• Title/Summary/Keyword: 통성혐기성 세균

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The Aerobic Nature of Arcobacter nitrofigilis (Arcobacter nitrofigilis의 호기적 특성)

  • Han, Yeong-Hwan
    • Korean Journal of Microbiology
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    • v.32 no.1
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    • pp.7-11
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    • 1994
  • The free-livein nitrogen fixing bacterium, Arcobacter nitrofigilis which has been known to be a microaerophile, exhibited aerobic growth in brucella broth. In a level of oxygen equivalent to an air atmosphere (21% $O_2$), the maximum cell growth was observed in brucella broth. Low level of cell growth occurred in a level of low oxygen equivalent to lower than 2%, unless any other terminal electron acceptors other than oxygen were wupplied in brucella broth. Membrane-bound cytochrome b and c, and soluble cytochrome c were found. The growth in an aerobic atmosphere, little growth at low oxygen level, and occurrence of cytochrome c mean that this species is an aerobe and obtains energy using energy-yielding respiration.

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Microbial hydrogen production: Dark Anaerobic Fermentation and Photo-biological Process (미생물에 의한 수소생산: Dark Anaerobic Fermentation and Photo-biological Process)

  • Kim, Mi-Sun;Baek, Jin-Sook
    • KSBB Journal
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    • v.20 no.6
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    • pp.393-400
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    • 2005
  • Hydrogen($H_2$) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological $H_2$ production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological $H_2$ production processes, that are being explored in fundamental and applied research, are reviewed.

Seasonal distribution of microorganisms in the effluent water in various sewage treatment plants (하수처리장 방류수의 계절별 미생물분포에 관한 연구)

  • Kim, Young-Jun;Chung, Myung-Hee;Chung, Doo-Young;Lee, Hye-Young
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.1
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    • pp.53-61
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    • 2008
  • Seasonal distribution of various microorganisms was investigated in the effluent water in various sewage treatment plants from January to November in 2007. The target microorganisms were composed of total heterotrophic bacteria, Escherichia coli, coliform and enteric bacteria, Staphylococcus aureus, Yeast and Mold. The number of total bacteria and the indicator microorganisms, such as E. coli, coliform, and Enterobacteriacea, were increased during the summer season. S. aureus, however, was not influenced by the weather, showing higher number even in November and January in most sewage treatment plants investigated. Meanwhile, disinfection methods employed in various plants had no direct relationship with the growth of microorganisms, and small-scaled plants were found to be more effective than large-scaled ones in the sterilization of microorganisms.

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Complete genome sequence of Streptococcus gordonii KCOM 1506 isolated from a human acute pulpitis lesion (사람 급성치수염에서 분리된 Streptococcus gordonii KCOM 1506의 유전체 염기서열 해독)

  • Park, Soon-Nang;Roh, Hanseong;Lim, Yun Kyong;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.53 no.2
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    • pp.129-130
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    • 2017
  • Streptococcus gordonii is a Gram-positive, facultative anaerobic and non-motile cocci. S. gordonii is a member of oral flora and a pioneer species that initiate the dental biofilm formation. S. gordonii has also been implicated in the pulpitis of primary teeth as well as systemic diseases such as infective endocarditis and septic arthritis. S. gordonii is associated with oral, respiratory, and gastrointestinal tract infections. S. gordonii KCOM 1506 (= ChDC B679) was isolated from a human acute pulpitis lesion. Here, we present the complete genome sequence of S. gordonii KCOM 1506.

Food Waste Composting by Using an Inoculum-Mixture Containing New Facultative Anaerobic Bacteria (신규 통성혐기성 세균으로 제조한 발효흙에 의한 음식물 쓰레기의 퇴비화)

  • Hwang, Kyo-Yeol;Lee, Jae-Yeon;Kim, Keun;Sung, Su-Il;Han, Sung-Ho
    • Journal of the Korea Organic Resources Recycling Association
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    • v.9 no.1
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    • pp.65-72
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    • 2001
  • Four newly isolated bacteria from soil were used to manufacture microbial inoculum to compost food waste. The bacteria, GM103, V25, V31, and V35, were identified as Bacillus licheniformis, B. subtilis, B. stearothermophilius, and B, subtilis, respectively. The bacterial strains were efficient to degrade protein and starch and also able to inhibit the growth of plant pathogenic fungus Rhizopus stronifer. The GM103 showed distinct capability in degrading starch, but grow only aerobically. The other three bacterial strains. V25, V31, and V35, could grow both aerobically as well as anaerobically, in 10%(w/v) salt, at $50^{\circ}C$, and had good viability and survival rate in soil. These characteristics of the bacterial strains are very adquate in Korean food composting containing high concentration of salt, especially at home. By mixing the 4 bacterial culture broth with molasses, beet pulp, zeolite, The bacterial inoculum for food waste composting-BIOTOP-CLEAN-was made. The performance of food waste composting by the BIOTOP-CLEAN was compared with that by control(not treated) and HS(other demestic company's inoculum product for food waste composting). The maximum temperature of the food waste during the composting with the BIOTOP-CLEAN was $50^{\circ}C$, while those of the control and HS were $30^{\circ}C$ and $35^{\circ}C$, respectively. The BIOTOP-CLEAN gave the good smell and showed dark brown color, while the control gave bad smell and HS gave less bad smell. These indicates that the food waste composting by the BIOTOP-CLEAN had been well accomplished. The culture broth of V25, V31, V35 were sparyed to the plants of tomato, chinese cabbage, raddish, red pepper every month and the spraying the culture broth to these plant significantly improved the production yield of the crops, due to the control effect of the bacterial strains against the plant pathogens.

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Complete genome sequence of Eikenella corrodens KCOM 3110 isolated from human subgingival dental plaque of periodontitis lesion (사람 치주염 병소의 치은연하치면세균막에서 분리된 Eikenella corrodens KCOM 3110의 유전체 염기서열 완전 해독)

  • Lim, Yun Kyong;Park, Soon-Nang;Shin, Ja Young;Roh, Hanseong;Ji, Suk;Kook, Joong-Ki
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.154-156
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    • 2019
  • Eikenella corrodens is Gram-negative, facultatively anaerobic, and rod-shaped bacterium. It is a part of the normal human mucosal flora that can cause several systemic diseases such as endocarditis, liver abscess, and intracranial bacterial infection. E. corrodens KCOM 3110 (= JS217) was isolated from human subgingival dental plaque of periodontitis lesion. Here, we present the complete genome sequence of E. corrodens KCOM 3110.

Characterization of Enzymes Against Oxygen Derivatives Produced by Rhodobacter sphaeroides D-230 (Rhodobacter sphaeroides D230이 생성하는 산소 유도체에 작용하는 효소의 특성)

  • 김동식;이혜주
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.94-99
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    • 2004
  • The activities of enzymes that act on oxygen derivatives in Rhodobacter sphaeroides D-230 were investigated under various culture conditions. Intracellular SOD activity from the cells grown in aerobic or anaerobic culture conditions was highest at pH 7.0 and pH 8.0, respectively. On the other hand, extracellular SOD activity was highest at pH 6.0. Catalase activity was highest at neutral pH in both cases. Growth of R. sphaeroides D-230 in aerobic or anaerobic culture conditions was inhibited by methyl viologen. As R. sphaeroides D-230 was cul-tured aerobically, SOD activity was increased about 2-fold by addition of iron ion. But $Mn^+2$ had little effect on the SOD activity of R. sphaeroides D-230 grown in aerobically. NaCN, the inhibitor of Cu$.$Zn-SOD, did not inhibit SOD activity. But, $NaN_3$, the inhibitor of Mn-SOD, inhibited SOD activity in anaerobic cultures con-dition. Therefore, R. sphaeroides D-230 produce Mn-SOD in anaerobic condition, although Fe-Sod is produced in aerobic condition. The activity of catalase was induced by methyl viologen, however, extremely inhibited by NaCN and $NaN_3$.

Isolation and Identification of Enterococcus faecalis 2B4-1 Containing Antitumor Substances. (항종양활성을 지닌 Enterococcus faecalis 2B4-1의 분리 및 동정)

  • 박상진;임대석;윤상군;백영진;김창한
    • Microbiology and Biotechnology Letters
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    • v.26 no.6
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    • pp.471-475
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    • 1998
  • The aim of the present research program was to develop a strain of gastrointestinal bacteria containing antitumor substances. Fecal samples were collected from neonates and a number of gastrointestinal bacteria were isolated from the fecal samples by applying selective agar for intestinal bacteria. Among 127 isolates, a strain 2B4-1 containing an antitumor substance against stomach cancer, SNU-1, was selected. The strain 2B4-1 was identified as a strain similar to Enterococcus faecalis NCTC 775 with respect to morphological characteristics, growth temperature, salt and acid tolerance, growth under facultative anaerobic conditions and utilization of carbon sources such as arabinose and melibiose and so on. However, it showed some differences such as a negative reaction to hippurate hydrolysis and negative reaction to $\beta$-hemolysis. We assigned to the strain 2B4-1 to Enterococcus faecalis.

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Biological Probiotic Properties of Lactobacillus rhamonosus GG-4 Isolated from Infant Feces (유아분변으로부터 분리한 Lactobacillus rhamonosus GG-4의 생균제적 특성)

  • Kang, Jin-Hae;Kim, Dae-Hwan;Lee, Sang-Won;Kim, Hong-Chul;Cho, Young-Un;Gal, Sang-Wan
    • Journal of Life Science
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    • v.20 no.12
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    • pp.1882-1888
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    • 2010
  • To develop probiotics, a kind of Lactobacillus sp. was isolated from infant feces. The bacterium was identified as Lactobacillus rhamnosus through 16S rDNA sequence analysis. The strain was a facultative anaerobe which grew better in aerobic conditions. The bacterium lowered the pH of the culture solution down to 2.4 during 48 hr in the MRS medium. The strain inhibited the growth of 6 pathogens - S. aureus, L. monocytogens, S. typhimurium, E. coli O-157, V. parahaemolyticus and P. aeruginosa. When the Lactobacillus were fed to chickens, along with commercial feed, for one month, amounts of $H_2S$ and $NH_3$ in the feces of the chicken decreased to 50% and 70%, respectively, compared to those of control group chickens. Amounts of other bad smells such as $(CH_3)SH$, $(CH_3)_2S$ and $(CH_3)_2S_2$ were not much different in the Lactobacillus-fed chickens compared to the control group. On the other hand, egg weights of the chickens fed Lactobacillus were higher by about $5{\pm}1\;g$ than those in the control group.

Antioxidant Activity and Characterization of Exiguobacterium sp. SC2-1 Isolated from Sea Water (해양에서 분리한 Exiguobacterium sp. SC2-1의 항산화 활성 및 특성)

  • Kim Man-Chul;Park Guen- Tae;Son Hong-Joo;Choi WooBong;Heo Moon-Soo
    • Korean Journal of Microbiology
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    • v.41 no.1
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    • pp.74-80
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    • 2005
  • For the reseach of the natural marine antioxidant, an antioxidant-producing bacterium was isolated from seawater in Jeju costal area. The isolated strain SC2-1 was Gram-positive, catalase positive, oxidase negative, motile and small rods. The strain utilized sucrose, dextrose, fructose, mannitol and maltose as a sole carbon and energy source and NaCl required for growth. The radical scavenging activity of the culture supernatant was detemined by DPPH method. This bacterium was identified based on morphological, biochemical characteristics and 16S rDNA sequence analysis, and then was named Exiguobacterium sp. SC2-1. The optimum conditions of culture for production antioxidnat were $25^{\circ}C$, pH 6-8 and $4\%$ NaCl. The stain showed the highest activity and growth cultured in medium which added $1\%$ maltose. Hydroxyl radical activity of the supernatant of Exiguobacterium sp. SC2-1 was $73\%$. The SOD activity of the culture supernatant was estimated about $35\%$.