• Korean Journal of Microbiology
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• v.44 no.1
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• pp.74-79
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• 2008

The purpose of this study is to clone cgt gene from Paenibacillus sp. JB-13 and to overexpress the protein in E. coli. For this purpose, the cgt gene was amplified from Paenibacillus sp. JB-13 genomic DNA by PCR using degenerate oligonucleotide primers. The sequence analysis results showed that the cgt gene from Paenibacillus sp. JB-13 has 98% homology with the cgt gene of Bacillus sp. To overexpress the protein, the cgt gene was cloned into pEXP7 expression vector and transformed into E. coli. The production of CGTase by recombinant E. coli was optimized under following conditions: 0.5% glucose, 3.0% polypeptone, 0.3% $K_2HPO_4$, 0.5% NaCl, and 7.0 of initial pH, 2.0% of inoculum, $37^{\circ}C$ of culture temperature for 14 hr. And the optimal agitation was found at 0.1 vvm. The synthesis of 2-O-${\alpha}$-D-Glucopyranosyl L-Ascorbic acid (AA-2G) using the CGTase expressed in E. coli was identified as AA-2G by HPLC and HPLC confirmed that treating AA-2G made by cloned CGTase with ${\alpha}$-glucosidase substantially produced AA and glucose.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.20 no.2
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• pp.29-33
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• 2014

Active air flushing mini-pumps were installed in a rigid polypropylene container ($32cm{\time}23cm{\time}18cm$) containing 900 g of fresh green peppers for effectively controlling its $O_2$ concentration on real time basis to preserve the product quality. The performance of the constructed system was compared to that of the modified atmosphere (MA) container system with gas diffusion tube controlled in close/open cycles responding to real time $O_2$ concentration at 10 and $20^{\circ}C$. In the control logic, the $O_2$ concentration was programmed to be located exactly at 13% or stay in the range of 13-15%. The active air flushing system could control the $O_2$ concentration in the desired level or range at both temperatures, while the passive diffusion system could work only under the low temperature condition of $10^{\circ}C$. At higher temperature of $20^{\circ}C$, the passive diffusion system could not manage the produce respiration increased more highly than the gas transfer through the diffusion tube, resulting in too low $O_2$ concentration and too high $CO_2$ concentration which would be injurious to the green pepper. When tested at $20^{\circ}C$, the MA container system could preserve the green pepper better than the perforated air package in terms of weight loss, ascorbic acid and chlorophyll contents and firmness.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1140-1145
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• 1998

Low density polyethylene (LDPE) films incorporated with 1% antimicrobial agents of Rheum palmatum extract, Coptis chinensis extract, and Ag-substituted inorganic zirconium matrix, were applied to modified atmosphere packaging of 200 g fresh strawberries. Plain LDPE film package, PVC wrap and perforative pinhole package of the film impregnated with 1% Rheum palmatum extract were also constructed for comparative purpose. All the packages were stored for 13 days at $5^{\circ}C$ and measured in package atmosphere, microbial count and quality attributes of the strawberry fruits. The antimicrobial LDPE films retarded the growth in total aerobic bacteria, lactic acid bacteria and yeast on the fruits, and resulted in significantly lower decay. The degrees of reduced microbial growth and fruit decay in the antimicrobial film packages were more pronounced, when applied by hermetical sealing to produce the modified atmospheres of low $O_2$ (<4.0%) and $CO_2$ concentrations with $6.3{\sim}9.0%$. The hermetically sealed packages of antimicrobial LDPE films also showed better retention of fruit firmness and did not give any negative effect on the physical and chemical qualities of strawberries.

• Journal of Food Hygiene and Safety
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• v.17 no.4
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• pp.183-187
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• 2002

This study was performed to investigate the utility of egg-shell calcium with calcium soak egg-shell in the vinegar. We compared examination eluted quantity with ionization rate in each condition. The results were as follows. 1 The degree of elution and ionization of calcium was proportional to the amount of vinegar and added acetic acid in the vinegar for elution. And ionization rate was the most high in case of 200 ml vinegar volume. 2. fluted quantity were increased in the rank order to 4$0^{\circ}C$ > 3$0^{\circ}C$ > 2$0^{\circ}C$ by temperature but the rank order of ionization of calcium was 3$0^{\circ}C$ > 2$0^{\circ}C$ > 4$0^{\circ}C$ by temperature. The occurrence of unacceptable flavor resulting from the immersion at 4$0^{\circ}C$ was another obstacle to adopt. 3. Eluted quantity and ionization rate of the egg-shell calcium were appeared excellent in case of the brewage vinegar at ventilation condition. The case of brown rice vinegar did not show a considerable difference, but eluted quantity and ionization rate were appeared the most excellent to the other sample. 4. The optimum condition for elution and ionization of the egg-shell calcium was appeared Immersing egg-shells in the brewed rice-bran vinegar at 3$0^{\circ}C$ for three days.

• Journal of Animal Environmental Science
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• v.15 no.1
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• pp.69-74
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• 2009

A survey questionnaire that addressed the issues to manure compost producers and users was prepared. The questionnaire addressed three main topics as follows: 1) types of manure treatment and composting facilities are being operated, 2) quantity of manure compost produced and used, 3) problems experienced in producing and using manure compost. A total of 30 manure compost producers and 10 manure compost users were interviewed. Solid manure are applied to composting. Slurry and wastewater are simplified aeration method to produce liquid fertilizer. The open elongated type manure composting are generally used on manure compost centers jointly used by several farms. The amount of annually manure compost production was most common in the range of $5{\sim}10$ thousand tons per manure compost center. The manure compost utilization and cucumber yield were mostly $6{\sim}15$ tons and $20{\sim}27$ tons per 10a of cucumber farmland, respectively. Environmentally friendly use both of manure compost and chemical fertilizer are recommended for natural recycling agriculture.

• Horticultural Science & Technology
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• v.18 no.4
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• pp.529-535
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• 2000

Organic materials such as composted rice-hull, saw dust, and pine bark, and inorganic materials such as vermiculite, perlite, and recycled rockwool were commonly employed as container media in domestic greenhouse industry. The objective of this research was to get informations in soil physico chemical properties of those materials. Composted dry-peeling bark and wet-peeling bark had 72.1% and 69.1%, respectively, in particles larger than 1.0 mm, which were much higher than 34.7% of composted rice-hull and 33.7% of composted saw dust. Imported vermiculite had 89.9%, but domestic vermiculite had 25.7% in particles larger than 1 mm. In soil physical properties, Russian peat had the highest container capacity of 79.3%, and wet-peeling bark had the lowest container capacity of 58.2%. However, Russian peat and composted saw-dust had 4.1% in air space indicating that possible problems could occur in soil aeration when those are employed for container grown crops. Saw dust had $2.3mS{\cdot}cm^{-1}$ in electrical conductivity, while other composted organic materials had less than $0.25mS{\cdot}cm^{-1}$. Imported vermiculite had 64.0 meq/100 g in cation exchange capacity, which was 2.4 times higher than those of domestic vermiculite, 27.2 meq/100 g. Domestic vermiculite had higher Ca and Mg and less Na contents than those of imported vermiculite.

• Journal of Bio-Environment Control
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• v.11 no.4
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• pp.175-180
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• 2002

This study was conducted to examine the effect of row covering materials on the growth and yield of broccoli for early production in the open meld. Materials for plastic tunnel were row-covering (ventilating non-woven fabric), perforated film (2.3％ pore space) and P.E. film. The types of row covers were tunnel and covering period was one months after transplanting. The row cover and perforated film increased daily air and soil temperature by 3~4$^{\circ}C$ and 2~3$^{\circ}C$, respectively as compared to no covering. Also, relative humidities in those treatments were higher than those in P.E. and no covering. The row covering and perforated film enhanced plant growth. Broccoli grew best and abnormal head appeared the least with row covering treatment. Days taken for head formation, however, did not differ between treatments but the weight of curd was over 400 g in the treatment of row covering and perforated film covering. Marketable yield doubled under row covering as compared to no covering. The first harvest time was earlier by two weeks in row-covering. The results of this experiment indicated that covering row-cover film in spring cultivation was effective fer not only yield increase but also quality evaluation in early production of broccoli.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.2 no.3
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• pp.18-24
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• 1999

This study was aimed to develope appropriate soil media for the growth of Rhododendron hybrid $J_{ASANHONG}$ on the artificial ground five types of soil media was tested such as "sandy loam-general soil (T1)", "vermiculite-artificial soil (T5)", "sandy loam 50% + vermiculite 30% + sand 20% (improved of soil 2-T2)", "sandy loam 50% + carbonized rice hust 30% + sand 20% (improved of soil 3-T3)", "sandy loam 50% + humus sawdust 30% + sand 20% (improved of soil 4-T4)". The result of the research are as follows. 1. Among the type of soil media, the sandy loam(T1) soil type gave the worst effects on growth of above ground parts(height, No. of leaf, width of leaf, No. of flowering, dry weight of upper parts) and under ground parts(dry weight of roots). 2. Vermiculite(T5) showed the highest root growth(dry weight of roots). it seemed to be caused high saturated hydraulic conductivity and porosity. As a result, there is much available space for enabling the root spreads. 3. "sandy loam 50% + vermiculite 30% + sand 20%(improved of soil 2-T2)", "sandy loam 50% + carbonized rice hust 30% + sand 20%(improved of soil 3-T3)" showed good effects on growth of above ground parts and under ground parts compared with sandy 10am(T1) 4. "sandy loam 50% + humus sawdust 30% + sand 20% + (improved of soil 4-T4)" showed the highest effects on growth of above ground parts.

• Journal of the Korean Geotechnical Society
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• v.20 no.3
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• pp.23-31
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• 2004

Normally, coating is used for protecting reinforced concrete. For this purpose, both organic and inorganic coatings are used. The advantages of inorganic coatings are lower absorption of UV, non-burning etc. On the other hand, organic coatings have the advantage of low permeability of $CO_2, SO_2$ and water. Organic coatings provide better protection for reinforced concrete. However, organic coatings such as epoxy, urethane and acryl reduce long-term adhesive strength by the difference of their thermal expansion coefficients and elastic modules from those of concrete, and the formed coating cover of these is blistered by poor breathing. Also, when organic coatings are applied to the wet surface of concrete, they have a problem with adhesion. In this study, a new coating material for protecting concrete was hybridized with polymer and ceramics. And tests were carried out on its physical and durable characteristics, and safety characteristic on elution. All results were compared with organic coating materials and epoxies and showed that the performance of the developed coating material was not inferior to that of other organic coatings in protecting concrete. On the other hand, safety characteristic on elution was superior to epoxies which were used in this study. So, the developed coating material was considered as a suitable protecting coating material which have advantages of inorganic and organic coatings for protecting underground concrete structures, especially in contact with water.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.23 no.7
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• pp.1797-1806
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• 1998

Future satellite communication systems will be developed at Ka-band (20/30 GHz) owing to the relatively wide frequency allocation and current freedom from terrestrial interference for multimedia services. A serious disadvantage of the Ka-band, however, is the very high atmospheric attenuation in rainy weather. Synchronous CDMA drastically redces the effect of self-noise with several interesting features of CDMA for mobile communications such as fixible freuqncy rese, the capability of performin soft-handover and a lower sensitivity to interference. This paper evaluates the performance of a synchronous CDMA reture link for a Ka-band geostationary satellite communication system. For a fixed satellite channel whose characteristics depend on weather conditions, the signal envelope and phase for this channel is modelled as Gaussian. The bit error and outage probability, and the detection loss due to imperfect chip timing synchronization is analytically evaluated and the system capacity degaradation due to the weather condition is estimated. The two cases consist of the general case in which all users are affected by rain condition, and the worst case in which the reference user is only affected by rain attenuation. the results for two cases of rain condition clearly show that synchronous CDMA eases the power control requirements and has less sensitivity to imperfect power control.