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Inhibitory Effect of Potato Sprouting Inhibitor Chlorpropham on Dry Rot (감자 맹아억제제 Chlorpropham의 마른썩음병 억제 효과)

  • Kyusuk Han;Byung Sup Kim;Sae Jin Hong;Nam Sook Kim
    • Journal of Bio-Environment Control
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    • v.32 no.2
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    • pp.165-171
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    • 2023
  • Potato dry rot is one of the potato storage diseases caused by Fusarium species and is a representative pathological disorder that induced post-harvest loss during storage. Chlorpropham treatment for sprouting inhibition is mainly used for room temperature storage of potatoes for processing. In this study, the inhibitory effect of chlorpropham on Fusarium-induced dry rot of potato 'Dano'. To investigate the mycelial growth rate of the dry rot fungus (Fusarium solani Appel & Wollenw), mycelial growth was investigated in a chlorpropham (5.0, 50.4, 503.8, and 5,038 ppm) and prochloraz (0.1, 1.0, 10.0, and 100.0 ppm) medium containing F. oxysporum mycelia. Mycelia were more inhibited as the concentration of chlorpropham and prochloraz increased during incubation at 20℃, and the inhibition rate was 98.2% and 100% when treated with 503.8 ppm of chlorpropham and 10ppm of prochloraz in 14 days, respectively. Potato Dano tubers inoculated with F. oxysporum were dipped in chlorpropham (5.0, 50.4, and 503.8 ppm) and prochloraz (100 ppm) to investigate the effect of preventing dry rot during cold storage at 20℃ and 4℃ in vivo. The disease diameter of potatoes stored at room temperature (about 20℃) was reduced to 13.0 mm in the prochloraz 100 ppm teatment, and 10.7 mm in the chlorpropham 50.4 ppm treatment compared to 13.7 mm in the control tuber at 70 days of storage. The disease progression in all treatments including control was similar with no statistically significant difference at 4℃ air temperature. From the results of this study, it is considered that treatment with 50.4 ppm of chlorpropham after harvest will be useful for suppressing dry rot of stored potatoes.

Ecological Network on Benthic Diatom in Estuary Environment by Bayesian Belief Network Modelling (베이지안 모델을 이용한 하구수생태계 부착돌말류의 생태 네트워크)

  • Kim, Keonhee;Park, Chaehong;Kim, Seung-hee;Won, Doo-Hee;Lee, Kyung-Lak;Jeon, Jiyoung
    • Korean Journal of Ecology and Environment
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    • v.55 no.1
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    • pp.60-75
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    • 2022
  • The Bayesian algorithm model is a model algorithm that calculates probabilities based on input data and is mainly used for complex disasters, water quality management, the ecological structure between living things or living-non-living factors. In this study, we analyzed the main factors affected Korean Estuary Trophic Diatom Index (KETDI) change based on the Bayesian network analysis using the diatom community and physicochemical factors in the domestic estuarine aquatic ecosystem. For Bayesian analysis, estuarine diatom habitat data and estuarine aquatic diatom health (2008~2019) data were used. Data were classified into habitat, physical, chemical, and biological factors. Each data was input to the Bayesian network model (GeNIE model) and performed estuary aquatic network analysis along with the nationwide and each coast. From 2008 to 2019, a total of 625 taxa of diatoms were identified, consisting of 2 orders, 5 suborders, 18 families, 141 genera, 595 species, 29 varieties, and 1 species. Nitzschia inconspicua had the highest cumulative cell density, followed by Nitzschia palea, Pseudostaurosira elliptica and Achnanthidium minutissimum. As a result of analyzing the ecological network of diatom health assessment in the estuary ecosystem using the Bayesian network model, the biological factor was the most sensitive factor influencing the health assessment score was. In contrast, the habitat and physicochemical factors had relatively low sensitivity. The most sensitive taxa of diatoms to the assessment of estuarine aquatic health were Nitzschia inconspicua, N. fonticola, Achnanthes convergens, and Pseudostaurosira elliptica. In addition, the ratio of industrial area and cattle shed near the habitat was sensitively linked to the health assessment. The major taxa sensitive to diatom health evaluation differed according to coast. Bayesian network analysis was useful to identify major variables including diatom taxa affecting aquatic health even in complex ecological structures such as estuary ecosystems. In addition, it is possible to identify the restoration target accurately when restoring the consequently damaged estuary aquatic ecosystem.

Selection Indices to Identify Drought-tolerance and Growth Characteristics of the Selected Korean Native Plants (자생식물로부터 내건성 식물의 최적인자 선발과 생육특성)

  • Im, Hyeon Jeong;Song, Hyeon Jin;Jeong, Mi Jin;Seo, Yeong Rong;Kim, Hak Gon;Park, Dong Jin;Yang, Woo Hyung;Kim, Yong Duck;Choi, Myung Suk
    • Journal of agriculture & life science
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    • v.50 no.2
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    • pp.73-82
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    • 2016
  • Best drought tolerance index was determined through statistics analysis and growth appearance of drought tolerant plants was determined by cultivation in pot and sloping land. For determination of best drought tolerant indicators, RD(Resistant dry days), LD(Leaf area), UTR(Unit transpiration), RWC(Relative water content), RWL(Relative water loss), LA(Leaf area), SN(Stoma unmber) and SA(Stoma area) were carried out by correlation and PCA analysis. RWL and UTR were affected on plant drought tolerance according to comparison among six indices for resistant dry days. The PCs axes separated SA, LA, RD and RWC and SN. UTR was negatively correlated with SA, RWL were also negatively correlated with RWC and SN. RWL and UTR were proved best selection indicator for the selection of drought tolerant species. Ulmus parvifolia, Bidens bipinnata, Patrinia villosa, Kummerowia striata, Arundinella hirta, Artemisia gmelini etc. were selected drought tolerant plants. Shoot growth appearance of drought resistant plants was differed pot and sloping land. Shoot growth and leaf number was no significant differences between the pot and sloping land. However, root growth of drought tolerant plants was all the difference between two cultivation. T/R ratio of drought tolerant plants was also found a big difference. T/R ratio of drought tolerant plants in sloping land was lower than that of pot. These results will be served efficiently plant breeding.

Growth, Productivity and Forage Values of Winter Cereal Crops at Paddy Fields in the Southern Region of Korea (남부지역 논에서 동계 맥류의 생육특성, 생산성 및 사료가치)

  • Seo Young Oh;Jong Ho Seo;Jisu Choi;Tae Hee Kim;Seong Hwan Oh
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.2
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    • pp.61-70
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    • 2023
  • In order to select high-quality winter cereal crops with high yield and to increase self-sufficiency rate of forage, their growth, yield, and feeding value of several cereal crops cultivated in winter were investigated in the paddy field of the southern region. Four wheat cultivars and green barley headed in early and mid-April, while oat and Italian ryegrass headed in early May. Fresh forage yields of wheats, green barley, and oat were significantly higher than that of Italian ryegrass, and dry forage yields of wheats and green barley were significantly higher than those of not only Italian ryegrass but also oat. In particular, the yield of a wheat cultivar 'Cheongwoo' was the highest. Mineral contents of wheat forages, even though low, were in the range 27.8~33.7mg·g-1 DW suitable for feeding cattle and young female cows. Crude protein content of a wheat cultivar 'Cheongwoo' was high up to 7.6%, similarly to 7.0% requiring for feeding cattle. Feeding values such as total digestible nutrients (TDN) and relative feed value (RFV) of wheats and green barley were superior to those of oat and Italian ryegrass. In addition, dry matter rates of 4 wheat cultivars and green barley were in the range 30~40%, indicating that wheat cultivars and green barley could be used for various feeding purposes such as green or dried forage, and silage. Based on these results, wheat cultivars including 'Cheongwoo' and green barley could be encouraged to be cultivated in paddy fields, as high-quality winter forage crops with high yield.

Determining the Authenticity of Labeled Traceability Information by DNA Identity Test for Hanwoo Meats Distributed in Seoul, Korea (DNA 동일성 검사를 통한 서울지역 유통 한우육의 표시 이력정보 진위 판별)

  • Yeon-jae Bak;Mi-ae Park;Su-min Lee;Hyung-suk Park
    • Journal of Food Hygiene and Safety
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    • v.38 no.1
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    • pp.12-18
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    • 2023
  • Beef traceability systems help prevent the distribution of Hanwoo (Korean native cattle) meat as imported beef. In particular, assigning a traceability number to each cattle can provide all information regarding the purchased Hanwoo meat to the consumers. In the present study, a DNA identity test was conducted on 344 samples of Hanwoo meat from large livestock product stores in Seoul between 2021 and 2022 to determine the authenticity of important label information, such as the traceability number. Traceability number mismatch was confirmed in 45 cases (13.1%). The mismatch rate decreased to 11.3% in 2022 from 14.7% in 2021, and the mismatch rate was higher in the northern region (16.9%) than in the southern region (10.2%). In addition, of the six brands, B and D showed satisfactory traceability system management, whereas E and A showed poor traceability system management, with significant differences (P<0.001). The actual traceability number confirmation rate was only 53.9% among the mismatch samples. However, examination of the authenticity of label information of the samples within the identified range revealed false marking in the order of the traceability number (13.1%), sex (2.9%), slaughterhouse name (2.2%), and grade (1.6%); no false marking of breed (Hanwoo) was noted. To prevent the distribution of erroneously marked livestock products, the authenticity of label information must be determined promptly. Therefore, a legal basis must be established mandating the filling of a daily work sheet, including the traceability number of beef, in partial meat subdivisions. Our findings can be used as reference data to guide the management direction of traceability systems for ensuring transparency in the distribution of livestock products.

Floristic Study of Sangwangsan Mt. and Its Adjacent Areas(Wando-gun) (완도 상왕산 일대의 식물상 연구)

  • Gwang-Il Kim;Chan-jin Oh;Sun-jin Lee;Soon-Ho Shin;Kyoung-Pae Yun
    • Korean Journal of Environment and Ecology
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    • v.37 no.2
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    • pp.100-139
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    • 2023
  • This study was intended to identify the distribution and characteristics of plants such as native plants, rare plants, and endemic plants through a flora survey in Sangwangsan Mt. (644m), Wando-gun, Jeollanam-do, a group habitat of warm temperate forests in Korea, and use the data for the conservation of plant species diversity and the study of climate and distribution changes in warm-temperate forests. A total of 32 field surveys were conducted from 2018 to 2022. The survey identified 785 taxa, including 8 forms, 53 varieties, 16 subspecies, 708 species, 473 genera, and 132 families. The endangered wild plants designated by the Ministry of Environment included 6 taxa: Woodwardia japonica, Metanarthecium luteoviride, Bulbophyllum inconspicuum, Dendrobium moniliforme, Pelatantheria scolopendrifolia, and Cymbidium macrorhizon. Rare plants designated by the Korea Forest Service were identified as 26 taxa. The red list designated by the Korea National Arboretum was identified as 7 taxa, the red list designated by the Ministry of Environment was identified as 29 taxa, and endemic plants in Korea were identified as 17 taxa. Floristic target species in Korea were identified as 200 taxa, specifically 6 taxa of grade V, 13 taxa of grade IV, 73 taxa of grade III, 29 taxa of grade II, and 79 taxa of grade I. Naturalized plants were identified as 73 taxa, and invasive alien plants were identified as 6 taxa. Target plants adaptable to climate change in Korea were identified as 55 taxa, specifically 8 taxa of endemic plants, 46 taxa of southern plants, and 1 taxon of northern plants.

Distribution and Frequency of SSR Motifs in the Chrysanthemum SSR-enriched Library through 454 Pyrosequencing Technology (국화 SSR-enriched library에서 SSR 반복염기의 분포 및 빈도)

  • Moe, Kyaw Thu;Ra, Sang-Bog;Lee, Gi-An;Lee, Myung-Chul;Park, Ha-Seung;Kim, Dong-Chan;Lee, Cheol-Hwi;Choi, Hyun-Gu;Jeon, Nak-Beom;Choi, Byung-Jun;Jung, Ji-Youn;Lee, Kyu-Min;Park, Yong-Jin
    • Journal of the Korean Society of International Agriculture
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    • v.23 no.5
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    • pp.546-551
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    • 2011
  • Chrysanthemums, often called mums or chrysanths, belong to the genus Chrysanthemum, which includes about 30 species of perennial flowering plants in the family Asteraceae. We extracted DNA from Dendranthema grandiflorum ('Smileball') to construct a simple sequence repeat (SSR)-enriched library, using a modified biotin-streptavidin capture method. GS FLX (Genome Sequencer FLX System which provides the flexibility to perform the broad range of applications) sequencing (at the 1/8 run specification) resulted in 18.83 mega base pairs (Mbp) with an average read length of 280.06 bp. Sequence analyses of all SSR-containing clones revealed a predominance of di-nucleotide motifs (16,375, 61.5%) followed by tri-nucleotide motifs (6,616, 24.8%), tetra-nucleotide motifs (1,674, 6.3%), penta-nucleotide motifs (1,283, 4.8%), and hexa-nucleotide motifs (693, 2.6%). Among the di-nucleotide motifs, the AC/CA class was the most frequently identified (93.5% of all di-nucleotide types), followed by the GA/AG class (6.1%), the AT/TA class (0.4%), and the CG/GC class (0.03%). When we analyzed the distribution of different repeat motifs and their respective numbers of repeats, regardless of the motif class, of 100 SSR markers, we found a higher number of di-nucleotide motifs with 70 to 80 repeats; we also found two di-nucleotide motifs with 83 and 89 repeats, respectively, but their product lengths were within optimum size (297 and 300 bp). In future work, we will screen for polymorphisms of possible primer pairs. The results will provide a useful tool for assessing molecular diversity and investigating the population structure among and within Chrysanthemum species.

Yield, Nitrogen Use Efficiency and N Uptake Response of Paddy Rice Under Elevated CO2 & Temperature (CO2 및 온도 상승 시 벼의 수량, 질소 이용 효율 및 질소 흡수 반응)

  • Hyeonsoo Jang;Wan-Gyu Sang;Youn-Ho Lee;Pyeong Shin;Jin-hee Ryu;Hee-woo Lee;Dae-wook Kim;Jong-tag Youn;Ji-Won Han
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.346-358
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    • 2023
  • Due to the acceleration of climate change or global warming, it is important to predict rice productivity in the future and investigate physiological changes in rice plants. The research aimed to explore how rice adapts to climate change by examining the response of nitrogen absorption and nitrogen use efficiency in rice under elevated levels of carbon dioxide and temperature, utilizing the SPAR system for analysis. The temperature increased by +4.7 ℃ in comparison to the period from 2001 to 2010, while the carbon dioxide concentration was held steady at 800 ppm, aligning with South Korea's late 21st-century RCP8.5 scenario. Nitrogen was applied as fertilizer at rates of 0, 9, and 18 kg 10a-1, respectively. Under conditions of climate change, there was an 81% increase in the number of panicles compared to the present situation. However, grain weight decreased by 38% as a result of reduction in the grain filling rate. BNUE, indicative of the nitrogen use efficiency in plant biomass, exhibited a high value under climate change conditions. However, both NUEg and ANUE, associated with grain production, experienced a notable and significant decrease. In comparison to the current conditions, nitrogen uptake in leaves and stems increased by 100% and 151%, respectively. However, there was a 25% decrease in nitrogen uptake in the panicle. Likewise, the nitrogen content and NDFF (Nitrogen Derived from Fertilizer) in the sink organs, namely leaves and roots, were elevated in comparison to current levels. Therefore, it is imperative to ensure resources by mitigating the decrease in ripening rates under climate change conditions. Moreover, there seems to be a requirement for follow-up research to enhance the flow of photosynthetic products under climate change conditions.

Comparison of Fruit Characteristics of 'Fuji'/M.26 in Response to Ethephon Treatment and Combined Treatment of Ethephon and CaCl2 During Maturing Stages (Ethephon 단용처리와 Ethephon 및 염화칼슘 혼합처리에 따른 사과 'Fuji'/M.26의 성숙기 과실특성 비교)

  • Sewon Oh;Seong Ho Moon;Keum-Il Jang;Junsoo Lee;Daeil Kim
    • Korean Journal of Plant Resources
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    • v.36 no.5
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    • pp.517-526
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    • 2023
  • The harvest time of the late-ripening 'Fuji' apple (Malus × domestica) is variable, depending on the coloration of the fruit skin. Ethephon, a plant growth regulator, promotes the ethylene production and induces physiological responses associated with fruit maturation in climacteric fruit crops, such as apples. This study aimed to investigate the effect of ethephon treatment on fruit characteristics after fruit enlargement, with the objective of proposing an economical and stable harvest control method for 'Fuji'/M.26 apples. Fruit characteristics were assessed at 10-days intervals following the application of 100 mg/L ethephon and mixture of 100 mg/L ethephon and 0.5% CaCl2 at 145 days after full bloom (DAFB). Starch contents of ethephon-treated (ET) and ethephon with CaCl2-treated (EC) apples began to decrease from 155 DAFB, and the starch contents of ET and EC at 10 days before harvest were similar to those of control at harvest time. Red coloration of fruit skin in EC was lower compared to ET but higher than control. The average fruit firmness was low in ET, while the control and EC exhibited similar levels of firmness. Fruit sugar acid ratios did not show significant differences between treatments. However, the titratable acidity of EC was significantly lower than that of the control at 10 days before harvest. Moreover, fruit sugar acid ratio of ET and EC at 10 days before harvest in 2021 was similar to their sugar acid ratio at harvest time. Therefore, it was thought that fruit maturation and skin coloration could be accelerated by 10 days from the harvest time through the combined treatment of 100 mg/L ethephon and 0.5% CaCl2 at the end of fruit enlargement in 'Fuji'/M.26.

Effect of Drying Type and Addition Level of Sweet Potato 'Tongchaeru' Byproducts on Broiler Productivity, Meat Quality, Blood Parameters, and Immune Response (고구마 '통채루' 부산물의 건조 방법과 첨가 수준에 따른 육계 생산성, 계육 품질, 혈액 성상, 면역 지표에 미치는 영향)

  • Woo-Do Lee;Hyunsoo Kim;Jiseon Son;Eui-Chul Hong;Hee-Jin Kim;Yeon-Seo Yun;Hye Kyung Shin;Hwan-Ku Kang
    • Korean Journal of Poultry Science
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    • v.50 no.4
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    • pp.325-336
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    • 2023
  • This study used leaves and stems of 'Tongchaeru', one of the sweet potato varieties, to investigate broiler productivity, meat quality, blood properties, growth hormones, and immune factor levels according to drying method and amount added to feed. For this experiment, a total of 720 1-day-old male Ross 308 broilers were used. Treatments were assigned with 3 replicates per treatment and 20 birds were assigned to each replicate. The treatment group was designed into 12 treatments according to the type of natural product (leaves (L), stems (S)), drying type (natural (N), hot air (H), freeze (F)) and amount added (0.1%, 0.3%). The test was conducted for a total of 5 weeks. In this study, there was no significant difference in productivity depending on the type and amount of additives added (P>0.05). The FS 0.3% group showed high pH and WHC levels, and the shear force was lowest at HL 0.1% group (P<0.05). Blood cell and serum biochemical components were similar in all treatments, and growth hormone IGF-1 was highest in FS 0.1% group (P<0.05). There was no significant difference in IFN-γ, but the highest level of IL-6 was seen in the HS 0.1% group (P<0.05). In conclusion, the meat quality and the level of growth hormone and immune factors in the body were different depending on the type and amount of dried leaves and stems of sweet potato 'Tongchaeru', further study is needed to compare the selected additives and amounts added with those without additions.