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http://dx.doi.org/10.14368/jdras.2021.37.2.88

Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study  

Park, You li (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University)
Kim, Hyun-Joo (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University)
Lee, Ju-Youn (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University)
Jeong, Sung-Hee (Department of Oral Medicine, School of Dentistry and Dental Research Institute, Pusan National University)
Kwon, Eun-Young (Dental Clinic Center, Pusan National University Hospital)
Joo, Ji-Young (Department of Periodontology, School of Dentistry and Dental Research Institute, Pusan National University)
Publication Information
Journal of Dental Rehabilitation and Applied Science / v.37, no.2, 2021 , pp. 88-94 More about this Journal
Abstract
Purpose: The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment. Materials and Methods: HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay. Results: When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased. Conclusion: NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6.
Keywords
periodontitis; non-thermal atmospheric pressure plasma; pilot study; anti-inflammatory; human gingival fibroblast;
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