• Journal of Periodontal and Implant Science
• /
• v.30 no.3
• /
• pp.491-504
• /
• 2000

In clinical therapy, the current goal of dental implants is to enhance quantity and quality of osseointegration. Surface roughness and oxide structure are considered to influence the behavior of adherent cells. The purpose of this study is to evaluate the effect of different surface treatment on cellular response. The attachment and proliferation of osteoblast-like cell on sandblasted, sandblasted and etched, thermal oxidated surfaces have been compared. Sandblasting was done with $Al_2O_3$ particles(grain size of $50{\mu}m$), etching was processed with $NH_4OH$ : $H_2O_2$ : $H_2O(1:1:5)$ at $90^{\circ}C$ for 1 minute. Thermal oxidation was followed sandblasting and etching at $400^{\circ}C$, $600^{\circ}C$, $800^{\circ}C$ for 2 hours. Measurement of surface roughness after the different treatment did not show any differences of Ra value between terated surfaces. Cell attachment and proliferation were increased during experiment period, but no difference was observed. SEM evaluation revealed a similar pattern of osteoblast-like cells, well attached with dendritic extension and producing numerous matrix vesicles on cell surface. The results of this study showed that oxide layer alteration by thermal oxidation did not affect the attachment and proliferation of osteoblast-like cells. This suggests the possibility that the cellular responses are further influenced by surface roughness than titaniun oxide structure.

• Journal of Periodontal and Implant Science
• /
• v.33 no.4
• /
• pp.739-745
• /
• 2003

Interleukine-1(IL-1)은 여러 가지 기능을 가진 싸이토카인으로써 미생물에 대한 면역반응을 일으킨다. IL-1의 유전자 다형성과 치주질환과의 관련성에 대한 많은 연구가 있어왔지만, 대부분이 백인을 대상으로 한 연구였다. 이후 중국인과 일본인을 대상으로 한 연구에서 IL-1의 유전자 다형성의 분포가 인종간에 차이를 보인다는 점이 발견되었다. 이번 연구에서는 치주적으로 건강한 한국인에서 IL-1${\beta}$-511, IL-1${\beta}$+3954, IL-1RN에 대한 유전자형의 분포를 조사하고자 하였다. 서울대학교 치과병원에 근무하는 치과의사, 치과위생사, 간호조무사 및 서울대학교 치과대학 4학년 학생 중 치주낭 깊이와 부착소실이 4mm 이하인 치주적으로 건강한 한국인 65명을 대상으로 하였다. IL-1${\beta}$-511, IL-1${\beta}$+3954, IL-1RN의 유전자 다형성은 분리한 DNA에 각 대립유전자에 특이성을 지닌 primer를 넣고 PCR(Polymerase Chain Reaction)법을 이용하여 증폭시킨후 전기영동법을 이용하여 각 대립유전자의 존재를 확인함으로써 결정하였다. IL-1${\beta}$-511 대립유전자 11, 대립유전자 12, 대립유전자 22의 유전자형에 대하여 각각 23.1%, 49.2%, 26.2%의 분포를 보였다. IL-1${\beta}$+3954의 유전자 다형성은 대립유전자 11, 대립유전자 12의 유전자형에 대하여 각각 89.2%, 10.8%의 분포를 보였으며, 대립유전자 22의 유전자형을 갖는 사람은 한명도 발견되지 않았다. IL-1RN의 유전자형은 5가지의 대립유전자 중에서 1, 대립유전자 2, 대립유전자 4만일 발견되었으며, 대립유전자 11, 대립유전자 12, 대립유전자 14의 유전자형이 86.2%, 12.3%, 1.5%로 분포하였다. 이를 바탕으로 각 대립유전자의 발생빈도 계산한 결과 IL-1${\beta}$-511에서는 대립유전자 1과 2의 비율이 거의 유사하였으나 (47.7%, 52.3%), IL-1${\beta}$+3954, IL-1RN에서는 대립유전자 1이 90%이상 발견되었으며, 또한 대립유전자 1외의 다른 대립유전자가 발견된 경우, 모두 이형접합체였다. 이 연구는 IL-1${\beta}$-511, IL-1${\beta}$+3954, IL-1RN에 대한 유전자형의 분포를 조사한 것으로 한국인에서 이들 유전자의 유전자형의 분포는 백인에서의 분포와 차이를 보이고 있었다. 이후 치주질환자의 유전자형 분포와의 비교로 치주질환과 IL-1${\beta}$-511, IL-1${\beta}$+3954, IL-1RN의 유전자다형성과의 관련성에 관한 추가적인 연구가 필요할 것으로 여겨진다.

• Journal of Periodontal and Implant Science
• /
• v.23 no.1
• /
• pp.109-126
• /
• 1993

Refractory periodontitis manifest progressive attachment loss in a rapid and unrelenting manner regardless of the type or frequency of therapy applied. The purpose of this study was ta evaluate the relation between the level of cytokines in GCF and periodontopathic microflora with disease activity of refractory periodontitis. Selection of patients with refractory periodontitis (7 males, 3 females) were made by long term clinical observation including conventional clinical history and parameters. Teeth that showed pocket depth greater than 6mm were selected as sample teeth. Subjects were examined at baseline and after 3 months. Prior to baseline test, individual acrylic stent was fabricated. Reference grooves were made on each sample tooth site. Pocket depth and attachment loss were measured by Florida Probe. Gingival index was measured at 4 sites each sample teeth. Disease activity was defined as attachment loss of ${\ge}$ 2.1mm, as determined by sequential probing and tolerance method. The pattern and amount of alveolar bone resorption was observed with quantitative digital subtraction image processing radiography. Morphological analysis of subgingival bacteria was taken by phase contrast microscopy. Predominant cultivable bacterial distribution and frequency were compared between disease-active and disease-inactive site using immunofluorescence microscopy and selective microbial culturing. Levels of $interleukin-l{\beta}$, 2, 4, 6 and $TNF-{\alpha}$ in GCF and blood serum sample were quantified by ELISA. In active sites, P. intermedia was significantly increased to compare with inactive site. $IL-1{\beta}$, IL-2, IL-6 and $TNF-{\alpha}$ in GCF were increased in active sites and IL-2 in serum was increased in active patients significantly. Alveolar bone loss in active site was correlated with $IL-1{\beta}$, IL-2 in GCF. And loss of attachment in active site was correlated with IL-2 in GCF. These results demonstrate that IL-2 in serum, $IL-1{\beta}$, IL-2, IL-6 and $TNF-{\alpha}$ in GCF, P, intermedia might be used as possible predictors of disease activity in refractory periodontitis before it is clinically expressed as attachment loss and quantitative alveolar bone change.

• Journal of Periodontal and Implant Science
• /
• v.26 no.3
• /
• pp.771-778
• /
• 1996

The assessment of alveolar bone changes on dental radiographs to indicate progression of periodontal diseases or healing response to therapy is routine procedure. However, the diagnostic accuracy in detecting small alveolar bone changes is very limited. Recently, guided bone regeneration therapy is popular, but the quantification of new bone is somewhat difficult with conventional evaluation method. To quantificate the amount of new bone, various evaluating methods have been introduced including histomorphometry, radiomorphometry, biochemical analysis, X-ray probe microanalysis, scanning electron microscope backscatter method. In this study, guided bone regeneration using resorbable membrane with & without PDGF-BB is quatificated through histomorphmetry to evaluate the efficacy of histomorphometric analysis. 4 beagle dogs and 8 Sprague-Dawley rats were selected as experimental animals. In beagle dog experiment, $4{\times}4mm$ Class II defects were created in maxillary both second premolars, and biodegradable membrane containing PDGF-BB(experimental group) were covered over one defect, and same membrane without PDGF-BB(control group) were covered over the other defect. At 2 weeks, 5 weeks after surgery, each beagle dogs were sacrificed, and the tissues were treated by undecalcified fixation. In Sprague-Dawley rat experiment, 5mm round defect were created in temporal bone, the same membranes were covered on the defects. At 1 week, 2 weeks after surgery, each rats were sacrificed, and undecalcified fixation were taken. After grinding tissue specimen, we analyse them histomorphometrically using image analysis system. In beagle dog 2 weeks specimens, new bone formation area were $0.03123mm^2$ in experimental group,and $0.03012mm^2$ in control group. At 5 weeks specimens, $0.15324mm^2$ in experimental group, and $0.09123mm^2$ in control group. In Sprague-Dawley rat specimens, new bone fomation area were $0.20448mm^2$ in 1 week experimental group, $0.03604mm^2$ in 1 week control group. At 2 weeks specimens, $0.46349mm^2$ in experimental group, $0.17741mm^2$ in control group. The results indicated that histomorphometric analysis of new bone formation using image analysis system is very effective quantification method to evaluate the efficacy of treatment modalities.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.36 no.1
• /
• pp.91-95
• /
• 2009

The use of stainless steel crowns are indicated for restoration of primary or permanent molars with proximal dental caries, extensive dental caries, or previous pulp treatment with increased danger of tooth fracture. Stainless steel crowns were introduced by Humphrey in 1950. For their improved durability, longevity, and success rate, they have been strongly considered for restoring extensive and multi-surfaced dental caries of molars in pediatric dentistry. However, they also have shortcomings, such as possibility of pulpal exposure or damaging proximal surface of adjacent teeth. In addition, when oversized stainless steel crowns are used, eruption of the adjacent permanent teeth may be disturbed by their prominent margin. As a means to compensate the shortcomings of stainless steel crowns, use of orthodontics bands may be considered. It is an alternative restoration method, where an orthodontic band is placed on a tooth first and cavity is restored with filling material, such as composite resin, glass ionomer, or amalgam. The use of an orthodontic band is indicated for molar restoration with cervical dental caries, extensive dental caries, enamel hypoplasia, or previous pulp treatment. Because it requires shorter chair time compared to stainless steel crown, its application is very useful for children with poor behavior. However, restoration using an orthodontic band requires good oral hygiene after its application. This case report illustrates the conservative restoration of primary molars and permanent molars with extensive dental caries using orthodontic bands.

• Journal of the Korean School Mathematics Society
• /
• v.6 no.1
• /
• pp.65-85
• /
• 2003

The purpose of this research is to increase mathematical problem solving abilities VIa STAD evaluation after completing classes. to which ST AD group study is applied, and promoting the learning accomplishments of students by developing gradual self-leading learning materials about the research project on ' How to use an hour math class efficiently\ulcorner ' For this purpose, the items below were studied. Firstly, gradual self-leading learning materials were developed and applied which were composed of textbook abstracts, basic problems, developing problems and intensive problems rather than existing textbooks. Secondly, the ST AD group study model was selected and applied which invokes competitions among small groups of which learning goals were clear. individual responsibility was important. and successive opportunities were equal. The evaluation using STAD at each end of a chapter was announced instantly using the EXCEL scoring system. Though the results of experimental classes were limited in their size. experimental time, and class selection, there were meaningful changes in the aspect of being able to heighten the accomplishment desire of students by inducing voluntary competitions among small groups without any student omitted. As the result of applying this research to my class, the ST AD group study using gradual self-leading learning materials invoked the interests of students and increased learning accomplishments via increasing problem solving abilities in mathematics. The ST AD group study was easy to use by beginning teachers, and its process was simple. It increased interactions among students and learning motives because its compensation system was open to all students. Among various studying methods for small groups. STAD group study is expected to be widely used for mathematics classes.

• The Journal of Engineering Geology
• /
• v.22 no.1
• /
• pp.83-94
• /
• 2012

Slopes are commonly formed both above and below roads located in mountainous terrain and along riversides. The Buk-sil site, a cut slope formed below the road, collapsed in October, 2010. A field investigation determined the causes of failure as improper drainage of valley water from the slope above the road and direct seepage of road-surface water. These factors may have accelerated the collapse via complex interaction between water and sub-surface structures such as bedding. Projection analysis of the site showed the possible involvement of plane, wedge, and toppling failure. Safety factors calculated by Limit Equilibrium Analysis for plane and wedge failure were below the standard for wet conditions. The wetness index, analyzed using topographic factors of the study area, was 9.0-10.5, which is high compared with the values calculated for nearby areas. This finding indicates a high concentration of water flow. We consider that water-flow control on the upper road is crucial for enhancing slope stability at the Buk-sil site.

• Journal of Environmental Impact Assessment
• /
• v.26 no.5
• /
• pp.331-343
• /
• 2017

The important changes in water environment management in Korea can be summarized as the enactment of Act on the Integrated Control of Pollutant-Discharging Facilities. Therefore water quality impact assessment should be reexamined and be revised. This study examines the present water quality impact assessment items (permissible discharge limits, standards for effluent water quality including Total Pollutant Load Management System) and considers the land use regulation for water quality conservation and NVZs(Nitrate Vulnerable Zones of EU and England). It also considers lately adopted standards(maximum discharge standards, permissible discharge standards, and marginal discharge standards etc) based on Act on the Integrated Control of Pollutant-Discharging Facilities and then compares Korean BAT and its counterpart control technology of U.S.A. And it also compares the items of water quality impact assessment with those of Integrated Control of Pollutant-Discharging Facilities, based on EIS reporting items. This study suggests five improvement measures for water quality impact assessment. First reflection of discharge impact analysis on impact prediction and assessment, second reflection of permissible discharge standards on agreed standards in the EIA procedure, third, reflection of diversified BAT on mitigation measures in the EIA procedure, forth introduction of land use regulation such as NVZs, finally strengthening linkage between water quality items and land use items etc.

• Horticultural Science & Technology
• /
• v.33 no.2
• /
• pp.233-241
• /
• 2015

Kimchi cabbage 'Choon Gwang' cultivar that was grown in highlands in Gangneung was subjected to predrying, room cooling, and forced air cooling, and then packed with/without 0.02 mm HDPE film to investigate the effect of postharvest treatment on quality characteristics during 8 weeks storage at $2^{\circ}C$ (RH $90{\pm}5%$). Weight loss in forced air cooling and room cooling was lower than 3-4% with 0.02 mm HDPE film liner treatment during storage. However, it was only below 10% in room cooling without liner treatment and forced air cooling without liner treatment led to the highest weight loss, above 15%. Conversely, the control had lower weight loss than the others. SSC was $2-4^{\circ}brix$ for all treatments and there was no difference between postharvest treatments and liner treatments. Color index and firmness both showed no differences with/without 0.02 mm HDPE film and postharvest treatments. In sensory evaluation, forced air cooling with liner treatment was effective, with the highest score, especially in appearance and crispness. After 6 weeks, control kimchi cabbage without liner treatment was damaged seriously in appearance and the internal color had changed to brown. Room cooling and predrying with liner treatment changed the start of internal browning to after 8 weeks storage.

• Journal of Internet Computing and Services
• /
• v.21 no.3
• /
• pp.29-39
• /
• 2020

Online communities, such as Internet portal sites and social media, have become popular since they allow users to share opinions and to obtain information anytime, anywhere. Accordingly, an increasing number of opinions are manipulated to the advantage of particular groups or individuals, and these opinions include falsified product reviews and political propaganda. Existing detection systems are built upon the characteristics of manipulated opinions for one particular time period. However, manipulation tactics change over time to evade detection systems and to more efficiently spread information, so detection systems should also evolve according to the changes. We therefore propose a system that helps observe and trace changes in manipulation tactics. This system classifies opinions into clusters that represent different tactics, and changes in these clusters reveal evolving tactics. We evaluated the system with over a million opinions collected during three election campaigns and found various changes in (i) the times when manipulations frequently occur, (ii) the methods to manipulate recommendation counts, and (iii) the use of multiple user IDs. We suggest that the operators of online communities perform regular audits with the proposed system to identify evolutions and to adjust detection systems.