• Journal of Ginseng Research
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• v.12 no.2
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• pp.104-113
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• 1988

Effect of the purified ginsenoside $-Rb_1$ and $-Rb_2$ on LDL receptor biosynthesis of CHO cell cultured in a high cholesterol medium was investigated . Cholesterol uptake by CHO cell cultured in a medium containing various amounts of cholesterol was traced and found that the cholesterol uptake was proportional to the concentration of cholesterol in the medium, and the population of LDL receptors were proportionally decreased as the increasing cholesterol level in the cell. However, when the CHO cells were cultured in the medium containing ginsenosides, no significant decrease of LDL receptor population occured. The biosynthesis of protein and RNA of the above cells was higher than that of CHO cells cultured in the absence of the ginsenosides, suggesting that the ginsenosides might stimulate LDL receptor bio-synthesis. It was also observed that the ginsenosides stimulated the biosynthesis of estradiol and progesterone from cholesterol in the CHO cell. From the above results, it seemed that the ginsenosides lowers the cholesterol level by stimulating the cholesterol metablism including steroid hormone biosynthesis, resulting in the lowering of inhibitory action of cholesterol on LDL receptor biosynthesis.

• Journal of Life Science
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• v.33 no.10
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• pp.842-850
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• 2023

Pyrrolnitrin, pyrrolomycin, and pyoluteorin are functional halogenated phenylpyrrole derivatives (HPDs) derived from microorganisms with diverse antimicrobial activities. Pyrrolnitrin is a secondary metabolite produced from L-tryptophan through four-step reactions in Pseudomonas fluorescens, Burkholderia cepacia, Serratia plymuthica, etc. It is currently used for the treatment of superficial dermatophytic fungal infections, has high antagonistic activities against soil-borne and foliar fungal infections, and has many industrial applications. Since pyrrolnitrin is easily decomposed by light, it is difficult to widely use it outdoors. As an alternative, fludioxonil, a synthetically produced non-systemic surface fungicide that is structurally similar and has excellent light stability, has been commercialized for seed and foliar treatment of plants. However, due to its high toxicity to aquatic organisms and adverse effects in human cell lines, many countries have established maximum residue levels and strictly control its levels. Pyrrolomycin and pyoluteorin, which have antibiotic/antibiofilm activity against Gram-positive bacteria and high anti-oomycete activity against the plant pathogen Pythium ultimum, respectively, were isolated and identified from microorganisms. This review summarizes the biosynthesis and production of natural pyrrolnitrin derived from bacteria and the characteristics of synthetic fludioxonil and other natural phenylpyrrole derivatives among the HPDs. We expect that a plethora of highly effective, novel HPDs that are safe for humans and environments will be developed through the generation of an HPD library by microbial biosynthesis and chemical synthesis.

• Journal of Korean Society of Steel Construction
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• v.17 no.1 s.74
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• pp.63-71
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• 2005

There were already several studies conducted on the steel-concrete (SC) composite column, which was developedcomplement the weaknesses and maintain the advantages of previous composite columns. The axial compressive capacity of the SC composite column was estimated by the tests in previous studies, but the experiments for the large-scale column could not be performed because of the limitation with the laboratory's capacity. In this study, the analytical study was performed using the general finite element analysis program to reflect the interaction of concrete and steel and the local buckling of steel flange composed of the non-compact section. The appropriateness of the analytical model was verified by the comparison between experimental and analytical results. The nonlinear behavior of full-scale SC composite column was analyzed using the verified analytical model. From these analytical studies, it was concluded that the width-to-thickness ratio of the steel cross-section of the SC composite column should not exceed 25:0. The section area of the link is best when it is over 0.025 dt, and the link distance is to be less than D/2 or 300mm.

• Journal of the Korea Concrete Institute
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• v.23 no.3
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• pp.339-346
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• 2011

The effects of mineral admixtures on the bonding properties of cement mortar to polyolefin based synthetic fiber were evaluated. The mineral admixtures consisted of 0%, 5%, 10%, and 15% fly ash, blast furnace slag, and metakaolin in cement. Bond interactions between the cement mortar and the polyolefin based synthetic fiber were determined by Dog-bone bond tests. Bond tests of the polyolefin based synthetic fiber showed an increase in pullout load with the strength of the cement mortar. Also, the interface toughness of polyolefin based synthetic fiber in cement mortar increased as the fly ash, blast furnace slag, and metakaolin contents increased. The microstructure of polyolefin based synthetic fiber surface was examined after the pullout test to analyze the frictional resistant force according to the replacement ratio of fly ash, blast furnace slag, and metakaolin during the pullout process of polyolefin based synthetic fiber in cement mortar. The scratched of polyolefin based synthetic fibers increased with the replacement ratio of fly ash, blast furnace slag, and metakaolin. Also, the interface toughness was enhanced by adhesion forces induced by the fly ash, blast furnace slag, and metakaolin.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.12 no.4
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• pp.525-535
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• 1999

In this study, a new method of fabricated concrete deck bridge construction is proposed. This paper details the method in which concrete multi-girders and fabricated concrete decks are rested on the upper flange of the girder and the female to female type sheat-key is formed to connect girder and deck. The finite element analysis is performed to verify the accuracy of the structural behaviors of the fabricated concrete deck bridge by comparing with experimental results. The first task performed is the analysis of the equilibrium of the member force occurring between the deck and the girder. After verifying equilibrium of the member force determined by the finite element analysis, this process is applied to the analysis of maximum member force as the position of design load. This task is utilized to determine the safety of each member according to the same scale finite element model. The final process in this study is to compare the deflection of girders used in experiment with that of the same scale finite element model to verify the strength of fabricated cincrete deck bridge. By this comparison, it is shown that the behavior of the fabricated concrete deck bridge is almost same as the finite element analysis. The second task is to analyze the load distribution effect according to the number of diaphragms and the composite effect due to the cinnection of the deck and girder by the finite element analysis. From the results of second task, it is found that the load distribution effect is not related to the number of diaphragms in case of the central loading, but is related to the number of diaphragms for eccentric loading. Analysis of the load distribution indicates that the effective number of diaphragm is three. It is also shown that the maximum deflection is decreased to almost one half due to the composite action of the deck and girder.

• Applied Chemistry for Engineering
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• v.34 no.4
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• pp.434-443
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• 2023

We have investigated the performance improvement of grease by synthesizing calcium sulfonate grease as an alternative to lithium grease, which is widely used globally. Since the composition ratio of the grease, when manufactured, is usually 50% base oil and 50% thickener, using grease as a lubricant in a cryogenic environment is not encouraged due to its inferior low-temperature performance. In this study, we have synthesized three types of calcium sulfonate grease with paraffin oil and PAO-based base oil and thickener. Furthermore, lithium grease was synthesized via saponification with PAO-based base oil, lithium hydroxide, 12-hydroxystearic acid, and sebacic acid. We have measured low-temperature characteristics using a rheometer and low-temperature torque meter, and tribology characteristics were obtained using a four-ball lubricant tester and schwingung reibung verschleiß (SRV). As a result, the flow point of the calcium sulfonate grease synthesized with a PAO-based base oil and thickener was found to be -40℃, overcoming the existing calcium sulfonate grease's low-temperature limitation. Moreover, the synthesized calcium sulfonate grease showed low-temperature performance similar to that of lithium grease synthesized with PAO base oil, but superior anti-wear, extreme pressure, coefficient of friction, heat resistance, adhesion, and corrosion resistance. It is thus expected to commercially replace the existing lithium grease.

• Journal of Life Science
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• v.6 no.3
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• pp.185-192
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• 1996

Bacteriophage T7 gene 2.5 protein, a single-stranded DNA binding protein, is required for T7 DNA replication, recombination, and repair. T7 gene 2.5 protein has two distinctive domains, DNA binding and C-terminal domain, directly involved in protein-protein interaction. Gene 2.5 protein participates in the DNA replication of Bacteriophage T7, which makes this protein essential for the T7 growth and DNA replication. What gene 2.5 protein makes important at T7 growth and DNA replication is its binding affinity to single-stranded DNA and the protein-protein important at T7 DNA replication proteins which are essential for the T7 DNA synthesis. We have constructed pGST2.5(WT) encoding the wild-type gene 2.5 protein and pGST2.5$\Delta $21C lacking C-terminal 21 amino acid residues. The purified GST-fusion proteins, GST2.5(WT) and GST2.5(WT)$\Delta$21C, were used for whether the carboxyl-terminal domain participates in the protein-protein interactions or not. GST2.5(WT) and GST2.5$\Delta$21C showed the difference in the protein-protein interaction. GST2.5(WT) interacted with T7 DNA polymerase and gene 4 protein, but GST2.5$\Delta$21C did not interact with either protein. Secondly, GST2.5(WT) interacts with gene 4 proteins (helicase/primase) but not GST2.5$\Delta$21C. these results proved the involvement of the carboxyl-terminal domain of gene 2.5 protein in the protein-protein interaction. We clearly conclude that carboxy-terminal domain of gene 2.5 protein is firmly involved in protein-protein interactions in T7 replication proteins.

• KSBB Journal
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• v.8 no.5
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• pp.424-430
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• 1993

The anti-stress effect by the treatment of vitamin C was investigated in this study. The treatment of ascorbic acid in the presence of $Cu^{2+}$ion induced strong time- and dose-dependent degradation of hitamine, and also the addition of histamine accelerated time-dependent decomposition of ascorbic acid in vitro. The treatment of ascorbic acid in $ODS^{od}/_{od}$rats, which cannot synthesize ascorbic acid, significantly decreased the urinary histamine. The protreatment of ascorbic acid, dexamethasone and promethazine inhibited the lethal effect induced by immobilization stress, but that of dimethylsulfoxide did not. The addition of ascorbic and to a culture of spleen cells of $ODS^{od}/_{od}$rats significantly increased the Con A-dependent T lymphocyte proliferation.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.37 no.12
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• pp.1559-1565
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• 2013

This study aims to investigate the dynamic interaction characteristics between Maglev vehicles and an elevated guideway. A more detailed model for the dynamic interaction of the vehicle/guideway is proposed. The proposed model incorporates a 3D full vehicle model based on prototyping, flexible guideway by a modal superposition method, and levitation electromagnets including the feedback controller into an integrated model. The proposed model was applied to an urban transit Maglev developed for a commercial application to analyze the dynamic response of the vehicle and guideway, and the effect of the surface roughness of the rail, mid-span guideway deflections, and air gap variations are then investigated from the numerical simulation.

• Applied Chemistry for Engineering
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• v.25 no.5
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• pp.548-551
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• 2014

In order to reduce toxicity on the human body, four new cleaning agents (1-4) containing ester and ether functionalities have been invented. The synthesized cleaning agents's physical properties, biodegradabilities, and $LD_{50}$ values, which were conducted by Korea Testing Certification Institute by using standard method, showed excellent values. A specimen for cleaning ability was prepared by cutting in $60{\times}40mm$ size of stainless steel plate. The surface of the above specimens was treated with four different kinds of contaminants, such as cutting oil, anti-rust oil, drawing oil, and lubricating oil. Contaminated specimens were then immersed in compounds (1-4) for 1 to 5 minutes to dissolve oil in the cleaning agent. The data indicate that all compounds (1-4) exhibit good cleaning ability toward four contaminant oils. It is also confirmed that these compounds can be applicable to various industrial cleaning fields as nontoxic and biodegradable cleaning agents because of their excellent biodegradabilities and $LD_{50}$ values.