• 대한의료기공학회지
• /
• 제4권1호
• /
• pp.179-200
• /
• 2000

• 대한의료기공학회지
• /
• 제4권2호
• /
• pp.228-241
• /
• 2000

• 대한의료기공학회지
• /
• 제8권1호
• /
• pp.82-95
• /
• 2005

• 한국환경과학회지
• /
• 제28권11호
• /
• pp.993-1004
• /
• 2019

Chrysanthemum boreale Makino (C. boreale) is widely distributed in Asian countries, and has traditionally been used to treat various inflammatory diseases including bronchitis. In this study, we aimed to isolate biologically active compounds from leaves and stems of C. boreale. Chemical components were purified by column chromatograpy and recyclic HPLC, and characterized from their spectral data (IR, MS, NMR). Biological activity experiments were conducted for Farnesyl-protein transferase (FPTase) activity, apoptosis and nitirc oxide (NO) release. As a results, three sesquiterpene lactones were isolated. Compound 1 (4-methoxy-8-O-acetyl-10-hydroxy-2,11(13)-guaiadiene-12,6-olide) showed strong cytotoxic activities having an average growth inhibition of 50% ($GI_{50}$) value of $1.89{\mu}g/m{\ell}$ against human colon adenocarcinoma cells. Compound 1 also showed a low half maximal inhibitory concentration ($IC_{50}$) value of $10{\mu}g/m{\ell}$ for NO release. In the caspase 3 activity, compound 1 and compound 2 (8-O-(2-carbonyl-2-butyl)-3,10-dihydroxy-4,11(13) -guaiadiene-12,6-olide) exhibited 94% and 90% apoptosis inhibition activity, respectively. Compound 3 (4,8-O-diacetyl -10-hydroxy-2(3),11(13)-guaiadiene-12,6-olide) showed a strong inhibitory effect on FPTase activity with 90% inhibitory activity at a concentration of $100{\mu}g/m{\ell}$. These results clearly show the presence of lactone compounds in the leaves and stems, which may partially contribute to the pharmacological activity of C. boreale.

• 동의생리병리학회지
• /
• 제32권6호
• /
• pp.355-360
• /
• 2018

The purpose of this study is to find out the direction of development of the expert diagnosis system by questioning student satisfaction after practicing Oriental Diagnosis System (ODS). A questionnaire survey was conducted for 73 students who attended Pusan National University Graduate School of Korean Medicine and conducted clinical practice from the second semester of 17 years to the first semester of 18 years. The questionnaire survey consisted of 6 questions about the satisfaction of current expert system and 5 questions about supplementation and addition. As a result of the questionnaire analysis, the question about the necessity of the expert diagnosis system and the benefit of clinical practice using the expert diagnosis system indicated high positive response rate. On the other hand, there is a difference in the results of the dialysis before and after using the expert diagnosis system. So, It seems that the reliability of the results of the expert diagnosis system is still low. The results of the questionnaire indicate that the need for expert diagnosis system is beneficial, but it is necessary to supplement the current system in order to improve clinical utility in the future.

• 대한한의학방제학회지
• /
• 제27권1호
• /
• pp.45-52
• /
• 2019

Objectives : Hyperthermia is a widely used therapeutic tool for cancer therapy and a well-known inducer of apoptosis. Although the Cinnamomi cortex (CC) is a potent anticancer agent for several human carcinomas, it is less potent in the human U937 cell line. To explore any enhancing effects of CC with hyperthermia induced apoptosis, this study investigated the combined effects and apoptotic mechanisms of hyperthermia and CC in U937 cells. Methods : U937 cells were heat treated at $43^{\circ}C$ for 30 min with or without pre-treatment for 1h with CC and then incubated at $37^{\circ}C$ with 5% $CO_2$. Cell viability was analyzed by MTT assay and Trypan blue assay. Morphological changes reflecting apoptosis were visualized under microscope. Synergy effect of CC combined with hyperthermia were calculated by Compusyn software. The expression of proteins related to apoptosis and signaling pathways was determined by western blotting. Results : Hyperthermia with CC reduced cell viability and induced apoptosis. Combined hyperthermia and CC treatment markedly augmented apoptosis by upregulating proapoptotic proteins and suppressing antiapoptotic proteins, culminating in caspase-3 activation. Furthermore, the combined treatment, decreased the expression of in Bcl-2 family, cyclin D1, VEGF, MMP2 and MMP9 expression. Conclusion : This study provides compelling evidence that hyperthermia, in combination with CC, is a promising therapeutic strategy for enhancement of apoptosis and suggests a promising therapeutic approach for cancer.

• 한국환경과학회지
• /
• 제32권1호
• /
• pp.67-76
• /
• 2023

Under constant environmental pollution, the incidence of Atopic Dermatitis (AD) caused by air pollutants and allergens has increased. AD is an allergy inflammatory skin disease characterized by pruritus, eczema, and skin dryness. In herbal medicine, Anemarrhena asphodeloides (Anemarrhenae Rhizoma; AR) has been utilized to treat Alzheimer's disease, osteoporosis, hypertension, and inflammation. The purpose of study evaluated the effect of AR in a mouse model of 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions. After acclimatization for 5 days, the mice (6-week-old, male Balb/c) were divided into five groups (n=6/group): NC (normal control), DNCB (control), Dex (5 mg·kg-1, p.o.), AR100 (100 mg·kg-1, p.o.), and AR300 (300 mg·kg-1, p.o.). On days 1 and 3, 1% DNCB was applied to the skin and ears. After 4 days, 0.5% DNCB was applied once every 2 days for 2 weeks. Then, skin and ears eczema area and severity index (EASI); skin nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) levels; and plasma immunoglobulin E (IgE) levels were examined. The AR groups showed lower EASI, skin and ear thickness, mast cell count, and IgE levels than the control groups. Moreover, AR reduced iNOS, COX-2, and PGE2 levels. Therefore, AR possesses anti-inflammatory properties and can improve skin damage, indicating its therapeutic potential against AD.

• 대한화장품학회지
• /
• 제49권1호
• /
• pp.9-16
• /
• 2023

본 연구에서는 벨벳콩(Mucuna pruriens, M. pruriens) 추출물을 활용하여 천연소재 기반 항산화 기능성 화장품 소재로서의 활용가치를 검증하고자 하였다. 벨벳콩을 물과 70% 에탄올, 70% 아세톤을 용매로 하여 추출, 농축, 동결건조한 다음 각각의 분말화된 벨벳콩 열수 추출물(MW), 벨벳콩 70% ethanol 추출물(ME), 벨벳콩 70% acetone 추출물(MA) 시료를 확보하였다. MW, ME, MA를 활용하여 DPPH 라디칼 소거 활성, ABTS⁺ 라디칼소거 활성, SOD 유사 활성, 환원력, 총 페놀 및 플라보노이드 함량 분석을 통해 항산화 활성을 분석한 결과, 모두 우수한 항산화 효능을 나타내었다. 따라서 벨벳콩 추출물은 항산화 관련 기능성 화장품 천연소재로서의 활용 가능성이 높을 것으로 사료된다.

• 대한본초학회지
• /
• 제33권2호
• /
• pp.1-7
• /
• 2018

Objectives : This study was conducted to evaluate the antioxidant activity and inhibitory activity of pancreatic lipase in vitro using natural products sangju dungsi, chungdo bansi, kabju baekmok three kind of young persimmon fruit extracts and to develop a high - functional anti - obesity substance with reduced body fat. Methods : The antioxidative effect of the three kind of young persimmon fruit extracts was determined by hot water extraction, 30% EtOH extraction and 70% EtOH extraction. The antioxidant activities were evaluated through radical scavenging assays using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals. Furthermore, we examined total phenol and flavonoids contents. And the pancreatic lipase inhibition was measured by selecting the sangju dungsi extract with antioxidant effect. Results : The yield of water, 30% and 70% EtOH extract from young persimmon fruit was sangju dungsi (water 8.3%, 30% EtOH 18.1%, 70% EtOH 16.9%), chungdo bansi (water 16.2%, 30% EtOH 16.1%, 70% EtOH 11.6%), kabju baekmok (water 16%, 30% EtOH 16.8%, 70% EtOH 11.5%). The sangju dungsi 30% EtOH extract eminent DPPH ($IC_{50}$, $19.25{\pm}2.94{\mu}g/m{\ell}$) and ABTS ($IC_{50}$, $19.94{\pm}4.07{\mu}g/m{\ell}$) radical scavenging activity, and total phenol $52.37{\pm}0.78mg/g$ and flavonoid $7.97{\pm}0.13mg/g$ contents were also excellent. It also showed excellent pancreatic lipase inhibition efficacy. Conclusion : The extracts from the three kind of young persimmon fruit extracts showed excellent antioxidant activity. sangju dungsi 30% EtOH extract may be a new anti-obesity material by reducing fat absorption through the inhibition of pancreatic lipase.

• 제3의학
• /
• 제2권1호
• /
• pp.7-12
• /
• 1997

In order to basic study of the oriental medical engineering, the Qigong infrasound treatment apparatus is analyzed. The results is followed : 1. SY-201 type Qigong Infrasound Treatment Apparatus is composed of oscillator, amp, transducer. 2. The Qi production process is analyzed that EM wave changed sound wave of superlow frequency number and penetrated the human body deeply.