• Title/Summary/Keyword: 포장저항성

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Mapping of RFLP Markers Linked to Bacterial Blight Resistant Genes (Xa-1, Xa-3) in Rice (벼 흰잎마름병 저항성 유전자(Xa-1, Xa-3)연관 RFLP 마커 탐색)

  • 강현중;김현순;남정권;이영태;이승엽;김석동
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.419-423
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    • 2003
  • Bacterial blight caused by Xantomonas oryzae pv. oryzae is one of the most serious diseases of rice especially in southern area of Korea. Three races, $\textrm{K}_1$, $\textrm{K}_2$ and $\textrm{K}_3$, are the most dominant species. lo improve rice breeding efficiency using marker assisted selection, some RFLP markers were surveyed for polymorphism between resistant and susceptible to $\textrm{K}_1$ and $\textrm{K}_3$. And, 127 doubled-haploid (DH) lines derived from Milyang121/HRl1650-1-4-2 and 131 DH lines derived from Milyang123/HR10624-AC5 were evaluated to bacterial blight ($\textrm{K}_1$ and $\textrm{K}_3$). Milyang121 and HR10624-AC5 have Xa-1, resistant to $\textrm{K}_1$ race, and Milyang123 has Xa-3, resistant to $\textrm{K}_1$ and $\textrm{K}_3$ race. Three markers, RZ590, RZ536 and RG303, showing polymorphism between parents and resistance gene, Xa-1 and Xa-3, were analysed in the two combinations of DH lines. The segregation pattern of resistant DH population of Milyang123/HR10624-AC5 to susceptible showed 3:1 and 1:1 in $\textrm{K}_1$ and $\textrm{K}_3$ race. In three RFLP markers, RZ590 was linked to Xa-1 on chromosome 4, and RZ536 and RG303 were linked to Xa-3 on chromosome 11. The map distance between Xa-1 and RZ590 was 3.1cM on chromosome 4, and Xa-3 and RZ536/RG303 were 7.6/16.0cM on chromosome 11, respectively. The results of RFLP mapping will be useful for the selection and pyramiding of bacterial blight resistant genes.

Monitoring for the Resistance of Botrytis cinerea Causing Gray Mold Against Mepanipyrim (Mepanipyrim에 대한 잿빛곰팡이병균의 저항성 검정)

  • Kim, Ah Hyeong;Kim, Seon Bo;Han, Kee Don;Kim, Heung Tae
    • The Korean Journal of Pesticide Science
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    • v.19 no.3
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    • pp.329-334
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    • 2015
  • With 86 isolates of Botrytis cinerea obtained from diseased fruits of tomato, strawberry and cucumber, it was conducted to detect the fungicide resistance of B. cinerea against mepanipyrim through an agar dilution method. FGA medium was used for monitoring the resistance. Among 86 isolates of B. cinerea, resistant isolates were composed by 23.3%, which was different according to regions isolating the pathogen. In accordance with isolation region, the isolation frequency of resistant isolates was as follows; that of Gyeonggi, Gyeongnam/Pusan and chungnam was 28.6%, 33.3% and 12.8%, respectively. The resistant isolates of B. cinerea showed the cross resistance between mepanipyrim and pyrimethanil. Also the pathogenicity of B. cinerea resistant to mepanipyrim was high as like that of sensitive isolates. Because of them, it should be necessary to manage the spraying system of mepanipyrim and the monitoring for the fungicide resistance.

Monitoring for the Resistance of Strobilurin Fungicide Against Botrytis cinerea Causing Gray Mold Disease (Strobilurin계 살균제에 대한 잿빛곰팡이병균 Botrytis cinerea의 저항성 검정)

  • Kim, Ah Hyeong;Kim, Seon Bo;Han, Ki Don;Kim, Heung Tae
    • The Korean Journal of Pesticide Science
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    • v.18 no.3
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    • pp.161-167
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    • 2014
  • This study was conducted to investigate the resistance of Botrytis cinerea to azoxystrobin, which belongs to strobilurin fungicides. The sensitivity of B. cinerea isolates, which were collected from infected pepper, strawberry, cucumber and tomato by a single spore isolation, to the fungicide was tested through a agar dilution method on PDA amended with fungicides and $100{\mu}g\;mL^{-1}$ of salicylhydroxamic acid (SHAM). All isolates of B. cinerea tested in this study were classified as a sensitive and a resistant group by $1.0{\mu}g\;mL^{-1}$ of $EC_{50}$ value to azoxystrobin. While the sensitive isolates accounted for 46.5% of B. cinerea population, the resistant ones did for 53.5%. According to the regions isolating B. cinerea, the highest isolation frequency was showed as 81.1% in Chungnam among the all. Among 4 host plants as pepper, strawberry, cucumber and tomato, the highest isolation frequency was obtained in strawberry, while the lowest was done in pepper. The isolate resistant to azoxystrobin showed the cross resistance to other fungicides included into strobilurins as kresoxim-methyl and trifloxystrobin. In spite of an excellent efficacy of strobilurins, it should be taken care to use them in the field, because of the high risk in the fields.

A Comparative Study on Pavement Techniques for Steel Bridge Decks (강상판형교 교면포장 공법 비교 연구)

  • Kim, Nakseok
    • Journal of the Society of Disaster Information
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    • v.8 no.2
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    • pp.100-107
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    • 2012
  • The main purpose of this research is to propose advanced bridge-decks surfacing from the examples of In-Cheon bridge with Guss asphalt and Gyul-Hyeon bridge with stone matrix asphalt(SMA) concrete. The research results presented that the Guss asphalt concrete pavement was weak when rutting was developed due to the low resistance at high temperatures. The SMA concrete pavement demonstrated good resistance to plastic deformations and was easy to maintain pavement conditions. The results mentioned above are generalized in developed counties where surfacing pavement techniques for steel bridge-decks are advanced. However, due to the lack of construction experiences in Korea, appropriate construction guidelines and organized research for the steel bridge deck pavements are necessary for more advanced steel bridge decks surfacing.

Characteristic of Progeny in Pepper Transformants (고추 유전자변형체 후대 생육특성 검정)

  • Kwon, Tae-Ryong;Lee, Moon-Jung;Harn, Jung-Sul;Shin, Dong-Hyun;Oh, Jung-Youl;Kim, Kyung-Min;Kim, Chang-Kil
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.260-264
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    • 2008
  • For the resistance test for Phytophthora blight of $T_1$ and $T_2$ transformants in pepper, Phytophthora blight fungus was inoculated to seedlings of the $T_1$ and $T_2$ transformants by concentration (density: zoospore $10^3/ml$). Occurrence rate of blight at 5days after inoculation was 4.0 % in T1-1 line and 10.0% in $T_1-2$ line, and its rate for 12 days after inoculation was 52.0% in $T_1-1$ line, 64.0% in $T_1-2$ line, respectively. Therefore, the lower occurrence rate to blight was enable to select resistant transformants in the some inoculation density (zoospore $10^3/ml$), meanwhile 'Kumtap' and 'Subicho' were 100% in highest occurrence rate to blight. For field test, in which blight was commonly occurred, of the Youngyang Pepper Experiment Station, the acquired transformant resisting to blight was similar to characteristics of domestic varieties, 'Subic ho' for fruit shape, but there are some differences in growth, days to flowering, fruit characteristics. Occurrence of blight in $T_2-1-6$, and $T_2-4-9$ lines was smaller approxmately 30% than commercial varieties, 'Kumtap', although occurrence of blight in field was showed higher difference among tested lines. In this study, we concluded that the transformants showing blight resistance selected from habitual field could be fixed at every generation, and the developed transformation system was also considered to develop transformants in pepper.

Modeling of Friction Characteristic Between Concrete Pavement Slab and Subbase (콘크리트 포장 슬래브와 보조기층 간 마찰특성 모형화)

  • Lim, Jin-Sun;Son, Suk-Chul;Liu, Ju-Ho;Jeong, Jin-Hoon
    • International Journal of Highway Engineering
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    • v.12 no.4
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    • pp.211-218
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    • 2010
  • Volume of concrete slab changes by temperature and moisture effects. At that time, tensile stress develops because the slab volume change is restrained by friction resistance between the slab and subbase, and then crack occurs occasionally. Accordingly, researchers have made efforts to figure out the friction characteristics between the slab and subbase by performing push-off tests. Lately, researches to analyze concrete pavement behavior by the friction characteristics have been performed by finite element method. In this study, The friction characteristics between the slab and subbase were investigated based on the friction test results for lean concrete, aggregate, and asphalt subase widely used in Korean concrete pavements. The energy method bilinearizing relation between nonlinear friction resistance and displacement were suggested. The friction test was modeled by 3-D finite element program, ABAQUS, and the model was verified by comparing the analyzed results to the test results. The bilinear model developed by the energy method was validated by comparing analysis results obtained by using the nonlinear and bilinear friction resistance displacement relation as input data. A typical Korean concrete pavement was modeled by ABAQUS and EverFE and analyzed results were compared to evaluate applicability of the bilinear model.

Joint Toxic Action of Bifenthrin and Prothiofos Mixture for the Control of Insectivcide-Resistant Diamondback Moth, Plutella xylostella L. (살충제 저항성 배추좀나방 방제를 위한 Bifenthrin과 Prothiofos 혼용의 연합작용)

  • 정부근;강수웅;추호렬
    • Korean journal of applied entomology
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    • v.36 no.1
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    • pp.105-110
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    • 1997
  • Mixture study of bifenthrin and prothiofos was conducted to control insecticide resistant diamondback moth (DBM), Plutella xylostella, which had shown 581, 18, 19, 11 fold resistance to fenvalerate, cypermethrin, furathiocarb, and prothiofos in Chinju strain, respectively and 38 and 9 fold resistance to fenvalerate and furathiocarb in Seosang strain, respectively. Optimal mixture ratios of bifenthrin and prothiofos was selected against Seosang strain of DBM by leaf dipping method in laboratory and by field test. Maximum co-toxicity coefficient by dipping method was shown at a ratio of 1:50 mixture of bifenthrin 1EC and prothiofos 50EC in active ingredient(a.i.) by 243.2 and then suddenly decreased. The mixed formulation from the dipping test was examined at the cabbage field of Seosang, Hamyang. Although insecticides were reduced to half, control efficacy was similar to farmer's conventional method. In addition, the mixture combination was also more effective against Chinju DBM population which had very high level of resistance to several representative insecticides. Mixture methods of insecticides will be effective countermeasures to the resistance problem of pests.

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Evaluation of Characteristic Improvement of Waste-Polyethylene Asphalt Concrete (폐폴리에틸렌 필름 재활용 아스팔트 콘크리트의 특성 분석)

  • Kim, Kwang-Woo;Li, Xing-Fan;Jeong, Seung-Ho;Lee, Soon-Jae;Lee, Gi-Ho
    • International Journal of Highway Engineering
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    • v.4 no.1 s.11
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    • pp.161-170
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    • 2002
  • This study is a fundamental research for recycling waste polyethylene film(WPF) in asphalt concrete for roadway pavement. The objective of this study is to develop technology of making waste polyethylene asphalt mixture and evaluate properties of the asphalt concrete containing WPF. Asphalt concrete for surface course of pavement was produced through an appropriate mix-design using dense-graded and gap-graded aggregates. Marshall mix design, indirect tensile strength test, wheel tracking test and tensile fatigue test were performed. Test result showed that some WPF asphalt mixtures had a high tensile property and good resistances against rutting and fatigue cracking, compared with normal asphalt mixture.

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Performance Evaluation of Asphalt Pavement Reinforced with Glass Fiber Sheet Type of Geosynthetics (유리섬유시트 형태의 토목섬유로 보강된 아스팔트 포장의 공용성 평가)

  • Cho, Sam-Deok;Lee, Dae-Young
    • Journal of the Korean Geosynthetics Society
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    • v.10 no.3
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    • pp.1-8
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    • 2011
  • This paper presents the performance evaluation of asphalt pavement reinforced with fiber sheet type of geosynthetics and observations conducted to evaluate the practical efficiencies and performance of overlay asphalt pavement reinforced with geosynthetics. In this study, performance evaluation were performed for the six section of construction site. The performance indcators of asphalt pavement reinforced with geosynthetics has been collected Automatic Road Analyzer (ARAN), Falling Weight Deflectometer (FWD) and have been analyzed for rutting, cracking ratio, falling weight and international roughness index. As a result of performance evaluations, geosynthetics reinforced asphalt pavement is sigficant effect on increasing a cracking resistance than the non-reinfroced asphalt pavement, also rutting and crak is slowly increase as incerasingly performance period.

Herbicidal Activity of New Rice Herbicide Pyrimisulfan+Mefenacet SC against Sulfonlurea-Resistant Sea Club Rush (Scirpus planiculmis) (설포닐우레아계 제초제 저항성 새섬매자기에 대한 신규 제초제 Pyrimisulfan+Mefenacet SC의 약제방제 효과)

  • Park, Min-Sik;Park, Yong-Seog;Kim, Sung-Min;Lee, Jeong-Deug
    • Korean Journal of Weed Science
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    • v.31 no.2
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    • pp.192-198
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    • 2011
  • This study was conducted to investigate the herbicidal activity against Scirpus planiculmis (SCPPL) which has been reported as resistant biotype on sulfonylurea (SU) herbicides at the west coast reclaimed area first and proliferated continuously at Hwasung and Pyeongtaek in Gyeonggi province, and some Chungnam province. In order to investigate resistance breakability against SCPPL, new rice herbicides containing tefuryltrione [p-hydrophenyl pyruvate dioxygenase (HPPD) inhibitor], pyrimisulfan and triafamone[actolactate synthethase (ALS) inhibitor] were tested. In both lab and field experiments, tefuryltrione GR, 4-HPPD inhibitor showed growth retardation with a bleaching on the young leaves of SCPPL at early time after treatment. However, it restored and regerminated with insufficient control effect. On the other hand, pyrimisulfan+mefenacet SC, triafamone+tefuryltrione GR, and ALS inhibitor showed excellent control effect on SU-resistant SCPPL with growth retardation and necrosis. In particular, pyrimisulfan+mefenacet SC showed excellent controlling effect on SU-resistant SCPPL in regional field experiments.