• Applied Microscopy
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• v.25 no.2
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• pp.53-64
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• 1995

This study was designed to investigate the morphological alterations of zonula occludens, macula adherences and gap junctions between the hepatocytes in the fasting conditions. Animals (Sprague Dawley, $250{\sim}280g$) were divided into two groups: normal and fasting. The latter were fasted for eight days prior to sampling. Liver tissues were sectioned and replicated after freeze fracturing for the transmission electron microscopy. In the normal rat liver, the interhepatocellular space at the area of some zonula occludens appeared to be widened in thin sections. On the freeze fracture replicas., the zonula occludens appeared as an anastomosing network of $2{\sim}4$ strands or grooves on P or E faces. Free ends and fragments of the strands were observed. In the rat fasted for eight days, the hepatocytes were diminished in size and the organelles were decreased in number and size. The intercellular space was wide at many areas of zonula occludens in thin section. On the freeze fracture replicas, the zonula occludens showed diminution or disappearence of anastomosing network of strands or grooves. Free ends and small fragments of the strands or grooves were frequently encountered. The macula adherens was markedly increased in number in thin sections, although they could not be found on the freeze fracture replicas. The gap junctions were increased in number in thin sections. Small aggregations of the intramembranous particles appeared with larger ones on the freeze fracture replica. The evidences may suggest the followings: (1) The disassembly of zonula occludens in the fasting states is led from the diminished mechanical stress on the luminal surface of bile canaliculus with the impaired secretion of bile components from the hepatocytes. (2) The increase of macula adherens is necessary to maintain the liver parenchyma integrity in the fasting state which leads the hepatocyte to be diminished and finally the intercellular space to be separated. (3) The rise in both number and size of gap junctions is owing to the need of increasing intercellular communication between the hepatocytes during the fasting. (4) The alteration of zonula occludens is easily led by the physiological condition of hepatocytes even in the normal ones.

• The korean journal of orthodontics
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• v.36 no.2 s.115
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• pp.103-113
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• 2006

Most elderly women experience a decrease in their bone density due to a deficiency of calcium intake, ovariectomy, or menopause. This study evaluated the usability of the microscrew as a skeletal anchorage system in these orthodontic treatment cases, using rats as a research group. The 4 month old sprague-dawley species rats were divided into two groups, the OS (Ovariectomy Screw), and the SS (Sham operation Screw) group. In both the OS and SS groups, microscrews were implanted into the palatal bone between the upper molar teeth and two upper incisors were retracted using NETE coil spring with 75 g of force. After 3days, the again after 7 days, 7 rats in each group were sacrificed. Three days before they were sacrificed, Alizarin red S was intraperitoneally injected, and their maxillary bone, tibia and blood from their hearts were taken. The components of the extracted blood were biochemically analyzed and non-decalcified grinding resin sections for maxillary bone and tibia were made. The sections were examined with a polarization microscope, and fluorescent microscope. Smaller concentrations of Ca and P, the inorganic substances closely related to bone density, were found in the extracted blood of the OS group. Both OS and SS groups showed a possibility of bone remodeling with a high concentration of ALP after 7 days. An increase in bone density on the tension and compression sides of the microscrew and the tension side of the tooth for both OS and SS groups was confirmed with a polarization microscope. However, the bone density of the pressure side of the tooth and apical side was decreased. More deposits of Alizarin red S in the bone after 7 days rather than 3 days seen with a fluorescent microscope suggested the existence of new bone formation.

• Journal of Korean Society of Forest Science
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• v.68 no.1
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• pp.60-68
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• 1985

To study the comparison of the aboveground biomass of Robinia pseudoacacia L. of 8-, 13- and 20-year-old plantations, the experimental plot of $100m^2$ in size located in Youngin-gun were selected. Seven sample trees at different stand ages selected taking account of DBH distribution were felled and the diagram of oven-dry weight distribution of stem, branch and leaf for each 1m segment was constructed. The dry weight of each part of plots was estimated by the method of basal area ratio. If the estimations are extended to a hectare area stand, it contains 36.72, 69.28 and 118.67 tons of the aboveground standing crops in 8-, 13- and 20- year-old stand respectively. The net production was estimated to be as much as 12.56, 13.23 and 16.78 tons per hectare per year and the net assimilation ratio 3.66, 4.13 and 2.50 kg/kg/yr in 8-, 13- and 20-year-old stand respectively. And the efficiency of leaves to produce stem was 1.69, 2.10 and 0.96 kg/kg/yr in same order.

• The Journal of the Korean Society for Microbiology
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• v.7 no.1
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• pp.29-41
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• 1972

To grow Myocbacterium leprae in cultured mouse peritoneal macrophages, studies were made with trypsin-purified Myco. laprae on 1) the dynamics of infection of mouse peritonal macrophages in vivo with Myco. leprae by intraperitoneal inoculation, 2) growth experiment of Myco. leprae in cultured mouse peritoneal macrophages by in vivo infection and in vitro cultivation and 3) the observation of pathological changes in spleens of mice induced by intraperitoneal inoculation of Myco. leprae. Results are summarized as follows; 1. Continuing and significant decreases were observed in the numbers of both acid-fast bacilli in cultured macrophage and of macrophages harboring.acid-fast bacilli by the length of inter vats between the time of intraperitoneal inoculation of Myco. leprae and the time of initiation of macrophage culture. 2. No evidence of multiplication of Myco. leprae in the peritoneal macrophages in vivo was found up to 5 months after intraperitoneal inoculation. 3. With cultures of macrophages made 24 hours and 1 week after intraperitoneal inoculation of Myco. leprae and maintained in vitro up to 2 to 3 months, microscopic examination of the stained preparations of cultured macrophages indicated that an apparent increase in the number of acid-fast bacilli in the macrophages did occur. 4. Quantitative experiment with in vivo infected-in vitro cultured macrophages revealed certain features of increase in the number of total acid-fast bacilli in the cultured macrophages 7 and 9 weeks after initiation of the cultures. 5. Pathological changes in the spleens mice inoculated with Myco. leprae were of mainly degenerative nature in the red pulp. No multiplication of Myco. leprae was observed in the spleens of mice up to 5 months after intraperitoneal inoculation.

• Tuberculosis and Respiratory Diseases
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• v.41 no.5
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• pp.462-474
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• 1994

Background : In acute lung injury, activated neutrophils play an important role in tissue damage. For neutrophils to participate in lung inflammation, chemotactic factors released from mononuclear phagocytes are needed to bring these cells to the local site of inflammation, with interleukin-8 (IL-8) being one of the most specific and important chemotactic factors for neutrophils. IL-8 also induces the expression of adhesion molecules and activates neutrophils to release various inflammatory mediators. Lipopolysaccharide(LPS) is one of the most important causes of adult respiratory distress syndrome and can cause release of many inflammatory cytokines including IL-8 leading to acute lung injury. But little is known about the regulatory mechanism of LPS-induced IL-8 gene expression in mononuclear phagocytes. Method : Human alveolar macrophages(HAM) and peripleral blood monocytes(PBMC) were isolated from healthy volunteers. Time and dose relationship of LPS-induced IL-8 mRNA expression was observed by Northern blot analysis. To evaluate the regulatory mechanism of LPS-induced IL-8 gene expression, pretreatment of actinomycin D(AD, $5{\mu}g/ml$) and cycloheximide(CHX, $5{\mu}g/ml$) was done and Northern blot analysis for IL-8 mRNA and ELISA for immunoreactive IL-8 protein in culture supernatant were performed. Results : 1) In HAM, dose and time dependent LPS-induced IL-8 mRNA expression was observed with peak mRNA level at 8 hours post-stimulation. 2) In PBMC, dose and time dependent LPS-induced IL-8 mRNA expression was also observed with peak mRNA level at 4 hours post-stimulation. 3) AD decreased expression of LPS-induced IL-8 gene expression at both mRNAand protein levels in both types of cells. 4) CHX decreased expression of LPS-induced IL-8 gene expression at protein level in both cell types but in HAM, superinduction of IL-8 mRNA was observed while decreased expression of IL-8 mRNA was observed in PBMC. Conclusion : Time and dose dependent LPS-induced IL-8 gene expression was observed in mononuclear phagocytes which is at least partly regulated pretranslationally. LPS-induced IL-8 mRNA expression in HAM needs no de novo protein synthesis and may be under the control of a labile repressor protein while de novo protein synthesis may be needed in PBMC.

• Journal of Korean Society of Forest Science
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• v.82 no.2
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• pp.188-194
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• 1993

A natural Quercus variabilis forest and a Populus alba${\times}$P. glandulosa plantation in Mt. Mohu area were studied to investigate aboveground biomass and net production. A $20m{\times}30m$ quadrat was set up in each stand, and 10 sample trees each of Quercus variabilis and Populus alba ${\times}$ P. glandulosa were cut for dimension analysis. There was little difference in accuracy among three biomass regression models of logWt=A+BlogD, $logWt=A+BlogD^2H$, and logWt=A+BlogD+ClogH, where Wt. D, and H were dry weight, DBH, and height, respectively. Aboveground total biomass of Quercus variabilis stand was 31,275kg/ha, and that of Populus alba ${\times}$ P. glandulosa was 55,581kg/ha. In both of Quercus variabilis stand and Populus alba ${\times}$ P. glandulosa stand, the proportion of each tree component to abovegound total biomass was high in order of stem wood, branches, stem bark, and leaves. Quercus variabilis stand was higher in the proportion of stem bark, branches and leaves than Populus alba ${\times}$ P. glandulosa stand, while the former was lower in that of stem wood than the latter. Aboveground total net production of Quercus variabilis stand was 4,267kg/ha/yr., and that of Populus alba ${\times}$ P. glandulosa stand was 3,903kg/ha/yr. The proportion of each tree component to aboveground total net production of Quercus variabilis stand was high in order of leaves, stem wood, branches, and stem bark. That of Populus alba ${\times}$ P. glandulosa stand was high in order of stem wood, leaves, branches, and stem bark. Net assimilation rate and efficiency of leaf to produce stem of Quercus variabilis stand were 2.121 and 0.840, respectively. Those of Populus alba ${\times}$ P. glandulosa stand were 3.376 and 2.085, respectively. Though Populus alba${\times}$P. glandulosa stand was lower in aboveground total net production than Quercus variabilis stand, the former was higher in aboveground total biomass than the latter. The reason was that Populus alba${\times}$P. glandulosa stand was higher in net production of stem wood of accumulation organs than Quercus variablis stand.

• Korean Security Journal
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• no.60
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• pp.33-61
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• 2019

It is quite difficult to actually prove the effectiveness of so-called target-hardening, one of the various strategies used to reduce crime, one of the serious problems in society recently. In particular, three to five minutes is often used as golden time for intruders to give up or stop, which is based on foreign and some indirect research cases in Korea, but there were no studies that more directly identified the average break-in operation time or the abandonment time based on the elapsed time when the shield hardware resists intruders. This study was the first of its kind in Korea to investigate and verify samples of 90 inmates of break-in burglars who were imprisoned in August 2018 by profiling the average criminal experience, education level, age, height and weight of typical Korean professional break-in thieves, and specific criminal methods, average break-in operation time, and the criteria for giving up if not breached. According to the analysis results, in the survey on the number of pre-invasion theft crimes by intruders, many of the respondents who participated in the survey were criminals of professional invasions, and by their physical characteristics, there was not much difference from ordinary adult men. Residential facilities were the highest in the world, followed by commercial and educational facilities. According to the survey on the types of facilities that committed intrusion into residential facilities, it was not safe to say that single-family housing accounted for the largest portion of single-family housing, multi-family housing, apartment high-rise (more than three stories), and apartment low-rise (more than one to three stories) among residential facilities, and that the ratio of apartment high-rise was higher than expected. Based on the average time required to break into a place for an intrusion crime, it is assumed that the psychological time worked in a place where the break-in was difficult, since the break-in was not performed while measuring the time of the break-in operation. In the case of time to give up a crime, more than half of the respondents said they would give up the crime even in less than four minutes, suggesting that a significant number of intrusive crimes can be prevented even if the facility has four minutes of intrusion resistance. This proves that most intruders will give up the break-in if the break-in resistance performance of the security facility is exercised for more than five minutes.

• Journal of Hospice and Palliative Care
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• v.15 no.1
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• pp.18-29
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• 2012

Purpose: We analyzed how clinical nurses in Korea perceive terminally ill patients' medical decision-making. Methods: The Q-methodology which analyzes the subjectivity of each item was used. We selected 34 Q-statements among those provided by each of 37 subjects and grouped them into a shape of normal distribution using a 9 point scale. The collected data were analyzed using a QUANL PC program. Results: Four types of perception toward medical decision-making were identified. Type I focuses on patient participation, and Type II emphasizes the role of health professionals. Type III is characterized by an open-minded culture toward death, and Type IV values the role of family members. Conclusion: The results of this study indicate the need for development of a multi-disciplinary curriculum medical decision-making and death for medical and nursing students.

• Radiation Oncology Journal
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• v.21 no.2
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• pp.149-157
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• 2003

Purpose: The purpose of this study was to determine if there were prognostic differences between BRCA related and BRCA non-related Korean patients with early-onset breast carcinomas. Materials and Methods: Sixty women who had developed breast cancers before the age of 40, and who were treated at the Soonchunhyang University Hospital, were studied independently of their family histories. The age range was 18 to 40 with a median of 34.5 years. Lymphocyte specimens from peripheral blood were studied for the heterozygous mutations of BRCA1 and BRCA2 using direct sequencing methods. Immunohistochemistry was peformed on the paraffin-embedded tissue blocks that were available. Results: Eleven deleterious mutations (18.3%, 6 in BRCA1 and 5 in BRCA2) and 7 missense mutations of unknown significance (11.7%), were found among the 60 patients. More than half of the mutation were novel, and were not reported in the database. Most of the BRCA-associated patients had no history of breast cancer. No treatment related failures were observed in the BRCA carriers, with the exception of one patient that had experienced a new primary tumor of the contralateral breast. The seven year relapse free survival rate were 50 and 79% In the BRCA carrier and BRCA negative patients, respectively. Although the expression of estrogen and progesterone receptors were less common, and histological features more aggressive, in the BRCA associated tumors, the outcome of the patients with BRCA mutations was not poorer than that on the patients without deleterious mutations. Conclusion.: Despite the BRCA mutation carriers having adverse prognostic features, the recurrence rate was relatively lower than that in the BRCA non-carrying Korean patients wi4h early-onset breast carcinomas. In addition, although the prevalence of the BRCA mutation in Korean patients was higher than that in white patients, the penetrance of the cancer seemed to be relatively low in Korean women carrying BRCA mutations. A large population based study of the BRCA mutation, with a long-term follow-up of the study patients will be required to confirm these results.

• Korean Journal of Ecology and Environment
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• v.49 no.1
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• pp.31-41
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• 2016

Deutzia paniculata is an endemic species to the Korean Peninsula. Despite of importance for conservation, the population structure and habitat characteristics of D. paniculata have not been determined yet. We analyzed the ecological characteristics of the species based on the literature review and field survey. Field survey was conducted on May to October 2014 during which 11 quadrats of size $15{\times}15m$ were studied in six regions. Each of the quadrats were further divided into $5{\times}5m$ small quadrats and population characteristics were recorded. The population and habitat characteristics were analyzed, including species abundance (density and coverage), demographic attributes (flowering rates and fruiting plants), vegetation (structure, species composition), light availability (transmitted light and canopy openness) and soil characteristics (temperature and humidity). We found that D. paniculata mainly distributed in Gyeongsangdo (including Taebaek in Gangwondo) along a broad elevational range of 290~959 m (mean: 493 m) above sea level. In preferred habitat the species grows within the slope range of $7^{\circ}$ and $35^{\circ}$ with the average of $16^{\circ}$. D. paniculata was generally distributed on talus deposits and low adjacent slopes. The average number of individual plants per small quadrat was 12.5 with the mean density $0.5stems\;m^{-2}$. The vegetative reproduction was frequent in D. paniculata and mean flowering rate was as low as 15%. Altogether 138 taxa were found in whole observation area with the dominant tree species mainly spring ephemerals, such as Cornus controversa (importance value: 25.5%) and Fraxinus rhynchophylla (importance value: 15.8%). Although, C. controversa usually grows on steep slopes and F. rhynchophylla mostly distributed at high-altitudes, however, both species distributed in disturbed environments and among talus deposits. Thus based on our results, we concluded that D. paniculata is a disturbance-prone species, primarily existing in habitats subjected to natural disturbances, such as floods. The species occurs less at anthropogenically disturbed sites, thus there is no apparent threat to the populations and habitat of D. paniculata.