• KSBB Journal
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• v.11 no.6
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• pp.663-668
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• 1996

In the production of recombinant protein using E. coli, phage lysogen system can be usefully applied for simultaneously achieving protein production at high cell concentration and recovery by cell disruption in the same bioreactor. A major drawback of this system is that the intracellular product and complex broth components are mixed together in culture broth and hence purification efficiency is reduced. With the E. coli double-lysogen system, the expanded bed adsorption is very useful because the pretreatment processes in a routine bioseparation process can be done in a single column operation, and therefore may contribute towards lowering the operating cost of overall recovery/purification process. In the operation of EBA, it has been observed that the change in broth feed volume does not influence much the protein recovery in a tested range. The amount of protein adsorption per mL of resin was increased from $3.44{\times}106unit to 5.28{\times}106unit$ by doubling the column length. By two-fold increase of the column diameter, the ratio of protein concentration in eluent to that in feed was increased from 0.8 to 2.1. It is concluded from the present investigation that the increase of column length and diameter is necessary to enhance the protein adsorption amount per volume of resin and protein concentration in the eluent. The development of resins with various physical properties will be necessary for more extensive application of EBA.

• Journal of Navigation and Port Research
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• v.27 no.1
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• pp.97-102
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• 2003

Wire ropes are used in a myriad of various industrial applications such as elevator, mine hoist, construction machinery, lift, and suspension bridge. Especially, the wire rope of crane is important component to container transfer. If it happens wire rope failures during the operation, it may lead to safety accident, economic loss by productivity decline and so on. To solve this problem, we developed remote wire rope fault detecting system, and this system is consisted of 3 parts that portable fault detecting part, signal processing part and remote monitoring part. All detected signal has external noise or disturbance according to circumstances. So, we applied to discrete wavelet transform to extract a signal from noisy data. It is verified that the detecting system by de-noising has good efficiency for inspecting faults of wire ropes in service. As a result, by developing this system, container terminal could reduce expense because of extension fo wire ropes exchange period and could competitive power. Also, this system is possible to apply in several field such as elevator, lift and so on.

• Journal of the Korea institute for structural maintenance and inspection
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• v.15 no.2
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• pp.61-69
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• 2011

Fiber Reinforced Plastic (FRP) sheets have been widely used to retrofit and rehabilitate RC structures, while in case of retrofitting steel structures, there are no codes and researches. It stems from configuration of member and characteristics of bonding behavior. This study focused on the static behavior of steel beams reinforcement by AFRP sheets. The main objective of the experimental programme was the evaluation of the force transfer mechanism, the increment of the beam load carrying capacity and the bending stiffness. A bending test was conducted on a H-shaped steel beam, with aramid FRP sheets bonded to its flanges. The mid-span deflection and the strain from three points along AFRP sheets were recorded Test results exhibit that the increment of the load-carrying capacity with reference to a mid-span deflection level of 15 mm(1/125mm of the clear span) was equal to 9.4% and for the two layers case, an elastic stiffness increment is slightly higher than one layer case.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.9 s.339
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• pp.85-96
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• 2005

A ' Virtual Private Network (YPN) over Internet' has the benefits of being cost-effective and flexible. However, given the increasing demands for high bandwidth Internet and for reliable services in a 'VPN over Intemet,' an IP/GMPLS over DWDM backbone network is regarded as a very favorable approach for the future 'Optical VPN (OVPN)' due to the benefits of transparency and high data rate. Nevertheless, OVPN still has survivability issues such that a temporary fault can lose a large amount of data in seconds, moreover unauthorized physical attack can also be made on purpose to eavesdrop the network through physical components. Also, logical attacks can manipulate or stop the operation of GMPLS control messages and menace the network survivability of OVPN. Thus, network survivability in OVPN (i.e. fault/attack tolerant recovery mechanism considering physical structure and optical components, and secured transmission of GMPLS control messages) is rising as a critical issue. In this Paper, we propose a new path establishment scheme under shared risk link group (SRLG) constraint for physical network survivability. And we also suggest a new logical survivability management mechanism by extending resource reservation protocol-traffic engineering extension (RSVP-TE+) and link management protocol (LMP). Finally, according to the results of our simulation, the proposed algorithms are revealed more effective in the view point of survivability.