• Title/Summary/Keyword: 평활

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The Viability & Vascularization of the Cryopreserved Rat Tracheal Allografts with Omental Implantation (초냉동 보관된 백서의 동종 기관 이식편의 대망 내 이식에 따른 조직 생육성 및 혈관 형성)

  • 김용희;김동관;김규래;박승일
    • Journal of Chest Surgery
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    • v.37 no.8
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    • pp.623-631
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    • 2004
  • Background: Using the neovascularizing properties of the omentum, we studied the viability and vascularity of the cryopreserved rat tracheal allografts with omental implantation. Material and Method: The cryopreserved tracheal allografts of eight-week old male Sprague Dawley rats were implanted into the omentum. The rats were divided into the four groups according to the duration of cryopreservation and of omental implantation. We examined the tracheal allografts histologically for viability of cartilages, inflammation and fibrosis of smooth muscle and connective tissue, and degree of vascularity. Result: The degree of inflammation in the smooth muscle and the connective tissue of the tracheal allografts was not statistically related to neither the duration of cryopreservation or of omental implantation. The tracheal cartilages of the tracheal allografts were found to be severely calcified in all cases. Significant difference in vascularity was found between the groups I and II (p < 0.05). And a sufficient vascularity in the intercartilaginous space was observed in the mid portion of the tracheal allografts as well as both ends. Conclusion: In conclusion, the omental implantation for 2 weeks could establish a sufficient vascularity in the intercartilaginous spaces for maintaining the viability of the tracheal allografts. This study might provide a possibility of the sequential tracheal allotransplantation after omental implantation.

반응성 고주파 마그네트론 스퍼터링 방법에 의한 AlN 압전 박막 증착 및 특성에 관한 연구

  • 황지현;권명회;김형택
    • Proceedings of the Korean Vacuum Society Conference
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    • 2000.02a
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    • pp.89-89
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    • 2000
  • AlN 박막은 Al과 N원자의 부분적 이온결합 특성을 가진 공유결합을 한 육방정계의 wurtzite 경정구조의 화합물 반도체로서, III-V족 반도체 중 가장 큰 에너지 갭(6.2 eV), 결정 구조적 이방성, 화학 양론적 결합구조, 높은 탄성종과 전달속도(약 10$\times$106 m/s)와 높은 열전도도, 고온 안정성, 가시광성.적외선 영역에서의 좋은 투과성과 높은 굴절률, 상온 대기압에서의 유일하게 안정적인 특성을 가지고 있어, 절연재료, 내열재료, 저주파 영역 센서의 압전 트랜스듀서, 광전소자, 탄성파 소자 및 내환경 소자, MIS소자 등으로 주목받고 있다. 본 연구에서는 BAW 공진기의 활용을 목적으로 반응성 마그네트론 스퍼터링 방법으로 AIN 압전박막을 제작하여, 증착 조건-질소 농도, 고주파 출력, 전체 스퍼터링 압력, 기판 온도-에 대한 박막의 특성을 조사하였다. AlN 박막의 c축 우선 방위 결정성 및 낮은 투과성, 적당한 굴절률의 특성이 BAW 공진기의 활용을 위한 요건이므로, 각각의 증착 조건하에 제작된 박막은 XRD의 $\theta$/2$\theta$ 스캔 회절상에 의한 결정성의 분석과 우선 성장 결정면의 rocking curve 및 XRD로 측정한 FWHM과 표준 편차로 결정성의 배열성과 소자 응용가능성을 조사하였다. 박막의 표면.단면 미세 구조 및 평활도는 SEM으로 관찰하였으며, Al-N 결합 상태는 XPS와 FT-IR로 분석 조사하였다. 제작된 AlN 박막의 결정성 분석 결과, c축 우선 방위 성장을 위한 스퍼터링 압력에 대한 임계 질소 농도와 임계 스퍼터링 압력이 관찰되었다. 전체 스퍼터링 압력이 6~8 mTorr의 범위에서 나타난 최소 임계질소 농도는 10%, 최대 임계 질소 농도는 60%이며, 4 m Torr 이하 10 m Torr 이상의 전체 스퍼터링 압력에서 박막의 우선 방위성장이 제재된다. 이는 AlN 박막이 형성에 관여하는 질소 이온 양의 충분한 형성에 필요로 하는 질소 가스의 유입량에 따른 것으로 판단된다. AlN 박막의 c축 결정면인 (002) 결정면의 성장을 유도하며 다른 방향으로의 성장을 제어하여 소자 활용에 유용한 박막을 제작하기 위한 고주파 출력은 300W 정도가 적당하며, 기판을 가열하지 않았을 때 낮은 투과도를 나타낸다. 본 연구에 의한 BAW 공진기 활용을 위한 AlN 압전박막의 제작을 위한 최적 증착 조건은 기판의 가열 없이 6~8 mTorr의 전체 스퍼터링 압력에 20~25%의 질소종도, 300W의 고주파 출력이다. 최적 조건에서의 AlN 박막은 약 0.19$^{\circ}$의 FWHM과 약 0.08$^{\circ}$의 표준편차를 가지며, 균일하고 조밀한 표면 미세구조와 주상정 구조의 측면구조, 파장에 대한 약 2.0의 굴절률, 낮은 투과도와 화학 양론적 구조를 가지는 우수한 박막이 형성되었다.

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A comparative study for reconstructing a high-quality NDVI time series data derived from MODIS surface reflectance (MODIS 지표 분광반사도 자료를 이용한 고품질 NDVI 시계열 자료 생성의 기법 비교 연구)

  • Lee, Jihye;Kang, Sinkyu;Jang, Keunchang;Hong, Suk Young
    • Korean Journal of Remote Sensing
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    • v.31 no.2
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    • pp.149-160
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    • 2015
  • A comparative study was conducted for alternative consecutive procedures of detection of cloud-contaminated pixels and gap-filling and smoothing of time-series data to produce high-quality gapless satellite vegetation index (i.e. Normalized Difference Vegetation Index, NDVI). Performances of five alternative methods for detecting cloud contaminations were tested with ground-observed cloudiness data. The data gap was filled with a simple linear interpolation and then, it was applied two alternative smoothing methods (i.e. Savitzky-Golay and Wavelet transform). Moderate resolution imaging spectroradiometer (MODIS) data were used in this study. Among the alternative cloud detection methods, a criterion of MODIS Band 3 reflectance over 10% showed best accuracy with an agreement rate of 85%, which was followed by criteria of MODIS Quality assessment (82%) and Band 3 reflectance over 20% (81%), respectively. In smoothing process, the Savitzky-Golay filter was better performed to retain original NDVI patterns than the wavelet transform. This study demonstrated an operational framework of gapdetection, filling, and smoothing to produce high-quality satellite vegetation index.

Short-term Traffic States Prediction Using k-Nearest Neighbor Algorithm: Focused on Urban Expressway in Seoul (k-NN 알고리즘을 활용한 단기 교통상황 예측: 서울시 도시고속도로 사례)

  • KIM, Hyungjoo;PARK, Shin Hyoung;JANG, Kitae
    • Journal of Korean Society of Transportation
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    • v.34 no.2
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    • pp.158-167
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    • 2016
  • This study evaluates potential sources of errors in k-NN(k-nearest neighbor) algorithm such as procedures, variables, and input data. Previous research has been thoroughly reviewed for understanding fundamentals of k-NN algorithm that has been widely used for short-term traffic states prediction. The framework of this algorithm commonly includes historical data smoothing, pattern database, similarity measure, k-value, and prediction horizon. The outcomes of this study suggests that: i) historical data smoothing is recommended to reduce random noise of measured traffic data; ii) the historical database should contain traffic state information on both normal and event conditions; and iii) trial and error method can improve the prediction accuracy by better searching for the optimum input time series and k-value. The study results also demonstrates that predicted error increases with the duration of prediction horizon and rapidly changing traffic states.

Study of Characteristics for Red Adhesive in Traditional Gold Thread (전통 편금사에 사용된 붉은 접착제 특성 연구)

  • Kim, Ji Eun;Yu, Ji A;Han, Ye Bin;Chung, Yong Jae
    • Journal of Conservation Science
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    • v.32 no.1
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    • pp.43-49
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    • 2016
  • Gold threads mean thread made by metal, it is composed various material to gold leaf, adhesive and media. The making technique and using materials of Gold threads are different from region, it can be used to distinguish country of origin. So this study finds characteristics and making techniques for flat gold threads of relics. Standard samples are made of animal glue, lacquer, red lacquer and etc. This materials are based on the scientific investigation of cultural gold threads properties. Then traditional techniques were estimated by comparison and analysis of actual cultural properties and standard samples. As a result of investigating cultural properties, it is similar to the standard sample which are mixed with red soil additives. After analysis of SEM-EDS, it is detected to Al, Si, Fe. So it is to the conclusion that traditional gold thread is made of adhesive mixed with red soil to improve gold color better. And this ways are similar with china that uses animal glue and red soil to adhesive and different from japan that uses lacquer to adhesive. Through this study, it will be used to data and reference for reappearance and making techniques of traditional gold threads.

Effect of Silk in Silk/PLGA Hybrid Films on Attachment and Proliferation of Human Aortic Endothelial Cells (실크/PLGA 하이브리드 필름에서 실크가 인간 대동맥 내피세포의 부착과 증식에 미치는 효과)

  • Lee, Jihye;Lee, Sojin;Kim, Seulji;Kim, Kyounghee;Kim, Younglae;Song, Jeongeun;Lee, Dongwon;Khang, Gilson
    • Polymer(Korea)
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    • v.37 no.2
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    • pp.127-134
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    • 2013
  • The vascular endothelial cells are the inner layers of blood vessels. It regulates the function of blood vessels and proliferation of vascular smooth muscle cells. Poly(lactide-co-glycolic acid) (PLGA) is a biodegradable synthetic polymer with a well-controlled degradation rate and an acceptable mechanical strength. It can be easily fabricated into many shapes. Silk consists of 18 amino acids. It found important for attaching cells cultured in vitro, and maintaining cell functions. In this study, we fabricated silk/PLGA biomaterial hybrid films of 0, 10, 20, 40 and 80 wt% silk. We performed MTT, SEM, ELISA, and immunocytochemistry analyses. We confirmed the adhesion and the proliferation of HAECs on silk/PLGA according to the content of silk, and 40 wt% silk/PLGA hybrid films have superior adhesion and proliferation properties. These results demonstrate that silk/PLGA hybrid films provide suitable surfaces for HAECs, and there is the effect of silk on cell growth and proliferation.

Comparative seed morphology of the tribe Alsineae (Caryophyllaceae) in Korea and its taxonomic implications (한국산 Alsineae (석죽과) 종자의 형태비교 및 분류학적 유용성)

  • Song, Jun-Ho;Kim, Ju-Mi;Ok, Min-Kyoung;Hong, Suk-Pyo
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.369-379
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    • 2015
  • The seed morphology of 25 taxa (8 genera) of Alsineae (Alsinoideae, Caryophyllaceae) in Korea was examined using stereo (SM) and scanning electron microscopy (SEM) in order to evaluate their diagnostic values and taxonomic implications. Circular, comma-shaped, ellipsoid, ovate, pyriform, reniform, and sub-circular seed shapes were identified. The seeds of the examined taxa range from 0.28 to 4.10 mm in length and from 0.33 to 3.10 mm in width; the smallest one was found in Sagina japonica, while the largest was in Honkenya peploides var. major. A hilar appendage was found in Cerastium, Honkenya, Moehringia, and strophioles were only observed in the genus Moehringia. Based on the examination of epidermal cells of the seed surface, four micromorphological types can be distinguished, viz., the colliculate-papillate (Col-Pap), colliculate-columellate (Col-Cll), colliculate-simple (Col-Sim), and smooth (Sm) types. Descriptions of the testa shape, margin, and the degree of elevation of the anticlinal cell walls, and the outer periclinal cell walls are summarized for the tribe. Taxonomic implications of the seed micromorphological characters were also discussed, in comparison with an earlier taxonomic system.

Effects of the Ge Prearmophization Ion Implantation on Titanium Salicide Junctions (게르마늄 Prearmophization 이온주입을 이용한 티타늄 salicide 접합부 특성 개선)

  • Kim, Sam-Dong;Lee, Seong-Dae;Lee, Jin-Gu;Hwang, In-Seok;Park, Dae-Gyu
    • Korean Journal of Materials Research
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    • v.10 no.12
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    • pp.812-818
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    • 2000
  • We studied the effects of Ge preamorphization (PAM) on 0.25$\mu\textrm{m}$ Ti-salicide junctions using comparative study with As PAM. For each PAM schemes, ion implantations are performed at a dose of 2E14 ion/$\textrm{cm}^2$ and at 20keV energy using $^{75}$ /As+and GeF4 ion sources. Ge PAM showed better sheet resistance and within- wafer uniformity than those of As PAM at 0.257m line width of n +/p-well junctions. This attributes to enhanced C54-silicidation reaction and strong (040) preferred orientation of the C54-silicide due to minimized As presence at n+ junctions. At p+ junctions, comparable performance was obtained in Rs reduction at fine lines from both As and Ge PAM schemes. Junction leakage current (JLC) revels are below ~1E-14 A/$\mu\textrm{m}^{2}$ at area patterns for all process conditions, whereas no degradation in JLC is shown under Ge PAM condition even at edge- intensive patterns. Smooth $TiSi_2$ interface is observed by cross- section TEM (X- TEM), which supports minimized silicide agglomeration due to Ge PAM and low level of JLC. Both junction break- down voltage (JBV) and contact resistances are satisfactory at all process conditions.

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A study on the nonadrenergic noncholinergic neurotransmitters in porcine gastric fundus (돼지 위저부 평활근의 비아드레날린 비콜린성 신경전달물질에 관한 연구)

  • Kim, Tae-wan;Na, Jun-ho;Lee, Jang-hern;Yang, Il-suk
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.119-128
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    • 1997
  • The relaxation of gastric fundus smooth muscles is the primary physiological event which induces the receptive relaxation of monogastric animals. L-arginine/Nitric oxide(L-arg/NO) system is known to mediate the inhibitory non-adrenergic non-cholinergic(NANC) neurotransmission in various tissues including gastrointestinal smooth muscles. The longitudinal smooth muscles of porcine gastric fundus showed fast relaxation during electrical field stimulation(EFS) and rebound contraction after EFS in NANC condition. So, the purpose of present study was elucidation of the neurotrasmitters related to the NANC relaxation and explanation of the relation between NANC relaxation and L-arg/NO system. The longitdinal smooth muscles of porcine gastric fundus were hung in the organ bath and under the presence of guanethidine($5{\times}10^{-5}M$), precontraction was induced by carbachol($1{\times}10^{-6}M$). The muscle responses to EFS and drugs were isomerically recorded. The rusults were summarized as follows. 1. The longtudinal muscles of porcine gastric fundus showed frequency-dependent relaxation and rebound contraction to electrical field stimulaton(1ms, 8V, 1~16Hz, 20sec, EFS). These responses were blocked by tetrodotoxin($1{\times}10^{-6}M$). 2. The relaxation and rebound contraction of the longitudinal muscles of porcine gastric fundus to EFS were inhibited by L-NAME($2{\times}10^{-5}M$). The inhibitory effect of L-NAME was antagonized by L-arginine($1{\times}10^{-3}M$), but not by D-arginine($1{\times}10^{-3}M$). 3. Exogenous NO($NaNO_2$, $1{\times}10^{-5}{\sim}1{\times}10^{-4}M$, pH=2.0) caused concentration-dependent relaxation as EFS did. 4. Methylene Blue($2{\times}10^{-5}M$), a soluble guanylate cyclase inhibitor, inhibited the relaxation and rebound contraction of the longitudinal muscles of porcine gastric fundus induced by EFS, but N-ethlmaleimide, a adenylate cyclase inhibitor, did not. 5. 8-Br-cGMP($1{\times}10^{-6}{\sim}3{\times}10^{-6}M$), permeable cGMP analogue, induced dose-dependent relaxation. but 8-Br-cAMP($1{\times}10^{-6}{\sim}3{\times}10^{-6}M$), permeable cAMP analogue, did not. Both did not evoked rebound contraction. 6. ${\alpha}$-chymotrypsin did not affect the relaxation of the longitudinal muscles of porcine gastric fundus. 7. Reactive blue 2($1{\times}10^{-4}M$, 40min) siginificantly inhibited the rebound contraction induced by EFS and inhibited contraction caused by exogenous ATP($1{\times}10^{-4}{\sim}1{\times}10^{-3}M$). These results suggests that NANC relaxation of the longitudinal muscles of porcine gastric fundus mainly mediated by NO and the rebound contraction is related to NO and other neurotransmitters.

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Effects of Angiotensin II on Isolated Cardiac Muscle and Aortic Strips in Rabbit (안지오텐신 II의 적출심근 및 대동맥 평활근에 대한 작용기전)

  • Kim, Kyu-Chan;Kim, Ki-Whan;Earm, Yung-E
    • The Korean Journal of Physiology
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    • v.17 no.1
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    • pp.45-54
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    • 1983
  • Contractile responses of myocardium and vascular smooth muscle to angiotensin II were studied in isolated rabbit papillary muscles and aortic helical strips, with respect to the sensitivity and the mechanism of action. All experiments were performed in $HCO-_3\;-buffered Tyrode solution which was aerated with $3%\;CO_2-97%\;O_2$ and kept pH 7.35 at $35^{\circ}C$. Action potentials were measured by conventional microelectrode technique in the papillary muscles. Helical strips of vascular smooth muscle were prepared from the descending thoracic aorta of the rabbit. Angiotensin II elicited a positive inotropic effect in doses from $10^{-8}$ to $10^{-6}\;M$, and this effect was dose-dependent and characterized by a symmetrical increase of maximum dP/dt during contraction and relaxation phase. Slow responses (or slow action potentials) were induced by A. II $(10^{-6}\;M)$ in the papillary muscle hypopolarized by 27 mM $K^+$. These A. II-induced slow action potentials were eliminated by verapamil (2 mg/l), but not affected by propranolol $(10^{-5}\;M)$. In aortic helical strips, contractile force was increased dose-dependently in the range of $10^{-10}{\sim}10^{-7}\;M$ A. II. $ED_{50}$ in aorta was $3{\times}10^{-9}\;M$ A. II, whereas that in paillary muscle was $2.5{\times}10^{-7}\;M$ A. II. A. II contracted vascular smooth muscle in depolarizing concentration of $K^+$ (100 mM $K^+$), and also produced a sustained contraction even in the presence of verapamil and regitine. The results of this experiment suggest that the primarily important physiological role of A. II is the action on the blood vessel, and the positive inotropic effect of A. II in papillary muscle results from the increase of slow inward $Ca^{++}$ current, and that A. II-induced contraction of aorta is independent of transmembrane potential and associated with promoting bet transmembrane $Ca^{++}\;-influx$ and the mobilization of cellular $Ca^{++}$.

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