• Korean Journal of Microbiology
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• v.42 no.4
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• pp.294-298
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• 2006

An immunoaffinity chromatography for the specific binding of Streptomyces somaliensis phospholipase D (PLD) that is considered as an industrially potential enzyme was developed. By using the protein structure prediction programs and the X-ray crystal structure of a Streptomyces PLD, 5 different epitopes with high antigenicity that are predicted to locate on the surface of the S. somaliensis PLD were selected and then synthesized for the preparation of antipeptide antibodies. Each purified rabbit IgG was coupled with NHS-activated Sepharose to prepare the immunoaffinity resins. After one-step purification of the culture concentrate on the antipeptide IgG-coupled Sepharose column, SDS-PAGE and the Western blot analysis of the purified samples showed that purification of PLD on the affinity columns was satisfactory, indicating that the peptide design using the structural information of Streptomyces PLDs was rational. However, the purified PLD in the solution aggregated rapidly, which resulted in poor specific activity and low purification yield.

• Journal of Korean Society of Environmental Engineers
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• v.28 no.5
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• pp.472-479
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• 2006

In this research, speciation of phosphorus in sediment and overlying water dependent upon pH and ORP(Oxidation Reduction Potential) was studied. Three possible conditions were simulated: open system with circulation, closed system with stratification and closed system with sand capping on the sediment. Phosphorus release rate from sediment was increased for both open system and closed system if pH was less than 6.0. Phosphorus concentration for closed system was increased from 0.9 mg/L to 0.51 mg/L, and stabilized at 0.34 mg/L if anaerobic conditions were maintained in the overlying water. When sand capping was implemented, phosphorus concentrations of overlying water were maintained less than those of closed system.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.26 no.1B
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• pp.125-130
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• 2006

In this research, possibility of sand capping was experimentally evaluated to control phosphorus release from lake sediment into water body. Three acrylic columns without and with 40 and 80 mm of sand capping were prepared. Phosphorus concentrations of overlying water in these columns were measured. Performances of sand capping were evaluated for 0, 40, and 80 mm of capping thickness by measuring DO, ORP, TP, and $PO_4$-P. For the case without capping, the releasing rate of total phosphorus was higher and dissolved oxygen decreased faster, comparing with those of columns with capping. Total phosphorus concentrations in overlying water were inversely proportional to capping thickness, while phosphate concentration showed no significant differences between both cases. The experiment results suggested that sand capping is effective to retard total phosphorus release from sediment.

• Proceedings of the KAIS Fall Conference
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• 2002.05a
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• pp.134-136
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• 2002

이온교환 수지는 이온성 물질을 제거하는 가장 신31성 있는 단위공정일 뿐만 아니라 재사용의 측면에서 매우 경제적이다. 특히 토양화학 분야에서 지난 한세기 동한 수많은 연구가 진행되어 왔으며, 이온교환은 연수 및 탈염공정, 특정물질의 제거 및 회수, 토양을 통한 이온성 물질의 이동에서 널리 이용되고 있다. 이온의 선택도는 이온의 수화반경과 용액의 농도, 이온의 원자가에 따라 좌우된다. 본 연구는 양이온 평형실험과 칼럼실험을 통해 이온사이의 선택도 순서와 바탕음이온에 따른 특성을 조사하였다. 이 실험의 결과에서 양이온의 선택성의 농도가 낮을수록, 이온의 원자가가 높을수록 증가하였다. 평형실험의 양이온 선택도 순서는 H/sup +/< K/sup +/< Cu/sup 2+/ < Co/sup 2+/ < Ca/sup 2+/ < Ce/sup 3+/ 이며, 양이온 칼럼 순서도 동일함을 알 수 있다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.2628-2633
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• 2009

In a double deflector employed microcolumn, the variation of FOV and scan field width was investigated in mode of conversely biased double deflector to eliminate barrel distortion caused by aberration. The relationship between biased voltage of each deflector and electron emission tip voltage was studied for the maximum FOV and scan field width. The limitation and the linearity of zooming current image are also estimated as a function of electron emission tip voltage.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.4
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• pp.709-714
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• 2006

The propose of this study was to investigate the feasibility of using waste lime core to remove phosphorus from wastewater in continuous flow reaction. The phosphorus was found to be removed from municipal wastewater by hydroxyapatite crystallization and precipation. Waste lime core size 1, 2 showed phosphorus removal rate of about 90% during early 11 hrs of run time. In addition, breakpoint time was decreased by increased inflow rate regardless of waste lime core size.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.162-165
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• 2006

유해성 유기물질로 오염된 토양의 복원을 위한 토양세척공정에서 계면활성제를 선택적으로 재이용하기 위해 활성탄을 이용한 흡착 칼럼의 성능을 평가하였다. 계면활성제로는 Triton X-100을 소수성 유해 유기물질로는 다환방향족 탄화수소의 하나인 phenanthrene를 사용하여 그 성능을 평가하였다. 계면활성제의 흡착은 phenartthrene에 비해 빠른 흡착 특성을 보였으며 phenanthrene은 계면활성제의 포화흡착상태에서도 지속적으로 흡착이 이루어졌다. 이는 계면활성제의 흡착이 종료된 이후에도 유해성 유기물질의 지속적인 흡착을 기대할 수 있어 계면활성제의 지속적인 재이용과 활성탄 사용시간의 증대를 가져올 수 있음을 의미한다. 이와 같은 활성탄 칼럼에서의 유해성 유기물질의 우수한 선택적 흡착 결과는 기본적으로 소수성 정도의 차이와 size exclusion에 의한 기작뿐만 아니라 활성탄에 흡착된 계면활성제에 의한 다환방향족 탄화수소의 추가적인 흡착이 일어나 전체적인 성능이 향상되는 것에 기인한다.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.881-884
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• 2013

RDF(Resource Description Framework)는 시맨틱 웹의 기초로서 웹 사용자에게 정보를 보다 정확하고 효율적으로 접근하는 표준이다. RDF 데이터를 효율적으로 저장하고 접근하는 필요성이 날로 증가하고 있다. RDF 데이터를 저장하고 검색하는 기본 저장 구조는 관계형 데이터베이스를 이용하는 것이다. 최근에는 RDF 데이터가 엄청나게 증가하고 있는 시점에 대용량 database의 질의(단순 조회)에 최적화된 칼럼-지향(column-oriented) 데이터베이스가 대안으로 제안되었다. 본 논문에서는 RDF 데이터의 저장 구조로서 관계형 데이터베이스와 칼럼-기반 데이터베이스를 비교분석 하고자 한다. Berlin SPARQL Benchmark 를 이용한 성능분석 결과 RDF data 의 저장 구조로서 칼럼-기반 데이터베이스의 효율성을 입증하였다.

• KOREAN POULTRY JOURNAL
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• v.31 no.8 s.358
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• pp.128-129
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• 1999

• 월간 한농연
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• s.73
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• pp.2-2
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• 2008