• Restorative Dentistry and Endodontics
• /
• v.27 no.3
• /
• pp.232-238
• /
• 2002

연구목적 : Cytokine은 유해 미생물에 대한 숙주의 방어기전으로서의 염증반응에서 숙주세포 상호간의 작용을 매개해 주는 역할을 하며, 인간의 치수조직에서도 그 존재가 확인된 바 있다. Interleukin-6와 Interleukin-10은 염증의 초기에 작용하는 cytokine으로 알려져 있으나, 치수 및 치근단 질환에서의 역할과 상호작용에 대해서는 잘 알려져 있지 않다. 본 연구에서는 치수염의 원인균으로 알려진 Prevotella nigrescens를 이용하여 백서의 치수염을 유도한 후 시간의 변화에 따른 Interleukin-6, Interleukin-10의 농도의 변화를 측정하여 이들의 치수염에서의 작용을 알아보는 것을 목적으로 한다. 방법 : 실험적으로 치수의 염증반응을 일으키기 위하여 치수염의 원인균으로 알려진 Prevotella nigrescens를 이용하였다. 실험동물의 하악절치의 incisal tip부분을 절단한 후(n=120), 치수강을 개방시켰다. 실험군에서는 Prevotella nigrescens를 멸균된 면구에 묻혀서 개방된 치수강 내에 접종하였으며, 대조군에서는 균을 접종하지 않고 멸균된 면구만을 개방된 치수강 내에 위치시켰다. 그 후 1, 2, 5일이 경과되었을 때 실험에 사용된 치아를 발치하여, 치수조직을 적출하였다. Amersham사의 ELISA kit를 사용하여 적출된 치수조직내의 Interleukin-6와 Interleukin-10의 양을 측정하였으며 그 결과를 Mann-Whitney rank sum test를 사용하여 통계학적 유의성을 검증하였다. 조직학적 검사를 위해서는 발치된 치아를 nitric acid를 사용하여 탈회시킨 후 헤마톡실린-에오신 염색을 시행한 후 관찰하였다. 결과 : 1) Interleukin-6의 농도는 균접종 후 1일, 2일, 5일 모두에서 실험군에서 대조군보다 높게 나타났으며, 균접종 1일째의 결과는 통계적 유의성이 있었다(P<0.05). 2) Interleukin-10의 농도는 균접종 후 1일, 2일, 5일 모두에서 실험군에서 대조군보다 높게 나타났으며, 균접종 1일째의 결과는 통계적 유의성이 있었다(P<0.05). 3) Interleukin-10/1nterleukin-6 ratio는 실험군과 대조군 모두에서 1일보다 2일째의 결과에서 더 높은 값을 보였으며 대조군에서는 통계적 유의성을 보였다(P<0.05). 4)조직학적 관찰결과 균접종 후 2일째의 조직표본에서는 림프구의 침윤과 부분적인 조직의 괴사 등 염증반응의 양상을 관찰할 수 있었으며, 균접종 5일째의 조직표본에서는 염증의 정도가 감소되는 양상을 확인할 수 있었다.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.12 no.4
• /
• pp.1696-1702
• /
• 2011

This study is to present basic materials to further selection of details of health care service bill and supply resources by analysing the consciousness of radiological technologist and dental hygienist about health care service bill proposed for prevention and early diagnosis of a disease through the improvement of living habit. Subjects were 359 of dental hygienists and radiological technologists investigated from 2010 July to September for three months. As the result, recognition level on health care service bill or its details was less than 30.0% but the opinion that health care service bill is necessary was 78.0%. In its additional item, radiological technologist hope to be maintained currently, and 82.3% of the dental hygienist hope to be added, their prefered additional item was dental disease. A dominant opinion was that dental hygienist and radiological technologist as a service supply personnel were included, and their intention to participate was 8.1 in average. In conclusion, the health care service bill is indispensable, but more systematic study is required based on collecting various opinions about major contents and service provider. Particularly it is inevitable to include the health human resources such as experienced dental hygienist and radiological technologist who had completed the regular course of health education and are having continuous clinical discipline.

• Pediatric Infection and Vaccine
• /
• v.22 no.3
• /
• pp.201-205
• /
• 2015

Acute maxillary sinusitis is a common disorder affecting children. Untreated acute sinusitis can develop into chronic sinusitis, and complications, such as orbital cellulitis or abscess, can occur. Maxillary sinusitis of odontogenic origin is not a well-recognized condition and is frequently missed in children. As an odontogenic source of sinusitis, the dentigerous cyst is one of the most prevalent types of odontogenic cysts, and it is associated with the crown of an unerupted or developing tooth. This report concerns a nine-year-old boy who was diagnosed with maxillary sinusitis originating from a dentigerous cyst with supernumerary teeth. The boy visited our pediatric clinic presenting with rhinorrhea and nasal obstruction and was initially diagnosed with maxillary sinusitis only. With antibiotic treatment, his symptoms seemed to improve, but after 2 months, he came to our clinic with left facial swelling with persistent rhinorrhea and nasal obstruction. Radiographic examinations of the sinuses were performed, and he was diagnosed with maxillary sinusitis originating from a dentigerous cyst with supernumerary teeth. After a surgical procedure involving the removal of the dentigerous cyst with supernumerary teeth, the symptoms of sinusitis gradually diminished. There are only very few cases in the pediatric medical literature that remind us that odontogenic origin can cause maxillary sinusitis in children. Our patient can act as a reminder to general pediatricians to include dentigerous cysts in the differential diagnosis of maxillary sinusitis.

• Journal of Oral Medicine and Pain
• /
• v.31 no.1
• /
• pp.101-111
• /
• 2006

Temporomandibular disorders (TMD) is a collective term embrassing a number of clinical problems that involve the masticatory musculature, the Temporomandibular joint and associated structures, or both. The prevalence of signs and symptoms associated with TMD can be best appreciated by examining epidemiologic studies. But domestic epidemiologic studies about sex, history, chief complaint, diagnosis were not sufficient comparing with foreign countries. The results obtained as follow. 1. The number of visiting patients were 6500 and mean age was 34.06 year (Male's mean age was 33.15, Female's 34.62). In the age of patients, there was no significant difference between sex. 2. There are most patients who were referred by dentist (80.07%). Most diagnostic group was arthrogenous TMD group and followed by myogenous TMD, soft tissue disease. 3. There was strong interaction between diagnostic groups and chief complaint. 4. There was little pain difference between right and left sides. Male mainly had pain onset under 6 month, but female had pain onset more than 6 month. 5. The number of patients who had joint sound history were 3445 (53.15%). There was no significant difference of pain onset between sex. Clicking sound was most among joint sounds which happened to patients. 6. In analyzing the parafunctional habit, male mainly had bruxism, but female had clenching habit.

• Journal of Oral Medicine and Pain
• /
• v.25 no.3
• /
• pp.279-289
• /
• 2000

현대에는 날로 복잡해지는 생활양식의 변화에 따라 다양한 스트레스에 노출되고 그로 인한 면역계, 신경계 또는 각종 장기의 기능이상이 점차로 증가되고 있다. 최근 치과에 내원하는 환자들 중 구강건조증을 호소하는 환자들의 다수는 스트레스에 노출되어 있고, 임상적으로도 방사선 타액선 기능 검사에서 타액선, 특히 악하선의 기능이 현저하게 저하되어 있는 소견을 관찰할 수 있 다. 더욱이 이는 타액선 조영술에서 이미 보고된 바 있는 면역성 질환인 $Sj\ddot{o}gren$ 증후군과는 다른 양상으로 관찰되고 있어, 이에 본인은 백서에 스트레스와 관련이 깊은 내분비적 환경 변화를 유도함으로써 이와같은 변화가 악하선 조직의 병리적 변화와 어떠한 연관성을 갖는 지를 관찰하고자 본 실험을 시행하였다. 생후 7주된 Sprague-Dawley계 웅성 백서(165-209g/bw) 40마리를 2 개의 실험군(부신 제거군 : ADX 군, 부신 제거 후 dexamethasone 투여군 : DEX 군)으로 나누어 실험하였다. ADX 군은 외과적으로 부신을 제거한 후 다른 조건을 부여하지 않았고, DEX 군은 외과적으로 부신을 제거한 후 매일 dexamethasone($1.5*10^{-46}mg/g$ I.V./day)을 투여하였으며, 이들을 각각 실험 후 즉시, 1일, 3일, 5일, 7일에 희생시켰다. 그 후에는 즉시 악하선을 적출하여 면역조직화학법으로 Clusterin의 발현 정도 및 부위를 관찰하였다. 그 결과는 다음과 같았다. 1. 광학현미경 하에서, 양 군 모두 유의할만한 조직학적 변화는 관찰되지 않았다. 2. ADX 군에서는 실험기간 전반에 걸쳐 도관세포와 선포세포에서 clusterin이 발현되었다. 3. DEX 군에서는 소수의 선세포에서 국소적으로 clusterin이 관찰되었으나, 전반적으로 도관세포, 선포세포에서 공히 clusterin이 관찰되지 않았다. 부신을 제거한 군에서는 실험기간 전반에 걸쳐 clusterin이 발현되었는데, 이는 clusterin이 스트레스의 생리적 반응의 결과로서 세포보호작용을 한다는 사실에 기초하여 볼 때, 부신을 제거하였을 때도 스트레스를 받았을 때와 같은 영향이 백서의 악하선에 가해졌을 것으로 생각된다. 반면, 부신제거후 glucocorticoid agonist인 dexamethasone을 투여하였을 때, clusterin이 감소 내지 관찰되지 않았던 것은 부신제거에 의해 악하선에 가해졌던 영향을 dexamethasone이 길항하여 나타난 결과로 볼 수 있어, 스트레스에 의해 부신으로부터 분비되는 glucocorticoid가 타액선을 보호하는데 중요한 작용을 하는 것을 시사한다.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2019.05a
• /
• pp.197-197
• /
• 2019

총인처리시설에서는 응집제를 주입하여 인이나 오염물질을 침전시켜 처리하고 있으며 PAC(poly aluminum chloride)나 $FeCl_3$, $Al_2(SO_4)_3$, 등 다양한 종류의 응집제가 사용되어지고 있다. 하지만 기존에 사용되어 지고 있는 알루미늄계열의 응집제는 처리수에 잔류이온이 존재하게 되어 인체에 축적돼 알츠하이머 병 등 신체질환을 유발할 수 있는 단점이 있다. 따라서 이러한 단점을 사전에 예방하기 위해 $TiCl_4$와 같은 티타늄계열 응집제에 대한 연구가 진행되고 있다. $TiCl_4$ 응집제는 인 제거에 효과적으로 적용할 수 있으며 수중에 잔류이온을 유발하지 않으며 Ti이온은 치과임플란트나 의료장비로 사용될 만큼 인체에 무해하다고 알려져 있다. 응집제 사용에 따라 생성된 응집슬러지의 처리방안에 있어서 기존 응집제의 경우 생성된 슬러지의 대부분은 하수슬러지 위탁처리업체를 통해 소각 및 매립, 재활용 되고 있으나, $TiCl_4$를 응집제로 사용할 경우 생성된 슬러지를 인발하여 건조 및 소성을 통해 이산화티타늄으로 재활용할 수 있어 슬러지를 친환경적으로 처리가 가능해 기존 슬러지 처리방안의 경제적, 환경적 부담을 줄일 수 있는 장점이 있다. 따라서 본 연구에서는 $TiCl_4$를 하수처리장 방류수에 주입하여 수중의 총인(T-P)을 처리한 후 생성된 슬러지를 인발하여 건조와 소성과정을 거쳐 이산화티타늄을 제조하였다. 방류수를 취수해 2.5mg/L의 초기 총인 농도를 가진 원수를 제조하였으며 제조된 원수에 $TiCl_4$를 0.6mL 주입하였을 때 99.93%의 총인제거효율을 얻을 수 있었다. 응집 실험 후 생성된 슬러지를 인발하여 건조 후 $300{\sim}1000^{\circ}C$의 각각 다른 온도조건에서 소성하여 이산화티타늄을 제조하였으며, SEM과 XRD를 통해 제조된 이산화티타늄의 표면특성과 결정성 분석을 실시하였다. 제조된 이산화티타늄은 $500{\sim}800^{\circ}C$에서는 아나타제, $900{\sim}1000^{\circ}C$에서는 루타일 결정구조가 나타났다. 또한, $TiCl_4$ 주입량에 따른 이산화티타늄의 최종 생산량과 제조 시 사용되는 건조로 및 소성로의 경제적 비용 등을 고려하여 이산화티타늄 1Ton을 제조하기 위해 필요한 단가를 산출하였다. 본 연구에서 제조된 이산화티타늄 1kg의 생산단가는 약 5,400원으로 나타났으며, 가장 많이 사용되는 P-25 광촉매 보다 저렴한 가격으로 제조 및 판매를 기대할 수 있다. 따라서 하수처리장에 적용 시 기존 응집제 보다 비싼 $TiCl_4$ 비용을 보완하고 친환경적인 슬러지 처리로 제조된 이산화티타늄의 유통 및 현장적용을 통해 경제적, 환경적으로 우수하다고 판단된다.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.37 no.2
• /
• pp.88-94
• /
• 2021

Purpose: The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment. Materials and Methods: HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay. Results: When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased. Conclusion: NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.

• Journal of Korean Dental Hygiene Science
• /
• v.6 no.1
• /
• pp.65-76
• /
• 2023

Background: The aim of this study was to examine the association between physical activity and periodontitis in the Korean population. Methods: This study utilized data from 9,191 participants of the Korea National Health and Nutrition Examination Survey. Periodontitis was defined as a CPI score of 3 or 4. Physical activity was assessed by the trained interviewer using the International Physical Activity Questionnaire-Short Form (IPAQ-SF). IPAQ-SF is composed of vigorous physical activity, moderate physical activity, and walking activity. Multivariable logistic regression analyses and stratified analyses by obesity were performed. All analyses were conducted separately for males and females. Results: Females who engaged in vigorous and moderate physical activity had a 28% (OR = 0.72, 95% CI = 0.54 - 0.95) and 34% (OR = 0.66, 95% CI = 0.44 - 0.98) lower risk of periodontitis, respectively. In obese females, moderate physical activity in obese had a 65% lower risk of periodontitis (OR = 0.35, 95% CI = 0.18 - 0.67). Conclusions: Our findings suggest that moderate-intensity physical activity was inversely associated with a lower risk of periodontitis. In obese females, moderate physical activity had an independent inverse association with periodontitis.

• Journal of Korean society of Dental Hygiene
• /
• v.23 no.1
• /
• pp.25-31
• /
• 2023

Objectives: This study performed a comparative evaluation of the effects of oil pulling on the periodontal health of smokers. Methods: The experimental (15 subjects) and control (15 subjects) were provided coconut oil and distilled water, respectively. We evaluated the pocket depth (≥4 mm), bleeding on exploration, and Patient Hygiene Performance (PHP) index in both groups following the interventions. Clinical parameters were assessed at baseline, after 4 weeks, and after 8 weeks. Dry mouth and oral health-related quality of life were evaluated at baseline and after 8 weeks. Results: Bleeding on exploring in the control group decreased from 26.17 to 18.33 and from 26.07 to 12.53 in the experimental group (p=0.030), with significant differences in measurement time (p<0.001), and the interaction between group and measurement time (p=0.002). The PHP index in the control group decreased from 24.50 to 16.17 and from 24.00 to 9.83 in the experimental group (p=0.027), with significant differences in measurement time (p<0.001), and the interaction between group and measurement time (p=0.001). Furthermore, the experimental group showed a significant decrease in dry mouth (p<0.001) and a significant increase in oral health-related quality of life (p=0.025). Conclusions: The coconut oil intervention positively affected the periodontal health of smokers.

• Journal of Korea Entertainment Industry Association
• /
• v.13 no.8
• /
• pp.551-561
• /
• 2019

The purpose of this study was to investigate the relationship between entrance exam stress and oral care self-efficacy in girls high school. From June to July 2018, A self-reported questionnaire was administered to 192 high school students in the G area. The data were analyzed for independent t-test, one-way ANOVA and Pearson's correlation coefficient by using SPSS/WIN 21.0 program. Among the sub-sectors of entrance exam stress, exam tension/poor was the highest with 3.07 points, followed by Insufficient spare time stress 2.83 points, future uncertainty stress 2.57 points, and parent pressure stress 2.44 points. the variables related to exam tension/poor stress were academic performance (p<.01), family income level (p<.05), Subjective oral health status(p<.05), and daily brushing frequency(p<.01). The highest level of oral care self-efficacy was 3.13 points for brushing self-efficacy, followed by dental visits 2.80 points and interdental hygiene 2.64 points. As a result of analyzing the general characteristics and oral care self-efficacy, subjects related to brushing self-efficacy were subjective oral health status, caries snacking(daily), and caries drinks(daily)(p<.01). There were negative correlations between entrance exam stress and oral care self-efficacy. The higher the entrance stress, the lower the oral care self-efficacy. To prevent oral disease and increase oral care self-efficacy of students with high entrance stress, it is necessary to provide school oral health education programs that can facilitate oral health behaviors.