• Title/Summary/Keyword: 포도구균

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Profile of the Staphylococcal Exotoxin Gene and its Relation with Canine Atopic Dermatitis (포도알구균의 외독소 유전자 분석과 그 외독소가 개 아토피 피부염에 미치는 영향)

  • Nam, Eui-Hwa;Chung, Tae-Ho;Kim, Ji-Hyun;Park, Seol-Hee;Kim, Hyo-Eun;Youn, Hwa-Young;Chae, Joon-Seok;Park, Yong-Ho;Hwang, Cheol-Yong
    • Journal of Veterinary Clinics
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    • v.28 no.2
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    • pp.196-203
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    • 2011
  • Staphylococcus spp. is one of the most common bacteria isolated from the lesions of atopic dermatitis (AD) in humans, and their colonization is known to be a possible trigger factor of clinical signs. The aim of this study was to determine the prevalence of Staphylococcus spp. in canine AD (CAD), the types of exotoxins present, and their relation with the clinical severity of CAD. From 79 dogs with AD, 72 samples of Staphylococcus spp. were isolated (91.1%), and 65 (90.3%) were confirmed as Staphylococcus pseudintermedius. Concerning the profile of the exotoxin gene, 50 isolates (69.4%) contained at least one exotoxin gene, and 28 isolates (56%) were found to contain more than 2 different exotoxins. There was a significant difference in clinical severity with the presence of staphylococcal exotoxins (P=0.028), whereas no correlation was found with the presence of Staphylococcus spp. (P=0.598). The clinical severity of CAD increased only in relation to staphylococcal enterotoxin D (SED) and exfoliative toxins (P<0.05). Some clinical evaluation criteria (erythema, papule/pustule) were correlated with the presence of the exotoxin gene (P<0.05). This study showed that the high prevalence of Staphylococcus spp. and staphylococcal exotoxins in lesions from dogs with AD may be regarded as an important trigger factor for exacerbation of the clinical signs of CAD.

Screening of MRSA (Methicilline Resistant Staphylococcus Aureus) and seb Gene in Producing Strains Isolated from Food Service Environment of Elementary Schools (초등학교 급식 환경에서의 메치실린 내성 황색포도상구균(MRSA)과 seb gene의 검색)

  • 하광수;박선자;심원보;정덕화
    • Journal of Food Hygiene and Safety
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    • v.18 no.2
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    • pp.79-86
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    • 2003
  • Most of food poisoning is frequently raised from mass catering. Especially, staphylococci takes the large part of pathogenic agents which are related to the hygienic condition. Among total 98 samples, four staphylococci were isolated from food service environment such as drinking water (A), hands (D), refrigerator and apron (E) of 5 elementary school (A, B, C, D, E) in Gyeongnam Province. These isolated strains are characterized as 1 MRCNS (Methicilline Resistant Coagulase Negative Staphylococcus aureus) and 3 MSCPS (Methicilline Sensitive Coagulase positive Staphylococcus aureus). Also, production of enterotoxin B (sob gene) were examined by PCR which has known as a big problem because of their temperature resistance. Hence, PCR was performed on isolated 4 staphylococci. The all 4 isolated Staphylococcus aureus have 477 bp of seb gene. Antibiotics susceptibility test was completed on PCR detected strains. All strains were fully resistance to ampicillin and penicillin. The drinking water of A place has resistance to oxacilline, therefore this strain turned out to be MRSA (Methicilline Resistant Staphylococcus Aureus).

Isolation of Staphylococcus from Raw Milks and Their Antimicrobial Drug Susceptibility (생유중 포도구균의 분리빈도와 약제감수성)

  • Lee Ju-Hong;Lee Soon-Sun;Lee Kuk-Chun;Kang Ho-Jo
    • Journal of the korean veterinary medical association
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    • v.20 no.2
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    • pp.103-108
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    • 1984
  • A total of 359 cultures of Staphylococcus were isolated from no raw milk samples in stock farms of Gyeongnam province from December, 1981 to May, 1982. Some biochemical properties and antimicrobial drug susceptibility of the isolaes were studied with the

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Drug Resistance of Staphylococcus aureus Isolates from Raw Cow Milk (생유에서 분리된 포도구균의 약제내성)

  • Kim Jong-Myeon;Song Hee-Jong;Kwak Taik-Hoon
    • Journal of the korean veterinary medical association
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    • v.15 no.3
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    • pp.143-148
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    • 1979
  • A total of 360 Staphylococcus isolates from 26 raw cow milk sample in milk plants of Jeonju district during Octover 1977, identified by $7.5\%$ salt resistant reaction and other biological properties. Antibiotic sensitivity test was also perfor

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Differences in the Antibiotic Resistance Pattern of Staphylococcus aureus Isolated by Clinical Specimens in a University Hospital in South Korea (일개 대학병원의 임상 검체에서 분리한 포도알균 항생제 내성 경향 차이)

  • Hong, Seong-No;Kim, Joon;Sung, Hyun-Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.2
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    • pp.85-92
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    • 2018
  • Information on the prevalence of S. aureus and the current antimicrobial resistance profile is necessary in selecting the appropriate treatment of S. aureus infections in any part of the world. This study examined the frequency and antibiotic resistance list of S. aureus isolates obtained from clinical specimens at one hospital in Korea. A total of 1,746 gram positive cocci collected were identified as S. aureus. S. aureus isolates were obtained from different samples including sputum (N=565; 32.4%), endotracheal aspirate (358; 20.5%), wounds (329; 18.8%), blood (137; 7.8%), urine (67; 3.8%), and pus (59; 3.4%). All 1,545 S. aureus (100%) strains screened from sputum (565; 36.6%), endotracheal aspirate (388; 25.1%), wounds (329; 21.3%), blood (137; 8.9%), urine (67; 4.3%), and pus (59; 3.8%) were sensitive to glycopeptide (vancomycin, teicoplanin), oxazolidinone (linezolid) and stretogramin (quinupristin/dalfopristin). The prevalence of resistant S. aureus was significantly (P<0.01) lower in urine, blood, pus, wounds, and sputum than in endotracheal aspirates. As a result, there was a significant difference in the antibiotic resistance of S. aureus according to the clinical specimens.

Efficacy of Bovine Staphylococcal Mastitis Vaccine Composed of Alpha toxin, Capsular Polysaccharide and Fibronectin Binding Protein in Lactating Cows and Heifers (비유우와 처녀우에서 황색포도구균의 alpha toxin, capsular polysaccharide와 fibronectin binding protein으로 구성된 유방염 백신의 효능)

  • 한홍율;박희명
    • Journal of Veterinary Clinics
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    • v.17 no.1
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    • pp.152-158
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    • 2000
  • capsular polysaccharide(CPS), alpha toxin과 재조합 fibronectin binding protein (FnBP)으로 구성된 유방염 백신을 개발하여 야외효능실험을 수행하였다. 이 subunit 백신은 비유우 23두와 처녀우 20두를 대상으로 10개월동안 각각 수행되었다. 비유우는 매 3주간격으로 2회 백신접종을 하였으며 대조군은 PBS를 상유방림조절 주위에 피하주사하였다. 처녀우를 대상으로 한 실험에서는 분만 8주전부터 시작하여 매 3주간격으로 2회 비유우 백신접종부위와 동일하게 접종하였다. 접종후 비유우에서는 황색포도상구균에 의한 총유선내 감염율이 대조균에 비해 유의성있게 감소하였으며 (p<0.001) 처녀우에서도 총유선내 감염이 백신 접종균(1.6%)이 대조군(11.7%)에 비해 다음 비유기동안 유의성있게 낮았다. (p<0.001). 비유기동안 백신접종한 젖소의 체세포수는 변화가 없었으며 처녀우에서는 추가접종후 백신접종군에서 대조군의 체세포수에 비해 낮았지만 통계학적으로 유의성은 없었다(p>0.05). 본 실험결과 황색포도구균에 대한 유방염 아단위 백신은 비유우와 처녀우에서 체세포수를 증가시키지 않고 총 유선내 감염율을 낮추어주었다. 하지만 본 실험은 1군데의 목장을 대상으로 하였기 때문에 향후 대규모 목장을 대상으로 하는 유방염 백신의 야외효능실험이 심도있게 이루어져야 한다고 생각된다.

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