• Title/Summary/Keyword: 최적 성장조건

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Effect of Culture Conditions on the Growth Characteristics and Survival of Bifidobacterium breve (Bifidobacterium breve의 성장 특성과 생존력에 미치는 배양조건의 영향)

  • Park, Hee-Kyung;Heo, Tae-Ryeon
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.451-457
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    • 1996
  • The effects of pH and L-cysteine HCI on the growth and stability of Biofidobacterium breve were studied. Significantly higher population was obtained by culturing at pH $6.0{\sim}6.5$ than at any other pH. The cultures that had been grown at pH $5.5{\sim}6.0$ were more stable during storage than those grown at other pH. The number of B. breve that had been grown at pH 5.5 and 6.0 remained as $2.4{\times}10^6ml/\;and\;1.4{\times}10^6ml,$ respectively, after 25 days of storage at $4^{\circ}C$. The ${\beta}$-galactosidase activity of B. breve grown at pH 5.5 and 6.0 was reduced only to $78{\sim}85%$ of the control after the same storage condition, whereas the culture grown at pH 7.0 exhibited a signficant decline in population and ${\beta}$-galactosidase activity during $4^{\circ}C$. The growth of B. breve was promoted by 0.05% L-cysteine HCI, and cells grown in MRS with $0.05{\sim}0.10%$ L-cysteine HCI were more resistant to hydrogen peroxide. With respect to the effect to the effect of osmoprotectants on the survival of B. breve subjected to freeze-drying, addition of 2 mM betaine of 2 mM trehalose increased the growth rate of cells grown under osmotic stress and also made the organism more osmotolerant. Furthermore, the betaine or trehalose increased the survivability of the cells after freeze-drying.

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Growth and photocurrent properties for ZnIn2S4 single crystal thin film by Hot Wall Epitaxy method (Hot Wall Epitaxy (HWE)법에 의한 ZnIn2S4 단결정 박막 성장과 광전류 특성)

  • 박창선;홍광준
    • Proceedings of the Materials Research Society of Korea Conference
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    • 2003.11a
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    • pp.156-156
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    • 2003
  • 수평 전기로에서 ZnIn$_2$S$_4$ 다결정을 합성하여 HWE(Hot Wall Epitaxy)방법으로 2nIn2S4단결정 박막을 반절연성 GaAs(100)기판 위에 성장시켰다. ZnIn2S4 단결정 박막은 증발원의 온도를 610 $^{\circ}C$, 기판의 온도를 450 $^{\circ}C$로 성장시켰고 성장 속도는 0.5 $\mu\textrm{m}$/hr로 확인되었다. ZrIn2S4 단결정 박막의 결정성의 조사에서 10 K에서 광발광(photoluminescence) 스펙트럼이 433 nm (2.8633eV)에서 exciton emission스펙트럼이 가장 강하게 나타났으며, 또한 이중결정 X-선 요동곡선(DCRC)의 반폭치(FWHM)도 133 arcsec로 가장 작아 최적 성장 조건임을 알 수 있었다. Hall 효과는 van der Pauw방법에 의해 측정되었으며, 온도에 의존하는 운반자 농도와 이동도는 293K에서 각각 8.51$\times$$10^{17}$ electron/$cm^{-3}$ 291 $\textrm{cm}^2$/v-s였다. ZnIn2S4 단결정 박막의 광전류 단파장대 봉우리들로부터 10 K에서 측정된 $\Delta$Cr(crystal field splitting)은 0.1678 eV, $\Delta$So(spin orbit coupling)는 0.0148 eV였다. 10 K의 광발광 측정으로부터 고품질의 결정에서 볼 수 있는 free exciton 과 매우 강한 세기의 중성 주개 bound exciton등의 피크가 관찰되었다. 이때 중성 주개 bound exciton의 반치폭과 결합 에너지는 각각 9 meV와 26 meV 였다. 또한 Haynes rule에 의해 구한 불순물의 활성화 에너지는 130 meV 였다.다.

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Cultivation of Chlorella Sp. Using Light Emitting Diode (발광다이오드를 이용한 클로렐라 배양 연구)

  • Lee, Tae-Yoon;Choi, Bo-Ram;Lee, Jea-Keun;Lim, Jun-Heok
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.8
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    • pp.591-597
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    • 2011
  • The purpose of this study was to determine optimum conditions for the cultivation of Chlorella sp. FC-21 using light emitting diodes (LEDs). Specific growth rate and cell concentration were measured for the reactors at the illuminations of different wavelengths of LEDs. Among various types of LEDs, red LEDs were the most effective light source, and also greatest increases of specific growth rate and cell concentrations were obtained when light intensity of red LEDs increased. The specific growth rate decreased when initial cell concentration increased due to the shading effect of each cell in the reactor. To determine beneficial effect of aeration to cell cultivation, micro-air bubbles were aerated at 0.35 vvm in the reactor at the illumination of red LEDs. Two and ten times greater specific growth rate and cell concentration were obtained when aeration was applied. From this study, we found that red LEDs with aeration were the most appropriate light source for the cultivation of Chlorella sp. FC-21.

XPS study of NiO Growth on Ag(100) (Ag(001)에 성장된 NiO 극초박막의 화학 결함 연구)

  • Yang, Seol-Un;Seong, Shi-Jin;Kim, J.S.;Hwang, Han-Na;Hwang, C.C.;Chang, Young J.;Park, Soo-Hyon;Min, H.G.
    • Journal of the Korean Vacuum Society
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    • v.16 no.5
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    • pp.311-321
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    • 2007
  • We have researched the chemical defects of NiO ultrathin films grown on Ag(001) by x-ray photoelectron spectroscopy. In particular, O 1s and Ni 2p spectra were analyzed consistently with control film thickness, $O_2\;and\;H_2O$ partial pressure and substrate temperature. As a result, we could identify each chemical defect. In addition, we suggest the optimum growth condition to minimize the defect density.

Effects of cultural conditions on growth of Micrococcus sp. and casein hydrolysis : (I) Studies on compositions of media (배양조건이 Micrococcus sp.의 생육 및 casein 분해에 미치는 영향 : (I) 배지조성에 관한 연구)

  • Lee, Si-Kyung;Joo, Hyun-Kyu;Pek, Un-Hau
    • Applied Biological Chemistry
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    • v.34 no.4
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    • pp.327-333
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    • 1991
  • This study was carried out to determine the effects of media compositions on cell growth and casein hydrolysis for cell production in order to add Micrococcus sp. LL3 as a potential agent for industrial application for the shortening of ripening period. Monosaccharides like glucose, mannose and fructose were mare excellent as carbon source, but arabinose and xylose markedly inhibited cell growth and caseinolysis. Among the organic nutrients, yeast extract was more effective for cell growth and for caseolysis. However, inorganic nitrogen sources were less effective than organic sources. Urea inhibited cell growth severely. Cell growth and caseinolysis were rather increased a little in the broth containing 1% NaCl, and the organism tolerated and grew in relatively high concentrations of NaCl up to 9%. Addition of vitamin did not affect cell growth and caseolysis in level of $0.1\;{\mu}g/ml$ concentration. Cell growth and caseinolysis were stimulated by addition of glutamic acid and $MgSO_4$ with concentration of 0.2% and 0.05% respectively.

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A Method of Determination of the Number of Tests for Reliability Growth Management (신뢰성 성장관리 시험의 시험 시료 수 결정 방안)

  • Yangwoo Seo;Daeung Choi;Chunsup Um;Yonggeun Kim;Jungtae Kim
    • Journal of The Korean Institute of Defense Technology
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    • v.5 no.1
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    • pp.1-6
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    • 2023
  • The number of test samples was calculated by setting the reliability growth management test period considering the weapon system development period. The optimal reliability growth management test design condition was 80% reliability, 60% confidence level, and 6 months of test period. At this time, it was analyzed that 4 test samples were required if 0 failure occurred, and 9 test samples were required if 1 failure occurred. Using the method of determining the number of samples presented in this paper, it can be used as a basis for acquiring a budget for the number of samples for reliability growth management when switching from the exploratory development stage to the system development stage.

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Induced Breeding and Indoor Culture of the Catfish, Silurus asotus (Teleostomi : Siluridae) (메기(Silurus asotus)의 산란 유도 및 실험실 사육에 관한 연구)

  • CHOI Gyeong Cheol;KIM Dong Soo;JO Jae-Yoon;KIM Jong-Man
    • Journal of Aquaculture
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    • v.5 no.2
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    • pp.117-126
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    • 1992
  • Induced breeding and indoor rearing of the catfish, Silurus asotus were performed. Ovulation was induced by injection of 5 mg dried carp pituitary per kg body weight. Fertilization rates were $81.5\~98.0{\%}$ and hatching success was $67.0\~82.0{\%}$. There were no significant differences of daily growth rate (DGR) and food conversion ratio (FCR) between the groups fed extruded eel pellet and extruded carp pellet. No significant differences in DGR and FCR were also found between $3{\%}$ and $4{\%}$ daily feeding rates. DGR and FCR of small fry were slightly affected by dissolved oxygen (DO) level in aquaria. When the DO level maintained at $4.5\;mg/{\ell}$, the best result was obtained and DGR and FCR were $5.7{\%}$ and 0.65, respectively. At the higher level of DO, $5.5\;mg/{\ell}$, DGR and FCR were $5.52{\%}$ and 0.77, respectively, whereas at the lower level of DO, $3.5\;mg/{\ell}$, the DGR was decreased to $3.45{\%}$ and FCR was increased up to 1.01.

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Characterization of alpha-Ga2O3 epilayers grown on cone-shape patterned sapphire substrate by halide vapor phase epitaxy (원뿔 형태의 patterned sapphire substrate 위에 성장한 α-Ga2O3의 특성분석)

  • Son, Hoki;Choi, Ye-Ji;Lee, Young-Jin;Kim, Jin-Ho;Kim, Sun Woog;Ra, Yong-Ho;Lim, Tae-Young;Hwang, Jonghee;Jeon, Dae-Woo
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.29 no.4
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    • pp.173-178
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    • 2019
  • In this study, we demonstrated a characterization of ${\alpha}-Ga_2O_3$ grown on a cone-shape patterned sapphire substrate by using the halide vapor phase epitaxy. An ${\alpha}-Ga_2O_3$ was grown on different size of PSS and c-plane sapphire substrate for comparison to confirm the effect of PSS. In addition, growth time of ${\alpha}-Ga_2O_3$ was gradually increased to confirm growth mechanism of ${\alpha}-Ga_2O_3$ grown on the PSS. A growth temperature was changed to $470-550^{\circ}C$. It can be analyzed growth conditions and mechanisms on the cone-shape PSS, resulting in a significant decrease in the FWHM value of an asymmetric plane (10-14) of ${\alpha}-Ga_2O_3$, due to lateral growth that occurs during the growth process.

Phylogentic Position, Pigment Content and Optimal Growth Condition of the Unicellular Hydrogen-Producing Cyanobacterial Strains from Korean Coasts (한국 연안산 단세포성 수소생산 남세균 종주들의 분류계통, 색소함량 및 최적성장 환경)

  • PARK, JONG-WOO;KIM, JU HEE;CHO, AE-RA;JUNG, YUN-DUK;KIM, PYOUNG JOONG;KIM, HYUNG-SEOP;YIH, WONHO
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.20 no.3
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    • pp.131-140
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    • 2015
  • To set up unicellular cyanobacterial strains with photo-biological $H_2$ production potential, live samples were repeatedly collected from 68 stations in the coastal zone of Korea for the four years since 2005. Among 77 cyanobacterial strains established six (KNU strains, CB-MAL002, 026, 031, 054, 055 and 058) were finally chosen as the excellent strains for $H_2$ production with $H_2$ accumulation over 0.15 mL $H_2\;mL^{-1}$ under general basic $H_2$ production conditions as well as positive $H_2$ production for more than 60 hr. To explore optimum procedures for higher $H_2$ production efficiency of the six cyanobacterial strains, the inter-strain differences in the growth rate under the gradients of water temperature and salinity were investigated. The maximum daily growth rates of the six strains ranged from 1.78 to 2.08, and all of them exhibited $N_2-fixation$ ability. Based on the similarity of the 16S rRNA sequences, all the test strains were quite close to Cyanothece sp. ATCC51142 (99%). The six strains, however, were grouped into separate clades from strain ATCC51142 in the molecular phylogeny diagram. Chlorophyll- a content was 3.4~7.8% of the total dried weight, and the phycoerythrin and phycocyanin contents were half of those in the Atlantic strain, Synechococcus sp. Miami BG03511. The growth of the six strains was significantly suppressed at temperatures above the optimal range, $30{\sim}35^{\circ}C$, to be nearly stopped at $40^{\circ}C$. The growth was not inhibited by high salinities of 30 psu salinity in all the strains while strain CB055 maintained its high growth rate at low salinities down to 15 psu. The euryhaline strains like CB055 might support massive biotechnological cultivation systems using natural basal seawater in temperate latitudes. base seawater. The biological and ecophysiological characteristics of the test strains may contribute to designing the optimal procedures for photo-biological $H_2$ production by unicellular cyanobacteria.

Numerical analysis of CZ growth process for sapphire crystal of 300 mm length: Part I. Influence of hot zone structure modification on crystal temperature (300 mm 길이의 사파이어 단결정 대한 CZ성장공정의 수치해석: Part I. 핫존 구조 변경이 결정 온도에 미치는 영향)

  • Shin, Ho Yong;Hong, Su Min;Kim, Jong Ho;Jeong, Dae Yong;Im, Jong In
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.23 no.6
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    • pp.265-271
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    • 2013
  • Czochralski (CZ) growth process is one of the most important techniques for growing high quality sapphire single crystal for LED application. In this study, the inductively-heated CZ growth processes for the sapphire crystal of 300 mm length have been analyzed numerically using finite element method. The hot zone structures were modified with the crucible geometry change and the additional insulation layer installed above the crucible. The results show that the solid-liquid interface height decreased from about 80 mm at initial stage to 40 mm after mid-stage due to achieve the growth speed balance. Also the optimal input power of the modified system was similar with the original one due to the compensation effects of the crucible geometry and additional insulation. The crystal temperature grown by the modified CZ grower was increased about 10 K than the original one. Therefore the sapphire crystal of 300 mm height was grown successfully.