• Journal of Aquaculture
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• v.5 no.1
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• pp.19-27
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• 1992

A feeding study was conducted for 85 days to determine if soybean meal can be substituted for fish meal in a diet of coho salmon (Oncorhynchus kisutch) fingerlings. Solvent extracted soybean meal was used in the experimental diet at $0{\%},\;10{\%},\;30{\%},\;50{\%}$ and $70{\%}$ levels to make up about $40{\%}$ protein in the final diets. The results of feeding trial indicated that soybean meal can be used up to $30{\%}$ (replacing $34.3{\%}$ fish meal) as a major protein source with no adverse effects on the growth of coho salmon(P>0.05) . However, both growth rate and survival were decreased in the groups of fish fed over $50{\%}$ soybean meal diets.

• Journal of Aquaculture
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• v.10 no.3
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• pp.347-356
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• 1997

Feeding experiment was conducted to evaluate effects of dietary soybean meal (SBM) on the phosphorus bioavailability of rainbow trout (Oncorhynchus mykiss). Seven diets, containing 0~70% SBM, were made based on iso-nitrogenous and iso-caloric basis of 44% crude protein. Ajpparent digestibility of phosphorus was measured 60 and 120 days after feeding. The digestibility coefficient increased gradually as the level of SBM increased up to 46~58%, but decreased at 58~70% SBM level. Phosphorus concentrations in feces decreased with an increased in dietary SBM level. Patterns of post-prandial phosphorus excretion were not similar to those of ammonia excretion, but the amount decreased with the increased SBM level in the diets. Total excretion of phosphorus for 24 hours was 19.68mg.kg-1 body wt..day-1 in the control group, but excreted phosphorus by the 58% and 70% SBM groups was 63.4% and 56.1% of the control group, respectively. Phosphorus concentration of whole body decreased with an increase in dietary SBM level for 120 days of feeding. However, there were no significant differences in phosphorus concentrations of liver among the experimental groups, except for 70% SBM diet group.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.759-766
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• 2003

Two experiments were conducted to evaluate the nutritive values of defatted rice bran (DRB) and examine the effects of its dietary supplementation on broiler performances. In Experiment 1, to measure true metabolizable energy (TME), nitrogen corrected true metabolizable energy (TMEn), and true amino acid availability (TAAA), 30g of DRB sample was forced-fed to each of 16 ISA-Brown roosters followed by a 48h collection of excreta. The TME and TMEn values for DRB were 2.19 kcal/g and 2.05 kcal/g, respectively. The mean value of TAAA of the 15 amino acids in DRB was 77.29%. In Experiment 2, a total of 72 Avian broiler chicks were divided into 4 groups with 3 replicates of 6 birds per replicate, and fed one of the experimental diets containing 0, 5, 10 or 15% of DRB. Feed intake, weight gain, feed conversion rate, and body composition were measured for 3 weeks. Although there were no significant differences (P$\geq$0.05), body weight gain and feed intake of chickens fed a diet containing 15% DRB were slightly higher than those of the other groups. From these results, it can be concluded that feed formulation using TMEn and TAAA of DRB is an effective method for assuring feed quality and DRB can be supplemented to broiler rations up to 15% level.

• Applied Biological Chemistry
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• v.13 no.2
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• pp.153-170
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• 1970

1. Isolation and identification of amylase-producing bacteria. The powerful strain A-12 and S-8 were respectively isolated from air and soil after screening a large number of amylase-producing bacteria. Their bacterial characteristics have been investigated and it has been found that all characteristics of strain A-12 and S-8 are similar to Bac. subtilis of Bergey's manual except for the acid formation from a few carbohydrates and the citrate utilization, i.e., the strain A-12 shows negative in the citrate utilization, and the acid formation from arabinose and xylose, S-8 shows negative in the acid formation from xylose. 2. Amylase production by Liquid cultures with solid materials. Several conditions for amylase production by strain A-12 in stationary cultures have been studied. The results obtained are as follows. (1) The optimum conditions are:temperature $35^{\circ}C$, initial pH 6.5 to 7.0 and incubation time 3 to 4 days. (2) The amylase production is not affected by the preservation period of the stock cultures. (3) Among the various solid material, the defatted soy bean is found to be the best for t1e amylase production. However, the alkali treatment of the defatted soy bean gives no effect contrary to the cage of defatted rape seed. The addition of soluble starch to the alkali extract of defatted soy bean shows the increased amylase production. (4) Up to 1% addition of ethanol to carbon dificient media gives the improved amylase production, whereas the above effect is not found in the case of carbon rich media. (5) The amylase production can be increased 2.5 times when 10% of defatted soy bean is admixed to cheaply available wheat bran. (6) The excellent effect is found for amylase production when 20% of wheat bran is admixed to defatted dry milk which is a poor medium. The activity is found to be $D^{40^{\circ}}_{30'}$ 7,000(L.S.V. 1,800) in 10% medium. (7) No significant effect is observed due to the addition of various inorganic salts. 3. Amylase production by solid cultures. Several conditions for amylase production by strain A-12 in wheat bran cultures have been studied and the results obtained are as follows. (1) The optimum conditions: are temperature $33^{\circ}C$, incubation lime 2 days, water content added 150 to 175% and the thickness of the medium 1.5cm, The activity is found to be $D^{40^{\circ}}_{30'}$ 36,000(L.S.V. 15,000) (2) No significant effect is found in the case of the additions of various organic and inorganic substances.

• Journal of Aquaculture
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• v.9 no.3
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• pp.265-278
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• 1996

A long-term (160 days) feeding experiment was conducted to evaluate effects of dietary soybean meal (SBM) on growth of rainbow trout (Oncorhynchus mykiss). Fish fed diets containing up to $46\%$ of SBM showed the same growth rates with control during the first 60 days (p>0.05). However, growth showed a gradual decrease with the culture period. At the end of 160-days experiment, fish fed diets with above $34\%$ of SBM showed a decrease in growth rate compared to the control group. Fatness of experimental fish was reduced with an increase in dietary SBM levels and in feeding period. Hepatosomatic index was significantly lower from fish fed the diets containing above $46\%$ of SBM than that from fish fed the control diet throughout the 160 days of experimental period, and fish fed diet containing above $46\%$ of SBM appeared to make restricted utilization of carbohydrate sources. These results may suggest that soybean meal could be used up to $22\%$ in trout diet as a substitute for fish meal without unfavourable side effects.

• Journal of Aquaculture
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• v.9 no.4
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• pp.395-407
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• 1996

A feeding experiment for 160 days was conducted to evaluate effects of dietary soybean meal (SBM) on the apparent digestibility, ammonia excretion and body composition of rainbow trout (Oncorhynchus mykiss). Seven diets containing $0\~70\%$ SBM were formulated based on iso-nitrogenous and iso-caloric basis of $44\%$ crude protein and approximately 4,200 Kcal/kg of gross energy. To evaluate the nutritional utilization of the SBM diets at the end of 60 and 120-day rearing with test diets, digestion rates of protein and lipid of SBM diets were measured. Fish fed the diets containing above $46\%$ SBM showed higher apparent digestibility for protein, but lower for lipid than did fish fed the control diet in both trials. Protein and fat contents in the carcass were similar for all experimental fish, except for fish fed $58\%$ and $70\%$ SBM which showed lower fat content than the others, but composition of fatty acid and amino acids were not affected by dietary SBM levels. Gill and urinary post-prandial ammonia ($NH_3-N$) excretions were measured at 12 and 24 hours after single feeding of the experimental diets. Excretions of $NH_3-N$ measured after 24 hours were almost 1 times higher than those measured after 12 hours. Total excretion fer the 24 hours by fish fed $70\%$ SBM was $15\%$ higher than that of fish fed the control diet. Results of present study may suggest that the rainbow trout which were fed above $34%\;or\;46\%$ of SBM diet showed a decrease gradually in lipid bioavailability compared to the control group.

• Korean Journal of Rural Living Science
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• v.2
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• pp.1-23
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• 1991

농촌주민들의 영양 섭취실태에 대한 동향을 요약해 보면 1. 총 섭취식품중 약 90% 정도를 식물성식품으로써 섭취하고 있으며, 곡류가 차지하는 비율은 점차 감소되는 반면 콩류, 채소류 및 과일류의 섭취 비율은 현저히 증가하고 있다. 2. 총 열량섭취는 감소하는 추세이고 총열량에 대한 탄수화물 :단백질 :지방의 비율이 74 : 12 : 12의 수준이다. 총 단백질 섭취에 대한 동물성 단백질 섭취율은 36%로써 질적으로 바람직하게 변화되고 있다. 칼슘의 섭취수준이 권장량의 90%정도로서 조금 부족한 듯 하지만 철분, 비타민 등 대체로 영양섭취상태는 많이 개선되었음을 알 수 있다. 3. 농촌 영아들의 경우는 모든 영양소가 권장량에 현저히 미달되는 상태이다. 이유식품 개발 및 이유 방법에 대한 상담 지도가 적극 필요한 실정이다. 4. 새마을 유아원의 설치 및 유아급식의 혜택으로 농촌 유아들의 영양 섭취 상태는 두드러지게 개선되었으나 철분섭취 상태는 권장량에 미달되는 실정이다. 취학전 아동의 영양향상을 위해서는 유아 교육시설 확충 및 간식의 중요성에 대한 농촌 주부 대상의 영양 교육 확대 실시가 요청된다. 5. 국민학교 아동들에게서는 빈혈의 발생 빈도가 높게 나타났다. 성장 발육기에 있는 이들의 심신발달에 필요한 영양공급 및 올바른 식습관 형성을 위해서는 학교에서 일정한 지도 목표를 설정하여 계획적으로 실시하는 학교 급식의 필요성이 강조된다 하겠다. 6. 청소년기 및 임신 수유부의 경우도 철분의 섭취상태가 부족하기는 하지만, 철 결핍성 빈혈증상이 나타날 정도는 아니고 영양섭취상태도 대체로 양호하다. 7. 노인들의 식품 영양 섭취실태는 권장량에 크게 미달되는 실정이기는 하지만 혈액검사의 수치들이 대체로 정상인 범위에 있고 건강상태도 양호한 편이다. 식품 개발 및 조리법에 관해서 요약해 보면 1. 식량수급의 안정을 기하고자 다수확 품종인 통일계통 쌀의 취반 및 가공적성에 대한 검토가 진행되었으며, 잡곡류 및 감자류의 효과적인 이용을 위해 복합분의 사용에 의한 식품가공 적성에 대한 연구들이 실시되었다. 2. 콩류에 대한 연구로는, 기름을 추출하고 남은 탈지대두박의 단백질 식품으로의 이용을 위해 추출법의 검토 및 탈지대두박을 이용하는 두부제조법 등 다각적으로 식품에의 적용에 관해 연구가 진행 되었다. 3. 발효식품 중 김치에 대해서는 농촌지역에서 제조되는 김치의 종류와 섭취실태에 관한 연구 및 김치의 저장성을 증진시키기 위한 일련의 검토가 있다. 간장, 고추장, 된장에 대해서는 Koji를 이용한 개량식 제조법 및 팽화시킨 곡분을 사용하는 간편 제조법, 비용 절감을 위한 대체원료 이용에 대한 연구 등이 진행되었다. 4. 이밖에도, 병조림 제조방법, 어린이를 위한 고영양 보충식 개발, 생선의 폐기 부분을 이용하여 칼슘 급원 식품으로서의 유용성에 관한 조리과학적 검토 등이 실시되었다. 식습관 및 식생활 관리 지도에 관한 연구에 의하면, 농촌여성의 농업역할이 가중되면서 자녀들의 식생활을 방임하기 쉬운 실정이므로 유아 교육시설 및 학교 급식 등을 통해서 바람직한 식습관 형성을 위한 프로그램이 개발되어져야 하며, 한편, 식생활 담당자인 농촌주부에 대한 규칙적이고 정확한 영양교육이 이루어져야 하리라 본다.

• Applied Biological Chemistry
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• v.40 no.5
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• pp.404-408
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• 1997

Enzymatically hydrolyzed vegetable protein (eHVP) was produced from soy protein using proteases, and the physicochemical properties were examined. Soy protein hydrolysate of 6% protein and 50% degree of hydrolysis was useful for the base of savory ingredients. The Maillard-reacted and flavoring compound-added hydrolysate had improved flavor. It was for enzymatically hydrolyzed soy sauces and dehydrated seasonings. ISP hydrolysate of low molecular weight $(MW{\sim}250)$ and high protein content (85%) was suitable for special uses such as infant diets, sports nutrition, and medical diets. The eHVP gave no limitation of dosage in the formulation as a flavor enhancer. The byproduct of protein hydrolysis was found to have high content of fiber (21%) and to have potential for the use as dietary fiber or bulking agents.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.6
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• pp.758-762
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• 1993

The effect of sodium hexametaphosphate(SHMP) on the extraction of defatted sesame meal protein and the color and amino acid composition of protein concentrate have been studied. The highest amount of protein could be extracted with 1.5% SHMP and the extraction was effective at pH 12.0. The extraction rate tended to increase with increasing the flour to solvent ratio and about 60% of protein was obtained when adjusted the ratio to 1 : 40. Color of sesame protein concentrate was slightly improved by SHMP treatment. Lysine and methionine content were decreased in SHMP-treated protein concentrate but valine and leucine content were increased.

• Korean Journal of Food Science and Technology
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• v.9 no.3
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• pp.190-193
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• 1977

In our previous report (Korean J. Food Sci. and Technol., 9, 123, 1977) we have studied the functional properties of soy protein isolates prepared from defatted soybean meal. For the practical application of the data described, beef patties substituted with SPI, and imitatied ice cream were prepared and sensory evaluations were carried out. The sensory analyses indicated that the beef patties substituted with gelated SPI to 30% have no detectable difference to the control at the significant level of 0.01. The imitation of ice cream whose milk-solid-not-fat was substituted with soy protein isolates showed the same texture as the control. Flavor of ice cream, however, has significant difference to the control due to beany-off flavor. Therefore removal of beany-off flavor is required for the substitution in ice cream.