• Title/Summary/Keyword: 최적pH

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Optimum pH of Chicken peroxidase (닭의 과산화수소 분해효소의 최적pH)

  • Lee, Beom-Gyu;Kwon, Hyo-Shik;Kim, Kyoung-Eun;Yoon, Kil-Joong
    • Analytical Science and Technology
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    • v.14 no.3
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    • pp.286-289
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    • 2001
  • The effect of pH and the substrate concentration on the sensing ability of the chicken peroxidase enzyme electrode was examined quanititatively. Using the new Michaelis-Menten equation, to which pH concept was introduced, enabled to calculate all kinds of dissociation constant related to chicken peroxidase and subsequently to determine the optimum pH of the sensor.

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Study on the Hemolysin from Marine V. vulnificus (해양 V. vulnificus의 Hemolysin에 관한 연구)

  • 이봉헌;박흥재
    • Journal of Environmental Science International
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    • v.6 no.3
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    • pp.225-229
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    • 1997
  • A halophilic V. vulnificus is an estuarine microorganism that has been associated with fatal wound Infection and life-threatening septicemia. Hemolysin is defined as toxic substance produced by various species of bacteria Including V. vulnificus. Hemolysin from marine V. vulnificus was purified and the effect of pH, temperature. metal ion on the activity of hemolysin, and thermostability of hemolysin were tested in this study. Hemolysin iysed the sheep red blood cell and the optimum pH was 8.0, the optimum temperature was 4$0^{\circ}C$, and $K^+$ increased but $Mn^{2+}$ decreased the hemolyic activity of hemolysin, but hemolysin was unstable to heat.

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Fenton Oxidation of Landfill Leachate by $Fe^0,\;Fe^{2+},\;Fe^{3+}\;/\;H_2O_2$ Systems ($Fe^0$, $Fe^{2+}$, $Fe^{3+}$ / $H_2O_2$ 시스템을 이용한 침출수의 Fenton 산화반응)

  • Park, Sung-Ho;Han, Ihn-Sup
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.4
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    • pp.402-408
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    • 2005
  • This investigation aimed at selecting the optimum catalyst and reaction conditions used in Fenton oxidation for landfill leachate treatment and was carried out at ambient temperature using a lab-scale experiment. The investigation led to the following results: 1) The optimum pH and dose for each iron catalyst were as follows: $Fe^{2+}\;=\;1,200\;mg/L$, $H_2O_2\;=\;1,200\;mg/L$, initial pH=3.0; $Fe^{3+}\;=\;1,200\;mg/L$, $H_2O_2\;=\;1,500\;mg/L$, initial pH=4.5; $Fe^0\;=\;1,200\;mg/L$, $H_2O_2\;=\;900\;mg/L$, initial pH=4.0, respectively. 2) The progress of Fenton oxidation could be instrumentally monitored by measuring redox potential evolution during leachate oxidation, thus, indicating the possibility of an on-line process monitoring. 3) A simple acid-base titration of Fenton-treated leachate proved that a relevant fraction of by- products formed during the treatment was made of acidic compounds in the optimum reaction condition for each catalyst used, thus demonstrating that the higher the extent of Fenton oxidation the greater was the amount of acids formed. 4) With the aim of selecting the optimum catalyst among $Fe^0$, $Fe^{2+}$ and $Fe^{3+}$, removal efficiency of each parameter in the optimum reaction conditions was considered. Although $Fe^{3+}$ was higher than other catalysts($Fe^0$, $Fe^{2+}$) in removal efficiency, $Fe^0$ was a optimum catalyst with a view of cost effectiveness.

The Immobilization Characteristics of Thiobacillus sp. IW (Thiobacillus sp. IW의 고정화특성에 관한 연구)

  • 김성미;오광중김동욱
    • KSBB Journal
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    • v.11 no.6
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    • pp.649-653
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    • 1996
  • Imnmobilization characteristics of Thiobacillus sp. IW which oxidizes sulfur compound was studied to use the bacterium in odor controlling equipment for the future. The optimum growth conditions for Thiobacillus sp. IW were pH7, $30^{\circ}C$ and the generation time was 38min, which was extremely fast compared with other sulfur oxidizing bacteria. Optimum growth conditions in activated carbon as a carrier was pH5, $35^{\circ}C$ and those in bioceramics was pH 7∼8, $35^{\circ}C$. Cell growth immobilized in bioceramics was more stable in pH, temperature change than that immobilized in activated carbon and total number of cells in bioceramics were also higher. Based on these results, the bioceramics is thought to be better carrier in immobilization of Thiobacillus sp. IW.

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(J,J')-lossless factorization and $H^{\infty}$ control in discrete-time systems (이산시간 시스템에서 (J,J')-lossless 분해와 $H^{\infty}$ 제어)

  • 정은태;이재명;박홍배
    • Journal of the Korean Institute of Telematics and Electronics B
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    • v.31B no.5
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    • pp.65-72
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    • 1994
  • We resolve the suboptimal $\infty$ control problem using (J,J')-lossless coprime factorization by transforming the linear fractional transformation (LFT) into chain scattering description (CSD) in discrete-time systems. The condition transformed LFT into CSD is that the inverse matrix of $P_{21}$ of standard plant exists. But, this paper presents the method of transforming LFT into CSD for 4-block problem in case that the inverse matrix of $P_{21}$ of standard plant does not exist and parameterization of the all suboptimal $\infty$T controllers using (J,J')-lossless coprime factorization. It is shown that this method can resolve the suboptimal $\infty$ control problem solving only two Riccati equations in discrete-time systems.

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Studies on Cellulolytic Enzymes Produced by Chaetomium globosum -Part . 1 ; Properties of Crude Cellulolytic Enzymes- (Chaetomium globosum 이 생성(生成)하는 Cellulose 분해효소(分解酵素)에 관(關)한 연구(硏究) -제1보(第1報) 조효소(粗酵素)의 성질(性質)-)

  • Chung, Dong-Hyo
    • Applied Biological Chemistry
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    • v.10
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    • pp.23-31
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    • 1968
  • We have obtained the following results, at the production of cellulase of Chaetomium globosum and its properties of crude enzyme. 1. At the production of enzyme, wheat bran solid culture was more active than surface or shaking culture. 2. The production of enzyme was maximum between the eighth and the tenth days, but slightly decreased thereafter. 3. The optimum condition of the reactions in saccharification with CMC were obtained the following results. 1) The optimum pH was within the range of from 4.0 to 5.0 and stable pH range was within 3.5 to 6.5. 2) The optimum temperature was $40^{\circ}C$ and thermal stability was below $50^{\circ}C$ and completely inactivated at $70^{\circ}C$ 4 Dialyzed crude enzyme was activated by $Mn^{++}\;Mg^{++}\;Fe^{++}\;and\;Mo^{++}\;respectively\;but\;Hg^{++}$ was inhibited its enzyme action.

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Purification and Characterization of Proteases from Streptomyces sp. SMF301 (Streptomyces sp. SMF301에서 분리한 단백질 분해효소의 성질)

  • Jeong, Byeong Chul;Hyun Seung Shin;Kye Joon Lee
    • Microbiology and Biotechnology Letters
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    • v.16 no.6
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    • pp.526-531
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    • 1988
  • Procedure for the purification of pretense from culture broth of Streptomyces sp. SMF301 was developed. It was evident that the strain produced two different proteases of which molecular weights were estimated to be 23, 500 and 38, 900 dalton. It was found that the optimum pH of the smaller was 9.0 and that of the larger was 1.0. The optimal temperature of the alkaline pretense was 5$0^{\circ}C$ and that of the neutral pretense was much more stable than neutral protease at extreme condition viz. high temperature, and pH.

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Effect of Surfactant Concentration and pH on Surfactant-Enhanced Remediation in lowa Soil Contaminated by TCB (삼염화벤젠으로 오염된 아이오와토양의 복원시 계면활성제의 농도와 pH의 영향)

  • Dal-Heui Lee;Robert D. Cody
    • Economic and Environmental Geology
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    • v.35 no.2
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    • pp.149-154
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    • 2002
  • Column tests were carried out to examine the effect of surfactant solution conditions on surfactant-enhanced remediation of contaminated soil. The selected conditions of the surfactant solution were concentration and pH. 1,2,4-trichlo-robenzene (TCB) was chosen as the model hydrophobic organic substances. Sodium diphenyl oxide disulfonate (DOSL) and octylphenoxypoly ethoxyethanol (OPEE) surfactants were selected for this study. Two Iowa soils, Fruitfield sand and Webster clay loam, were leached with surfactant solution. The test results revealed that an optimum condition was achieved for 4 %(v/v) of concentration and 10 of pH, respectively. The maximum recoveries of added TCB (93-98%) were obtained when optimal conditions of each surfactant solution parameter were simultaneously met. The optimum conditions of these parameters may be useful for surfactant-assisted remediation in soil contaminated by TCB.

Optimization of Submerged Culture Conditions for the Production of Ginseng Root Using Response Surface Method (반응표면분석법을 이용한 인삼 Root 액체배양조건의 최적화)

  • 오훈일;장은정;이시경;박동기
    • Journal of Ginseng Research
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    • v.24 no.2
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    • pp.58-63
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    • 2000
  • To develop the production of ginseng root using plant tissue culture technology, submerged culture conditions were optimized by means of the fractional factorial design with 4 factors and 3 levels by a RSM computer program. The ginseng (Panax ginseng C. A. Meyer) roots induced by plant growth regulators were cultured on SH medium and the effects of various pH of medium, sucrose concentration, nitrogen concentration and phosphate concentration on fresh weight of the ginseng root were investigated. The fresh weight of ginseng root increased with a decrease in nitrogen concentration and fresh weight of ginseng root varied from 1.00 to 2.33g under various conditions. The optimum pH of medium and sucrose concentration determined by a partial differentiation of the model equation, nitrogen and phosphate concentration were pH 5.6, sucrose 3.8%, nitrogen 50 mg/L and phosphate 80.7 mg/L, respectively. Under these conditions, the predicted growth of ginseng root was estimated to be 2.36g.

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Studies on the Hydrolysis of Milk Fat by Microbial Lipases (미생물에서 추출된 Lipase의 유지방 분해)

  • Park, Jong-Hack;Lee, Young-Chun
    • Korean Journal of Food Science and Technology
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    • v.17 no.2
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    • pp.60-64
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    • 1985
  • To utilize microbial lipases for hydrolysis of milk fat, optimum reaction conditions and characteristics of enzymatic reactions of lipases originated from Rhizopus delemar, Mucor sp., and Candida cylindracea were investigated. Optimum pH and temperature were pH 5.6 and $45^{\circ}C$ for Rhizopus delemar lipase, pH7.5 and $35^{\circ}C$ for Mucor sp. lipase, and pH7.5 and $35^{\circ}C$ for Candida cylindracea lipase. Optimum lipase concentration and optimum substrate concentration were $600{\sim}800\;units/ml$ and 20% milk fat, regardless of their origin. Km values were 6.06% milk fat for Rhizopus delemar lipase, 7.69% for Mucor sp. lipase and 7.99% for Candida cylindracea lipase. Rate of lipid hydrolysis was Rhizopus delemar lipase>Mucor sp. lipase>Candida cylindracea lipase. As the reaction time was extended, liberation of short chain fatty acids was increased. After 8 hours reaction, capric acid content significantly increased with Candida cylindracea lipase, palmitic acid with Mucor sp. lipase and butyric acid with Rhizopus delemar lipase.

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