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Charaterization of Biomass Production and Wastewater Treatability by High-Lipid Algal Species under Municial Wastewater Condition (실제 하수조건에서 고지질 함량 조류자원의 생체생성과 하수처리 특성 분석)

  • Lee, Jang-Ho;Park, Joon-Hong
    • Journal of Korean Society of Environmental Engineers
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    • 제32권4호
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    • pp.333-340
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    • 2010
  • Wastewater treatment using algal communities and biodiesel production from wastewater-cultivated algal biomass is a promising green growth technology. In literature, there are many studies providing information on algal species producing high content of lipid. However, very little is known about adaptability and wastewater treatability of such high-lipid algal species. In this study, we attempted to characterize algal biomass production and wastewater treatability of high-lipid algal species under municipal wastewater condition. For this, four known high-lipid algal strains including Chlorella vulgaris AG 10032, Ankistrodesmus gracilis SAG 278-2, Scenedesmus quadricauda, and Botryococcus braunii UTEX 572 were individually inoculated into municipal wastewater where its indigenuous algal populations were removed prior to the inoculation, and the algae-inoculated wastewater was incubated in the presence of light source (80${\mu}E$) for 9 days in laboratory batch reactors. During the incubations, algal biomass production (dry weight) and the removals of dissolved organics (COD), nitrogen and phosphorous were measured in laboratory batch reactors. According to algal growth results, C. vulgaris, A. gracilis and S. quadricauda exhibited faster growth than indigenuous wastewater algal populations while B. braunii did not. The wastewater-growing strains exhibited efficient removals of total-N, ${NH_4}^+$-N, Total-P and ${PO_4}^{3-}$-P which satisfy the Korea water quality standards for effluent from municipal wastewater treatment plants. A. gracilis and S. quadricauda exhibited efficient and stable treatability of COD but C. vulgaris showed unstable treatability. Taken together with the results, A. gracilis and S. quadricauda were found to be suitable species for biomass production and wastewater treatment under municipal wastewater condition.

Quality of Surface Water for lrrigation around Controlled Horticultural Area in Gyeongnam (경남지방 시설원예지 농업용 지표수의 수질 현황)

  • Heo, Jong-Soo;Ha, Yeong-Rae;Seo, Jeoung-Yoon;Cho, Ju-Sik;Lee, Sung-Tae;Lee, Hong-Jae
    • Korean Journal of Environmental Agriculture
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    • 제16권4호
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    • pp.356-364
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    • 1997
  • To investigate the water quality status of agricultural water source for greenhouse area in Gyeongnam, the surface water quality was examined six times from October in 1995 to March in 1996 at five areas in Gyeongnam. The pH values of surface water were in the range of 6.6${\sim}$9.1 pH in Kimhae and Changnyong areas were out of range in 6.0${\sim}$8.5 which was water quality standard for agriculture. The DO values of surface water were relatively high with average 10.0mg/l in Kimhae, Changnyong, Sacheon and Chinju areas except for Haman area. The BOD values of surface water exceeded water quality standard for agriculture(8.0mg/l) in three sites and one site in Haman and Sacheon, respectively. The COD values of surface water exceeded water quality standard for agriculture(8.0mg/l) in Kimhae, Changnyong and Haman. The ${NH_4}^+-N$ values in surface water of Changnyong and Haman areas were 1.21mg/l and 2.75mg/l, respectively. The average values of $NO_3\;^--N$ in surface water was appropriate for agriculture. The values of $K^+,\;Na^+,\;Mg^{2+},\;Ca^{2+},\;{PO_4}^{3-}$ and $SO_4^{2-}$ in Haman were the highest of those of the others. And Pb was below 0.1mg/l which was water quality standard for agriculture. The average values of Cu, Cd and Zn were below water quality standard for agriculture. Between COD and SS in surface water was positively correlated with r$=0.799^{{\ast}{\ast}}$. BOD in surface water was positively correlated with $NH_4\;^+-N,\;PO_4\;^{3-},\;SS,\;K^+,\;Na^+$ and $Cl^-$. Surface water pollution status of agricultural water source of greenhouse areas in Gyeongnam was in order of Chinju< Sacheon< Kimhae< Changnyong< Haman area.

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Mineralization of Cattle Manure Compost at Various Soil Moisture Content (우분퇴비 시용후 토양수분 조절에 따른 질소 및 탄소의 전환)

  • Kim, P.J.;Chung, D.Y.;Chang, K.W.;Lee, B.L.
    • Korean Journal of Environmental Agriculture
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    • 제16권4호
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    • pp.295-303
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    • 1997
  • To investigate the transformation characteristics of nitrogen and carbon from cow manure compost amended in soil under different moisture conditions, dynamics of nitrogen and carbon were determined periodically for 15 weeks of aerobic incubation at room temperature during July${\sim}$November, 1996. Cow manure compost matured with mixing saw dust was amended with the 4 ratios (0, 2, 4, 6%(wt/wt)) in Ap horizon soil, which collected from green house in Yesan, Chungnam. Moisture was controlled with 0.2, 0.3, 0.4, and 0.5 of mass water conte nt (${\theta}$m) to air dried soil, and water loss was compensated at every sampling. During incubation, soil pH was decreased continuously, that was caused by hydrogen generated from nitrification of ammonium nitrogen. And pH became higher with inclining cow manure compost amendment and water treatment, that meaned the increase of mineralization of organic-N to $NH_4\;^+-N$. Total nitrogen was reduced with increasing water content, but total carbon showed the contrast tendency with that of nitrogen. Therefore, C/N ratio slightly decreased in the low water condition (${\theta}$m 0.2) during incubation, but increased continuously in high water condition over ${\theta}$m 0.4. As a result, it was assumed that soil fertility is able to be reduced in the high water content over available water content. Nitrate transformation rate increased lasting in the low water content less than ${\theta}$m 0.3. Itdropped significantly in the first $2{\sim}3$ weeks of incubation over ${\theta}$m 0.4. In particular, nitrate was not detected in ${\theta}$m 0.5 of water content after the first $2{\sim}3$ weeks. In contrast, ammonium transformation was inclined with increasing water treatment. Nitrogen mineralization rate, which calculated with percentage ratio of (the sum of ex.$NH_4\;^+-N$ and $NO_3\;^--N$)/total nitrogen, was continuously increased in the low water content of ${\theta}$m 0.2 and 0.3. But it saw the different patterns in high water content over ${\theta}$m 0.4 that was drastically declined in the initial stage and then gradually inclined . From the above results, nitrogen transformation patterns differentiated decisively in water content between ${\theta}$m 0.3 and 0.4 in soil. Thus, it is very important for the maintain of suitable soil water content to enhance fertility of soil amended with manure compost. However, excess treatment of manure compost might enhance the possibility of contamination of small watershed and ground water around agricultural area.

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Evaluation of Removal Efficiencies of Heavy Metals Using Brown Seaweed Biosorbent Under Different Biosorption Systems (폐미역을 이용한 생물흡착 시스템별 중금속 제거 효율 평가)

  • Choi, Ik-Won;Seo, Dong-Cheol;Kim, Sung-Un;Kang, Se-Won;Lee, Jun-Bae;Lim, Byung-Jin;Kang, Seok-Jin;Jeon, Weon-Tai;Heo, Jong-Soo;Cho, Ju-Sik
    • Korean Journal of Environmental Agriculture
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    • 제30권3호
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    • pp.310-315
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    • 2011
  • BACKGROUND: Heavy-metal pollution represents an important environmental problem due to the toxic effects of metals, and their accumulation throughout the food chain leads to serious ecological and health problems. METHODS AND RESULTS: Optimum conditions in continuous-flow stirred tank reactor (CSTR) and packedbed column contactor (PBCC) using brown seaweed biosorbent were investigated. Under optimum conditions from both lab-scale biosorbent systems, removal efficiency of copper (Cu) in a large-scale PBCC system was investigated. Removal capacity of Cu using brown seaweed biosorbent in a lab-scale CSTR system was higher than that in a lab-scale PBCC system. On the other hand, over 48 L/day of flow rate in Cu solution, removal efficiency of Cu in a lab-scale PBCC system was higher than that in a lab-scale CSTR system. Optimum flow rate of Cu was 24 L/day, optimum Cu solution concentration was 100 mg/L. Removal capacity of Cu at different stages was higher in the order of double column biosorption system > single column biosorption system. Under different heavy metals, removal capacities of heavy metal were higher in the order of Pb > Cr > Ni > Mn ${\geq}$ Cu ${\geq}$ Cd ${\fallingdotseq}$ Zn ${\geq}$ Co. Removal capacity of Cu was 138 L in a large-scale PBCC system. Removal capacity of Cu a large-scale PBCC system was similar with in a lab-scale PBCC system. CONCLUSION(s): Therefore, PBCC system using brown seaweed biosorbent was suitable for treating heavy metal wastewater.

Characteristics of Groundwater Quality for Agricultural Irrigation in Plastic Film House Using Multivariate Analysis (다변량분석법을 이용한 시설재배지 지하수 수질 특성)

  • Kim, Jin-Ho;Choi, Chul-Mann;Lee, Jong-Sik;Yun, Sun-Gang;Lee, Jung-Taek;Cho, Kwang-Rae;Lim, Su-Jung;Choi, Seung-Chul;Lee, Gyeong-Ja;Kwon, Yeu-Seok;Kyung, Ki-Chon;Uhm, Mi-Jeong;Kim, Hee-Kwon;Lee, You-Seok;Kim, Chan-Yong;Lee, Seong-Tae;Ryu, Jong-Su
    • Korean Journal of Environmental Agriculture
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    • 제27권1호
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    • pp.1-9
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    • 2008
  • The main purpose of this study is to accumulate the fundamental data representing groundwater of plastic film houses by means of water quality and its multivariate statistical analysis. Groundwater samples were collected in every two years since 2000 to 2004 from total 211 sites. According to the result of water quality analysis, ground water quality was suitable for irrigation purpose averagely. Correlation analysis showed that EC was highest positively correlated with $Mg^{2+}$ to 0.810(p<0.01), 0.776(p<0.01) in April and July, respectively. $NO_3-N$ was highest positively correlated with T-N to 0.794(p<0.01) in October. This result shows that it can lead to a different result even in similar case sometimes. Four factors were extracted through factor analysis in April and July, but five factors were extracted in October. The proportions of cumulative variance by the factor were 64.9, 60.2, and 70.7 in April, July, and October, respectively. The first factor was highly related to anions and cations such as $Ca^{2+},\;Mg^{2+},\;Cl^-,\;{SO_4}^{2-}$, and EC in contrast to that of stream water. According to the cluster analysis, 211 sites are classified into four groups. Common type of ground water quality was shown in group A. The pH and $PO_4-P$ were highest in Group B. The anions and cations were highest in Group C. $COD_{Cr}$ was highest in Group D.

Quality Characteristics of Fermented Pork with Korean Traditional Seasonings (한국 전통 양념을 이용한 발효 돼지고기의 품질 특성)

  • Jin, S.K.;Kim, C.W.;Lee, S.W.;Song, Y.M.;Kim, I.S.;Park, S.K.;Hah, K.H.;Bae, D.S.
    • Journal of Animal Science and Technology
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    • 제46권2호
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    • pp.217-226
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    • 2004
  • This study was canied out to evaluate the quality characteristics of the fermented pork with Korean traditional seasonings. The samples, outside muscle of pork ham were cut to cube(7 ${\times}$ 12 ${\times}$ 2cm) and five Korean traditional seasonings such as garlic paste(TI), pickled Kimchi(T2), pickled Kimchi juice(T3), soybean paste(T4), red pepper paste(T5) were seasoned by the proportions of meat to seasonings(1 : 1). The seasoned samples were fennented at - 1 ${\pm}$ 1$^{\circ}C$ for 20 days. According to proximate composition analysis, all pork samples contained protein 20 ${\sim}$ 22%, fat 3 ${\sim}$ 5%, moisture 64 ${\sim}$ 70% and ash 1.8 ${\sim}$ 2.0%. However, T5 had high crude fat level and relatively low moisture content. The highest pH among treatments was shown in TI whereas T3 showed the lowest. Water holding capacity(WHC) of T4 and T5 were higher, while those values were lower in T3 compared with other treatment. Shear force value was the highest in T5, while it was the lowest in T4. TBARS value of T3 was the highest, while that was the lowest in T4. Moreover the highest VBN value was observed in T4 due to fermentation of soy protein. However, the lowest VBN value shown in Tl indicated the inhibition of protein degradation by the garlic. The highest saccarinity was shown in T5 but it was the lowest of in T3. Salinity was shown to be high in T2 and low in T5. $L^*$ values of T4 was higher both at the surface and inner side of samples than the others but T5 showed the lowest value. T2 showed the highest $a^*$ value but T4 and T5 showed the lowest. In the result of sensory evaluation for cooked meat, T5 had the highest score in all item including overall acceptability, while T4 had the lowest score. Unsaturated fatty acid(UFA) ratio of T5 and n were 72.16 and 69.93 respectively, and the ratio of UFA/Saturated fatty acid(SFA) were higher in the order of T5 >T4> T3 >Tl >T2. Overall quality characteristics were higher in the order of T5 >T2 >Tl >T4 >T3.

Effect of Dietary Glycine Betaine on Pork Qualities During Storage (Glycine betaine의 급여가 돈육의 품질에 미치는 영향)

  • Park, G.B.;Hur, S.J.;Yang, H.S.;Lee, J.I.;Kwck, S.J.;Lee, J.D.;Huh, N.E.;Kim, J.S.;Joo, S.T.
    • Journal of Animal Science and Technology
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    • 제46권5호
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    • pp.821-832
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    • 2004
  • A total of 80 pigs were used to investigate the effect of dietary glycine betaine(N,N,N-trimethylglycine) on pork quality during cold storage. About 70 kg pigs were randomly a1loted into one of four experimental diet groups(0%, 0.2%, 0.4% and 0.6% glycine betaine). Pigs were slaughtered at approximately 110 kg live weight, and pH, color(CIE $L^* a^* b^*$), shear force, sarcomere length, lipid oxidation and composition of fatty acid were measured in pork loin for 13 days of cold storage. The concentration of glycine betaine in pork loin was significantly increased(P<0.05) with increasing of glycine betaine level in diet. Pork loins from dietary betaine groups showed significantly higher muscle pH and lower CIE $b^*$ values compared to control group after 13 days of storage. There were significant differences in shear force values among pork loins from diet groups at 24 hrs postmortem. However, pork loins from control diet showed longer sarcomere length than those of dietary betaine groups. Dietary glycine betaine increased the ratio of saturated fatty acids and decreased unsaturated fatty acids in pork loins. Especially the ratios of linoleic and myristic acid were decreased with increasing dietary betaine level. However, dietary glycine betaine did not affect lipid oxidation (TSARS) and sensory evaluation during cold storage.

Comparison between REML and Bayesian via Gibbs Sampling Algorithm with a Mixed Animal Model to Estimate Genetic Parameters for Carcass Traits in Hanwoo(Korean Native Cattle) (한우의 도체형질 유전모수 추정을 위한 REML과 Bayesian via Gibbs Sampling 방법의 비교 연구)

  • Roh, S.H.;Kim, B.W.;Kim, H.S.;Min, H.S.;Yoon, H.B.;Lee, D.H.;Jeon, J.T.;Lee, J.G.
    • Journal of Animal Science and Technology
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    • 제46권5호
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    • pp.719-728
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    • 2004
  • The aims of this study were to estimate genetic parameters for carcass traits on Hanwoo(Korean Native Cattle) and to compare two different statistical algorithms for estimating genetic parameters. Data obtained from 1526 steers at Hanwoo Improvement Center and Hanwoo Improvement Complex Area from 1996 to 2001 were used for the analyses. The carcass traits considered in these studies were carcass weight, dressing percent, eye muscle area, backfat thickness, and marbling score. Estimated genetic parameters using EM-REML algorithm were compared to those by Bayesian inference via Gibbs Sampling to find out statistical properties. The estimated heritabilities of carcass traits by REML method were 0.28, 0.25, 0.35, 0.39 and 0.51, respectively and those by Gibbs Sampling method were 0.29, 0.25, 0.40, 0.42 and 0.54, respectively. This estimates were not significantly different, even though the estimated heritabilities by Gibbs Sampling method were higher than ones by REML method. Since the estimated statistics by REML method and Gibbs Sampling method were not significantly different in this study, it is inferred that both mothods could be efficiently applied for the analysis of carcass traits of cattle. However, further studies are demanded to define an optimal statistical method for handling large scale performance data.

Determination of Energy Requirements for Maintenance in Hanwoo Steers (거세한우의 유지에너지 요구량 결정)

  • Kim, K.H.;Oh, Y.G.;Kim, W.;Lee, S.C.;Shin, K.J.;Jeon, B.T.
    • Journal of Animal Science and Technology
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    • 제46권2호
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    • pp.193-200
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    • 2004
  • This experiment was carried out to determine energy requirements for maintenance of Hanwoo steers. Nine Hanwoo steers weighing 376.6$\pm$12.5kg were used in this experiment and fed rice straw(44%) and concentrate (56%) at three different energy levels; 0.8 times maintenance(0.8M), 1.2 times(1.2M) and 1.6 times(1.6M), respectively. Dry matter intake was 48.5, 65.9 and 86.5g/$BW^{0.75}$ for 0.8M, 1.2M and 1.6M, respectively. Increase in energy intake with the increased DM intake did not affect digestibilities of dry matter, organic matter, crude protein, crude fiber, crude fat and nitrogen-free extract. Gross energy intake averaged 190.8, 255.8 and 340.9kcal/BW0.75 for 0.8M, 1.2M and 1.6M, respectively. Energy loss was 41% feces and 0.6${\sim}$1.5% urine of gross energy intake. Further, energy loss from methane produced during rumen fermentation was 5${\sim}$9%, while body heat loss averaged 40${\sim}$60%. Intercept of the regression equation between ME intake and retained energy indicated that energy requirement for maintenance was 124.3kcal $ME/BW^{0.75}$.

Physico-chemical Changes of Pork Prepared by Korean Traditional Sauces During Chilled Aging (한국 전통 양념으로 제조한 돼지고기의 숙성중 물리화학적 변화)

  • Jin, S.K.;Kim, I.S.;Hur, S.J.;Lyou, H.J.;Hah, K.H.;Joo, S.T.;Lee, J.I.
    • Journal of Animal Science and Technology
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    • 제46권5호
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    • pp.859-870
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    • 2004
  • This study was conducted to investigate physico-chemical changes of pork prepared by Korean traditional 3 types seasoning such as soybean sauce(Tl); garlic sauce(T2); red pepper sauce(T3). The seasoned samples were aged at 0 ${\pm}$ $1^{\circ}C$ for 30 days. The results obtained were as follows; Salinity(%) and saccharinity(%) of sauce were significantly decreased(P<0.05) among the all treatments during aging periods, whereas fermented meats were significantly increased(P<0.05). pH of sauce and fermented meat were tended to increased as aging period. Water-Holding Capacity(WHC) and shear force of fermented meat increased(P<0.05) among the all treatments at 20 days of storage, and decreased after that period. In color, $L^* and b^* value of T3 were lower, while a^*$ value was higher than other treatments during aging period. Volatile Basic Nitrogen(YBN) value of all treatments were increased(P<0.05) after 20 days of storage. YBN value of T3 was significantly higher(P<0.05) compared with other treatments. Thiobarbituric Acid Reactive Substances(TBARS) was rapidly increased until 10 days of storage and somewhat decreased up to 20 days after 10 days in all treatments, it was re-increased after 20th days. T3 showed the highest TBARS among the all treatment groups. Total bacterial and Lactobacilli spp. were significantly increased(P<0.05) in all treatments at 10 days of storage and decreased after that period, however it tended to increased at 30 days of storage. In sensory evaluation, overall acceptability in all treatments were increased after 20 days of storage compared to initial storage and it score was similar thereafter.