• Korean Journal of Clinical Laboratory Science
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• v.47 no.2
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• pp.65-70
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• 2015

Antimicrobial agents or additives have commonly been used in domestic animals for the prevention and treatment of bacterial diseases. Unfortunately, this has resulted in the overgrowth of bacteria that is resistant to antimicrobial agents used by humans, and these might get disseminated to humans via the food. In this study, we investigated the prevalence of integrons, and characterized gene cassette arrays in Proteus mirabilis isolates obtained from chickens in Chungcheong province of Korea. Additionally, the correlation between gene cassette arrays and antimicrobial resistance rate was studied. A total of 26 Proteus mirabilis isolates were recovered from chickens in Chungcheong province in Korea. Antimicrobial susceptibility was determined by disk diffusion method. PCR and DNA sequencing were performed to characterize the gene cassette arrays. In addition, we employed repetitive extragenic palindromic sequence-based PCR (REP-PCR) method for clonality analysis of P. mirabilis strains. Of the 26 P. mirabilis isolates tested, 14 (53.8%) isolates carried class 1 integrons, while class 2 and class 3 integrons were not detected in our study. The class 1 integrons harbored genes encoding resistance to aminoglycosides (aacCA5, aadA2, aadA5 and aadA7), trimethoprim (dfrA17, and dfrA32), lincosamides (linF) and erythromycin (ereA). In particular, the presence of class 1 integron had a significant correlatation to a high resistance rate of aminoglycoside and trimethoprim. We confirmed that class 1 integrons are widely disseminated in P. mirabilis isolates from chickens, contributing to the resistance to diverse antimicrobial agents in Korea. To prevent further spreading of antimicrobial resistant genes among P. mirabilis isolates, constant monitoring and clinical policing will become necessary.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.2
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• pp.129-134
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• 2003

This study was conducted to investigate the effect of tall type mixtures(TM) and tall + short type mixtures(TSM) on nutritive utilization. DM intake, digestibility and utilization of nitrogen and energy of herbages from pastures by Korean native goats were determined. This experiment was conducted by total collection method in laboratory, 2000. The experimental herbage included two treatments: Tall type mixtures(orchardgrass, Potomac 40+tall fescue, Fawn 20+Festulolium braunii, Paulita 10+perennial ryegrass. Reveille 10+timothy, Climax 10+red clover, Kenblue 5+alfalfa, Vernal 5%), Tall+short type mixtures {orchardgrass, Potomac 40+tall fescue, Fawn 20+Kentucky bluegrass, New port(turf type) 10+redtop, Barricuda(turf type) 10+perennial ryegrass, Palmer III(turf type) 10+red fescue, Flyer II(turf type) 5+white clover, Regal 5%}. The voluntary dry matter(DM) intake of Korean native goats fed with herbages from tall+short type mixtures(TSM) was slightly higher than that of tall type mixtures(TM), but no difference was observed between TSM and TM(p > 0.05). The digestibility of DM, neutral detergent fiber, cellular contents and acid detergent fiber of herbage from TSM was slightly higher than that of TM, but no difference was detected between TSM and TM(p > 0.05). The utilization of nitrogen and energy by Korean native goats did not showed any difference in herbages from TSM and TM(p > 0.05). In conclusion, the herbage utilization by Korean native goats did not showed any significant difference. Thus, there is a possibility that turf grasses in mixtures can be used as a forage sources.

• Applied Microscopy
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• v.33 no.3
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• pp.223-231
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• 2003

Histology and ultrastructure of the gill in the granular ark, Tegillarca granosa are described using light and transmission electron microscopy. The gill of the clam have typical structure of the filibranch type. The gill filament have several band of lateral and apical cilia. The epithelial layer surrounding the hemolymph sinus is simple and consists of epithelial cells, ciliated cells and secretory cells. The epithelial cells are usually squamous and covered with microvilli. The ciliated cells are usually columnar and can be divided into two types (A and B). Type A cells are more abundance and have lower electron density than B cells. Ultrastructure of the cilia showed that '9+2' microtubular structure of the axial filament and '$2{\times}9$' proximal centriole structure in the cross section. Secretory cells are mainly observed in the apical region of the filament and can be divided into three types of A, B and C with morphological features of the secretory granules. Type A cells of oval shaped are more abundance than other secretory cells and contains numerous secretory granules of low electron dense. Type B cells contains secretory granules of membrane-bounded and high electron dense. Secretory granules of type C cells are elliptical and fine granules surrounding the homogeneous core materials.

• Journal of Life Science
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• v.26 no.5
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• pp.592-602
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• 2016

This study investigated the muscular dystrophin levels in freely moving Australian cattle mainly fed grass, freely moving Korean cattle fed mainly a grain diet, and Korean cattle fed a grain diet but housed in a relatively limited space of a cow house. The total skeletal muscle specimens of 244 cattle were collected and immediately fixed in 10% neutral formalin. The same area was biopsied from the cattle in both countries. The findings showed that fatty infiltration is highly correlated with membrane-associated protein degradation in skeletal muscle, and that among several membrane-associated proteins, dystrophin showed the most significant reduction in expression in the cattle with fatty infiltration. Similarly, CD36 was more highly expressed in the cattle with fatty infiltration of skeletal muscle. Various breeding factors, such as oxidative stress; the presence of oxidized lipids in the diet; and environmental factors such as exercise, temperature and amount of time spent, may have critical effects on the degradation of normal cytoskeleton proteins, which are required for maintaining normal skeletal muscle architecture. Among the sarcolemma membrane-associated proteins, dystrophin is the most sensitive membrane protein that is involved muscular dystrophy and muscular degeneration. Thus, the present findings may be useful for studies on muscular dystrophy in humans or the pathogenesis of muscular diseases in animal models.

• Journal of Animal Environmental Science
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• v.12 no.1
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• pp.1-6
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• 2006

본 연구는 지속 가능한 친환경 축산업의 직접지불제를 도입하는데 농가이행 프로그램 개발과 시범사업의 효과를 분석하기 위하여 전국단위 4대 축종(한우, 젖소, 돼지, 닭)에 대한 2003년도에는 프로그램 개발과 2004년$\sim$2005년(2년간) 시범사업 시행 효과를 분석하기 위하여 각 시 군에 참여한 농가의 현황과 담당 공무원 및 농가에 대한 의향조사를 실시한 결과는 다음과 같다. 1. 기본프로그램(2003년)으로 소(한육우 젖소)는 조사료포 확보 및 분뇨 경지환원, 돼지 닭(산란계 육계)는 사육밀도 완화 및 분뇨발생량 감축을 위하여 발생되는 경지확보 비용 그리고 사육밀도 완화에 따른 감소두수의 소득감소분을 농가부담 50%로 설정하였다. 인센티브 프로그램으로 축사 및 분뇨처리시설 주변에 악취, 조경 등 환경개선을 위하여 조경수를 구입 식재하면 비용의 50%를 직불하도록 하였다. 2. 시범사업 참여실적은 2004년에 459호에 비하여 2005년에 653호로 42.3% 증가하였으며 참여비율은 한우 24.4%, 젖소 17.4%, 돼지 40.2%, 닭 18.0% 이었으며 친환경축산 직불금 지급액 비율 한우 16.1%, 젖소 22.0%, 돼지 46.4%, 닭 15.5% 이었으며 농가당 지급액은 한우 3,987천원, 젖소 7,627천원, 돼지 6,965천원, 닭 5,212천원이었고, 인센티브(조경수 식재) 참여는 전체농가 중 20.4%, 호당지급액 1,303천원(4.6%) 이었다. 3. 조사료포 확보 실적은 한우 $1,303m^2$/두('04)에 비하여 $1,639m^2$/두('05)로 26% 증가하였고, 젖소 $1,517m^2$/두('04)에 비하여 $1,561m^2$/두('05)로 3% 증가하였다. 4. 돼지 닭의 사육밀도는 돼지 $1.14m^2$/두('04)에 비하여 $1.27m^2$/두('05)로 11% 넓어졌으며 산란계는 $0.059m^2$/수('04)에 비하여 $0.083m^2$/수('05)로 41% 넓어졌고 육계는 $0.078m^2$/수('04)에 비하여 $0.069m^2$/수('05)로 12% 좁아졌다. 5. 축산환경개선사업에 대한 설문조사 결과 환경개선제 사용이 악취 및 파리 감소에 효과가 크며 지원사업을 계속해야 한다고 답하였다.

• Korean Journal of Poultry Science
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• v.41 no.1
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• pp.1-5
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• 2014

This study was carried out to investigate the effect of feeding fermented earthworm cast additives (FEC) on egg production, egg yolk fatty acids, blood lipid, cecal microorganism and fecal odor. The completely randomized experiment was applied, and the treatments were designed as 2 feeding regimens (control vs. FEC feeding), and FEC contained 3.5% top dressing to common diet. Egg production and egg weight of FEC group were significantly higher than that of control (p<0.05). Ratios of n-6/n-3 fatty acids in the egg yolk of FEC group were significantly higher than those in control group (p<0.05). Concentrations of HDL-C in FEC group was significantly higher than control group (p<0.05), but not triglyceride and total cholesterol. The caecal Lactobacillus of FEC group was significantly higher in FEC group than control group (p<0.05). $NH_3$ concentrations from poultry house were significantly lower in FEC group than control (p<0.05). In conclusion, these study results showed that the addition of FEC at 3.5% to the diet has reduced odor of poultry house and improved the n-6/n-3 fatty acid of egg yolk and egg production in laying hens.

• Journal of Sericultural and Entomological Science
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• v.29 no.2
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• pp.73-81
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• 1987

This investigation was carried cut to obtain the reeling water conditions in Korea. The analyzed items of water were pH, colority, turbidity, acidity, alkalinity, solids electric conductibity hardness and heavy metal ions. About 40% of the reeling water were found to be agreeable, 30% were acceptable provided with some quality control and the others were out of acceptable range mainly based on the M-alkalinity, acidity, pH and total hardness. It was found that there was a following relationship between total hardness and electric conductibity with the relative correlation coefficient r=0.9145. y=15.967+0.22774x Where x, y are electric conductivity and total hardness respectively.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.11
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• pp.180-186
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• 2020

ICT convergence using smart livestock is that in the first-generation dairy smart farm model, each device made by several manufacturers uses its own communication method, limiting the mutual operation of each device. This study uses a model based on open control technology to secure interoperability of existing ICT devices and to manage data efficiently. The open integrated control derived from this process is the software interface structure of Open API. It is an observer that serves as real-time data collection according to the communication method of ICT devices and sensors located at each end. It consists of a broker that connects and transmits to the upper integrated management server. As a result of the performance analysis through verification of two first-generation dairy smart farm model sites, the average daily milk production increased compared to the previous year (farm A 5.13%, farm B 1.33%, p<0.05). Cow days open (DO) was reduced by 17.5% on farm A and 13.3% for farm B(p<0.05). Cows require an adaptation period after the introduction of the ICT device, but if continuous effects are observed, the effect of production can be expected to increase gradually.

• Journal of Bio-Environment Control
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• v.29 no.1
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• pp.89-95
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• 2020

Livestock facilities in Korea are on their way of enlargement resulting in increment of livestock manure. When the livestock manure treated inappropriate way during application to the agricultural area, environmental damage can be occurred such as bad smell, water and air pollution. Therefore it is important to make a good management plan for livestock manure treatment. In order to effectively apply organic fertilizer made by livestock manure in terms of quantity and quality, the current status of nutrient in agricultural land should be calculated quantitatively by scientific way. The OECD suggest member countries to calculate the nutrient balance as an agricultural environment indicator which represents the difference between nutrient inputs and outputs of the amount of nutrients in various ways. In this paper, we calculated the nutrient balances according to three cities which have different characteristics such as urban or rural areas. To reduce the nutrient balance, we considered how to reduce the amount of chemical fertilizer by using organic fertilizer made by livestock manure. And public treatment facilities for livestock manure can be a good solution except civil complaints for location selection. Among the options, manure fertilizer application to the agricultural area for cultivation of forage crops was mainly considered to reduce the nutrient balance by decreasing input of chemical fertilizers and increasing output of crop production. Using the field monitoring data, it can be calculated that the daily nitrogen reductions by 116.5 kg/ha and the daily phosphorus increments by 2.7 kg/ha.

• Economic and Environmental Geology
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• v.45 no.1
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• pp.23-30
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• 2012

Major ions and nitrogen isotopic analyses were performed to determine the geochemical characters and to identify the source of nitrate of the shallow groundwater around agricultural field in the Youngdong area. The pH value of groundwater ranges from 60. to 8.2 (pH 7.2, mean). The average of EC, Eh and DO is 369 ${\mu}S/cm$ (70~729 ${\mu}S/cm$), 165.6 mV (29~383.2 mV), 4.3 mg/L (1.8~8.0 mg/L) respectively. The ion concentraion of groundwater was in the order of $Ca^{2+}$>$Na^{2+}$>$Mg^{2+}$>$K^{2+}$ and ${HCO_3}^-$>${NO_3}^-$>${SO_4}^{2-}$>$Cl^-$>$F^-$. Most of groundwater is Ca-$HCO_3$ type. The groundwater was affected by water-rock interaction in the shallow depth. Some groundwater is Ca-Cl or Na-$HCO_3$ (2.5%) type that was due to agricultural activities. The $NO_3$_N concetration of grondwater range from 10.2 mg/l to 26.9 mg/l, which show that this area is under nitrate pollution. ${\delta}^{15}N-NO_3$ value of the groundwater is the origins of are a combination of animal wastes and man-made fertilizers.