• Asian Journal of Turfgrass Science
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• v.17 no.2_3
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• pp.81-85
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• 2003

The plant parasitic nematodes were surveyed from Zoysia matrella, Agrotis palustris, and Poa pratensis at Anyang Benest Golf Club, Dongrae Benest Golf Club, and Gapyeong Benest Golf Club and from A. palustris at Castle Pine Golf Club. Criconema sp., Ditylenchus sp., Helicotylenchus sp., Meloidogyine sp., Pratylenchus sp., Tylenchus sp., and Tylenchorhynechus sp. were separated from unhealthy turf areas. In addition, Tylenchorhynechus dubius was firstly recorded from A. palustris at the green of Castle Pine Golf Club. Meloidogyne sp., Helicotylenchus sp., Ditylenchus sp., and Pratylenohus were separated from Z. matrella, Meloidogyne sp. and Pratylenchus were from A. palustris, and Meloidogyne sp., Tylenchus sp., Tylenchorhynchus sp., Criconema sp., and Helicotylenchus sp. were from P. pratensis. Out of separated nematodes, Meloidogyne was the most serious nematode and widely distributed.

• Weed & Turfgrass Science
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• v.3 no.1
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• pp.41-49
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• 2014

Four preparations of liquid turfgrass fertilizers were tested cool season turfgrasses during fall season. The preparations of PS-A, PS-B, PH-C and PH-D for commercial products were contained with essential nutrient elements, seaweed extract, amino acids, and humus substance. Growth and quality of creeping bentgrass (Agrostis palustris Huds cv. Penn-A1) and Kentucky bluegrass (Poa pratensis L.) mixture (Midnight 33%, Moonlight 33%, Prosperity 33%) were evaluated by normalized difference vegetation index (NDVI), root length and fresh weight, shoot density, turf color, and chlorophyll content. Three foliar sprays of 4 preparations with an interval of 7 days were made in the fall of 2013 in the sod production field at Hapchun, Korea. No significant difference among 3 preparations was found in NDVI of creeping bentgrass. However, PS-A was significantly increased NDVI of Kentucky bluegrass. Two to three applications of PS-A significantly increased chlorophyll content and turf color. Three foliar sprays of PS-A and PS-B were significantly increased the shoot density of Kentucky bluegrass on 20 days after final application. These results may indicate that the use of some preparation is beneficial in producing higher quality turfgrass sod with better color and chlorophyll content during fall season.

• Asian Journal of Turfgrass Science
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• v.12 no.2
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• pp.113-126
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• 1998

Wear simulator로 답압이 가해진 크리핑 벤트그라스 (Agrostis palustris Huds.) 훼어웨이에서 관수방법·예지물 순환 및 질소시비수준의 여러 가지 잔디관리 요인이 엽조직 및 토양질소 함유량에 미치는 효과를 알아보기 위해 본 연구를 수행하였다. 연구포장은 'Penncross'크리핑 벤트그라스 반디밭으로 1988년에 sharpsburg silty-clay loam (Typic Argiudoll) 토양에 조성하였으며, 실험은 1989년부터 1991년까지 3년간 수행하였다. Split-split-plot 실험디자인을 사용하여 주구에 daily or biweekly irrigation, 세구에 clipping return or removal, 세세구에 low-N (5g), moderate-N (15g), high-N (25g N m-2 yr-1)처리를 난괴법 3 반복으로 배치하였다. 생육기간중 잔디예초는 12mm 예고로 일주일에 4번 실시하였고, 기타 잔디관리는 high maintenance 수준으로 유지되는 한지형 양잔디 훼어웨이 기준으로 실시하였다. 엽조직 및 토양샘플은 1989년 2회, 1990년과 1991년에는 3회씩 채취하여, 네브라스카 주립대 토양식물분석실에서 분석하였다. 답압이 가해진 크리핑 벤트그라스 훼어웨이 잔디에서 엽조직 질소함유량은 여러 가지 잔디관리 방법간 상호작용 효과가 관찰되었다. 1989년 나타난 질소시비수준과 관수방법간의 상호작용에서 daily irrigation 지역의 엽질소 함유량은 질소시비량이 low-N 수준에서 high-N 수준으로 증가함에 따라 3.51%에서 3.94%로 quadratic pattern으로 증가하였다. High-N 처리지역에서 엽질소 함유량은 daily irrigation 관수방법이 biweekly irrigation 관수방법보다 약 4% 정도 더 많은 것으로 나타났다. 엽질소 함유량은 특히 질소시비 수준에 따라 크게 영향을 받는 것으로 나타났다. 1990년 질소시비량이 low-N 수준에서 high-N 수준으로 증가함에 따라 3.50%에서 4.25%로 quadratic pattern으로 증가하였고, 1991년에는 4.20%에서 4.60%까지 linear pattern으로 증가하였다. High-N 처리구의 엽조직 질소함유량은 low-N 처리구와 비교시 1990년에는 21%, 1991년에는 10% 더 많은 것으로 나타났다. 잔디조성후 시간이 경과함에 따라 엽조직의 질소함유량도 증가하였다. Low-N 수준에서 1991년 엽질소 함유량은 1990년에 비해 20% 증가하였으며, high-N 수준에서는 1991년의 엽조직 질소함유량이 1990년 보다 8% 증가한 것으로 나타났다. 따라서, 잔디조성후 경과기간에 따라 연간 시비량을 조절할 필요가 있으며, 특히 새로 조성된 잔디밭과 조성된 지 어느 정도 지난 기존 잔디밭간에 차별화된 관리프로그램이 필요한 것으로 판단되었다. 잔디관리에서 답압이 가중되는 정도에 따라 지역별로 장기간 차별화된 관리 접근을 해야하고, 정기적으로 토양 및 엽분석을 실시해서 시비프로그램에 활용하는 것이 필요하다 하겠다. 본 연구결과 나타난 잔디관리 요인간 상호작용효과는 잔디관리시 여러 가지 관리방법에 따른 효과를 입체적으로 분석해서 해당 골프장 현실에 적합한 통합적인 잔디관리(integrated turfgrass management)의 필요성을 제시한다고 할 수 있겠으며, 또한 답압가중 정도에 따른 잔디관리요인간의 반응효과차이는 향후 무답압 지역에서 실시된 연구결과를 답압을 받고 있는 경기장 및 골프장 등의 잔디밭에 적용할 경우에는 주의깊게 데이터 활용을 해야 되리라고 사료되었다.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.177-183
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• 2011

The susceptibility of cultivars of three bentgrass species (creeping, colonial, and velvet bentgrass) was evaluated on detached leaves assays with pink snow mold 9 isolates caused by Microdochum nivale in Petri dishes and whole plants under controlled conditions. The pink snow mold isolates obtained from infected turfgrasses on golf courses in Wisconsin were tested on response of fungicides and temperature. Detached leaf assay and susceptibility of bentgrass cultivars were evaluated with potted adult seeding during 80 days. Nine isolates were susceptible to two fungicides and were significantly different among isolates. Mycelial growth was varied in response of temperatures among isolates. There were significant differences in development and colonization of the fungus on detached leaf assay among bentgrass species include culvitars. There were significant differences on whole plants in disease severities among the three bentgrass species, particularly between tetraploids (creeping and colonial) and diploid (velvet) species, and among cultivars within each species, indicating that there are varying levels of susceptibility in species and cultivars to M. nivale. This study could be applied to evaluate the susceptibility of bentgrass to pink snow mold and also to predict a prospective evaluation of bentgrass cultivars to pink snow mold in fields in a breeding program.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.58-64
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• 2015

The black cutworm, Agrotis ipsilon (Hufnagel) is a major insect pest of economic crops including turfgrasses on golf courses. The entomopathogenic nematode, Steinernema carpocapsae GSN1 strain (ScG), a Korean isolate, is an effective biological control agent for soil dwelling and greenhouse insect pests in Korea. In addition, ScG is commercially produced in Korea. We conducted laboratory, greenhouse, and field trials to evaluate efficacy of ScG against black cutworms in turfgrasses. A rate of 63 infective juveniles (Ijs) per larva killed >90% of $3^{rd}$ instars feeding in cups of artificial diet within 3 days. In greenhouse trials against cutworms feeding in pots of turfgrass, efficacy of ScG was higher against $4^{th}$ instars than against $2^{nd}$ instars (90.0 vs 81.2% mortality, respectively, at $2,000Ijs\;pot^{-1}$) in perennial ryegrass, and higher against $3^{rd}$ instars in creeping bentgrass, Agrostis palustris than in zoysiagrass, Zoysia japonica (96.7 vs 52.5% mortality at $100,000Ijs\;m^{-2}$) in pot. The corrected mortality of $4^{th}$ instar was 79.9% at the rate of $100,000Ijs\;m^{-2}$ in the creeping bentgrass in the field. So ScG could be used as biological control agent against black cutworm in turfgrass of golf courses.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.54-59
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• 2012

This study was conducted to evaluate to the effect of liquid fertilizer with saponin (SLF) and liquid fertilizer with amino acid (ALF)on the growth of creeping bentgrass. In creeping bentgrass, turf color index, chlorophyll index, dry weight and shoot number were measured. It was hardly affected by SLF and ALF applications in investigation of chemical properties of the soil. By applying SLF and ALF, turf color index and chlorophyll index in 2SLF and 2ALF were increased more than CF, and shoot number and root length in 2SLF, ALF and 2ALF were higher than CF. In correlation coefficient among growth factors of creeping bentgrass, turf quality was significantly different in root length, shoot number, dry weight, and content of N and K in turf tissue (P<0.05), N content of tissue was significantly in root length, shoot number and dry weight (P<0.05), and K content was significantly in shoot number and dry weight (P<0.05). These results suggested that application of functional liquid fertilizers such as SLF and ALF was expected to replace compound fertilizer in turf management and that applied SLF and ALF was stimulated the uptake of N and K into turf so that turf qualities were improved by enhancing growth shoot and root of turf.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.24-30
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• 2010

We investigated occurrence of yellow patch for early spring season of 48 cultivars in 5 species of turfgrass, most popularly used in Korea golf courses. Only yellow patch disease caused by Rhizoctonia cerealis was occurred naturally from late fall season. Perennial ryegrass and creeping bentgrass cultivars showed susceptibility from yellow patch in early spring, respectively. However, among There was no significantly different between the other species. Creeping bentgrass, 'Pennlinks II' cultivar was the most susceptible. Yellow patch symptom showed reddish brown with margins in color and patch showed size from 5 cm to 50 cm in diameter. Based on our results, Yellow patch showed large differences among cultivar and each turfgrass species.

• Horticultural Science & Technology
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• v.35 no.3
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• pp.344-360
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• 2017

Zoysiagrasses are important turf plants used for school playgrounds, parks, golf courses, and sports fields. The two most popular zoysiagrass species are Zoysia japonica and Zoysia sinica. These are widely distributed across different growing zones and are morphologically distinguishable from each other; however, it is phenotypically difficult to differentiate those that grow along the coastal line from those in beach area habitats. A combination of morphological and molecular approaches is desirable to efficiently identify these two plant cultivars. In this study, we used a rapid identification system based on DNA barcoding of the nrDNA-internal transcribed spacer (ITS) regions. The nrDNA-ITS regions of ITS1, 5.8S nrDNA, and ITS2 from Z. japonica, Z. sinica, Agrostis stolonifera, and Poa pratensis were DNA barcoded to classify these grasses according to their molecular identities. The nrDNA-ITS sequences of these species were found at 686 bp, 687 bp, 683 bp, and 681 bp, respectively. The size of ITS1 ranged from 248 to 249 bp, while ITS2 ranged from 270 to 274 bp. The 5.8S coding region ranged from 163 - 164bp. Between Z. japonica and Z. sinica, nineteen (2.8%) nucleotide sites were variable, and the G+C content of the ITS region ranged from 55.4 to 63.3%. Substitutions and insert/deletion (indel) sites in the nrDNA-ITS sequence of Z. japonica and Z. sinica were converted to cleaved amplified polymorphic sequence (CAPS) markers, and applied to the Zoysia grasses sampled to verify the presence of these markers. Among the 62 control and collected grass samples, we classified three groups: 36 Z. japonica, 22 Z. sinica, and 4 Z. japonica/Z. sinica hybrids. Morphological classification revealed only two groups; Z. japonica and Z. sinica. Our results suggest that used of the nrDNA-ITS barcode region and CAPS markers can be used to distinguish between Z. japonica and Z. sinica at the species level.

• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.4
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• pp.259-264
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• 2001

To investigate the physiological responses to naturally occurring winter freezing stress in creeping bentgrass, changes in carbohydrates were monitored during winter period. Turf quality and leaf growth was nearly parallel with temperature fluctuation. The concentration of glucose, fructose and sucrose in both shoot and root gradually increased from November to January, and then sharply decreased until April. Sucrose was the largest pool of soluble sugars. Fructan also slightly accumulated in both shoot and roots from November to February. Fructan hydrolysis in both organs was found to be much active between February to April. Shoot contained largely higher carbohydrate content in all compounds examined than roots did. Fructan was found to be a main carbohydrate storage form, showing the highest concentration (176.7 and 126.7 mg g-' DW for shoot and root in February). The depolymerization of fructan from February coincided with the high declines in mono- and disaccharide. These results suggest that the accumulation of non-structural carbohydrate until January could be associated with freezing tolerance, and the active decrease from February with shoot regrowth.

• Asian Journal of Turfgrass Science
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• v.12 no.3
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• pp.173-182
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• 1998

This study was conducted to evaluate the effect of plant growth inhibitors on "Penncross" creeping bentgrass in the green of golf course for increasing the summer quality. Chlormequat chloride (C) 250$\times$, 500$\times$, 1000$\times$, daminozide (D) 50$\times$, 100$\times$, 200$\times$, paclobutralzol (P) 1000$\times$, 2000$\times$, 4000$\times$, trinexpac-ethyl (T) 1000$\times$, 2000$\times$, 4000$\times$, uniconazole (U) 25$\times$, 50$\times$, 100$\times$ with three concentrations, respectively, were applied to creeping bentgrass. The leaf length, leaf width, and internode length of stolon were inhibited by all plant growth retardants applied as compared to that of control in which D-50, T-2000, and trinexpac-ethyl were the most effective among treatments on the inhibition of leaf length and internode length of stolen, respectively. Leaf thickness increased more in the treatments of C-250, D-50. T-2000 and T-4000 than any other treatments. On the other hand, shoot density which was represented by number of leaves per unit area was found to be the highest in P-2000 and P-4000 treatment. In this case, all plant growth retardants with no significant differences showed increase of 15-20% in density as compared to control. Total dry weight increment was higher in P-2000 and P-4000 while leaf dry weight increment was higher in T-4000. and U-25 and 50 than other treatments. According to the visual evaluation for the purpose of turf quality measurement, it was found that paclo-butralzol, trinexpac-ethyl and uniconazole were not suitable for green maintenance and extension because of making leaves to yellow. In conclusion, it was postulated that C-250, D-50, and T-4000 had possibility to use practically in the green area of golf course.lf course.