• Journal of dental hygiene science
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• v.16 no.6
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• pp.409-414
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• 2016

The purpose of this study was to evaluate the antibacterial effect of tea tree oil in denture cleaners. A self-curing denture resin was used to make the experimental specimen ($12mm{\times}2mm$). A saline solution was used as the control. To observe surface changes after cleaning, the microhardness and color of the experimental specimen's surface were analyzed. For the antibacterial activity test, Candida albicans was used. The microhardness and color of the surface remained unchanged after cleaning. The result of the antibacterial activity test revealed that the tea tree oil-containing solutions had a more enhanced antibacterial effect than did the saline solution. Therefore, these results suggest that the tea tree oil-containing solution is a promising denture cleaners.

• Journal of Life Science
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• v.18 no.12
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• pp.1644-1650
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• 2008

The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-MS. ${\beta}$-Terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%) and limonene (4.65%) were the major components in the oil. The results tested by MTT assay indicated that the oil showed no cytotoxic effect, at concentrations up to 5%, for less than 3h. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction ($RC_{50}$) against ABTS radicals ($1.6{\pm}0.02%$) was slightly lower than DPPH radicals ($2.6{\pm}0.29%$). The direct contact and vapor-phase antibacterial activity of the oil were also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticus. All the Gram-negative bacterial strains tested showed more sensibility to the oil than the Gram-positive strains when compare to the effect of gentamycin. On the other hand, the vapor phase of the essential oil against S. aureus exhibited strongest inhibitory effect.

• Journal of Advanced Technology Convergence
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• v.2 no.1
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• pp.23-31
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• 2023

This study was described about the EEG response results of tea tree oil fragrance for the development of functional shampoo additives. In the case of experimenters (1) the intensity change of the right brain alpha wave in electroencephalographic (EEG) response was increased after inhalation of the tea tree oil fragrance. (2) the change of physical tension and stress both the left brain and the right brain in EEG response showed a tendency to decrease after inhalation compared to before inhalation of the tea tree fragrance. (3) the mental distraction and stress was decreased after experimenters inhales the tea tree oil fragrances. These means the tea tree fragrances have both mental stability and stress relief. From above results, we prepared the functional shampoo additives with tea tree oil, and the we determined the alpha wave in EEG response after the functional shampoo additive fragrance for experimenters. As results, (4) the alpha wave intensity in left and right brain was significantly decreased after inhaling the functional shampoo additives fragrance. (5) physical tension and stress changes were decreased after inhalation of functional shampoo additive fragrance. (6) mental distraction and stress changes were also decreased after inhalation of functional shampoo additive fragrance. , From above results, the functional shampoo additives could be used as physical and mental relievers. Furthermore, the functional shampoo additives have the effects of scalp calming when the experimenter was treated shampoo with the functional shampoo additives.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.3
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• pp.256-262
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• 2017

Essential oil from herb is known to exert pharmacological effects on the human body. In this study we investigated the antibacterial activity of 4 essential oils (teetree, rosemary, melisa, and lavender), as well as the blended mixture oil of teetree, rosemary, and melisa (TRM) on three bacteria, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Antibacterial analysis was performed using the standard disk diffusion method, and minimum inhibition concentration was determined by the broth microdilution method with different concentrations of essential oils (0.5, 1, 2 and 3 mg/mL). After incubation at $37^{\circ}C$ for 24 h, the antibacterial activity was assessed by measuring the zone of growth inhibition surrounding the disks. Herb oil with the inhibition zones showed varied values ranging from6 to 25 mm. However, the components of herb oil of TRM are as highly active as the teetree oil against pathogens, generating large inhibition zones for both gram negative and positive bacteria (13~22 mm and 8 mm inhibition zones). In the analysis for MIC, TRM showed growth-inhibitory effects at 0.0625% for S. aureus and E. coli, and 1.25% for P. aeruginosa. This result demonstrated that the anti-microbial activity of TRM was greater than a single herb oil, including oxacillin, rosemary, and teetrea. As a single herb oil, both rosemary and teetrea also had an anti-microbial effect by itself, and we can expect that the blended oil mixture may exert a synergistic effect against multidrug resistant bacteria, suggesting its future application in natural preservative agents for health food and cosmetics.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.431-437
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• 2018

Acinetobacter baumannii is categorized as a red alert pathogen that is increasingly associated with a high mortality rate in infected patients because of its resistance to extensive antibiotics. This study evaluated the antibacterial activities of some essential oils (tee tree, rosemary, and lavender oils) against 18 clinical isolates of multidrug-resistant A. baumannii (MRAB). The carbapenemase screening Hodge test showed that all 20 strains of A. baumannii were resistant to imipenem. The identification of multidrug-resistant microbes was carried out using the VITEK system. The antimicrobial activity of essential oils was tested by a disk diffusion method against MRAB. In the disk diffusion method, tea tree showed the largest increase in inhibition size compared to lavender oil, and rosemary had no antibacterial effect. These results proved the antimicrobial effect of multidrug resistance A. baumannii. Tee tree oil would be a useful alternative natural product for the treatment and prevention of most common human pathogens and MRAB infections. This is expected to be used as an antimicrobial agent, such as hand disinfectant using natural essential oil in the future.

• Journal of Korean society of Dental Hygiene
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• v.17 no.4
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• pp.613-620
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• 2017

Objectives: The purpose of this study is to investigate the antibacterial effect of Streptococcus mutans of tea tree ingredient. Methods: The experimental groups were each given with different concentrations (30 or 50 vol%) of tea tree prepared in saline solution. The control group applied only saline solution. The tea tree coating of the specimen were examined under a scanning electron microscope. For the antibacterial activity test of the tea tree, the contact angle of the tea tree- coated specimen's surface was analyzed. The antibacterial effect against Streptococcus mutans was determined by counting the colony forming units (CFU). The statical statics were evaluated by using one-way ANOVA and paired t-test. Results: The tea tree treated group of hydrophilic more than non treated group. Antibacterial experiments demonstrated that tee tree solution was effective against Streptococcus mutans. However there was no significant difference in depending solution concentration groups. Conclusions: The antimicrobial activity of the tea tree containing solution showed its potential for use as coating for denture and medical materials.

• Journal of Life Science
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• v.22 no.10
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• pp.1344-1351
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• 2012

We studied the physical, chemical, biological, and antimicrobial effects of eight types of essential oils used in the cosmetics industry: lavender, tea tree, rosemary, juniper berry, Chamaecyparis obtusa, cypress, cedar wood, and pine. Lavender oil had a linalyl acetate (an ester chemical compound) content of 48% and radical scavenging activity of 22.36% at 5,000 ppm. Tea tree oil had radical scavenging activity of 43.94% at 5,000 ppm and antimicrobial activity against S. aureus, S. epidermidis, S. mutans, and C. albicans in each 6, 3.5, 6.5, and 5 mm, respectively. Chamaecyparis obtusa oil had the highest acidity (pH 2.64) compared with the other oils, and sesquiterpene compounds were found to have 19.20%. Cedar wood oil had the highest specific gravity and refractive index compared to the other oils and had a sesquiterpene content of 99.73%. The radical scavenging activity of cedar wood essential oil exceeded 39.68% at 5,000 ppm. The clear zone, indicating antimicrobial activity against P. acnes, P. ovale, and C. albicans, was 3.5, 6, and 6 mm, respectively, at a concentration of 1% cedar wood oil. Results showed that with a high sesquiterpene content, the antioxidant effect was generally, but not always, high, suggesting that this is determined according to composition of the compound rather than presence of each antioxidant. The results indicate that antimicrobial activity is determined by the existence of each antimicrobial ingredient rather than terpene composition.

• Korean Journal of Adult Nursing
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• v.17 no.2
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• pp.276-286
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• 2005

Purpose: The study is to investigate the effects of tea tree oil gargling on oral cavity micro-organism growth and on the perceived discomfort of patients receiving chemotherapy. Methods: A nonequivalent control group non-synchronized design was used to determine the effects of tea tree oil gargling on oral cavity for 20 second after using it for one week, twice a day. The sample consisted of two groups of patients receiving chemotherapy : 19 patients in experimental and 20 patients in control group. The instruments used in the study were Oral Assessment Guide(OAG), a measure of perceived symptoms on oral cavity, and a test of oral mucosal micro-organism culture. The data were analyzed using chi-square test, repeated measure of ANOVA, and Pearson correlation coefficient. Results: There was no significant difference between the two groups in micro-organism culture test of oral mucosa. The experimental group showed a lower number and fewer kinds of micro-organisms than the control group. Conclusion: It is considered that use of tea tree oil is effective in infection control of the oral cavity.

• Journal of the Korean Society of Fashion and Beauty
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• v.3 no.3 s.3
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• pp.31-40
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• 2005

The reason that aromatherapy began to draw attention again as the alternative therapy is because the essential oils that are used for aromatherapy not only allow the human body to maintain homeostasis but also have an effect on curing a mental feeling of fatigue and because these efficacies and effects are proceeding with being proved scientifically. Also, with coming to be high in a voice of concern about severally side effects that the modern medicine and the synthetic chemistry have, the aromatherapy came to be paid attention as the replacement therapy that can substitute for and supplement this. In particular, at the time of requiring a study on a substance to substitute for this after having been announced in Japan for 1998 a risk of Zincpyrithione that is being used as a component of dandruff treatment, it is thought that the aroma oil that was known for having an anti-dandruff effect has a merit of being easy in a user's access, thus it will be effective in curing the symptom of dandruff. Accordingly, this study conducted the following experiment in a bid to examine whether the aroma oil has the anti-microbial effect on the actual malassezia. First of all, it measured the minimum concentration of aroma oil in which the growth of malassezia is inhibited, by carrying out MIC(Minimum Inhibition Concentration) test in terms of anti-microbial activity against Malassezia furfur KCTC 7744 while using oils of Patchouli, Rosemary, Lavender, and Tea tree among aroma oils that are being used for dandruff treatment. As a result of that, Tea tree oil has the most excellent anti-microbial activity, and the measurement result of anti-microbial activity was shown in order of Lavender oil>Rosemary oil>Patchouli oil. Next, it carried out the is actually eased by applying Tea tree oil directly to the scalp of dandruff. In consequence of the experiment, the massage of Tea tree oil indicated the result of relieving the symptoms of dandruff such as scale, itch, pain, inflammation, and sebum, and among these symptoms of dandruff, it was shown to be most effective in easing itch. Especially, this clinical experiment was indicated that the massage of Tea tree oil has the most effect on relieving itch of targets with a dry-skin type who had no experience of dandruff treatment.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.243-245
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• 2016

The insecticidal toxicities of seven essential oils against Ricania sp. nymph and adults, newly recorded pest, were evaluated using leaf-dipping and spray methods. At 72 h exposure time, the $LC_{50}$ values of the essential oils of Artemisia vulgaris, Anethum graveolens, Citrus paradisi, Melaleuca alternifolia, Melaleuca leucadendron, Neroli bigarade and Tagetes erecta were 251.02, 387.31, 286.21, 353.54, 275.64, 424.55, $165.68mg/cm^2$ when using the leaf dipping method against Ricania sp. nymphs, and 278.31, 402.11, 306.11, 379.64, 298.22, 459.56, $183.21mg/cm^2$ when using the spray method against Ricania sp. adults, respectively. These results indicate that the essential oil of the Tagetes erecta can be potentially used as new natural insecticidal agents against Ricania sp. nymph and adults.