• Korean Journal of Plant Resources
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• v.18 no.2
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• pp.207-215
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• 2005

Korean ginseng(Panax ginseng C.A. Meyer) roots have long been known as the best medicinal plant and its pharmaceutical bio-activities have been proven by scientific analyses of their components - ginsenosides, acidic polysaccharides, phenolic compounds, fatty acids and organic acids etc. Ginseng hairy roots and callus have been cultured in vitro for stable supply of ginseng material. In this study, the amount of ginsenosides, fatty acids, acidic polysaccharides, phenolic compounds and organic acids in ginseng hairy roots and callus were compared. Higher amount of ginsenoside was found in ginseng hairy roots than ginseng callus. Higher amount of saturated fatty acid (palmitic acid) was found in callus and higher amount of unsaturated fatty acid (linoleic acid) was found in hairy roots. Acidic polysaccharide and phenolic compounds were contained by the same amount in both hairy roots and callus. Organic acids were found more in hairy roots.

• Applied Biological Chemistry
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• v.47 no.2
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• pp.216-221
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• 2004

Changes in physiological functionality of giant embryonic rice by germination process were investigated using 70% ethanolic extract of the rices. Physiological functionality was evaluated by determining the reducing power, phenolic compound content, GABA content, and antimutagenicity. The results showed that the order of reducing power of the non-germinated rices was Nampung giant embryonic rice>normal rice> Whachung giant embryonic rice, however, the activity was high as the order of Whachung giant embryonic rice> Nampung giant embryonic rice> normal rice by germination process. About 3-fold activity increase was observed for Whachung giant embryonic rice, while, the activity of Nampung giant embryonic rice and normal rice decreased by the same treatment. The phenolic compound content of three rice cultivars were found to be almost same levels. Germination of rice increased the content of phenolic compounds by 2.6-fo1d without any considerable changes for both Nampung giant embryonic rice and normal rice. The GABA contents was highest in Whachung giant embryonic rice, followed by Nampung giant embryonic rice, normal rice in either germination or non-germination condition. The germination increased the GABA contents by more than 2.4-fold for all rice cultivars tested. We also found an increase in the antimutagenic activity by germination process for all cultivars, where the activity was the greatest for Whachung cultivar.

• Analytical Science and Technology
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• v.13 no.5
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• pp.683-692
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• 2000

The volatile organic compounds emitted from food wrapping films when it was used at high temperatures. However, the testing method based on solvent extraction may not be suitable for determination of volatile organic compounds from food wrapping films. Therefore, thermal extraction device was investigated in the study for reflection of actual usage conditions at home and restaurant. In this study, PVC food wrapping films were exposed to normal use condition controlled from $50^{\circ}C$ to $120^{\circ}C$ and collected on sorbent trap. Then, emitted VOCs from the films were analyzed with On-column cryofocusing-GC/MS system. The major VOCs emitted from films were $C_{12}-C_{18}$ paraffinic hydrocarbons, dimethylphthalate, alkylphenols, known as endocrine disrupting chemical, such as tetramethylbutylphenol, tert-butylphenol, and nonylphenol. The concentration of tetramethylbutylphenol emitted from PVC films was $0.07ng/cm^2$ at $50^{\circ}C$, rapidly increased with temperature, $3.94ng/cm^2$ at $100^{\circ}C$.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.546-551
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• 2002

Nutritional composition and phenolic compounds of raw propolis collected from falseacacia (Robinia pseudoacacia L.) and chestnut tree (Castanea crenata), and their 70% ethanol extracts of propolis (EEP) were analyzed. Propolis had high crude lipid content, but no significant differences in general compositions in terms of collection area and plant origins. Mineral contents varied greatly depending on the plant origins, with falseacacia propolis showing the highest mineral content. Sixteen amino acids were analyzed, among which aspartic acid content was the highest at $328.4{\sim}410.6\;mg%$ and methionine the lowest at $0{\sim}21.1\;mg%$. Extraction yield for EEP was relatively high at $64.2{\sim}81.9%$, and total polyphenol and flavonoid contents were $13.9{\sim}23.7$ and $8.6{\sim}10.8%$, respectively. HPTLC and HPLC analysis on the phenolic compounds revealed the overall chromatographic patterns were almost equal, showing similar polyphenol compositions between the propolis. About 16 peaks were identified by HPLC analysis, among which 6 peaks of p-hydroxy benzoic acid, caffeic acid, ferulic acid, benzoic acid, cinnamic acid, and chrysin were identified.

• Culinary science and hospitality research
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• v.21 no.5
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• pp.171-179
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• 2015

This study was conducted to investigate the antioxidant activity and the bioactive compounds found in 70% ethanol extracts taken from Sanguisorbae officinalis L. leaves(SO) cultivated in Korea. The extracts were tested for their total phenolic contents (TPC), total flavonoid contents (TFC), phenolic compounds, and antioxidative activities using various in vitro assay such as DPPH, ABTS radical scavenging activity, FRAP activity, and reducing power. The TPC and TFC were found to be $119.3{\pm}1.54mg$ gallic acid and $59.6{\pm}1.43mg$ rutin at mg of 70% ethanol extracts, respectively. Catechin was the major material among the phenolic compounds in SO extracts. The DPPH radical scavenging activity, ABTS radical scavenging activity, FRAP activity, and reducing power of SO extracts were increased in a dose-dependent manner. These results suggest that SO extracts could be considered as a good source of natural antioxidants and functional food ingredient.

• Journal of the Korean Chemical Society
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• v.33 no.4
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• pp.406-412
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• 1989

Indirect photometric detection of aliphatic compounds such as alcohols, ketones and esters was investigated in high-performance liquid chromatography, These samples possessing nonchromophore or weak chromophore could be indirectly detected by a UV detector with addition of UV absorbing, p-chlorophenol or p-nitrophenol in mobile phase as detection reagent, and submicrogram detection limits were achieved. Some mixtures of samples were also able to be separated and quantitated with good resolution and comparatively high sensitivity under optimum conditions.

• Korean Journal of Food Science and Technology
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• v.31 no.1
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• pp.147-152
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• 1999

HPLC was used for determining polyphenols which are known as the functional compounds in the unripe apples Fuji and Aori. The changes in their contents caused by heat treatments were monitored. The carbohydrate contents in Fuji and Aori were 14.1% and 13.5% respectively. Both apple juices showed pH 3.2 and $8.0\;brix^{\circ}$, which were relatively low levels. The major polyphenols were composed of (+)-catechin, chlorogenic acid, (-)-epicatechin, and tannic acid. Total polyphenol contents in Fuji and Aori were 0.11, 0.12% by Folin-Denis method. HPLC analysis of polyphenols showed that four major components were contained by 0.06% in Fuji and 0.07% in Aori. Chlorogenic acid was three times higher in Fuji than in Aori. After water blanching, the area percent of polyphenols resulted in an increase by 3.54% in Fuji but a decrease by 2.93% in Aori. Pasteurization of juices led to decrease by 1.39% and 3.31% respectively. Blanching and pasteurization of unripe apple juices induced negligible changes in polyphenol contents during storage. During concentration, polyphenol contents increased in proportion to the concentration of unripe apple juices.

• The Korean Journal of Food And Nutrition
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• v.22 no.4
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• pp.548-553
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• 2009

Qualitative properties- polyphenol compounds(total phenolics, anthocyanin, flavonoids, tannins), color parameters and antioxidant activities- of wild grape wine having different aging periods were studied. The contents of total phenolics and anthocyanin decreased significantly in accordance with aging terms(p<0.05). The total phenolics and anthocyanin of the oldest wine(WG3) represented only 43% and 45% of it's related compound in the wine aged for one year(WG1), respectively. The contents and the degree of polymerization of tannins, including condensed tannins, also decreased but not showed statistically significant. However, The contents of flavonoids of the 2 year old wine(WG2) decreased significantly related to that of WG1, remaining, then, almost constant at that levels during aging. The L* values, b(yellow) values and the hue (hab) values were increased in long aged wines(WG2, WG3), while the intensity of color(CI) and, the a(red) parameter value decreased. The antiradical activity of wild grape wines was shown to be high(87 EDA%) in WG1. The corresponding coefficient of tannin concentrations with antiradical activity($r^2$=0.9189) suggests that the tannin content may be strongly related to the antioxidant properties of wild grape wines.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2011.10a
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• pp.19-19
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• 2011

예비실험 중 한국의 울릉도에 자생하고 취나물로 이용되고 있는 섬쑥부쟁이(Aster glehni Franchet et Sckmidt, Compositae)가 페놀성 화합물 함량이 높고 peroxynitrite 소거효과가 높았기 때문에 이를 이용한 추출물의 제조를 통한 건강기능식품을 창출하고자 하였다. 건강식품을 위한 추출물 제조를 위하여 물과 에탄올만을 이용하여 페놀성 화합물 고함유 추출물의 제조가 가능하고 높은 peroxynitrite 소거효과를 나타내는 추출물을 결정하고자 하였다. 즉, 추출용매로 Water-EtOH 비율을 조절하여 추출하여 추출물의 함량, HPLC를 이용한 caffeoylquinic acid(CQ) 화합물의 함량분석 및 peroxynitrite 소거효과를 비교하였다. Water-EtOH (7:3)으로 추출한 섬쑥부쟁이 추출물은 CQ 함량이 높고, 높은 peroxynitrite 소거효과, 항경련효과, 진정효과 및 항비만효과 등이 나타났다. 건강기능식품을 위한 추출물 제조시 한국에서는 물과 에탄올만이 허용되기 때문에 CQ 함량이 높은 추출물의 제조가 가능하고 높은 peroxynitrite 소거효과를 유지할 수 있는가에 관한 실험 이외에 마우스에서 선택된 추출물의 진정효과 및 항경련효과 실험을 수행하였다. 진정효과와 항경련효과를 위하여 마우스에서 각각 pentobarbital로 유도한 수면연장효과와 PTZ로 유도한 경련에 대한 효과를 측정하여 유의성있는 활성이 확인되었으므로 이 추출물을 이용한 건강기능식품으로의 이용이 가능하다고 생각된다.

• Journal of the Korean Wood Science and Technology
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• v.35 no.6
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• pp.145-151
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• 2007

To investigate the chemical constituents of Acer tegmentosum, the bark were collected, air-dried and extracted with 70% aqueous acetone. Then it was successively partitioned with n-hexane, $CH_2Cl_2$, EtOAc and $H_2O$. Repeated Sephadex LH-20 column chromatography on the EtOAc soluble fraction gave five phenolic compounds. Their structures were elucidated as (+)-catechin (1), (-)-epi-catechin (2), Q-epicatechin-3-O-gallate (3), gallic acid (4) and 6'-0-galloylsalidroside (5) on the basis of spectroscopic evidences using $^1H-NMR$, $^{13}C-NMR$, 2D-NMR and MS spectroscopy, (-)-epicatechin-3-Ogallate (3), gallic acid (4), 6'-Ogalloylsalidroside (5) have not been reported in this plant yet.