• Korean Journal of Microbiology
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• v.41 no.4
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• pp.275-280
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• 2005

Anthrax is an infectious disease caused by the gram-positive bacterium, Bacillus anthracis. Anthrax toxin is a tripartite toxin comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF). PA is the receptor-binding component, which facilitates the entry of LF or EF onto the cytosol. LF is a zinc-dependent metalloprotease, which is a critical virulence factor in cytotoxicity of infected animals. Therefore, it is of interest to develop its potent inhibitors for the neutralization of anthrax toxin. The first step to identify the inhibitors is the development of a rapid, sensitive, and simple assay method with a high-throughput ability. Much efforts have been concentrated on the preparation of powerful assays and on the screening of inhibitors using these system. In the present study, we have tried to construct anthrax lethal factor in yeast expression system to prepare cell-based high-throughput assay system. Here, we have shown the results covering the construction of a new vector system, subcloning of LF gene, and the expression of target gene. Our results are first trial to express LF gene in eukaryote and provide the basic steps in design of cell-based assay system.

• 건강소식
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• v.25 no.11 s.276
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• pp.24-25
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• 2001

• 월간산업보건
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• s.163
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• pp.19-22
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• 2001

• Agrochemical news magazine
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• v.21 no.7 s.160
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• pp.44-45
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• 2000

• Life and Agrochemicals
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• v.27 no.5 s.218
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• pp.44-45
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• 2006

• Agrochemical news magazine
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• v.25 no.7 s.200
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• pp.41-43
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• 2004

• Journal of the korean veterinary medical association
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• v.7 no.1
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• pp.16-19
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• 1963

• Journal of the korean veterinary medical association
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• v.30 no.4
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• pp.214-226
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• 1994

• Proceedings of the KAIS Fall Conference
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• 2011.05b
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• pp.924-925
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• 2011

수박 '스피드'에서 칼슘제를 착과 14일 후에 1회 엽면 살포한 후 식물체의 칼슘농도, 과실품질 및 탄저병 발생을 조사하였다. 식물체 부위별 칼슘농도는 엽병에서 ECa 500배 처리구는 다른 처리구보다 현저하게 높았으며 과피에서는 MCa 500배 및 염화칼슘 0.4%에서 현저하게 높았다. 과육에서는 무처리에 비하여 ECa, MCa 및 염화칼슘 모두 높았다. ECa 칼슘제 희석배수에 의한 엽병의 칼슘 농도는 500배, 250배, 125배에서 높았다. 수확 시 과중은 무처리 및 염화칼슘 0.4%에 비하여 ECa 및 MCa 처리에서 유의하게 증가하였다. 당도 및 과피두께 차이는 없었다. 탄저병 발생은 무처리에 비하여 처리 칼슘제 모두 낮았다. 생리장해과 발생은 육안으로 전처리 모두 관찰할 수 없었다.

• Research in Plant Disease
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• v.13 no.3
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• pp.164-169
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• 2007

Three kinds of disease occurring on ginseng leaves, such as grey mold, Alternaria blight, and anthracnose, started at the beginning of June, July, and August, respectively. The disease incidence was rapidly increased from the beginning of rainy season. To develop the control system with fungicides, 6 fungicides were selected and applied on ginseng at the indicated time. Calculating the control value by using the area under the disease progressing curve (AUDPC), the control activities of the supervised control system with fungicides were 61.7, 78.8 and 70.5% against grey mold, Alternaria blight, and anthracnose, respectively. The application of the mixture of carbendazim and diethofencarb on first of June was very important in control system with fungicides. If it was deleted in control system, control value was decreased against grey mold. In the case of Alternaria blight and anthracnose, the application of difenoconazole on July 18, and trifloxystrobin on August 7 were indispensible. If difenoconazole and trifloxystrobin were not applied on July 18, and August 7, the control activities against Alternaria blight and anthracnose, respectively, were decreased to 28.9% and 44.4%.