• Korean Journal of Food Science and Technology
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• v.33 no.4
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• pp.401-408
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• 2001

The ethanol extract and n-hexane fraction of Commiphora molmol Engl. showed minimum inhibitory concentration of 50 ppm and 25 ppm, respectively, on 5 strains of Listeria monocytogenes at $32^{\circ}C$. The purified substance, C3-3-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Commiphora molmol Engl. The C3-3-2 fraction showed a strong bactericidal activity on 5 strains of L. monocytogenes at the concentration of 10 ppm in tryptic soy broth medium. At that concentration, the viable count was reduced $5{\sim}6$ log cycle from initial cell number. The n-hexane fraction of Commiphora molmol Engl. showed strong growth inhibition at the concentration of 25 ppm on Bacillus cereus and Staphylococcus aureus, at 50 ppm in broth on Salmonella enteritidis, and at 500 ppm on Vibrio parahaemolyticus. The purified antimicrobial substance, the C3-3-2 fraction, was identified as m-nonylphenol by on the basis of the $^1H-,\;^{13}C-NMR$ and EI/MS data. For the application test, the C3-3-2 fraction which was purely isolated from Commiphora molmol Engl. at 100 ppm were applied to minced Alaska pollack and ground beef at $32^{\circ}C$ and $5^{\circ}C$. The antimicrobial substances did not reduce L. monocytogenes ATCC 19113 at $32^{\circ}C$, while they reduced L. monocytogenes ATCC 19113 in viable number at $5^{\circ}C$. However, the antimicrobial effect of C3-3-2 fraction in food system was lower than that of broth condition.

• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.274-282
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• 2002

The ethanol extract and n-hexane fraction from Hypericum ascyron L. showed strong growth inhibition at 25 ppm on 5 strains of Listeria monocytogenes for 72 hr at $32^{\circ}C$. The purified substance, H2-5-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Hypericum ascyron L. The H2-5-2 fraction showed a strong bacteriostatic activity on 5 strains of L. monocytogenes at 10 ppm in tryptic soy broth, and the viable cell was reduced 1 log cycle compared to initial cell number. The n-hexane fraction of Hypericum ascyron L. showed strong growth inhibition at 25 ppm on Bacillus cereus and Staphylococcus aureus, and at 50 ppm on Vibrio parahaemolyticus for 72 hr. The purified antimicrobial substance, the H2-5-2 fraction, was assumed as high unsaturated sterol by $^1H-NMR$ and $^{13}C-NMR$. On application test using minced Alaska pollack and ground beef, the n-hexane fraction of Hypericum ascyron L. at the level of 250 ppm was applied at $32^{\circ}C$ and $5^{\circ}C$. At $32^{\circ}C$ storage condition, the antimicrobial substances did not reduced L. monocytogenes ATCC 19113, meanwhile at $5^{\circ}C$ storage condition, L. monocytogenes ATCC 19113 was reduced in viable number.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2002.05a
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• pp.110-115
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• 2002

• The Magazine of the Society of Air-Conditioning and Refrigerating Engineers of Korea
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• v.29 no.10
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• pp.28-34
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• 2000

차량용 에어컨의 증발기 표면에 공기가 지나갈 때 결로로 인한 통풍저항 증대와 비산 현상을 억제하며 세균번식과 냄새가 발생하는 증발기에 항균성과 탈취효과를 주기 위해, 친수-항균재가 세계적으로 1980년대부터 적용되어 왔다. 본 고에서는 친수-항균재의 기술개발 동향과 평가기술을 소개하고자 한다.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.261-265
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• 1994

To detect naturally occurring bioactive compounds in unused marine resources such as marine by-products and algae, the screening test for the antimicrobial compounds containing in Asterina pectinifera, Halocynthia rotetzi skin, Nototodarus sloani ink. Anthocidaris crassispina skin, and four algae(Sargassum horneri, Agarum cribrosum, Odonthalia corymbifera and Desmarestia ligulata) was carried out. The minimum inhibitory concentrations(MIC, ${\mu}g/disk$) of ether and methanol fractions extracted from Sargassum horneri against Bacillus subtilis were 80 and 70 ${\mu}g/disk$, respectively. The MICs of the ether, acetone and methanol fractions extracted from Sargassum horneri against Staphylococcus aureus were 160, 180 and 70 ${\mu}g/disk$, respectively. In addition, the MICs of ether and acetone fractions extracted from Odonthalia corymbifera against Staphylococcus aureus were 50 and 170 ${\mu}g/disk$, respectively. These nonpolar fractions showed the strongest antimicrobial effect among several fractions extracted from tested marine resources.

• Proceedings of the KAIS Fall Conference
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• 2000.04a
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• pp.22-22
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• 2000

• Research in Plant Disease
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• v.21 no.1
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• pp.12-19
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• 2015

Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve different nano-materials were used to observe the antagonistic activity against three kinds of mold and sixteen different kinds of watermelon fruit rot pathogens (Acidovorax citrulli). According to the results, no antagonism have been found against the pathogen, Cylindrocarpon destructans. However in the case of Pythium ultimum, combination of Brass/Glucose 1,000 ppm confirmed the mycelial growth reduction by 94%. In addition, little effect was found against Rhizoctonia solani by Ag/Glucose 3,000 ppm. The remaining other nano-materials have different antimicrobial effect depending on the strains of A. citrulli. But in the case of lime (Cu/Salt 1,000 ppm) highest antimicrobial activity was observed with 97%. Moreover growth of five different strains of A. citrulli was checked by 99% with the combination of Ag/Glucose 1,000 ppm. 92% reduction of A. citrulli growth was observed with $Brass/CaCO_3$ 3,000 ppm. Tested nano-materials against different plant pathogens in this study showed the antimicrobial activity at the range of 24-70%.

• Food Science and Preservation
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• v.14 no.3
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• pp.323-327
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• 2007

Moderate consumption of maesil(Prunus mune) was associated with pharmaceutical and physiological effects on human health. The object of this study was to determine the inhibitory effects of Prunus mune extracts(PME) on food spoilage microorganisms. PME was found to have an antibacterial effect on Colletotrichum fragariae. The hydrophilic fractions of PME showed more effective inhibition than did the hydrophobic fractions. In addition, the hydrophilic fractions of PME seemed to inhibit(12-40%) metabolic enzymes related to energy production, including glucose-6-phosphate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, and hexokinase. Our data suggest that hydrophilic components of PME might control food spoilage microorganisms because of suppression of membrane enzymatic function.

• Journal of Food Hygiene and Safety
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• v.15 no.4
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• pp.277-281
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• 2000

Agar, one of the most abundant marine products has not been utilized extensively because of low level of processing technology in Korea. This research was carried out to improve the utilization of agar and consequent increase in profit. Antibacterial activity of agarooligosaccharides were evaluated against bacteria causing putrefaction and flood poisoning. Addition of 0.4% agarooligosaccharides showed antibacterial activity toward Staphylococcus aureus and Escherichia coli O157:H7; furthermore, autoclave treatment of agarooligosaccharides solution enhanced the antibacterial activity. Agarooligosaccharides showed high stability against the pH change. Addition of amino acid(alanine, lysine, glycine, phenylalanine) in agarooligosaccharides solution enhanced antibacterial activity in E. coli O157:H7, Streptococcus mutans and Staphylococcus aureus.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.3 s.31
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• pp.68-74
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• 2006

목적 : 삼나무(Cryptomeria japonica)로부터 얻은 정유의 구성과 구강박테리아에 대한 항균작용을 연구하였다. 방법 : 정유의 화학적조성은 GC 와 GC-MS에 의하여 분석하였고, 정유와 그 주성분에 대한 구강박테리아 15균주의 항균효과를 시험하였다. 결과 : 정유의 95.82%에 해당하는 68복합체를 증명할 수 있었다. 정유의 주요성분은 ${\alpha}-pinene$ (6.07%), sabinene (8.86%), limonene (3.43%), ${\gamma}-cadinol$ (4.28%), ${\beta}-eudesmol$ (3.90%), and 10(15)-cadien-4-ol (7.16%)이었다. 삼나무의 주요성분은 거의 모든 박테리아에 대하여 MICs, 0.025 to 0.05 mg/ml; MBCs, 0.025 to 0.1 mg/ml에서 유의성있는 억제효과를 나타내었고, 주요성분 또한 다양한 성장억제를 나타내었다. 결론 : 삼나무의 정유는 다종의 화학복합체로 이루어져 있으며, 이는 다종의 구강박테리아에 대해 항균작용을 갖고 있다.