• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.337-346
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• 2022

The purpose of this study was to investigate the antioxidant and anti-inflammatory activities of hot water (AMPW) and 70% ethanol (AMPE) extracts of apple mango (Mangifera indica L.) peel. The antioxidant activities were measured using a total polyphenol, electron-donating, 2,2'-azinobis [3-ethylbenzothiazoline6-sulfonic acid] (ABTS) radical scavenging assay. The total polyphenol content of AMPW and AMPE was 66.08 ± 0.62 mg TAE/100 g and 100.13 ± 0.23 mg TAE/100 g, respectively. As a result of measuring the electrondonating ability, at a concentration of 1,000 ㎍/ml, AMPW and AMPE showed an effectiveness of 86% and 94%, respectively. The ABTS assay showed 80% and 98% respective radical scavenging activity for AMPW and AMPE, at a concentration of 1,000 ㎍/ml. The cell viability on macrophage cells was performed using a 3-[4,5-dimethyl-thiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide (MTT) assay, and the results showed more than 90% cell viability at a 100 ㎍/ml concentration. Anti-inflammatory activity was verified by confirming nitric oxide (NO) production inhibitory activity, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) protein and mRNA expression inhibitory activity from lipopolysaccharide (LPS)-treated RAW 264.7 cells. The NO production inhibitory effects were measured using the Griess assay, which confirmed 45% and 40% inhibition after treatment with AMPW and AMPE, respectively. Moreover, the protein and mRNA expression of inflammatory-related factors iNOS and COX-2, decreased in a concentrationdependent manner. In conclusion, this study showed antioxidant and anti-inflammatory effects of Mangifera indica L. peel and revealed its promising potential for application as an antioxidant and anti-inflammatory agent.

• Pediatric Infection and Vaccine
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• v.30 no.2
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• pp.84-90
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• 2023

Purpose: Infants aged ≤90 days with fever are susceptible to severe infections. This study aimed to analyze the clinical features of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in this particular age group. Methods: Infants aged ≤90 days who were diagnosed with coronavirus disease 2019 (COVID-19) and hospitalized between March 1, 2020, and May 1, 2022 were included. Medical records of patients were retrospectively reviewed. Results: A total of 105 infants with COVID-19 were included; 27 (25.7%) neonates aged <28 days, and 48 (45.7%) and 30 (28.6%) infants aged 28-59 days and 60-90 days, respectively. Five (4.7%) patients remained asymptomatic and 68 (62.8%) were febrile, with a median fever duration of 2 days. The most common symptoms were respiratory including cough (66.6%), nasal stuffiness (51.4%), and rhinorrhea (40.9%). Blood cultures were performed in 10 infants but no organisms were detected. Cultures of bag-collected urine specimens from 8 infants were grown, resulting in positive growth for 2 without pyuria. Nine (8.6%) infants were treated with empirical antibiotics for a median duration of 2.3 days (range, 1-7 days). All 105 infants showed improvement without any complications, and there were no fatal cases. Conclusions: In this study, most infants aged ≤90 days with COVID-19 presented with mild symptoms and none of those evaluated had documented bacterial co-infection. The favorable prognosis among young infants with SARS-CoV-2 may aid clinicians in tailoring their approach to evaluation and management during outbreaks.

• Journal of Korean Tunnelling and Underground Space Association
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• v.11 no.4
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• pp.327-351
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• 2009

Laboratory tests are performed in this paper to investigate the brittle failure characteristics of over-stressed rocks taken in deep depth. Also, numerical simulation performed using that the so-called CWFS(Cohesion Weakening Frictional Strengthening) model is known to predict brittle failure phenomenon reasonably well. The most typical rock types of Korean peninsula - granite and gneiss - were used for testing. Results of uniaxial compression tests showed that the crack initiation stress was about 41 % to 42% of the uniaxial compressive strength regardless of rock types, where as, the crack damage stress of granite was about 75%, and that of gneiss was about 97%. Through the damage-controlled test, strength parameters of each rock were obtained as a function of damage degree. After the peak, the crack damage stress and the maximum stress were decreased, The cohesion was decreased and the friction angle was increased with increase of rock damage. Before reaching the peak, the elastic modulus was slightly increased, while decreased after the peak. Poisson's ratio was increased as the damage of rock proceeds. Comparison of uniaxial compression tests and damage-controlled tests shows the crack initiation stress estimated from the damage-controlled test fluctuated within the range of crack initiation stress obtained from the uniaxial compression test; the crack damage stress was less than that estimated from the uniaxial compression test. In order to predict the critical depth that brittle failure occurs, numerical simulations using the CWFS model were performed for an example site. Material parameters obtained from the laboratory tests mentioned above were used for CWFS simulation. Comparison between the critical depth predicted from the numerical simulation using the CWFS model and that predicted by using the damage index proposed by Martin et al.(l999), showed that critical depth cannot be reasonably predicted by the currently used damage index except for circular tunnels. A modified damage index was proposed by the author which takes the shape of tunnels other than circular into account.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.2
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• pp.17-24
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• 2023

A post-processing technique for the measurement signal of a solenoid-type sensor is introduced. The solenoid-type sensor nondestructively evaluates an external tendon of prestressed concrete using the total flux leakage (TFL) method. The TFL solenoid sensor consists of primary and secondary coils. AC electricity, with the shape of a sinusoidal function, is input in the primary coil. The signal proportional to the differential of the input is induced in the secondary coil. Because the amplitude of the induced signal is proportional to the cross-sectional area of the tendon, sectional loss of the tendon caused by ruptures or corrosion can be identified by the induced signal. Therefore, it is important to extract amplitude information from the measurement signal of the TFL sensor. Previously, the amplitude was extracted using local maxima, which is the simplest way to obtain amplitude information. However, because the sampling rate is dramatically decreased by amplitude extraction using the local maxima, the previous method places many restrictions on the direction of TFL sensor development, such as applying additional signal processing and/or artificial intelligence. Meanwhile, the proposed method uses amplitude demodulation to obtain the signal amplitude from the TFL sensor, and the sampling rate of the amplitude information is same to the raw TFL sensor data. The proposed method using amplitude demodulation provides ample freedom for development by eliminating restrictions on the first coil input frequency of the TFL sensor and the speed of applying the sensor to external tension. It also maintains a high measurement sampling rate, providing advantages for utilizing additional signal processing or artificial intelligence. The proposed method was validated through experiments, and the advantages were verified through comparison with the previous method. For example, in this study the amplitudes extracted by amplitude demodulation provided a sampling rate 100 times greater than those of the previous method. There may be differences depending on the given situation and specific equipment settings; however, in most cases, extracting amplitude information using amplitude demodulation yields more satisfactory results than previous methods.

• Journal of Korea Entertainment Industry Association
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• v.13 no.6
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• pp.247-256
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• 2019

The importance of the convergence education is getting more and more emphasized. But the university convergence education has not yet met today's needs. So this study is focused on showing effective practice methods and finding development directions of the university convergence criticism education. One of a significant trend of the contemporary academic education of liberal arts is the tendency of the convergent and integrative studies. What is essential in the convergent and integrative studies creativity. The zeitgeist of knowledge-based society is change and innovation. In response to its request, college education is being asked to do convergence education, And general education as the basis of convergence education is strengthened.

• Food Science and Preservation
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• v.31 no.2
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• pp.315-323
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• 2024

This study aims to confirm the anti-inflammatory activities of acemannan and aloesin, which have been studied for various efficacies at various mixed sample ratios. The mixed samples were mixed at a ratio of 1:1 (AA-1), 1:2 (AA-2), 1:3 (AA-3), 2:1 (AA-4), and 3:1 (AA-5). Seven samples were evaluated for their cytotoxic ability on macrophages, and the results showed that all cell viability was over 90% at a concentration of 100 ㎍/mL. First, due to the NO production inhibitory activity, a better inhibitory effect was achieved when using a mixed sample rather than a single material. Afterward, the activity of inhibiting the production of PGE₂, TNF-α, and IL-6 was confirmed using a mixed sample. It was confirmed that AA-2 had the best inhibitory activity on producing PGE2, TNF-α, and IL-6 rather than AA-1, AA-3, AA-4, and AA-5. For this reason, experiments were conducted using AA-2 to determine the protein expression levels of iNOS and COX-2, which are inflammation-related proteins. It was confirmed that AA-2 inhibited iNOS and COX-2 protein expression by 25.01% and 27.27%, respectively, compared to the LPS-alone treatment group. In conclusion, the mixed sample of acemannan and aloesin is judged to have anti-inflammatory activity and can potentially to be used as a functional material.

• Journal of the Korean Society of Radiology
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• v.82 no.5
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• pp.1258-1273
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• 2021

Purpose The balloon-stent technique (BST) has certain strengths as an assisted technique for the treatment of complex aneurysms. After Atlas release, the BST can be executed without an exchange maneuver of the balloon to the stent-delivery catheter. The purpose of this article is to share our experience with the BST using the Scepter-Atlas combination. Materials and Methods Device inspection led us to a simple method to avoid failure in loading Atlas to the Scepter. From March 2018 to December 2019, 57 unruptured distal internal carotid artery (dICA) aneurysms were treated with coil embolization; among which, 25 aneurysms in 23 patients were treated with BST. Clinical and angiographic data were retrospectively collected and reviewed. Results The technical success rate of the Scepter-Atlas combination increased from 50% to 100% after careful inspection. BST angiographic results were comparable to the stent-assisted coil (SAC) group treated during the immediately post-embolization same period (modified Raymond-Roy classification [MRRC] 1 & 2 84% in BST, 96.3% in SAC) and during short-term follow-up (MRRC 1 & 2 95.8% in BST, 88.4% in SAC). A small number of patients showed periprocedural complications, but none had clinical consequences. Conclusion BST using the Scepter-Atlas combination can provide an effective and safe method for the treatment of dICA aneurysms. Scepters can be used as delivery catheters for Atlas.

• Journal of Life Science
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• v.34 no.5
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• pp.339-355
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• 2024

The pulmonary system is a highly complex system that can only be understood by integrating its functional and structural aspects. Hence, in vivo animal models are generally used for pathological studies of pulmonary diseases and the evaluation of inhalation toxicity. However, to reduce the number of animals used in experimentation and with the consideration of animal welfare, alternative methods have been extensively developed. Notably, the Organization for Economic Co-operation and Development (OECD) and the United States Environmental Protection Agency (USEPA) have agreed to prohibit animal testing after 2030. Therefore, the latest advances in biotechnology are revolutionizing the approach to developing in vitro inhalation models. For example, lung organ-on-a-chip (OoC) and organoid models have been intensively studied alongside advancements in three-dimensional (3D) bioprinting and microfluidic systems. These modeling systems can more precisely imitate the complex biological environment compared to traditional in vivo animal experiments. This review paper addresses multiple aspects of the recent in vitro modeling systems of lung OoC and organoids. It includes discussions on the use of endothelial cells, epithelial cells, and fibroblasts composed of lung alveoli generated from pluripotent stem cells or cancer cells. Moreover, it covers lung air-liquid interface (ALI) systems, transwell membrane materials, and in silico models using artificial intelligence (AI) for the establishment and evaluation of in vitro pulmonary systems.

• Journal of Animal Science and Technology
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• v.45 no.6
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• pp.1067-1078
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• 2003

This study was designed to determine the effect of gestation housing and parity on the farrowing performance and behaviour of sows during pregnancy, farrowing and lactation periods. Total 18 Duroc sows were randomly assigned after 4 weeks of gestation to either an individual gestation stall or a groups of three with pen gestation system for three parities(1st, 2nd${\sim}$3rd, and 4th${\sim}$5th). Approximately 7 days before predicted farrowing date, sows were transferred to farrowing crates where they remained until 21 days post-partum. Behaviour was recorded on day 60 and 90 of pregnancy for 24 hours, on day of farrowing for farrowing duration and on day 10 of lactation for 2.5 hours in the farrowing crate. The results obtained from this study were summarized as follows: On 60 and 90 day of pregnancy, all sows in both groups made attempts for ventral lying more than for other postures. Sows in the group gestation pen spent more time on walking than those in individual gestation stall(p<0.01); however, the group housed sows were spent less time on drinking than the individually housed sows(p<0.01). There was no significant difference between gestation housings in the occurrence of stereotypy of pregnant sows. On day 60 of pregnancy, sows in the individual gestation stall showed the highest frequency of vacuum chewing and head weaving. However, floor licking and bar licking behaviours were highest in day 90 pregnant sows individually housed. The sows individually housed during the gestation period spent more time on sitting and eating on the day of farrowing in the farrowing crate than the sows group housed. On day 10 of lactation in the farrowing crate, the group housed sows during the gestation period made significantly more attempts for ventral lying than sows individually housed. In conclusion, the occurrence of stereotypy of sows during the gestation and lactation did not differ between gestation housings and farrowing performance of sows during the gestation period was not affected by gestation housing. Thus, the group housing for pregnant sows is a feasible means to improve welfare of sows and to increase the normal behaviour of sows.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.53-59
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• 2014

This study aimed to determine an appropriate cooling timing in the root zone for lowering substrate temperature and its effect on physiological response of sweet pepper (Capsicum annum L. 'Orange glory') grown on coir substrate in summer, from the July 16 to October 15, 2012. Daily temperature of substrate, root activity, leaf water potential, first flowering date, and the number of fruits were measured by circulating cool water through a XL pipe in the root zone during either all day (all-day) or only night time (5 p.m. to 3 a.m.; night) from the July 23 to September 23, 2012. For comparison, no cooling (control) was also applied. Between the $23^{rd}$ of July and $31^{st}$ of August (hot temperature period), daily average temperatures in substrates were $25.6^{\circ}C$, $26.1^{\circ}C$, and $29.1^{\circ}C$ for the all-day and night treatment, and control respectively. About 1.8 to $5^{\circ}C$ lower substrate temperature was observed in both treatments compared to that of control. In sunny day ($600-700 W{\cdot}m^{-2}{\cdot}s^{-1}$), the highest temperature of substrate was measured between 4 p.m. and 5 p.m. under both the all-day and night treatments, whereas it was measured between 7 p.m. and 8 p.m. under the control. Substrate temperatures during the day (6 a.m. to 8 p.m.) and night (8 p.m. to 6 a.m.) differed depending on the treatments. During the day and night, averaged substrate temperature was lower about $3.3^{\circ}C$ and $4.0^{\circ}C$ for the all-day, and $2.1^{\circ}C$ and $3.4^{\circ}C$ for the night treatment, compared to that of control. In the all-day and night treatment, the TD [TD = temperature of (control)] was greater in bottom than that of other regions of the substrate. Between the day and night, no different TD values were observed under the all-day treatment, whereas under the night treatment there was difference with the greatest degree in the bottom of the substrate. During the hot temperature period, total numbers of days when substrate temperature was over $25^{\circ}C$ were 40, 23 and 27 days for the control, all-day, and night treatment, respectively, and the effect of lowering substrate temperature was therefore 42.5% and 32.5% for the all-day and night treatment, respectively, compared to that for the control. Root activity and leaf water potential of plants grown under the all-day treatment were significantly higher than those under the night treatment. The first flowering date in the all-day treatment was similar to that in the night treatment, but 4-5 day faster than in the control. Also, the number of fruits in both treatments was significantly higher than that in the control. However, there was no effect of root zone cooling on eliminating delay in fruiting caused by excessively higher air temperature (> $30^{\circ}C$), although the substrate temperature was reduced $18^{\circ}C$ to $5^{\circ}C$. These results suggest that the method of cooling root zone temperature need to be incorporated into the lowering growing temperature for growth and fruit set of health paprika.