• Journal of Gastric Cancer
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• v.9 no.3
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• pp.136-142
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• 2009

Purpose: This clinical study was conducted to evaluate the predictive value of tumor markers for recurrence and the clinical significance of false positive findings after curative gastrectomy in patients with gastric cancer. Materials and Methods: Two hundred ninety patients with gastric cancer who underwent gastrectomy with curative intent were evaluated retrospectively. We analyzed the correlations between changes in tumor markers (CEA, CA 19-9, AFP, and CA-125) and clinicopathologic data, and basis for changes in tumor markers without recurrence during the follow-up period. Results: The sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of tumor markers for recurrence were 75.0%, 64.6%, 23.1%, 94.8%, and 65.9% respectively. Among 36 patients with recurrences, 10 patients (27.8%) had elevated tumor markers prior to positive findings on imaging studies, while 13 patients (36.1%) had concomitant elevation in tumor markers. At least 1 of the 4 tumor markers increased in 90 of 290 patients during the follow-up period; however, there was no evidence of tumor recurrence. Twenty patients had persistently elevated tumor markers, while the tumor marker levels in 70 patients returned to normal level within $9.08\pm7.2$ months. The patients with pulmonary disease, hepatobiliary disease, diabetes, hypertension, or herbal medication users had elevated tumor markers more frequently than patients without disease (P<0.001). Conclusion: Although detecting recurrence of gastric cancer with tumor markers may be useful, false positive findings of tumor markers are common, so surgeons should consider other chronic benign diseases and medical conditions when tumor markers increase without evidence of recurrence.

• Journal of Chest Surgery
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• v.43 no.5
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• pp.499-505
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• 2010

Background: There have been several reports using animal experiments that CD1-restricted T-cells have a key role in tumor immunity. To address this issue, we studied the expression of markers for CD1c+ myeloid dendritic cells (DCs) isolated from peripheral blood in the clinical setting. Material and Method: A total of 24 patients with radiologically suspected or histologically confirmed lung cancer who underwent pulmonary resection were enrolled in this study. The patients were divided according to histology findings into three groups: primary adenocarcinoma of lung (PACL), primary squamous cell carcinoma of lung (PSqCL) and benign lung disease (BLD). We obtained 20 mL of peripheral venous blood from patients using heparin-coated syringes. Using flow-cytometry after labeling with monoclonal antibodies, data acquisition and analysis were done. Result: The ratio of CD1c+CD19- dendritic cells to CD1c+ dendritic cells were not significantly different between the three groups. CD40 (p=0.171), CD86 (p=0.037) and HLA-DR (p=0.036) were less expressed in the PACL than the BLD group. Expression of CD40 (p=0.319), CD86 (p=0.036) and HLA-DR (p=0.085) were less expressed in the PACL than the PSqCL group, but the differences were only significant for CD86. Expression of co-stimulatory markers was not different between the PSqCL and BLD groups. Expression of markers for activated DCs were dramatically lower in the PACL group than in groups with other histology (CD40 (p=0.005), CD86 (p=0.013) HLA-DR (p=0.004). Conclusion: These results suggest the possibility that CD1c+ myeloid DCs participate in control of the tumor immunity system and that low expression of markers results in lack of an immune response triggered by dendritic cells in adenocarcinoma of the lung.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.91-96
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• 2006

현대의학의 발전으로 많은 질병들의 치료율이 개선되고 있으나, 암은 여전히 낮은 치료율과 약제 내성 및 부작용으로 많은 환자들이 의학적 고통 뿐 아니라 정신적, 경제적 문제점들을 호소하고있다. 이와같은 문제점은 동일한 병리학적 특성을 가지는 종양이라도 사람마다 그 생물학적 특성이 다르며, 동일한 환자안에서도 종양의 시기에 따라 다양한 특성의 세포들이 공존하며 다양한 문제를 발생하는 tumor heterogeneity에서 기인하게된다. 다행히 최근의 분자생물학의 발전과 인간유전체연구들의 활성화로 이와같은 다양한 암의 특성과 환자들의 특성을 이해하는 연구 방법들의 개발로 환자의 특성에 맞는 항암제를 효율적으로 투여하는 맞춤치료를 향한 노력을 지속하고 있다. 이와같은 맞춤치료의 일환으로 약제의 환자에서의 반응과 부작용을 예측하고자 최신의 high-throughput 기법을 도입한 것이 Pharmacogenomics이다. 즉, 지금까지의 항암치료는 암의 종류에 따라 임상연구 결과에 근거한 항암제를 선택하고 있다. 그러나 앞서 설명한 것처럼 암의 특성과 환자 반응의 다양화로 실제 항암효과는 기대에 미치지 못하여 많은 수의 환자들이 치료에 내성을 보일 뿐 아니라 치명적인 부작용으로 새로운 문제에 대면하게 되었다. 따라서 각 항암제011 최대의 효과를 보이며 최소의 부작용을 나타내는 최선의 치료책을 선정하는 것이 중요한 과제이다. 이를 위해서 암환자의 치료 단계에서 정확한 진단 및 병기 설정, 생물학적 특성 이해 뿐 아니라, 치료 반응을 예측할 수 있는 생물학적 표지자를 찾고자 하는 노력의 결과로 현재 임상에 사용되는 몇 가지 종양표지자를 포함하여 다양한 유전자 칩들이 연구단계에 있다. 특히 다양한 생물학적 현상이 많은 유전자들의 변화에 의한다는 근거하여 약제의 효과와 부작용을 예측할 수 있는 표지자 발굴도 DNA chip 등의 high-throughput technology를 사용하여 그 특이도와 민감도가 향상된 표지자 발굴이 시도되고 있다. 아직은 시작단계이고 많은 검증이 필요하나 여러 가지 가능성의 증거들로 멀지않은 시기에 맞춤치료가 가능하리라 기대하며, 암 연구에 있어서 pharmacogenomics의 현황을 소개하고자 한다.

• The Journal of the Korean bone and joint tumor society
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• v.7 no.2
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• pp.41-50
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• 2001

Purpose : To investigate biochemical markers for osteosarcoma, activities of deoxyribocuclease(DNase), ribonuclease(RNase), 5'-nucleotidase, alkaline phosphatase and amylase were determined in the osteosarcoma tissue and serum of patients with osteosarcoma. Also studied were DNase, RNase in osteosarcoma tissue, isolating the enzymes from the sarcoma tissue and investigating the sarcoma specific enzymes. Materials and Methods : The experimental tissue and serum were obtained from twelve patients with osteosarcoma. The control group were obtained from the normal healthy tissue of the same patients. The tissue were centrifugalized to obtain extracts. The extracts were analized for the estimation of nucleic acid, protein contents and enzyme activities. And then each enzymes were isolated and analized by DEAE-cellulose chromatography and estimated for activities. Result : Activities of acid DNase, RNase, 5'-nucleotidase and alkaline phosphatase were significantly increased in osteosarcoma tissue. Neutral RNase in osteosarcoma tissue was shown to bo highly active, exhibiting secretory form of RNase inhibitor associated with the RNase was also increased. In the serum of patients with osteosarcoma, RNase activity was significantly increased. DEAE-cellulose column chromatographical analysis revealed that acid DNase was isolated as a single enzyme and neutral RNase as five isozymes in osteosarcoma tissue. Conclusion : The results indicated that combination of these enzymes could be used as markers for osteosarcoma. The results indicated that acid DNase and neutral RNase might play a role in genesis of sarcoma and suppression of sarcoma.

• Journal of Korean Society of Forest Science
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• v.95 no.4
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• pp.435-442
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• 2006

A total of 167 peculiar haplotypes confirmed from 28 cpSR variants that were observed in 19 populations of Japanese red pine in Korea through cpSSR marker analysis. Thirteen individuals that showed identical haplotype dispersed evenly in 10 populations, and the average number of effective haplotype within population was 13.37. Estimate of genetic diversity (He) was 0.987 on the basis of cpSSR haplotype variants that was equivalent to or higher than the estimates reported in other studies on some forest tree species. Estimation of genetic diversity (S.I.) on the basis of cpSSR variants composing each haplotype revealed the highest estimate of 1.109 for the population of Gangwon-Yeongwol and the lowest estimate of 0.411 for the population of Gyeongbuk Mungyeong with the average of 0.887. Most of observed cpSSR variants appeared to exist commonly in 19 populations (97.62%), and genetic differentiation of cpSSR variants among populations was turned out to be weak (${\Phi}_{ST}=0.024$). Relatively fast rate of mutation of cpSSR marker might be a major cause for such weak population differentiation. There was no identical haplotype shared between 39 population pairs of 173 pair-wise population pairs. Estimation of genetic distance among 19 populations on the basis of population pairs was also impossible, that might be resulted from restricted migration among 19 populations. Considering the observed distribution patterns of cpSSR variants in addition to the previous studies on I-SSR variants, informations on the present geographic location and genetic status of populations should be considered together for effective sustainable management of the genetic resources of Japanese red pine in Korea.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2018.11a
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• pp.92-94
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• 2018

항로표지 관리운영시스템은 항로표지 상태정보 및 해양기상정보의 감시제어 및 정보 제공 등을 위하여 지방청별로 설치 시기 및 용역사에 따라 다양하게 개발되어 운영하고 있다. 항로표지 관리운영시스템을 도입한 지방청들은 표준화되지 않은 관리운영 S/W 및 데이터 베이스를 현장 상황에 맞게 변경하여 각 지방청별로 관리운영 S/W의 유지보수 예산의 중복투자 및 관리자의 관리 운영측면에서 어려움이 있다. IALA(국제항로표지협회)는 해사 안전 분야 데이터 교환 국제표준인 S-100에 따른 항로표지 정보 표준인 S-201 규격의 개발이 진행중이다. 이에 e-Navigation 도입을 대비하여 고도화된 항로표지 정보의 체계적이고 원활한 제공을 위해 S-201을 적용한 일원화된 항로표지 관리운영시스템의 표준 S/W 개발이 필요하다. 본 연구에서는 S-201을 적용한 DB 구조를 설계하였으며, 사용자 편의성 및 시스템 관리 효율성을 개선한 새로운 항로표지 관리운영시스템 S/W를 개발하고 있다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.06a
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• pp.39-41
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• 2022

해양정보의 디지털화, e-Navigation 개념의 해양서비스 개발, 차세대 전자해도 도입, 자율운항선박 기술 개발 등 해양 분야의 주요 이슈에 대응하여 핵심 항해안전지원 시설인 항로표지 역할 변화가 요구되고 있다. 특히 국제항로표협회(IALA)의 해양자원명(MRN) 지침과 항로표지 정보교환 표준(S-201) 개발을 통해 항로표지 정보의 디지털화와 정보 서비스화를 강조하고 있으며, e-Navigation 개념의 해양 서비스 개발을 논의 중이다. 본 연구에서는 현행 항로표지 정보시스템 현황을 분석하고, 국가 연구개발 사업으로 추진 중인 스마트 항로표지 및 정보협력 시스템의 구조를 분석하였다. 또한 항로표지 기본 및 수집정보를 이용한 항로표지 정보 서비스 종류를 식별하고 서비스 운영개념을 제안하였다. 본 연구에서 제안하는 항로표지 정보 서비스 운영개념에 따라 항로표지 정보 서비스 센터 구축 및 국제 정보표준 개발 활동이 추진되어야 할 것으로 사료 된다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2022.11a
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• pp.313-314
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• 2022

항로표지법은 항로표지를 설치하고 합리적이고 효율적으로 관리하여 해상교통의 안전을 도모하고, 선박 운항의 능률을 향상시키는데 이바지하는 것을 목적하며, 항로표지의 정의와 설치·관리 및 보호, 장비·용품 등의 연구·개발 및 검사 등을 법으로 규정하고 있다. 항로표지는 해상교통의 중요한 인프라로 미래 해상환경 패러다임(MASS, AI Port, Digiral twin 등)에 대응이 가능한 스마트 항로표지로 전향하는 길목에 있다. 스마트 항로표지는 자가 고장진단 및 전원유지, 다중통신 기술 인터페이스를 제공하는 등 다목적 해양플랫폼, 전원관리시스템, 해양용다중통신 플랫폼 등을 국가 R&D를 통해 개발하고 있으며, 국내 해상 실증을 통한 글로벌 표준 제정 등의 과정을 거쳐 최종적으로는 기술이전을 통한 국내 관련 업체들의 해외진출까지를 목표로 하고 있어 기존 항로표지법을 개발될 스마트 항로표지 적용 및 활용·확산을 위한 개선 방안 마련이 필요하다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2014.10a
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• pp.347-348
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• 2014

In this paper, we describe the methods for quantitative evaluation of placement of aids to navigations (AtoN). AtoN is the additive devices for navigation that inform navigators using human vision and guide navigators to safe watercourse. Therefore the effectiveness of placement of AtoN can be quantified according to the grade of visibile recognition to navigators. In order words, the placement of AtoN can be quantified in the sense of visibility. In this study, we describe the methods for quantifying the visibility about the placement of AtoN. Using AtoN simulator which has been developed by Korea Research Institute of Ships and Ocean Engineering (KRISO), the quantitative method is investigated for the pre-installed placement of AtoNs.

• Tuberculosis and Respiratory Diseases
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• v.42 no.3
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• pp.322-331
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• 1995

Introduction: This study was performed to evaluate the diagnostic usefulness of simultaneous determination of 3 tumor markers {serum carcinoembryonic antigen(CEA), squamous cell carcinoma antigen (SCC Ag) and neuron specific enolase(NSE)} in lung cancer patients. Method: In 113 patients with primary lung cancer(70 with squamous cell carcinoma, 30 with adenocarcinoma, 13 with small cell carcinoma) and 103 patients with benign lung diseases, serum CEA and NSE were measured by enzyme immunoassay, and SCC Ag was measured by microparticle enzyme immunoassay. Results: 1) The mean serum levels of 3 tumor markers were significantly higher in lung cancer groups than benign lung disease groups respectively(p=0.001). 2) In squamous cell carcinoma, the SCC Ag was elevated in 67%, in adenocarcinoma CEA was elevated in 77% and in small cell carcinoma NSE was elevated in 77%, but there were no significant differences according to the stage of each cancer cell types. 3) CEA was the most sensitive marker, but nonspecific to cancer types. SCC Ag was less sensitive than other markers, but more specific toward squamous cell carcinoma, and NSE was more specific to primary lung cancer. 4) As the number of positive tumor markers was increased, the relative possibility of lung cancer was also increased. If two markers were positive, it increased to 77%, and if three markers were positive it increased to 90%. Conclusion: The simultaneous measurement of serum CEA, SCC Ag and NSE would provide additional information for the diagnosis of lung cancer.