• Title/Summary/Keyword: 하천실험

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Development of selective media for Enterococci (장구균 검출 배지 개발)

  • Chang, Dong-Ho;Yoon, Jun-Beom;Lee, Keun Heon;Park, Kyeong Ryang
    • Korean Journal of Microbiology
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    • v.52 no.1
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    • pp.25-31
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    • 2016
  • An enterococci selective medium was developed to detect the presence of enterococci for use as a fecal contamination indicator. Among several media which have been known to detect enterococci, the following 9 different kinds of media were selected: Enterococci Confirmatory agar, Azide dextrose agar, Bromocresol-purple azide agar, Esculin bile agar, Citrate azide tween carbonate agar, KF Streptococcus agar, BROLACIN agar, Kanamycin esculin azide agar, and Membrane filter Enterococcus selective agar. Various components from the nine media were mixed to develop a more effective enterococcus selective medium. The newly developed medium named as 'Enterococcus Mixed medium' was more effective than the previous 9 media. Enterococci strains (Enterococcus avium KACC 10788, Enterococcus faecium KACC 11954, Enterococcus saccharolyticus KACC 10783, Enterococcus durans KACC 10787, Enterococcus faecalis KACC 11304, and Enterococcus hirae KACC 10779) and non-enterococci strains (Escherichia coli KACC 10005, Staphylococcus aureus subsp. aureus KACC 10768, and Bacillus subtilis KACC 10111) were used to test the new medium. As a result, the enterococci strains grew well on the Enterococcus Mixed medium whereas the non-enterococci strains did not grow well on it. Additionally, growth of enterococci with freshwater and seawater samples was observed to be good on the Enterococcus Mixed medium. The result of this study confirmed that the Enterococcus Mixed medium was effective in detecting the target enterococci.

Iron Extraction Characteristics of Sediment Samples from a River Bank Filtration Site (강변 여과 취수 지역 퇴적물의 철 화학종 추출 특성)

  • Hyun, Sung Pil;Moon, Hee Sun;Yoon, Pilsun;Kim, BoA;Ha, Kyoochul
    • Journal of the Mineralogical Society of Korea
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    • v.26 no.2
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    • pp.129-138
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    • 2013
  • River bank filtration has been considered as a promising alternative water management scheme, in which groundwater is extracted from an aquifer near a river after infiltration of the river water into the aquifer, thereby improving and maintaining the quality of water recovered. Iron (Fe) associated with sediment in contact with groundwater and infiltrating surface water is an important factor in determining the quality of water recovered from the pumping wells in river bank filtration. This study reports the results of Fe speciation in the aquifer sediment samples collected from different depths at the river bank filtration site in Changwon, studied using four different chemical extraction methods, namely, ferrozine, oxalate, HCl, and DCB methods. Overall, the results show that Fe(II) as well as the total Fe content decreases with depth down to ~20 m and then increases further below. This trend is consistent with the redox characteristics suggested by visual observation. The silt/clay size fraction (${\phi}$ < 62.5 ${\mu}M$) has up to 2~10 times more Fe compared with the sand size fraction (62.5 ${\mu}M$ < ${\phi}$ < 2 mm), depending on the extraction method. Of the four extraction methods, DCB solution extracted the most Fe from the sediment samples. The amounts of Fe extracted by the different extraction methods can be a good indicator of the redox conditions along the depth of the aquifer.

Analysis of Microbial Community during the Anaerobic Dechlorination of PCE/TCE by DGGE (DGGE를 이용한 PCE 및 TCE의 혐기적 탈염소화 군집의 미생물 군집분석)

  • Kim, Byung-Hyuk;Cho, Dae-Hyun;Sung, Youl-Boong;Ahn, Chi-Youg;Yoon, Byung-Dae;Koh, Sung-Cheol;Oh, Hee-Mock;Kim, Hee-Sik
    • Microbiology and Biotechnology Letters
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    • v.38 no.4
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    • pp.448-454
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    • 2010
  • This study investigated the effect of PCE and TCE as electron acceptors on the bacterial composition of dechlorinating communities. The enrichment cultures reductively dechlorinating PCE and TCE were developed from three environment samples using acetate as electron donor. The cultures were prepared by sequential enrichment, which was seeded with sediment and dredged soil. Denatured gradient gel electrophresis (DGGE) of 16S rRNA gene fragment was used to compare the microbial communities of these three enrichment cultures. After incubation for 4 weeks, the removal efficiencies of PCE and TCE were highest from Yeocheon site (87.37% and 84.46%, respectively). PCE and TCE as electron acceptors affected the bacterial diversity and community profiles in the enrichment cultures. DGGE analysis showed that the dominant bacteria in PCE and TCE enrichment were belonged to Clostridium sp., Desulfotomaculum sp., and uncultured bacteria.

Distribution and Migration Characteristics of Explosive Compounds in Soil at Military Shooting Ranges in Gyeonggi Province (경기도 북부지역 군용 사격장 토양에 존재하는 화약물질 분포 및 이동 특성 조사)

  • Bae, Bumhan;Park, Jieun
    • Journal of the Korean GEO-environmental Society
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    • v.15 no.6
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    • pp.17-29
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    • 2014
  • A remedial investigation was conducted at five military training ranges in northern Gyeonggi province to collect information necessary for the design of on-site treatment facilities for the abatement of explosive compounds release to the environment. These information includes (i) identification of dominant explosive compounds in each range, (ii) discharge/migration routes, and (iii) contaminant distribution in particle size fraction and settling velocity of the soils. The results of investigation showed that TNT and RDX are the major contaminants but the extent of contamination varied depending on the types of military training practices and topography of the site. RDX was also detected in the subsurface soil and in the nearby stream within the training ranges, suggesting release of contaminants to streams. The median concentrations of explosives in the surface soil were less than 20 mg/kg despite several 'hot spots' in which explosives concentrations often exceeds several hundred mg/kg. The average clay contents in the soil of target area was less than 5 % compared to 12 % in the control, indicating loss of smaller particles by surface runoff during rainfall due to lack of vegetative land cover. Analysis of explosive compounds and particle size distribution showed that the amount of explosive compounds in soil particles smaller than 0.075 mm was less than 10 % of the total. Settling column tests also revealed that the quantity of explosive compounds in the liquid phase of the effluent was greater than that in the solid phase. Therefore, pre-treatment of particulate matter in surface runoff of shooting range with a simple settling basin and subsequent effluent treatment with planted constructed wetlands as polishing stage for explosives in the aqueous phase would provide the shooting ranges with a self-standing, sustainable, green solution.

Case History of Sea Dyke Filter Construction Using Geotextile Tube Mattress (튜브형 매트리스를 활용한 방조제 필터공 축조사례연구)

  • Oh, Young-In;Yoo, Jeon-Yong;Kim, Hyun-Tae
    • Journal of the Korean Geosynthetics Society
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    • v.6 no.1
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    • pp.9-16
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    • 2007
  • Geotextile is one of the most useful and effective polymer material in civil construction works and the main function of geotextile is separation, reinforcement, filtering and drainage. Recently, because of the shortage of natural rock, traditional forms of river and coastal structures have become very expensive to build and maintain. Therefore, the materials used in hydraulic and coastal structures are changing from the traditional rubble and concrete systems to the cheaper materials and systems. One of these alternatives employs geotextile tube technology in the construction of coastal and shore protection structures, such as embankment, see dyke, groins, jetties, detached breakwaters and so on. Geotextile tube technology has changed from being an alternative construction technique and, in fact, has advanced to become the most effective solution of choice. This paper presents case history of sea dyke filter construction using geotextile tube mattress and also, various issues related to the tube mattress design and construction technology.

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Development of a 2 Dimensional Numerical Landscape Evolution Model on a Geological Time Scale (2차원 지질시간 규모 수치지형발달모형의 개발)

  • Byun, Jong-Min;Kim, Jong-Wook
    • Journal of the Korean Geographical Society
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    • v.46 no.6
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    • pp.673-692
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    • 2011
  • Advances in computer technology have enabled us to develop and use numerical landscape evolution models (NLEMs) for exploring the dynamics of geomorphic system from a variety of viewpoints which previously could have not been taken. However, as of yet there have been no trials using or developing NLEMs in Korea. The purpose of this research is to develop a 2 dimensional NLEM on a geological time scale and evaluate its usefulness. The newly developed NLEM (ND-NLEM) treats bedrock weathering as one of the major geomorphic processes and attempts to simulate the thickness of soil. As such it is possible to model the weathering-limited as well as the transport-limited environment on hillslopes. Moreover the ND-NLEM includes not only slow and continuous mass transport like soil creep, but also rapid and discrete mass transport like landslides. Bedrock incision is simulated in the ND-NLEM where fluvial transport capacity is large enough to move all channel bed loads, such that ND-NLEM can model the detachment-limited environment. Furthermore the ND-NLEM adopts the D-infinity algorithm when routing flows in the model domain, so it reduces distortion due to the use of the steepest descent slope flow direction algorithm. In the experiments to evaluate the usefulness of the ND-NLEM, characteristics of the channel network observed from the model results were similar to those of the case study area for comparison, and the hypsometry curve log during the experiment showed rational evidence of landscape evolution. Therefore, the ND-NLEM is shown to be useful for simulating landscape evolution on a geological time scale.

The Simulation of Flood Inundation of Namdae Stream with GIS-based FLUMEN model (GIS 기반 FLUMEN 모형을 이용한 남대천 홍수범람 모의실험)

  • Lee, Geun-Sang;Choi, Yun-Woong
    • Spatial Information Research
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    • v.18 no.2
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    • pp.25-34
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    • 2010
  • This study simulated flood inundation each frequency rainfall using GIS spatial information and FLUMEN model for part of Muju-Namdae Stream. To create geomorphology for the analysis of flood inundation, Triangle Irregular Network(TIN) was constructed using GIS spatial interpolation method based on digital topographic map and river profile data, unique data source to represent real topography of the river areas. And also flood inundation was operated according to the levee collapse to consider extremely flood damage scenarios. As the analysis of result, the inundation area in the left levee collapse showed more high as 3.13, 3.69, and 4.17 times comparing with one of right levee for 50, 100, and 200 year frequency rainfall and showed 1.00, 2.15, and 3.34 times comparing with one of right levee in the inundation depth with over 1.0 meter, which can cause casualties. As the analysis of inundation area of the inundation depth with over 1.0 meter, which can cause casualties in left levee collapse, it increased more high as 263% and 473% when 50 year frequency change into 100 and 200 year frequency. Also As the analysis of inundation area of the inundation depth with over 1.0 meter in right levee collapse, it increased high as 123% and 142% when 50 year frequency change into 100 and 200 year frequency. Especially, the inundation area of the inundation depth with 3.0~3.5m showed more high as 263% and 489% when 50 year frequency change into 100 and 200 year frequency. It is expected that flood inundation map of this paper could be important decision making data to establish land use planning and water treatment measures.

Development of the Modeling Technique for Soil Loss Reduction in Highland Area using the SWAT Model (SWAT 모형을 이용한 고랭지 지역에서의 토양유실 저감 모델링 기법 개발)

  • Jang, Won-Seok;Choi, Joong-Dae;Park, Youn-Shik;Lim, Kyoung-Jae
    • Proceedings of the Korea Water Resources Association Conference
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    • 2010.05a
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    • pp.1457-1460
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    • 2010
  • 최근 기후변화로 인해 태풍 등과 같은 집중성 강우가 많이 발생하고 있고, 강우시 유출 등으로 인한 유역내 발생하는 토양침식으로 인해 유역의 하천을 비롯하여 유출구에서의 탁수 및 유사 발생 문제는 우리나라 뿐만 아니라 전 세계적으로 심각한 환경문제로 대두되고 있다. 강우시 유역에서 발생하는 이러한 탁수문제를 효과적으로 해결하기 위하여 여러 최적관리기법 (Best Management Practices, BMPs) 들이 제안되어왔다. 본 연구의 목적은 볏짚매트 사용으로 인한 토양유실 저감 실측자료를 바탕으로 볏짚매트를 다양한 경사지 밭에 적용하였을 경우 유역에서의 토양유실 저감효과를 평가하기 위한 모델링 기법을 개발하는데 있다. 볏짚 매트의 효과를 Soil and Water Assessment Tool (SWAT) 모형으로 모의하기 위해서는 볏짚매트의 효과를 모형에서 반영할 수 있는 인자를 선택하여 최적의 인자값을 산정해야 한다. 본 연구에서는 유사 저감 효과 실험 결과를 VFSMOD-W 모형을 이용하여 USLE P 인자값을 도출하였으며, 경사도에 따른 USLE-P 값을 산정하여, SWAT 모형에 입력자료로 사용하였다. 분포형 모형과는 달리 준분포형 모형인 SWAT 모형은 소유역내 수문학적 반응단위별로 유출, 유사, 그리고 비점오염 발생을 평가하는데 이때 Hydrologic Response Unit (HRU)의 지형정보가 활용된다. 이 지형정보는 SWAT 유사 평가시 매우 민감한 변수중의 하나이기 때문에 유역 단위 유사 평가시 정확한 지형자료의 입력이 요구된다. 그러나 SWAT 모형은 소유역내 HRU의 경사도 및 경사장을 직접 산정하지 않고, 소유역의 평균경사도를 기준으로 하여 산정된 경사도를 소유역내 모든 HRU에 동일하게 적용하는 단점이 있다. 본 연구에서는 준분포 모형인 SWAT 모형의 단점을 개선하기 위하여 SWAT Spatially Distributed (SD)-HRU를 적용하였다. 이를 통해 다양한 경사지 밭에서의 볏짚매트의 효과를 분석할 수 있게 되었다. 볏짚매트 미적용시 모의 기간내 유사량 총합은 74,954.42 ton 이고, 월평균 유사량은 814.72 ton 으로 산정되었고, 볏짚매트를 적용하였을 경우 모의 기간내 (2000년 1월 - 2007년 8월) 유사량 총합은 48,460.55 ton 이고, 월평균 유사량은 526.75 ton 으로 볏짚매트를 적용하지 않았을 때보다 약 35.35 % 저감된 값을 보였다. SD-HRU를 적용하고 각 농경지의 경사도에 따라 USLE P 값을 수정하여 볏짚매트에 의한 효과를 분석하였을 때, 볏짚매트를 적용하지 않았을 때 보다 볏짚매트를 적용하였을 때 모의 기간내 약 35% 정도의 유사량이 감소된 것을 알 수 있었다. 본 연구결과에서 보여주는 바와 같이 고랭지 지역에서의 영농활동 시 볏짚매트를 설치한다면 강우시 발생하는 토양유실을 효과적으로 저감시킬 수 있을 것이라 판단된다.

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Impacts on Water Quality to an Artificial Lake Due to Sudden Disturbance of Sediments (급격한 저니토 교란이 인공 하구호 수질에 미치는 영향)

  • 서승원;김정훈
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.15 no.1
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    • pp.39-50
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    • 2003
  • This study aims to figure out water quality impacts due to sudden disturbances of sediments during dike construction in land reclamation for the northern part of the Siwhaho Lake where heavily deteriorated settlements from upstream loadings are outstanding. We exploit a 3-D water quality model CE-QUAL-ICM combined with a hydrodynamic model TIDE3D. Simulations are done accounting water-sediment interaction in a 4-$\sigma$ layers. Long-term simulation for 1-year shows that bottom layers around the disturbance location are only affected and marks very high concentration. Complete vertical mixing appears at least 5km apart to downward due to complex effects of geometry, bathymetry and river inflows. It should be addressed that existing condition of the Siwhaho Lake stands for high concentration of COD and TP in winter and spring due to relatively high incoming loadings, however the effect of sediment disturbances yields reverse phenomena, i.e., impacts of dike construction arise greatly in summer and fall. Refined grid system consisting of 150m${\times}$150m rectangular grid, which is doubled system compared to previous study (Suh et al.,2002), gives affordable results by reducing flux differences through a cell especially in front of gate.

Use of Light Emitting Diode for Enhanced Activity of Sulfate Reducing Bacteria in Mine Drainage Treatment Process Under Extreme Cold (혹한기 광산배수 처리 공정 내 황산염 환원 박테리아의 활성 증진을 위한 발광다이오드의 이용 제안)

  • Choi, Yoojin;Choi, Yeon Woo;Lee, An-na;Kim, Kyoung-Woong
    • Economic and Environmental Geology
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    • v.50 no.3
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    • pp.251-256
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    • 2017
  • This study presents measures to enhance the efficiency of Successive Alkalinity Producing Systems(SAPS), a natural biological purification method that prevents environmental pollution arising from the release of Acid Mine Drainage(AMD) from abandoned mines into rivers and groundwater. The treatment of AMD using SAPS is based on biological processing technology that mostly involves sulfate reducing bacteria(SRB). It has been proven effective in real-world applications, and has been employed in various projects on the purification of AMD. However, seasonal decrease in temperature leads to a deterioration in the efficiency of the process because sulfate-reducing activity is almost non-existent during cold winters and early spring even if SRB is able to survive. Against this backdrop, this study presents measures to enhance the activity of the SRB of the organic layer by integrating light emitting diode(LED)s in SAPS and to maintain the active temperature using LEDs in cold winters. Given that mine drainage facilities are located in areas where power cannot be easily supplied, solar cell modules are proposed as the main power source for LEDs. By conducting further research based on the present study, it will be possible to enhance the efficiency of AMD treatment under extreme cold weather using solar energy and LEDs, which will serve as an environmentally-friendly solution in line with the era of green growth.