• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.2
• /
• pp.221-227
• /
• 2011

Applications of green manures generally improve the soil quality in rice paddy in part through restructuring of soil microbial communities. To determine how different green manures affect soil microbial communities during the early stages of rice growth, fatty acid methyl ester (FAME) profiles were used to the effects of different management practices: 1) conventional farming (CF), 2) no-treatment (NT), 3) Chinese milk vetch (CMV), 4) green barley (GB), and 5) triticale in paddy field. With applications of green manures, soil organic matter was significantly higher than CF, while soil Na concentration was significantly lower compared with CF (p<0.05). Total soil microbial biomass of CMV was higher (p<0.05) than NF by approximately 31%. The highest ratio of monounsaturated fatty acid to saturated fatty acid was found in the GB plot, followed by CMV and triticale compared with CF (p<0.05), possibly indicating that microbial stress was less in GB and CMV plots. Populations of Gram-negative bacteria and arbuscular mycorrhizal fungi also were significantly higher green manures than CF (p<0.05). Our findings suggest that GB should be considered as optimum green manure for enhancing soil microbial community at an early growing stage in paddy field.

• Korean Journal of Agricultural Science
• /
• v.16 no.2
• /
• pp.152-162
• /
• 1989

Oligotrophic bacteria studied in the present report were isolated on a 100-fold dilution of the nutrient broth(NB) medium and were able to grow on a 10,000-fold dilution of the NB medium. Oligotrophbic bacteria were shown to exist abundantly in all layers of a rendzina forest soil through the year. Two-hundred three oligotrophic bacteria were isolated from forest soil at different layers(L.F.H and A layers), and their morphological and physiological characteristics were examined. They were divided into 4 groups on their cell shape: (1) regular rods(Group I. 73 isolates), (2) curved/spiral rods (Group II, 29 isolates), (3) irregular rods (Group III, 56 isolateds), (4) append aged organisms (Group IV, 45 isolates), A high propoltion(95%)of the isolated oligotrophs were Gram-negative. all the isolated were aerobic, nonspore-formers. Forty isolates utilized fenolic acid such as ferulic acid or p-coumaric acid. sixty-for isolateds utilized C-1 compounds such as methanol or formic acid.

• Korean Journal of Microbiology
• /
• v.28 no.3
• /
• pp.199-203
• /
• 1990

To study distribution of bacterial flora and their biochemical characteristics in the Antarctic soilecosystem, these experiments were performed during the austral summer(Feb., 1989) on the King George Island, Antarctica. The numbers of heterotrophic bacterial colonies and extracellular enzyme actibities were estimated from the Antarctic terrestrial soils which were sampled from 17 different locations near Sejong station (Korea) and Teniente Jubany station (Argentina) on the King George Island. The numbers of heterotrophic bacterial colonies were extremely variable with sampling sites and incubation temperatures. Arithmetric average numbers were $2.5\times 10^{4}$, $2.7\times 10^{7}$ , $6.9\times 10^{5}$ CFU/$cm^{3}$ soil at the incubation temperature of $37^{\circ}C$, $25^{\circ}C$ and $4^{\circ}C$, respectively. The activities of extracellular $\alpha$-glucosidase, $\beta$-glucosidase and N-acetyl-$\beta$-glucosaminidase were shown as similar mean percentage in the colonies obtained at different temperatures. Mean value of protease activities, however, was remarkably higher (92%) in the colonies grown at $4^{\circ}C$,.

• The Bimonthly Magazine for Agrochemicals and Plant Protection
• /
• v.13 no.3 s.108
• /
• pp.65-71
• /
• 1992

• Proceedings of the Korean Society of Soil Zoology Conference
• /
• 1998.10a
• /
• pp.15-15
• /
• 1998

• Korean Journal of Soil Science and Fertilizer
• /
• v.17 no.3
• /
• pp.299-306
• /
• 1984

The influences of applications of organochlorine insecticide (HCH: Hexachlorocyclohexane, 10 ppm), fungicide (TPN: Tetrachloroisophthalonitrile, 40 ppm) and manure ($3Kg/m^2$) each or together on changes in soil microflora for consecutive years were investigated in the experimental field plots. The insecticide had a little effect on soil microbial numbers. In particular, the number of total bacteria, Gram-negative bacteria and fungi were gradually increased at the latter stage of the consecutive application, but the number of sporeforming bacteria reduced. The fungicide reduced the counts of sporeforming bacteria, actinomycetes and fungi respectively, whereas increased prominently the counts of total bacteria and Gram-negative bacteria. TPN-resistant bacteria, particulary TPN-resistant Gram-negative bacteria were gradually accumulated by the long-term application of TPN, and further the number of TPN-resistant total bacteria and the of TPN-resistant Gram-negative bacteria correlated fairly well during all the period. The influences of combined applications of both HCH and TPN on the number of soil microorganisms were equal to the respective sums of the effects of single application of each pesticide. The combined application of manure and these pesticides elevated the increasing extents of microbial numbers, while weakened the detrimental efforts of these pesticides on microbial numbers. These data suggest that the long-term application of these materials have resulted in the remarkable changes of composition of soil microflora.

• Proceedings of the Korean Institute of Resources Recycling Conference
• /
• 2001.05a
• /
• pp.507-510
• /
• 2001

본 연구에서는 음식물 찌꺼기를 원료로 생균 발효사료를 생산하기 위해 단시간 호기적 발효에 필요한 고온성 균주를 개발하고자 하였다. 폐기물 처리장이나 경작연수가 10년 이상 경과된 농경지 야산의 토양 등 유기물질이 많은 장소에서 채취한 토양은 종균원으로 이용하였다. 1차 Screening을 통해 재배지(B group), 폐기물 처리장(F group), 야산(W group)에서 채취한 토양과 시판 발효용 종균제로부터 고온성 세균들을 분리한 뒤 고온.호기적 속성 발 효에 적합한 균주를 가려내기 위해 발효실험을 통한 2차 screening을 실시하였다. 그 결과, 재배지 토양으로부터 Bl, B2, B3, B4, B6, 폐기물 처리장으로부터 Fl, F2, 야산으로부터 W1, W3, W4 등의 세균이 선발되었다. 이들 중 특히 B6는 발효개시 4시간만에 생균수가 1.3$\times$$10^{9}$m1에 이르러 높은 중식속도를 나타냈고, 8시간만에 최고치인 7.5$\times$$10^{9}$ml까지 증가하여 호기적 고온성 속성 발효에 적합한 균주로 사료되었다.

• Korean Journal of Soil Science and Fertilizer
• /
• v.32 no.3
• /
• pp.312-318
• /
• 1999

These experiments were conducted to determine the effects of soils treated with different soil management, methods, fertilizers and crops on soil microflora and its number for constructing the environmentally sound agriculture modeling of the upland soils. These experiments show that physico-chemical soil characteristics was different in upland soils treated with different soil managements and fertilizers applied. Also soil microflora and its number were effected from crops cultivated with different soil managements in these experiments. The number of Erwinia sp. in chinese cabbage cultivated field on Daekwanryung especially was apparently increased in soil cultivated with potato relative to corn. It was appeared that the number of Fusarium sp. in soil was more effected from kind of fertilizer applied than crops cultivated.

• Korean Journal of Soil Science and Fertilizer
• /
• v.29 no.1
• /
• pp.53-59
• /
• 1996

This study was conducted to evaluate the effects of fowl dzopping. saw dust and rice hall on the soil microflora in vitro. The experiment was designed in seven treatments with the various organic materials and they were only soil (control). soil + fowl dropping (S+F), soil+fowl dropping+rice hull (S+F+R) soil+fowl dropping*saw dust (S+F+S). soil+chemical fertilizer (S+C.F), fowl dropping+rice hull (F+R) and fowl dropping+saw dust (F+S). All the samples of treatment were incubated in $28{\pm}2^{\circ}C$ condition and tested the activity of soil microflora for 84 days The activity of fungi, total bacteria, gram-negative bacteria and actinomycetes showed the highest values at, twenty-first day and the spore-forming bacteria was at forty-second day after incubation. The number of fungi and gram-negative bacteria showed the highest values in the treatment of F+S, the spore-forming bacteria and the actinomycetes were in the S+F+S. and the number of total bacteria was in the F+C.F., but in the treatment of F+R. all the microorganism except fungi showed the lowest values in their numbers. The composition ratio of dead bacteria was higher in the treatments of S+F+R and F+R than in those of others as 70% and 40% respectively. Actinomycetes isolated from the treatments of S+F and S+F+S were identified as Streptomyces sp.. Nocardia sp., Micromonospora sp. Actinomadura sp. and Saccharomonospora sp.

• Korean Journal Plant Pathology
• /
• v.12 no.4
• /
• pp.381-388
• /
• 1996

맥류세균성 줄무늬병균의 선택배양기(KM-1)를 개발하여 이병식물체 및 토양으로부터 Xanthomonas campestris pv. translucens를 선택적으로 분리할 수 있는 효율성을 검토하였다. KM-1배양기의 구성성분은 증류수 1 L당 lactose 10 g, D(+)trehalose 4.0 g, thiobarbituric acid 0.2 g, K\ulcornerHPO\ulcorner 및 KH\ulcornerPO\ulcorner 각각 0.8 g, yeast extract 30 mg, NH\ulcornerCl 1 g, cycloheximide 100 mg, tobramycin 8.0 mg, ampicillin 1.0 mg 및 Bacto agar 15 g이며 1 N NaOH로 pH 6.6으로 조절하였다. X. c. t.의 균주별 KM-1의 배양효율은 비선택성 농후배지인 Wilbrinks agar에 비하여 1.30정도였으며, 기타 토양전염성식물병원세균 Agrobacterium tumefaciens, Agrobacterium rhizogenes, Erwinia carotovora var. atroseptica, Erwinia carotovora var. carotovora, Corynebacterium insidiosum, 및 기타 토양생존 부생세균 Bacillus cereus, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas putida 등의 생장을 완벽하게 억제하였다. KM-1의 저장기간(shelf-life)도 5$^{\circ}C$에서 2개월 동안 선택성을 유지하였다. 따라서 본 병원균의 전염원의 생존 등 발생생태연구에 활용될 수 있는 가치가 충분히 인정되었다.