• Korean Journal of Environment and Ecology
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• v.6 no.2
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• pp.201-217
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• 1993

The purpose of this is to suggest objective basic data for the use development and conservation management of the national park through the quantitative analysis of the visul quality included in the physical environment of the Sobaeksan National Park, for this. spatial images structure of physical elements have been analyzed by factor analysis algorithm and degree of visual quality have been measured mainly by questionnaries. Result of this thesis can be summarized as follows. Factors covering the spatial image of the Sobaeksan National Park landscape have been found to be the overall synthetic evaluation appeal, spatial, natural quality and dignity factors such as the overall the synthetic evaluation. spatial and appeal yield high factor scores. As for the factors determining the degree of visual quality of clear of valley, peculiarity of configuration natural of trail, harmony of suitable artificial planting and temple.

• Korean Journal of Environment and Ecology
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• v.9 no.2
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• pp.232-249
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• 1996

The purpose of this is to suggest objective basic data for the use development and conservation management of the national park through the quantitative analysis of the visual quality included in the physical environment of the Odaesan National Park, for this, spatial images structure of physical elements have been analyzed by factor analysis algorithm and degree of visual quality have been measured mainly by questionnaries. Result of this thesis can be summarized as follows. Factors covering the spatial image of the Odaesan National Park landscape have been found to be the pverall synthetic evaluation, physical, spatial, natural quality and, appeal factors such as the overall the synthetic evaluation, spatial and appeal yield high factor scores. As for the factors determining the degree of visual quality of clear of valley, peculiarity of configuration, natural of trail, harmony of suitable artificaial planting and temple.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.433-441
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• 1995

Mammary tissue-specificity of the caprine $\beta$-lactoglobulin promoter appears to be secured by repression in non-expressing cells. In order to identify the mechanism of the negative regulation, the upstream promoter sequence of the caprine $\beta$-lactoglobulin gene was analyzed in detail. The repression was mediated by the upstream flanking sequence from -47O to -205. The sequence could repress the promoter activity of $\beta$-lactoglobulin in either orientation. The effect of the putative negative regulation element of caprine $\beta$-lactoglobulin on heterlogous promoters, however, varied: the promoter activity of herpes simplex virus thimidine kinase was either repressed or activated by the sequence depending on its orientation, while the SV4O early promoter was activated rather than repressed. The regulatory sequence involving the putative negative regulatory element was strongly shifted with the nuclear extract from non-mammary HeLa and CV-1 cells, while only weak shift was observed with that of mammary HC11 cells. Such correlation between repression and factor binding suggests that the protected regions in foot-printing assay may be the negative regulatory elements of $\beta$-lactoalobulin that serve tissue-specific repression.

• Korean Journal of Environment and Ecology
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• v.7 no.2
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• pp.231-240
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• 1994

The purpose of this study is to suggest the objective basic data for applying to development and conservation management of the national park through the quantitative analysis of the visual quality included in the and physical environment of the Tokyusan National Park. For this, spatial images and structures, of natural elements have been analyzed by factor analysis algorithm, and degree of visual quality has been measured mainly through questionnaries. Result of this study can be summarized as follows. Factors covering the spatial image of the Tokyusan National Park landscape have been found to be the overall synthetic evaluation, appeal, spatial, natural quality and dignity factors such as the overall synthetic evaluation, spatial and appeal yield high factor scores. The main factors determining the degree of visual quality are the clearness valley, peculiarity of configuration, natural of trail, harmony of suitable artificial planting and temple.

• The Journal of Engineering Geology
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• v.28 no.3
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• pp.465-473
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• 2018

The application of singular value decomposition (SVD) filtering is examined for attenuation of the ground-roll in land seismic data. Prior to the SVD computation to seek singular values containing the highly correlatable reflection energy, processing steps such as automatic gain control, elevation and refraction statics, NMO correction, and residual statics are performed to enhance the horizontal correlationships and continuities of reflections. Optimal parameters of SVD filtering are effectively chosen with diagnostic display of inverse NMO (INMO) corrected CSP (common shot point) gather. On the field data with dispersion of ground-roll overwhelmed, continuities of reflection events are much improved by SVD filtering than f-k filtering by eliminating the ground-roll with preserving the low-frequency reflections. This is well explained in the average amplitude spectra of the f-k and SVD filtered data. The reflectors including horizontal layer of the reservoir are much clearer on the stack section, with laminated events by SVD filtering and subsequent processing steps of spiking deconvolution and time-variant spectral whitening.

• Proceedings of the KSAR Conference
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• 2003.06a
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• pp.85-85
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• 2003

한우 난소의 황체는 다양한 세포들로 구성되어 있으며 난소의 생식기능유지와 임신유지에 중요한 인자가 복잡하게 관련되어 있으며 이들 황체에서 분비하는 단백질은 황체기능에 필수적으로 중요한 작용을 한다. 본 연구는 난소의 황체일령에 따른 단백질 분비 패턴을 조사함으로써 황체세포의 기능과 임신 유지에 관련되는 인자들을 조사하기 위하여 수행하였다. 한우에서 채취한 난소에서 황체를 분리, 황체시기별(전기, 중기, 말기)로 구분하여 cytosol을 분리 정제하였다. 황체시기별로 분리된 황체 단백질의 성분을 분석하기 위해 ion-exchange chromatography를 이용하여 단백질 패턴을 조사, 추출된 fraction을 단백질 정량후 SDS-PAGE를 실시하였다. 그 결과 중기에서의 단백질의 농도가 가장 높았으며, 특히 단백질 패턴 또한 다른 양상을 보였다. 시기별로 구분한 각각의 fraction을 SDS-PAGE로 조사했을 때 중기와 말기황체 cytosol의 fraction 3번과 4번에서 다른 양상을 보였으며 SDS-PAGE에서 120kb, 95kb, 34kb, 25kb 등의 단백질 밴드를 확인할 수 있었다. 초, 중기황체에서만 특이하게 검출되는 단백질 band를 확인할 수 있었으며 이 들 단백질을 구체적으로 확인하기 위하여 Two-Dimensional electrophoresis를 이용하여 실험을 수행하였다. 시기별로 분리된 황체를 일반적인 IEF단백질 분리법으로 cytosol을 회수한 후 IPG-system을 이용하여 1차원 전기영동을 한 후, SDS-PAGE로 이차원 전기영동을 실시하였다. 이차원 전기영동 결과, SDS-PAGE의 결과와 비슷한 위치에 부분적으로 다른 spot의 양상을 보였다. 특히 기능황체에서의 특이적 발현 spot을 확인할 수 있었다. 이러한 결과들로부터 황체의 progesterone분비기능의 역할을 수행하기 위한 단백질들이 전, 중기에 발현된다는 것을 알 수 있고 퇴행황체에서는 발현이 안되고 있는 것을 알 수 있다. 이러한 결과를 토대로 다른 양상을 띤 spot을 분리하여 어떤 단백질인지를 분석하여 각각의 황체단백질의 특성을 규명할 수 있을 것으로 사료된다.

• Parasites, Hosts and Diseases
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• v.32 no.4
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• pp.231-242
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• 1994

Pnragonimus westermnni, the lung fluke, is known to migrate to the pulmonary tissue of mammalian hosts and causes pathological changes in the lungs. An acidic thiol-dependent proteinase with a molecular weight of approximately 20,000 daltons was purified to homogeneity using ion-exchange chromatography and gel filtration chromatography. On SDS-PAGE, the molecular weight of the enzyme was 17,500 daltons. Isoelectric point was 6.45. The enzyme was similar to the acidic cysteine proteinase of vertebrates in the properties of pH optimum, substrate specificity and inhibitor sensitivity. Enzymatic activity was stable at pH 5.5 for at least two days when stored at 4℃. The cysteine proteinase was capable of degrading collagen and hemoglobin. Sera of patients with paragonimiasis and mice infected with R westermani reacted in immunoblots with the partially purified proteinase. This result suggested that the cysteine proteinase of P. westermnni may play a role in migration in tissues, and in acquisition of nutrients by parasites from the host. It is also potentially an antigen for the serodiagnosis of paragonimiasis.

• Tuberculosis and Respiratory Diseases
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• v.48 no.6
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• pp.879-886
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• 2000

Background : Thyroid Transcription Factor-1(TTF-1) acts as a tissue specific transcription factor in the regulation of lung specific gene expression and as morphogenic protein during lung organogenesis. Currently, there is very little information on the cis-acting sequences and transcription factors that direct the TTF-1 gene expression. DNAse 1 hypersensitive (DH) sites represent a marker for active or potentially active chromatin and are likely to be especially important in gene regulation, being associated with many DNA sequences that regulate gene expression. It is clear that DH regions correlate with genetic regulatory loci and binding for sequence-specific DNA-binding proteins. Methods : We have used DH site assays to identify putative distal regulatory elements in H441 lung adenocarcinoma cells, which express the TTF-1 gene and HeLa cells. Results : There are four DH sites 5' of the TTF-1 gene. These sites are located at base pair approximately +150, -450, -800, and -1500 from the start of transcription. Conclusion : These data suggest that there may be at least one intragenic site and regulatory region 5' prime to the promotor region.

• The Journal of Natural Sciences
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• v.14 no.1
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• pp.51-61
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• 2004

T7 RNA polymerase is a single subunit RNA polymerase able to accomplish whole transcription process without auxiliary factors. T7 phage lysozyme involcing in destruction of host cell wall repress T7 transcription and affects transcription termination process. Therefore expression vector pT7lys containing T7 phage lysozyme gene was constructed and expressed. T7 phage lysozyme protein was purified to homogeneity by Ni-NTA column chromatography. Also amidase activity of the purified lysozyme was identified. In order to understand the effect of the lysozyme on the sequence specific transcription termination. T7 transcription elongation complexes at the site rrnB T1 transcription termination signal were made in the presence the lysozyme. The results shows that the transcription elongation complexes are unstable in the presence of T7 phage lysozyme.

• Journal of Life Science
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• v.26 no.7
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• pp.855-867
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• 2016

The horse is relatively earlier domesticated animal species. Domesticated horses have been selected for their ability of racing, robustness, and disease-resistance. As a result, the thoroughbred horse genome has been condensed many genotypes related to exercise ability. In recent years, with the advent of NGS technologies, many studies were concentrated on finding superior genetic species in the horse genome in terms of genomics. Consequently, GWAS (Genome-wide Association study) is applied to horse genome, then genetic marker is revealed for superior racing ability. In addition, RNA-Seq is utilized as a method for analyze of whole transcript profiling in specific samples. By using this approach, specific gene expression patterns and transcript sequences can be revealed in various samples such as each individual, before and after exercise state, and each tissue. DNA methylation, a strong factor that regulate gene expression without the change of DNA sequence, have got a lot of attention. In horse genome, exercise- or individual-specific DNA methylation patterns were detected, and could be useful to develop selective marker of superior horses. MicroRNAs inhibit gene expression, and transposable elements accounted for half of the mammalian genome. These two elements are the crucial factors in functional genomics, and could be applied to the selection of superior horses. As the functional genomics and epigenomics advance, then these technologies introduced in this paper were applied to select superior horses. In this paper, the studies for selection of superior horses through genetic technologies, and development possibilities of these studies were discussed.