• Title/Summary/Keyword: 페놀물질

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Some Physiological Activity of Phenolic Substances in Plant Foods (식물성 식품중 페놀성 물질의 몇가지 생리활성)

  • Lee, Jung-Hi;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.26 no.3
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    • pp.317-323
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    • 1994
  • Nine plant foods (persimmon leaf, perilla seed, Chinese quince, ginger root, walnut, mugwort leaf, arrowroot, buckwheat and sorghum) rich in phenolic substances were examined for their effects on the digestive enzymes, food-poisoning bacteria and mutagenicity/antimutagenicity by Ames test. Among tested samples, Chinese quince significantly inhibited the $\alpha-amylase$ activity (97%), exhibiting an uncompetitive inhibition type. Protease activity was inhibited by Chinese quince (86%), persimmon leaf (51%) and mugwort leaf (20%), in which mugwort extract exhibited a noncompetitive type. Lipase was activated >50% by all samples. The inhibition of $\alpha-amylase$ was highly correlated with the content of condensed tannin (r=0.89) and the inhibition of protease, with total phenolic content (r=0.84). Total phenolies fraction of tested samples showed the growth inhibition toward E. coli. Streptococcus faecalis and Salmonella enteritidis, in which the effect of perilla, sorghum and arrowroot was the highest for E. coli. Standard phenolics and food samples did not show any mutagenicity toward Salmonella typhimurium TA98 and TA100. Tannic acid inhibited the mutation of the two strains by benzo[a]pyrene whereas total phenolics fractions of Chinese quince and walnut exhibited antimutagenicity to a lesser extent.

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The Antioxidative and Antibrowning Effects of Citrus Peel Extracts on Fresh-cut Apples (Citrus 과피 추출물의 항산화 및 사과 슬라이스에 대한 항갈변 효과)

  • Park, Miji;Kim, Gun-Hee
    • Korean Journal of Food Science and Technology
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    • v.45 no.5
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    • pp.598-604
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    • 2013
  • This study was designed to evaluate the usability of the following citrus peel extracts (CPEs): Citrus sinensis (orange), C. unshiu (mandarin orange), C. limon (lemon), and C. paradise (grapefruit) as natural antibrowning agents. Overall, 0.1% of the CPEs were effective in reducing the browning of apple slices. The appearance of apple slices dipped in C. limon peel extracts (LPE) were found to be excellent and their Hunter L and ${\Delta}E$ values were similar to the values obtained when apple slices were dipped in ascorbic acid. The pH values of the different CPEs were not significantly different from each other, but were higher than that of ascorbic acid. LPE was found to possess the highest total phenolic content, flavonoid content, DPPH radical scavenging activity, and copper ($Cu^{2+}$) chelating activity. All these results suggest that citrus peel extracts, especially lemon peel extract, can be used as natural antibrowning agents.

Antioxidative Activity of Solvent Extracts from Synurus excelsus and Synurus palmatopinnatifidus (큰수리취 및 국화수리취 용매추출물의 항산화 활성)

  • Lee, Kwang-Jae;Yun, In-Ju;Kim, Hee-Yeon;Kim, Kyung-Hee;Kim, Young-Jin;Kim, Dong-Woon;Lim, Sang-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.12
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    • pp.1893-1897
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    • 2010
  • The total polyphenol contents and antioxidative activities of water and ethanol extracts from Synurus excelsus and Synurus palmatopinnatifidus var. palmatopinnatifidus were determined. Total polyphenol contents of ESE and ESP were 195.7 and 216.2 mg/g, and were higher than WSE (74.7 mg/g) and WSP (77.4 mg/g). The total flavonoid contents were also higher in ESE (176.6 mg/g) and ESP (148.8 mg/g) than WSE and WSP. DPPH radical scavenging activities of ESE and ESP (73.1 and 73.4%) were higher than WSE and WSP as like total polyphenol and flavonoid contents. Although the DPPH radical scavenging activities of the extracts were proportional to total polyphenol contents, there are no differences between water extracts and ethanol extracts of Synurus excelsus and Synurus palmatopinnatifidus on ABTS radical scavenging activities. Moreover, all the solvent extracts (ESE, ESP, WSE and WSP) had no SOD-like activity. These results suggest that consideration for adoption of method is necessary to evaluate antioxidative activity of extracts from plants including vegetables.

Antioxidant Activity and Functional Components of Corn Silk(Zea mays L.) (옥수수 수염의 항산화 활성과 기능성분 분석)

  • Ku, Kang-Mo;Kim, Soon-Kwon;Kang, Young-Hwa
    • Korean Journal of Plant Resources
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    • v.22 no.4
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    • pp.323-329
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    • 2009
  • In order to find out superior corn line which has a strong antioxidant activity in the corn silk, antioxidant compounds and antioxidant activity were measured from various inbred and F1 lines. As a result, the contents of phenolic and flavonoid compounds range from 532 mg/100 g to 3,274 mg/100 g and from 980 mg/100 g to 2,420 mg/100 g respectively. Absorbance at 517 nm for contents of anthocyanins ranges from 0.05 to 0.76. Correlation coefficients between antioxidant compounds and various antioxidant assays such as DPPH, ABTS and FRAP were analyzed. Correlation coefficients between antioxidant activity and phenolic compounds and anthocyanin were significantly high in ABTS and FRAP assays. Considering acidic extraction condition and intervention between anthocyanins and DPPH solution, ABTS assay and FRAP assay are more suitable methods to evaluate antioxidant activity of corn silks. Especially, ABTS assay is thought to be the best method among three assays because the antioxidant activity in ABTS assay showed high correlation with phenol, flavonoid and anthocyanin compounds respectively. Among the samples, S15 which showed the highest contents of total phenolic compounds and the most potent antioxidant activity in ABTS and FRAP assay will be a good source for functional material.

Change in Polyphenol Content, Antioxidant Activity, and Antioxidant Enzyme Status of Cowpea During Germination (동부 발아기간 중 폴리페놀 함량, 항산화성 및 항산화효소 활성 변이)

  • Chon, Sang-Uk
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.60-67
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    • 2013
  • A laboratory experiment was conducted to determine the content of phenolics and flavonoids, antioxidant activity and antioxidant enzyme activity for the extracts from cowpea seed and sprouts. Plant length and weight of cowpea sprouts were significantly increased until 7 days after seeding. Total phenolics level [mg chlorogenic acid equivalents (CAE) $kg^{-1}$ DW] was highest in dry seed (DS) extracts of cowpea ($63.9mg\;kg^{-1}$), followed by imbibed seed (IS) ($56.8mg\;kg^{-1}$) and 1-day-old sprout (1DOS) extracts ($46.4mg\;kg^{-1}$), and significantly reduced with increase of sprout age (p < 0.05). The antioxidant activity of the methanol extracts from all the samples showed same tendency to the results of total phenolics level, and dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in DS (87.3%) and IS (41.2%) than in cowpea sprouts from 1DOS to 7DOS, ranging from 17.1 to 30.4%. Antioxidant enzymes, APX, POX, and POX activities were highest in 7DOS and lowest in DS. SOD activity showed much higher activity in sprouts and in seeds. Correlation coefficient between physiological-active substance and the activity was highest between APX and CAT activities ($r^2$= 0.9574). Especially, total phenolics content was more highly correlated with antioxidant or with antioxidant enzyme activities than was total flavonoid level.

Effect of Wood vinegar on Tomato Seedling Growth and Nutrient Uptake (토마토 유묘생육 및 양분흡수에 관한 목초액의 영향)

  • 김승환;최두희;이상민;남재작;김한명;손석용;송범헌
    • Korean Journal of Organic Agriculture
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    • v.11 no.2
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    • pp.103-113
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    • 2003
  • The chemical properties of oak tree wood vinegar and the effect of wood vinegar on the tomato seedling were investigated to apply wood vinegar efficiently to the organic - and natural farming system. On the basis of the results from chemical properties of the oak tree wood vinegar, mineral nutrient contents of wood vinegar was low. Therefore, wood vinegar could not be a suitable nutrient source for the plant growth at 500∼1000 times dilution level. which commonly used in the farming, if only wood vinegar is supplied for the nutrient source for the plant growth. The application of wood vinegar increased root growth up the 500 times dilution level while decreased shoot growth. Furthermore. the anion concentrations such as nitrate and phosphate of the plant were decreased by the application of wood vinegar while cation concentrations such as K. Ca. and Mg were increased. Phenolic compounds of wood vinegar such as chlorogenic acid and ferulic acid enhanced the root growth. Interestingly the application of ferulic acid increased both root and shoot growth at the level of 10$^{-4}$ M concentration. It indicated that the effect of wood vinegar on the production of healthy plant seedling may be due to the beneficial root growth by phenolic compounds such as chlorogenic acid and/or ferulic acid of the wood vinegar. However. the effect of the wood vinegar on the plant growth could be influenced by synergism or antagonism of different phenolic compounds in wood vinegar used. In addition. drench in the soil of wood vinegar may be more beneficial compared to foliar application for the improvement of root activity and plant growth.

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Effect of Microwave on the Antioxidant Activity of Barley Bran (맥강의 항산화능에 대한 Microwave의 영향)

  • Bae, Sung-Moon;Kim, Jeong-Han;Cho, Cheol-Woo;Jeong, Tae-Joon;Kim, Jeong-Mok;Lee, Seung-Cheol
    • Applied Biological Chemistry
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    • v.44 no.4
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    • pp.235-239
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    • 2001
  • Effect of microwave treatment to barley bran was tested on antioxidative ability. Treatments of microwave at 100 W and 200 W, changed color of barley bran to brown, but carbonation was happening at 440 W. The electron donating abilities of barley bran was not reduced when treated with 100 W and 200 W microwave treatment for 10 minute. According to TBARS, the barley bran extract treated with 100 W and 200 W intensity of microwave for 10 min maintained the inhibition of lipid peroxidation, while the extract from 440 W microwave treatment for 5 min did not. Total phenolic contents were reduced with increased intensity of microwave treatment. The antioxidant activity of barley bran was not affected by microwave treatment at 100 W and 200 W, while the 440w microwave treatment reduced the activity due to carbonation of barley bran and decreased phenolic compounds.

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Removal Characteristics of Phenol at Advanced Oxidation Process with Ozone/Activated Carbon Impregnated Metals (오존/촉매 산화공정에서 금속담지 활성탄을 이용한 페놀의 분해 특성)

  • Choi, Jae Won;Yoon, Ji Young;Park, Jin Do;Lee, Hak Sung
    • Applied Chemistry for Engineering
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    • v.23 no.3
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    • pp.302-307
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    • 2012
  • Advanced oxidation processes (AOP) such as O3/activated carbon process and O3/catalysts process were used to compare the decomposition of phenol. Catalysts such as Pd/activated carbon (Pd/AC), Mn/activated carbon (Mn/AC), Co/activated carbon (Co/AC) and Fe/activated carbon (Fe/AC) were prepared by impregnation of Pd, Mn, Co and Fe into the activated carbon of pellet form, respectively. Based on an hour of reactions, the following descending order for the decomposition ratios of dissolved O3 to the 1.48 mg/L of saturated dissolved O3 was observed: Mn/AC (45%) > Pd/AC (42%) > Co/AC (33%) > AC (31%) > Fe/AC (27%). The removal efficiencies of phenol were also arranged in the descending order of AOP as follows: Mn/AC (89%) > Pd/AC (85%) > Co/AC (77%) > AC (76%) > Fe/AC (71%). The remaining ratios (C/Co) of TOC (total organic carbon) after an hour of experiments were arranged in the ascending order of AOP as follows : Pd/AC (0.29) < Mn/AC (0.36) < AC (0.40) < Co/AC (0.49) < Fe/AC (0.51). However, the catalytic effects in the Co/AC and the Fe/AC processes were little in comparison with O3/AC process. The maximum concentrations of intermediates such as hydroquinone and catechol formed from the decomposition of phenol were arranged in the ascending order of AOP as follows: Pd/AC < Fe/AC < Co/AC < AC < Mn/AC. In the case of Pd/AC process, these intermediates were almost disappeared after an one hour of reaction.

Effect of functional material addition on the growth and physiological activity materials in Pleurotus ostreatus (기능성물질 첨가시 느타리버섯 생육 및 생리활성물질의 변화)

  • Park, Jae-Seong;Choi, Jae-Sun;Choi, Seong-Yeol;Song, In-Gyu;Yun, Tae;Lee, Jun-Soo;Koo, Chang-Duck
    • Journal of Mushroom
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    • v.7 no.3
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    • pp.91-97
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    • 2009
  • This expriment was carried out on culture media which affect growth, development and physiological effect to Pleurotus ostreatus, when aqueous ferrum, red pepper powder, leaves of ginkgo biloba were added to culture media. The yield of fresh mushroom per 850ml PP bottle in chunchu-neutari was highest by 144g in 34.9g aqueous ferrum added, ferrous content don't affected to yield of mushroom, but variety of Pleurotus ostreatus affect to yield. Mineral content of fruit body and culture media in Suhan-neutari was different to mineral kind, ferrous content of furit body was similar to 0.31~0.43mg/kg when ferrous content of media was increased in 0.89mg/kg to 10.8mg/kg. The more content of red pepper powder in culture media was high, the more capsaicin content in fruit body was high. transferred capsaicin was highest by 6 mg/100g in Suhan-neutari. Total phenolics content were higher in culture media which was added aqueous ferrum and red pepper powder, but correlation don't showed total phenolics content among antioxydant activity, reducing power, chelating capacity. Macrophage activation was higher in Suhan-neutari than other Pleurotus spp.

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Comparison of Antioxidant Compounds and Activities of Different Parts of 'Shine Muscat' Grape with Extraction Condition (포도 '샤인머스켓'의 부위별 추출조건에 따른 생리활성 물질 함량 및 항산화 활성)

  • Mi Hee Choi;Hyun Jin Choi;Eun Young Nam;In Myung Choi
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.85-85
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    • 2020
  • 껍질째 먹는 청포도 품종인 '샤인머스켓'은 망고포도라고 불릴 정도로 단맛이 강하고 풍부한 머스켓향을 가지는 것이 특징이다. 또한 포도알이 크고 무핵처리에 의해 씨가 제거되어 소비자 만족도가 높아 최근 각광받고 있는 포도 품종이다. 샤인머스켓에 대한 국내연구는 주로 재배조건에 따른 과실특성 위주로 보고되고 있어, 본 연구는 소비가 늘고 있는 샤인머스켓의 기능성에 대한 기초자료를 제시하고자 부위별 추출조건에 따른 총폴리페놀, 총플라보노이드 함량 및 항산화 활성을 조사하였다. 샤인머스켓은 2019년 11월에 경북 상주지역에서 생산된 것으로, 과피와 과육의 2가지로 나누어 동결건조하였으며, 추출용매(증류수, 에탄올, 메탄올)와 추출용매농도(30%, 50%, 70%, 100%) 조건에 따라 시료 무게대비 10배의 용매를 가한 후 초음파방법으로 2시간 추출하였으며, 이를 2회 반복하였다. 부위별 총폴리페놀과 총플라보노이드 함량은 과육보다 과피에서 유의적으로 높게 나타났으며, 항산화 활성 또한 과피에서 월등히 높은 값을 나타냈다. 추출조건에 따른 생리활성 물질의 함량과 항산화 활성은 과육과 과피에서 다르게 나타났는데, 과육의 경우 메탄올 추출 시 활성이 높게 나타난 반면, 과피는 에탄올 추출 조건에서 활성이 더 높게 나타났다. 과육의 경우, 70% 메탄올 추출 조건에서 가장 높은 활성을 보였으며, 총폴리페놀 0.99 mg CE/g dw, 총플라보노이드 0.31 mg CE/g dw, DPPH 라디칼 소거능 2.94 mM TE/g dw, ABTS 라디칼 소거능 6.82mM TE/g dw, ORAC 11.40 mM TE/g dw, FRAP 4.92 mM Fe(II)/g dw이었다. 이와 달리 과피는 30% 에탄올 추출 조건에서의 활성이 유의적으로 높게 나타났으며, 총폴리페놀 10.23 mg CE/g dw, 총 플라보노이드 5.22 mg CE/g dw, DPPH 라디칼 소거능 44.72 mM TE/g dw, ABTS 라디칼 소거능 123.25 mM TE/g dw, ORAC 208.38 mM TE/g dw, FRAP 96.24 mM Fe(II)/g dw이었다. 이상의 결과를 볼 때, 껍질째 샤인머스켓을 섭취하는 것은 기능성 측면에서 유익하며, 추후 부위를 나누지 않은 샤인머스켓을 대상으로 추출조건 설정 및 기능성 평가에 대한 연구를 진행할 예정이다.

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