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Immunohistochemical Staining

  • 박창수
    • Proceedings of the Korean Society of Veterinary Pathology Conference
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    • 2002.11a
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    • pp.38-50
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    • 2002
  • 질병의 정확한 진단과 적절한 치료 그리고 이에 대한 연구를 위해서는 병소에서 채취한 검체의 형태학적 소견을 관찰하는 것이 중요하다. 이러한 형태학적 변화를 관찰하기 위해서, 연구자들은 생검 조직으로 제작한 헤마톡실린-에오신 염색표본을 이용하고 있으며, 필요한 경우에는 세포의 미세구조를 관찰하기 위하여 전자현미경 검색을 시행하고 있다. 그러나, 각종질환은 점차 세분화되고, 새로운 진단기준이 소개되고 있으며, 종양의 진단에 도움을 주는 종양표지자, 그리고, 예후를 판정할 수 있는 예후인자들이 알려지고 있어서, 통상적인 헤마톡실린-에오신 염색표본이나 전자현미경 검색만으로는 이러한 종합적인 정보를 얻기가 어렵다. (중략)

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이달의 과학자 - 서울대의대 핵의학교실 정준기교수

  • Korean Federation of Science and Technology Societies
    • The Science & Technology
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    • v.31 no.1 s.344
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    • pp.82-83
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    • 1998
  • 87년 서울대에서 박사학위를 받은 후 그 이듬해 미국 NIH에서 방사성동위원소 표지 단일클론항체의 영상이용에 관한 연구를 하고 귀국한 서울대의대 정준기교수는 우리나라 핵의학의 정착과 발전을 위해 땀을 흘리고 있다. 서울대 암연구센터 제2연구부장을 맡고 있는 정교수는 자체 개발한 항체를 이용한 새로운 골수영상진단법으로 암의 전이 등을 진단분석하는 연구로 큰 성과를 거두고 있다. 정교수는 내년부터 의대 3학년과정에 개설된 핵의학과목을 강의하게 되어 그 준비에 바쁘다.

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법전을 통해 본 조선시대 항로표지 정책 연구

  • 김송이;문범식
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2021.11a
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    • pp.1-2
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    • 2021
  • 조선시대는 해양활동이 위축된 시대로, 대부분의 항해는 연안항해였다. 연안항해는 암초, 저수심, 좁은항로 등으로 항해자에게 해양사고의 부담을 가중시켰다. 조선시대 해양사고는 조선왕조실록 등에 209건이 기록되어 있는 것처럼 지속적으로 발생했다. 따라서 국가경제와 직결되는 조운선의 해양사고 예방은 무엇보다 중요했다. 조선정부는 조운선의 해양사고 예방을 위해 표목을 암초, 서초 등에 설치하도록 대전속록, 속대전 등에 법제화하였다.

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Expression of Hypoxia-inducible Factor-$1{\alpha}$ in Non-small Cell Lung Cancer: Relationship to Prognosis and Tumor Biomarkers (비소세포 폐암에서 HIF-$1{\alpha}$의 발현: 예후 및 종양표지자와의 관련성)

  • Cho, Sung-Rae;Byun, Joung-Hun;Kim, Jong-In;Lee, Bong-Geun;Chun, Bong-Kwon
    • Journal of Chest Surgery
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    • v.39 no.11 s.268
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    • pp.828-837
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    • 2006
  • Background: Tissue hypoxia is characteristic of many human malignant neoplasm, and hypoxia inducible factor-1(HIF-1) plays a pivotal role in essential adaptive response to hypoxia, and activates a signal pathway for the expression of the hypoxia-regulated genes, resulting in increasing $O_2$ delivery or facilitating metabolic adaptation to hypoxia. Increased level of HIF-$1{\alpha}$ has been reported in many human malignancies, but in non-small cell lung carcinoma the influence of HIF-$1{\alpha}$ on tumor biology, including neovascularization, is not still defined. In present study the relationship of HIF-$1{\alpha}$ expression on angiogenetic factors, relationship between the tumor proliferation and HIF-$1{\alpha}$ expression, interaction of HIF-$1{\alpha}$ expression and p53, and relationship between HIF-$1{\alpha}$ expression and clinico-pathological prognostic parameters were investigated. Material and Method: Archival tissue blocks recruited in this study were retrieved from fifty-nine patients with primary non-small cell lung carcinoma, who underwent pneumonectomy or lobectomy from 1997 to 1999. HIF-$1{\alpha}$, VEGF(vascular endothelial growth factor), and p53 protein expression and Ki-67 labeling index in tumor tissues were evaluated, using a standard avidin-biotin-peroxidase complex(ABC) immunohistochemistry. Relationship between the HIF-$1{\alpha}$ expression and VEGF, p53 overexpression and correlation between the HIF-$1{\alpha}$ expresseion and Ki-67 index were analyzed. Clinico-pathologic prognostic parameters were also analyzed. Result: HIF-$1{\alpha}$ expression in cancer cells was found in 24 of 59 cases of non-small cell lung carcinoma(40.7%). High HIF-$1{\alpha}$ expression was significantly associated with several pathological parameters, such as pathological TMN stage(p=0.004), pT stage(p=0.020), pN stage (p=0.029), and lymphovascular invasion(p=0.019). High HIF-$1{\alpha}$ expression was also significantly associated with VEGF immunoreactivity(p<0.001), and aberrant p53 expression(p=0.040). but was marginally associated with Ki-67 labeling index(p=0.092). The overall 5-year survival rate was 42.3%. The survival curve of patients with a high HIF-$1{\alpha}$ expression was worse than that of patients with low-expression(p=0.002). High HIF-$1{\alpha}$ expression was independent unfavorable factors with a marginal significance in multivariate analysis performed by Cox regression. Conclusion: It is suggested that high HIF-$1{\alpha}$ expression may be associated with intratumoral neovascularization possibly through HIF-VEGF pathway, and high HIF-$1{\alpha}$ expression could be associated with lymph node metastasis and post operative poor prognosis in patients with non-small cell lung carcinoma.

Production Date and Patrons of Korean Treasure #978: Transcription of the Avatamsaka Sutra (Zhou Version) in Gold on White Paper (보물 제978호 <백지금니대방광불화엄경(白紙金泥大方廣佛華嚴經) 권(卷)29>의 조성 연대 및 발원자 고찰)

  • Won, Seunghyun
    • MISULJARYO - National Museum of Korea Art Journal
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    • v.98
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    • pp.78-103
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    • 2020
  • Transcribed Buddhist sutras generally consist of a frontispiece illustration, sutra illustrations, and sutra text, although some parts may be lost over time. Most transcribed sutras originally include an official record of the transcription (saseonggi) at either the beginning or end of the volume, which document various details of the production, including who commissioned the sutra and when it was transcribed. If such records are unavailable or difficult to decipher, the date of the sutra can only be estimated by comparison to other works with known production dates. This is the case with Korean Treasure #978, the "Transcription of the Avatamsaka Sutra (Zhou Version) in Gold on White Paper" (hereinafter, "Avatamsaka Sutra, Volume 29"), which does not contain any details of its production. Based on formal comparisons, the volume has been estimated to date from the early Joseon period. Important criteria for estimating the production date include the type of calligraphy script and the overall expression of the sutra illustrations. However, these features are missing from some early Joseon sutras, making it difficult to definitively assert which characteristics are representative of the period. Also, transcribed sutras from the late Goryeo period (after 1350) and early Joseon period are often very similar in terms of the expression of the frontispiece illustrations and sutra illustrations. From the late Goryeo period through the early Joseon period, the illustrations of transcribed sutras, which had previously been relatively detailed and realistic, gradually became more formalized and stylized. Significantly, Avatamsaka Sutra, Volume 29 includes illustrations showing both styles of expression (i.e., realistic and formalized). Moreover, the hemp leaf design on the frontispiece and the border around the sutra illustrations are unique features that have never been seen on any other transcribed sutras. Notably, however, Avatamsaka Sutra in Gold on White Paper, Volume 26 (hereinafter, "Avatamsaka Sutra, Volume 26"), which has not yet been introduced in academic research, is complete with frontispiece, sutra illustrations, and sutra text. This sutra is identical to Avatamsaka Sutra, Volume 29 in size, composition, and details, and is thus estimated to have been produced at the same time and by the same patrons. According to the record at the end of the volume, Avatamsaka Sutra, Volume 26 was commissioned in 1348 by Gi Cheol (d. 1365), which corresponds to the estimated date of Avatamsaka Sutra, Volume 29 derived by formal comparison. Based on this new information, Avatamsaka Sutra, Volume 29 was likely produced in the late Goryeo period rather than the early Joseon period, as has previously been presumed. The new study of Avatamsaka Sutra, Volume 26 also seems to confirm that both sutras were transcribed by highly skilled artisans in 1348 of the late Goryeo period, a transitional phase in the expression of sutra illustrations.

A Comparative Analysis on Installation Locations of Crosswalk Markings at Signalized Intersections (교차로 내 횡단보도 설치 위치에 관한 비교 분석 연구)

  • 이호원;장덕명
    • Proceedings of the KOR-KST Conference
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    • 1998.10b
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    • pp.195-195
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    • 1998
  • 보행자 사고는 총 교통사고의 32.9%(사망 5,070명, 부상 87,943명)을 차지하고 있으며, 그 중 횡단보도 사고는 13.0%(사망 542명, 부상 10,056명)로 대책이 시급하다. 교차로 내에서 횡단보도의 위치가 부적절하면, 보행자 안전과 차량 교통소통에 악영향을 준다. 또한 우리 나라에선 명확한 기준이 없어 횡단보도 노면표지 설치 및 유지 관리에 어려움을 겪고 있는 실정이다. 현재 교차로에서 횡단보도의 위치는 다음과 같이 3종류로 구분할 수 있다. 1) 차량 정지선이 횡단보도 전방에 설치되어 진행 차량이 횡단보도를 통과하여 대기할 수 있는 공간이 있는 경우, 2) 횡단보도가 측면차량 진행방향의 연석선상(가각선의 끝 지점)에 근접하여 설치된 경우, 3) 횡단보도가 측면 좌·우회전 차량1대가 대기할 수 있는 공간의 약 4∼5m 후방(가각선의 시작지점)에 설치된 경우로 구분할 수 있다. 따라서 본 연구에서는 차량소통 및 보행자/차량 상층에 분석하여 교차로 내 횡단보도 위치기준을 제시하는데 연구 목적이 있다. 본 연구를 통한 기대효과를 살펴보면 다음과 같다, 첫째 횡단보도 내 보행자 관련 교통사고를 예방할 수 있으며, 둘째 교차로에서 보행자 및 차량소통을 원활히 할 수 있고, 셋째 횡단보도 설치 및 관리를 효율적으로 할 수 있을 것으로 판단된다.

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Very Small Putative Stem Cells Detected in Human Endometrium (인간 자궁내막에서 발견되는 극소형 추정줄기세포)

  • Choi, Jong-Ryeol;Joo, Jong-Kil;Jun, Eun-Sook;Ko, Kyoung-Rae;Lee, Hong-Gu;Lee, Kyu-Sup;Kim, Won-Gyu
    • Clinical and Experimental Reproductive Medicine
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    • v.37 no.2
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    • pp.99-113
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    • 2010
  • Objective: It has been recently reported that very small stem cells with pluripotency are detected in murine and human. The purposes of this study are to confirm whether very small putative stem cells (VSPSCs), which have the proper characteristics of stem cells as well as the expression of stem cell markers, are detected in human endometrium. Methods: The endometrial cells of 5 women, which were obtained by endometrial biopsy, were cultured for 2 weeks and were confirmed for the expressions of alkaline phosphatase, OCT-4 and CXCR4 by immunochemistry. Subsequently VSPSCs were separated by percoll density gradient method and were cultured. Also VSPSCs and their derived cells were confirmed for the expressions of OCT-4 and CXCR4. Results: The colonies, which is composed with VSPSCs less than 3 ${\mu}m$ and the 5~15 ${\mu}m$ sized hyperchromatic round cells, were detected in the endometrium of all of women and showed the strong expressions of alkaline phosphatase, OCT-4 and CXCR4. In culture after the separation of VSPSCs by percoll, these cells showed the morphological and functional characteristics of stem cells; self-renewal, colony formation, embryoid body-like formation and differential plasticity. VSPSCs formed gradually the 5~15 ${\mu}m$ sized hyperchromatic round cells and the 10~20 ${\mu}m$ sized sphere-shaped cells by cell-to-cell aggregation or cell fusion. Then these cells differentiated the various cells including fibroblast-like cells, nerve-like cells and endothelium-like cells. VSPSCs and their derived cells often showed the strong expressions of OCT-4 and CXCR4. Conclusion: VSPSCs less than 3 ${\mu}m$ and their derived cells are detected in human endometrium and these cells have the proper characteristics of stem cells and the expressions of stem cell markers as alkaline phosphatase, OCT-4 and CXCR4.

Consideration of the Effect according to Variation of Material and Respiration in Cone-Beam CT (Cone-Beam CT에서 물질 및 호흡 변화가 영상에 미치는 영향에 대한 고찰)

  • Na, Jun-Young;Kim, Jung-Mi;Kim, Dae-Sup;Kang, Tae-Young;Baek, Geum-Mun;Kwon, Gyeong-Tae
    • The Journal of Korean Society for Radiation Therapy
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    • v.24 no.1
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    • pp.15-21
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    • 2012
  • Purpose: Image Guided Radiation Therapy (IGRT) has been carried out using On-Board Imager system (OBI) in Asan Medical Center. For this reason, This study was to analyze and evaluate the impact on Cone-Beam CT according to variation of material and respiration. Materials and Methods: This study was to acquire and analyze Cone-Beam CT three times for two material: Cylider acryl (lung equvalent material, diameter 3 cm), Fiducial Marker (using clinic) under Motion Phantom able to adjust respiration pattern randomly was varying period, amplitude and baseline vis-a-vis reference respiration pattern. Results: First, According to a kind of material, when being showed 100% in the acryl and 120% in the Fiducial Marker under the condition of same movement of the motion phantom. Second, According to the respiratory alteration, when being showed 1.13 in the baseline shift 1.8 mm and 1.27 in the baseline shift 3.3 mm for acryl. when being showed 1.01 in 1 sec of period and 1.045 in 2.5 sec of period for acryl. When being showed 0.86 in 0.7 times the standard of amplitude and 1.43 in 1.7 times the standard of amplitude for acryl. when being showed 1.18 in the baseline shift 1.8 mm and 1.34 in the baseline shift 3.3 mm for Fiducial Marker. when being showed 1.0 in 1 sec of period and 1.0 in 2.5 sec of period for Fiducial Marker. When being showed 0.99 in 0.7 times the standard of amplitude and 1.66 in 1.7 times the standard of amplitude for Fiducial Marker. Conclusion: The effect of image size of CBCT was 20% in the case of Fiducial marker. The impact of changes in breathing pattern was minimum 13% - maximum 43% for Arcyl, min. 18% - max. 66% for Fiducial marker. This difference makes serious uncertainty. So, Must be stabilized breathing of patient before acquiring CBCT. also must be monitored breathing of patient in the middle of acquire. If you observe considerable change of breathing when acquiring CBCT. After Image Guided, must be need to check treatment site using fluoroscopy. If a change is too big, re-acquiring CBCT.

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Paradigm Shift in the Study of Treatment Resistant Depression (치료저항성 우울증의 연구에서 패러다임의 전환)

  • Kim, Yong-Ku
    • Korean Journal of Biological Psychiatry
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    • v.23 no.2
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    • pp.37-40
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    • 2016
  • Treatment-resistant depression (TRD) is a major public health problem. It is estimated that about 30% of patients with major depressive disorder do not show substantial clinical improvement to somatic or psychosocial treatment. Most of studies for TRD have focused on the subjects already known as TRD. Patients with unipolar depressive episodes that do not respond satisfactorily to numerous sequential treatment regimens were included in the TRD studies. Such post hoc experimental design can be regarded only as consequences of having TRD, rather than as causal risk factors for it. Although informative, data derived from such studies often do not allow a distinction to be made between cause and effect. So, we should shift paradigm toward examining the risk for developing TRD in untreated depressed patients. To deal with this problem, untreated depressed patients should be enrolled in the study to identify biological markers for treatment resistance. The peripheral or central biological markers should be explored before starting treatment. Subsequent systematic administration of treatments with appropriate monitoring in the subjects can determine the risk for developing treatment resistance in untreated individuals. Such information could give a cue to improve the initial diagnosis and provide more effective treatment for TRD.

The Diagnostic Utility of Mesothelial Markers in Distinguishing between Reactive Mesothelial Cell and Adenocarcinoma Cells in Serous Effusions with Cytospin Preparation (장액성 삼출액의 도말 표본에서 반응성 중피세포와 샘암종 세포의 감별에 중피세포 표지자의 유용성)

  • Kang, Mi-Sun;Choi, Soo-Im
    • The Korean Journal of Cytopathology
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    • v.17 no.2
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    • pp.108-115
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    • 2006
  • Evaluation of serous effusions can include immunocytochemical stains that differentiate reactive mesothelial cell from adenocarcinoma cell. Among several positive mesothelial cell markers, we used desmin, CK5/6, WT1 and calretinin all known to have high sensitivity and specificity as selective mesothelial cell markers. We studied smears obtained with cytospin from 15 malignant and eight benign effusions. The mesothelial cells were positively stained by desmin, CK5/6, WT1 and calretinin in 60.9%, 29.1%, 26.7% and 56.5%, respectively among 8 benign and 15 malignant effusions; the adenocarcinoma cells were positively stained 6.7%, 13.3%, 1.0% and 0.0%, respectively among 15 malignant effusions. The percentage of positively stained mesothelial cells were somewhat lower for all antibodies compared to the results of previous studies. This was likely due to the differences in preparation methods and fixatives among studies. In conclusion, the use of desmin and calretinin were more valuable than CK5/6 and WT1 for distinguishing between reactive mesothelial cell and adenocarcinoma cells in serous effusion; however, choice of the proper preparation methods and fixatives are also important