• Clinical and Experimental Reproductive Medicine
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• v.26 no.2
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• pp.231-238
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• 1999

The objective of this study was to examine the effect of developmental stage and embryo age of in vitro produced bovine blastocysts after vitrification and thawing. In vitro cultured day 8 blastocysts after IVF were equilibrated 20% ethylene glycol (EG) for 3 min. and were vitrified using EFS40, which is consisted of 40% EG, 18% ficoll, 0.3M sucrose and 10% FBS added in mDPBS for 30 sec. before being plunged into $LN_2$. Also, survival in vitro was assessed by re-expansion and hatching or hatched at 24 hand 48 h postwarming, respectively. The results obtained in these experiments were summarized as follows; 1) When the embryos were cultured for 8 day after IVF, 41.0% of the cleaved embryos developed to the blastocysts (early; 7.6%, expanded; 22.9%, hatching; 4.6% and hatched; 5.9%). 2) When the embryos were exposed or vitrified to the freezing solution, the re-expansion of vitrified embryos (73.3%) was significantly lower than that of control and exposed embryos (100, 97.0%) (p<0.05). But the formation rate of hatching or hatched blastocysts of vitrified embryos (66.7, 46.7%) at 48h after thawing was similar to that of exposed embryos (66.7, 39.4%) but not control (100, 100%) (p<0.01). However, in the total cell numbers of those developed hatched blastocysts, there were not significantly different among the treatment groups. 3) When the embryo survival rates by different developmental stage were examined, the re-expansion was not different among the groups $(64.5{\sim}75.6%)$. After warming 48 h, the hatching and hatched formation of early blastocysts (25.8, 9.7%) was significantly lower than those of expanded (69.7, 39.4%) and hatching blastocysts (53.3, 43.3%) (p<0.05). 4) In addition, when the expanded blastocysts at day 7, 8 and 9 were vitrified, the re-expansion of day 8 and 9 embryos was significantly lower than that of day 7 (day 7; 93.9%, day 8; 75.8% and day 9; 87.5%) (p<0.05). However, the rates of development to hatched blastocysts were no difference among the groups (day 7; 36.4%, day 8; 36.4% and day 9; 31.3%). These results suggested that in vitro produced expanded or hatching blastocysts can be efficiently cryopreserved by the two-step vitrification method using EFS40.

• Proceedings of the SAREK Conference
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• 2008.06a
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• pp.583-590
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• 2008

최근 작업자 숙소 및 이송용 외에 작업용으로 사용 가능한 Barge선의 일종인 Accommodation Work Barge 선의 건조량이 증가 추세인 바 현재 중국에서 건조 중인 해당 선박의 HVAC & Refrigeration system에 대하여 정리하였다. 본 시스템은 R404A Direct expansion 냉각방식 (직접팽창방식)이 적용되었으며 HVAC system 중 Air conditioning 부분에 대해서는 선박의 각 Deck 기준으로 Zoning 하여 개별적인 Air handling unit와 Condensing unit를 구성하였으며 (각 unit의 용량은 필요용량의 100%), 냉동 창고의 Refrigeration system은 해당 격실 (육고, 어고, 야채고)에 각각 Unit cooler를 설치하고, Condensing unit를 기계실에 설치하였다. 장비는 전체 용량 100%에 대하여 항시 운전하는 100% 용량의 장비와 비상시에 운전하는 100% 용량의 예비 장비로 구성된다. 냉동 창고에 인접한 Dry provision store는 냉동 창고와는 별개로 중앙 공조기로부터의 냉각 공기를 이용하여 Spot cooling하였다. 본 System의 구성에 대한 장점 및 단점은 아래와 같다. 1. Air conditioning system이 각 Zone에 대하여 구성되므로 각 Zone에 대하여 제어가 가능하다. 2. Air con. 실에 Air handling unit와 Condensing unit가 설치되므로 냉매 배관의 길이가 짧다. 3. Air con. 실에 Air handling unit와 Condensing unit가 설치되므로 실내의 Maintenance space 상에 여유가 없다.

• 한국지구과학회:학술대회논문집
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• 2010.04a
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• pp.104-105
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• 2010

울산 퇴적 분지에 분포하는 신현층의 이암층 노두에서 이매패에 속하는 Anadara 를 비롯한 여러 종류의 연체동물 화석을 채집하였다. Anadara 화석은 해성 기원의 연체동물로 Vicarya japonica Yabe and Hatai와 함께 소위 Vicarya-Anadara 군집에 속한다. 이 군집은 우리나라의 제3기 생층서를 이해하는데 가장 중요한 정보를 제공한다. 여기에서는 Andara 화석의 형태적 특징과 내부 구조를 고찰하고, 현생종과 비교하였다. 신현층에서 산출된 Anadara 화석은 1속 2종으로 구성된다. 각의 형태적 특징은 각의 외형이 삼각형에 가깝고, 표면에 25~30개의 방사륵이 발달해 있으며 인대면은 삼각형 모형으로, 교치선은 직선이다. 각의 내부 구조를 박편상에서 관찰한 결과, 4개의 층으로 구성되어 있다. Anadara 화석과 현생종 Scapharca subcrenata 을 비교한 결과, 각의 외형은 조금 다르나 내부구조는 서로 거의 유사하다. A. (H.) kakehataensis의 내부 구조는 최외층, 외층, 광휘층, 내층으로 구성되어 있다. 외층은 교차판 구조(crossed- lamellar structure)로 되어 있다. 또한 성장선이 발달되어 있으며, 복연부에 불규칙한 균열이 부분적으로 나타난다. 이는 외부로 노출되어 건조되고 다시 물기를 머금은 상태가 반복되어 수축 팽창에 의한 것이라고 생각되며, 별개의 층은 아니다. 내층은 엽상구조(foliated structure)가 관찰된다. 외층과 내층 사이에 아라고나이트로 구성된 광휘층이 나타나며, 능주 구조(prismatic structure)를 보이는 얇은 층이다. 최외층이 나타난다. A. (H.) daitokudoensis의 내부 구조는 A. (H.) kakehataensis와 서로 거의 유사하고, 내층, 외층, 광휘층 및 최외층의 4개의 층으로 구성된다. 엽상 구조인 내층, 교차판 구조인 외층, 능주 구조인 광휘층 및 최외층은 유기질층으로 되어있다.

• Proceedings of the KSME Conference
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• 2001.11b
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• pp.426-431
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• 2001

This paper experimentally investigates the characteristics of dual coaxial jet issuing from inner supersonic nozzle and four kinds of outer converging nozzle of 40, 50, $60^{\circ}$ and $70^{\circ}$ in outer ejection angle. The pressure ratio of the stagnation to the exit ambient pressures in the inner supersonic nozzle of constant expansion rate is 7.5, which is corresponded to the condition of a slightly underexpanded, and that of outer nozzle is 4.0. Flow visualizations by using of shadowgraph method, impact pressure and centerline static pressure measurements are presented. It is found that the jet structure is changed significantly by the variation of outer nozzle ejection angle. Impact pressure level is lower and undulation of static pressure is higher, as the injection angle of outer jet increases.

• Korean Journal of Food Science and Technology
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• v.28 no.2
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• pp.285-291
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• 1996

The effect of various packaging methods on Kimchi quality was investigated in order to develop the packaging techniques for preventing commercial Kimchi Products from ination and explosion due to fermentative gas evolved during storage and distribution. Kimchi was packaged in different methods; atmospheric (AP), check-valved (CV), double (DP) and vacuum (VP) packaging. The quality of Kimchi during storage at $10^{\circ}C$ was evaluated ill terms of gas composition, free volume, pH, titratable acidity, color index, lactic acid bacteria and sensory properties. $O_2$ concentration decreased to about 1% at $6{\sim}8$ days of optimal ripening time in all packages except DP, in which it remained around 20%. $CO_2$ concentration reached $70{\sim}90%$ after $6{\sim}8$ days in AP, CV and DP, while it stayed around 10% in DP. The free volume in AP had typical sigmoidal curve similar to $CO_2$ concentration changes. It remained almost constant in CV and DP, but started to increase at the late stage of storage In VP. There was no significant influence of packaging methods on the other quality attributes such as pH, titratable acidity, color index (L.b/a), lactic acid bacteria count and sensory evaluation score. As results, it could be proposed to employ CV and DP method as the effective packaging techniques for preventing commercial Kimchi products from inflation.

• The Journal of Engineering Geology
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• v.30 no.2
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• pp.185-198
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• 2020

High-density rapid expansion material is a method that increases the solid volume of injection materials due to hydration and foam reactions at the same time as spraying. It is an effective method for securing ground stability, restoring subsidence, and loading during construction of structures. In this regard, through the mechanical experiments of injection materials, the stability of the foundation ground of the structure and the effect of increasing the endurance using site construction were analyzed. The results of the experiment showed that the unit weight of soil decreased by 10.5% after injection of the filling material, and the allowable support for the structure was deemed safe, and the subsidence by each section after ground improvement was determined to be safe at 2.28, 1.55 and 0.46 cm, respectively, with an acceptable subsidence of less than 5 cm. After the field test, five inclinometers were installed on the top floor of the target building to measure the displacement of the X and Y axes. As a result of the measurement, no displacement related to the phenomenon of inequality or subsidence cracks of the structure was measured for about 16 months (509 days) after construction. This can be judged to be a sufficient increase in the stability of the ground after the injection of rapid expansion.

• Development and Reproduction
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• v.2 no.2
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• pp.179-187
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• 1998

The effects of prostaglandins in hatching and implantation have been studied but the results were various, and those are not well known by the embryonic stage. The present study examined the effects of prostaglandin $E_2$(PG $E_2$) and prostaglandin $F_2$$_{\alpha}$ (PG $F_2$$_{\alpha}$) on the expansion and hatching of mouse embryos by embryonic stage. Also we tried to measure the concentration of prostaglandins of morula, expanded, and hatching embryos. In early morula stage embryos, high concentration of PG $E_2$(>100$\mu$M) showed cytotoxicity but PG $F_2$$_{\alpha}$ did not. The hatching was inhibited all groups but not gave negative effects on expansion. In 84 hr and 96 hr stage embryos, the hatching rate was decreased at all treatment groups but not inhibited the expansion. When combine prostaglandin with indomethacin, the hatching rate was increased significantly compared to the prostaglandin-treated groups, and as lower and lower the PG $E_2$ concentration, the hatching rate increased to the control level. The embryonic synthesis of PG $E_2$ increased dramatically but that of PG $F_2$$_{\alpha}$ increased gradually. PG $E_2$ showed cytotoxicity at early stage embryos much than late stage embryos, but PG $F_2$$_{\alpha}$ did not. Hatching was inhibited by the high PG $F_2$$_{\alpha}$ concentration. It is suggested that the inhibition of hatching might be at resulted from cytotoxicity of PG $E_2$ on embryo. However, it is thought that the mechanisms of inhibition of hatching are different between PG $E_2$ and PG $F_2$$_{\alpha}$. In conclusion, it can be suggested that PG $E_2$ and PG $F_2$$_{\alpha}$ concerned with the expansion and hatching, and their effects on hatching were different by the embryonic stage.$/ concerned with the expansion and hatching, and their effects on hatching were different by the embryonic stage.

• Journal of Astronomy and Space Sciences
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• v.23 no.3
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• pp.217-226
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• 2006

We report three sawtooth oscillation events observed at geosynchronous orbit where we find quasi-periodic (every 2-3 hours) sudden flux increases followed by slow flux decreases at the energy levels of ${\sim}50-400keV$. For these three sawtooth events, we have examined variations of the outer boundary of the outer radiation belt. In order to determine L values of the outer boundary, we have used data of relativistic electron flux observed by the SAMPEX satellite. We find that the outer boundary of the outer radiation belt oscillates periodically being consistent with sawtooth oscillation phases. Specifically, the outer boundary of the outer radiation belt expands (namely, the boundary L value increases) following the sawtooth particle flux enhancement of each tooth, and then contracts (namely, the boundary L value decreases) while the sawtooth flux decreases gradually until the next flux enhancement. On the other hand, it is repeatedly seen that the asymmetry of the magnetic field intensity between dayside and nightside decreases (increases) due to the dipolarization (the stretching) on the nightside as the sawtooth flux increases (decreases). This implies that the periodic magnetic field variations during the sawtooth oscillations are likely responsible for the expansion-contraction oscillations of the outer boundary of the outer radiation belt.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.9
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• pp.626-633
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• 2020

This study examined the behavior of the ground by comparing the methods using the results of the Terzaghi formula and the ground investigation data and method considering the dilatancy effect for a circular tunnel using the finite element method. In the case of the Terzaghi formula, the tunnel load can be overestimated and cause overdesign. The method using the results of the ground investigation data cannot be applied when a reasonable coefficient of earth pressure is not determined. This is because it behaves completely differently from the actual behavior, and unexpected problems can occur. In the case of the method considering the dilatancy effect, however, both the strength enhancement effect can be considered through the dilatancy angle and relative density. Therefore, the tunnel load was calculated most reasonably using the method considering dilatancy. Finite element analysis using the geotechnical survey results showed that the tensile stress acts at the top of the tunnel when the upper soil of the tunnel is shallow. On the other hand, additional verification is necessary, such as a comparison with the field measurement results. Through additional research, if normalized, the tunnel load can be calculated reasonably at the time of tunnel design, and safe and economical design is possible.

• Journal of the Korean Geotechnical Society
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• v.19 no.2
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• pp.227-236
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• 2003

The evaluation of the reliable strength and deformation characteristics of weathered granite masses is very important for the design of geotechnical structure under working stress conditions. Various types of laboratory test such as triaxial compression test can be performed to determine the strength parameters. However, it is very difficult to obtain the representative undisturbed samples on the site and also the rock specimen cannot represent rock mass including discontinuities, fracture zone, etc. This study aims to investigate the strength and deformation characteristics of granite masses corresponding to its weathering and develop a practical strength parameter evaluation method using the results of PMT. To predict weathering intensity and strength parameters of the weathered granite masess in the field, various laboratory tests and in-situ tests including field triaxial test and PMT are carried out. Based on the results of weathering index tests, the classification method is proposed to identify the weathering degree in three groups for the weathered granite masses. Using the analytical method based on the Mohr-Coulomb failure criteria and the cavity expansion theory, the strength parameters of rock masses were evaluated from the results of PMT. It shows that weathering intensity increases with decreasing the strength parameters exponentially. The strength parameters evaluated with the results of PM almost coincide with the results of field triaxial test.