건의 추출 용매, 온도, 시간별 추출물의 Total polyphenol 함량 평가를 통한 최적 추출조건 선정을 진행하고, 선정된 추출조건의 추출물의 항산화 활성을 측정하기 위해 DPPH & ABTS radical scavenging activity 분석 및 Total flavonoids 함량 분석을 통해 항산화 효능 평가를 진행하였다. 또한 항염증 소재로의 활용을 위해 대식세포인 Raw 264.7을 사용하여 농도별 MTT assay를 진행하여 세포독성 평가를 진행하였고, Nitric oxide (NO) 생성억제 효능을 확인하여 항염증 활성을 평가하였다. 실험 결과, Total polyphenol 함량 분석을 통해 최적 추출조건이 선정된 초석잠 (25℃, 주정 60%, 3 h), 돼지감자 (25℃, 주정 40%, 1 h)은 최적 조건에서 약 58 mg GAE/g 및 158 mg GAE/g의 총 폴리페놀 함량을 보였으며, DPPH & ABTS radical scavenging activity 및 Total flavonoids 함량 분석한 결과, 초석잠이 돼지감자보다 더 높은 항산화 활성을 나타내었다. 대식세포 실험에서의 추출물 처리군의 세포독성 측정 결과, 100 ug/mL 이내의 농도에서 독성활성이 나타나지 않음을 확인하였고, Nitric oxide (NO) 생성 억제활성 측정을 통해 LPS 처리군 대비 접종량 100 ug/mL 기준 각각 초석잠 약 47%, 돼지감자 약 49% 수준의 항염증 활성을 확인하였다.