• Title/Summary/Keyword: 최적환경조건

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Isolation of Marine Bacteria Killing Red Tide Microalgae -III. Algicidal Effects of Marine Bacterium, Micrococcus sp. LG-5 against the Harmful Dinoflagellate, Cochlodinium polykrikoides- (적조생물 살조세균 탐색 -III. 유해성 적조생물 Cochlodinium polykrikoides에 대한 Micrococcus sp. LG-5의 살조 효과-)

  • JEONG Seong-Youn;PARK Young-Tae;LEE Won-Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.4
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    • pp.331-338
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    • 2000
  • The algicidal effects of marine bacteria were investigated and a strain, which had the strongest algicidal activity against the harmful dinoflagellate, Cochiodinim polykrikoides was selected. The bacterium was isolated in seawater during the period of blooming of C. polykrikoides in Masan Bay. This algicidal bacterium was identified as Micrococcus sp. LG-5 by means of morphological and biochemical tests. The optimal culture conditions of Micrococcus sp, LG-5 were $25^{\circ}C,\;pH 7.0\;and\;3.0{\%}$ NaCl concentration. The algicidal activity of Micrococcus sp. LG-5 was significantly increased to maximum value in the late of logarithmic phase of cell cuture. In addition, the culture filtrate ($pore size,\;0.1{\mu}m$) of Microcoocus sp. LG-5 showed strong algicidal effects. The cell numbers of C. polykikoides were decreased from $1.2{\times}10^4 cells/ml\;to\;less\;than\;2{\times}10^3\;cells/ml$ within 3, 6, 30 hours at the concentrations of culture filtrate $10{\%},\;5{\%}\;and\;1{\%}$, respectively. These results indicated that the algicidal effect was mediated by certain substances released from Microooccus sp. LG-5.

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Microencapsulation of Aster scaber and Aster glehni by Spyay Drying (분무건조법을 이용한 참취 및 섬쑥부쟁이 추출물의 미세캡슐화)

  • 강윤창;최경구;김공환;김현구
    • Food Science and Preservation
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    • v.9 no.2
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    • pp.212-220
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    • 2002
  • Spray drying was used a means for microencapsulation of Aster scaber and Aster glehni. The optimum conditions of spray drying were found to be 210$^{\circ}C$ for the inlet temperature and 5 $m\ell$/min for the feeding rate. The color difference decreased by 12∼25 % far the herb extracts depending on storage temperatures. With an increase in storage temperature, color of the control changed considerably compared to microencapsulated powders. This is due to the fact that wall materials minimized effect of oxygen on the herb extracts. Microencapsulated powders in the amounts of 50 ppm and 250 ppm added to hydrogenated soybean oils led to lower acid values and conjugated diene values during 6 day storage. Compared to the control, the herb extracts displayed 16∼48% increase in antioxidation effect, while the microencapsulated powders exhibited 46∼88% increase. Extracts also showed lower conjugated dienoic acid contents than those of the control. Although antioxidation effect of the microencapsulated powders did not prevail on the significant level in the early stage of storage of oils tested, it became predominant after one week of storage as wall materials were gradually degraded under the high temperature thereby releasing core material from the microcapsule.

Factors Regulating the Nitrogen Fixation Activity and Growth of Anabaena variabilis ATCC 29413 (Anabaena variabilis ATCC 29413 의 생장과 질소고정활성의 조절요인)

  • 송승달;한동훈
    • Korean Journal of Microbiology
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    • v.30 no.5
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    • pp.391-396
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    • 1992
  • Anabaena variabilis A TCC 29413. a photoautotrophic and nitrogen fixing cyanobacteria. was investigated on the environmental factors regulating the growth and nitrogen lixation activity. A good growth of cyanobacteria] cells was observed due to nitrogen t1xation by the heterocyst differentiation in nitrogen free Allen and Arnon (]/8) medium. The nitrogenase activity was appeared to be in proportion to the cell growth lor 6 days then drastically decreased in the later growth period when the nitraTe was accumulated to high level in the culture to cause the inhibition. The optima] conditions lilr the cell growth and nitrogenase activity of A. varillbili.l were anaerobic. IO.OO0 lux. $30^{\circ}C$ and pH 8 with the nitrogen Cree minimal medium. The activity was significantly inhihited by the low concentrations of ammonium and nitrate. but was stimulated b) the ]ow Ieve] of phosphate and carbonate sources. The treatments of several toxic heavy metals showed strong inhibition of the cell growth and nitrogenase activity by O.3~10 ppm in the order of $Hg^{2+}$ > $Cd^{2+}$ > $Co^{2+}$ > $Zn^{2+}$ > $Ph^{2+}$, and the concentrations for 50% inhibition of the maximum activity were 0.41. 0.47. 0.5 L 0.66 and 8.1 ppm. respectively. The addition of carbohydrates (0.5~ 1.0%) in the dark condition stimulated the growth and activity in the order of sucrose > fructose > glucose.

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Performance of Rotating Biological Contactor under Various Hydraulic Residence Time on thle Removal of Total Ammonia Nitrogen and COD in a Simnulated Water Recirculating System (모의 순환여과식 실험장치에서 회전원판반응기 (RBC)에 의한 순환수처리)

  • SUH Kuen-Hack;KIM Byong-Jin;LIM Sung-Il;CHO Jin-Koo;KIM Yong-Ha;OH Chang-Sup
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.32 no.2
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    • pp.180-185
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    • 1999
  • Rotating Biological Contactor (RBC) was tested for the treatment of artificial rearing water in n simulated aquaculture system. Performance of RBC on the removal of TAN and COD was evaluated by controlling hydraulic residence time (HRT). As HRT of RBC was increased, TAN removal rate ana removal efficiency of RBC and TAN concentration of rearing water were increased, but COD removal rate was decreased. Total alkalinity consumption rate was increased by increasing HRT of RBC. Ratio between total alkalinity consumption rate and TAN removal rate was 7.73. HRT for maintaining lowest TAN and COD concentration of artificial rearing water was 14,6 minutes and at that condition TAN and COD concentration of the water was 1.28 and $5.59 g/m^3$, respectively.

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Scheduling Method of Real-Time Mobile Transaction Manager considering Value of Transactions and Validity of Real-Time Data (트랜잭션의 중요도와 데이터의 유효성을 고려한 실시간 이동 트랜잭션 관리자의 스케줄링 기법)

  • Jo, Suk-Gyeong;Kim, Gyeong-Bae;Lee, Sun-Jo;Bae, Hae-Yeong
    • The KIPS Transactions:PartD
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    • v.8D no.5
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    • pp.533-542
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    • 2001
  • In this paper, we present a scheduling method for real-time mobile transaction manager in mobile computing environment. The proposed method checks whether a transaction is executable or not. It is able to by considering not only the deadline of real-time data in mobile hosts. And then, it schedules the real-time mobile transactions by making optimal execution window based on the priority queue, while considering transaction value and deadline. Disconnection with mobile hosts is monitored in selecting the transaction for execution. Using the proposed method reduces the number of restarting times after transaction aborts which is caused by the violation of the validity constraints of real-time data. And in has merits of maximizing the sum of values of real-time mobile transactions which meet the deadline. The performance evaluation demonstrates that the number of committed real-time transactions within the deadline is improved by 20%. This method can be used in real-time mobile transaction manager is such environments as cellular communications, emergency medicine information system and so on.

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A Study of the Growth Characteristics of Starry Flounder Platichthys Stellatus in Accordance with the LED Wavelength (LED 파장에 따른 강도다리 Platichthys Stellatus 성장특성)

  • Jang, Jun-Chul;Her, In-Sung;Lee, Se-Il;Yu, Young-Moon
    • Journal of Advanced Marine Engineering and Technology
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    • v.39 no.4
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    • pp.495-500
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    • 2015
  • Currently the fish aquaculture industry of Korea is focused on the mass culture of flatfish (Paralichthys olivaceus) and and rockfish (Sebastes schlegeli) with completely controlled culture techniques. Recently, there has been considerable interest in new species development, such as the starry flounder (Platichthys stellatus). The value of starry flounder (Platichthys stellatus) as a raw fish increases with time because it is tasty, light, and bouncy. In this paper, the growth characteristics dependent on the LED wavelengths and the optimal growth conditions of the starry flounder were studied. In these experiments 4 different kinds of LED lighting, configurations were designed and prepared using red, green, blue and white, respectively. The fish aquaculture experiments were conducted over 10 weeks in four fish tanks, each installed with a different color of LED lighting. 10 starry flounders of 13 ~ 17g were placed into each tank. The effects of each color of light on the growth rate of the starry flounders were then examined. As a result, the starry flounders under the green LED lighting showed the highest growth rate, followed by the white, red, and blue LED lighting. Based on these results, a green light provides a suitable breeding environment for the starry flounder.

Effect of Electrode Process Variables in case of Decomposition of $NO_x$ by SPCP (연면방전에 의한 질소산화물의 분해시 전극 공정변수에 대한 영향)

  • 안형환;강현춘
    • Proceedings of the Safety Management and Science Conference
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    • 1999.11a
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    • pp.543-563
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    • 1999
  • For hazardous air pollutants(HAP) such as NO and $NO_2$ decomposition efficiency, power consumption, and applied voltage were investigated by SPCP(surface induced discharge plasma chemical processing) reactor to obtain optimum process variables and maximum decomposition efficiencies. Decomposition efficiency of HAP with various electric frequencies(5~50 kHz), flow rates(100~1,000 mL/min) initial concentrations(100~1,000 ppm), electrode materials(W, Cu, Al), electrode thickness(1, 2, 3 mm) and number of electrode windings(7, 9, 11) were measured. Experimental results showed that for the frequency of 10 kHz, the highest decomposition efficiency of 94.3% for NO and 84.7% for $NO_2$ were observed at the poser consumptions of 19.8 and 29W respectively and that decomposition efficiency decreased with increasing frequency above 20 kHz. Decomposition efficiency was increased with increasing residence times and with decreasing initial concentration of pollutants. Decomposition efficiency was increased with increasing thickness of discharge electrode and the highest decomposition efficiency was obtained for the electrode diameter of 3mm in this experiment. As the electrode material, decomposition efficiency was in order : tungsten(W), copper(Cu), aluminum(Al).

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Isolation of Nematophagous Fungi against Root-knot Nematode and Their Growth in Vitro (뿌리혹 선충에 대한 기생 천적 진균 분리 및 이들의 생장에 미치는 환경조사)

  • 정미정;김희규
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.149-158
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    • 1988
  • Nematophagous fungi were successfully isolated by baited plating, centrifugation technique of soil, and direct isolation from naturally ingested nematodes. Predominant seven fungi isolated were identified as Artheobotrys arthroboteyides, A.conoides, A. oligospora, Dactylella lobata, Fusatium oxysporum, Monacrosporium ellopsoporum and Harposporium anguillu-lae. Of these, six fungi were tested for cultural characteristics except. H, anguillulae, extre-mely fastidious fungus in artificial media. Among 14 media tested in this experiment, Corn-meal Agar (CMA) and Oatmeal Agar (OMA) were the most suitable media for growing all six nematophagous fungi. Weakly saprophytic M. ellipsospoyum also grew vigoroualy on these two media. The radial growth, dry weight and sporulation of the fungi tested were quite diverse depending on the culture media. D. lobata revealed good growth and abundantly sporulated on Glucoes Peptone Agar (GPA). Although over-all growth of F, oxysporum was not satisfactory on Sucrose Nitrate Agar (SNA), the sporulation was best on this medium. Optimum conditious for mycelial growth and sporulation of nematophagous fungi ranged pH 5-8 and 20-$30^{\circ}C$ on SNA. D. lobata and F, oxysporum grew vigorously and most profusely sporulated on all media tested. They turned out an most promising biocontrol agents for their aggressive growth and sporulation over the ranges of temperature and pH ranges.

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Development of Glucoamylase & Simultaneous Saccharification and Fermentation Process for High-yield Bioethanol (고효율 바이오 에탄올 생산을 위한 당화효소 개발 및 동시당화발효 공정 연구)

  • Choi, Gi-Wook;Han, Min-Hee;Kim, Yule
    • KSBB Journal
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    • v.23 no.6
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    • pp.499-503
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    • 2008
  • The bioethanol for use as a liquid fuel by fermentation of renewable biomass as an alternative to petroleum is important from the viewpoint of global environmental protection. Recently, many scientists have attempted to increase the productivity of bioethanol process by developing specific microorganism as well as optimizing the process conditions. In the present study, which is based on our previous investigation on the pretreatment process, theproductivity of bioethanol obtained from simultaneous saccharification and fermentation (SSF) process was compared between various domestic materials including barley, brown rice, corn and sweet potato. Additionally, Solid glucoamylase (SGA; developed in Changhae Co.), from modified strain with UV, was used. The result was compared to commercial glucoamylase (GA). It was observed that the fermentation rate was increased together with the yield which can be derived from the final ethanol concentration. Especially, in the case of brown rice, compared to the experimental results using GA, the final ethanol concentration was 1.25 times higher and 18.4 g/L of the yield was increased. Also, the time required for reaching 95% of the maximum ethanol concentration is significantly reduced, which is approximately 36 hours, compared to 88 hours using GA. It means that SGA has excellent saccharogenic power.

A Fundamental Study to Estimate Construction Performance of Subsea Waterjet Trenching Machine (해저지반 굴삭용 워터젯 장비의 시공성능 추정에 관한 기초적 연구)

  • Na, Kyoung-Won;Beak, Dong-Il;Hwang, Jae-Hyuk;Han, Sung-Hoon;Jang, Min-Suk;Kim, Jae-Heui;Jo, Hyo-Jae
    • Journal of Navigation and Port Research
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    • v.39 no.6
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    • pp.539-544
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    • 2015
  • There is drift toward moving offshore structures operating sites to deep water that brings subsea systems and types of apparatus to meet more severe environment than onshore. At this moment, climatic condition and seabed state affect trenching efficiency so trenching process is need to make steady progress in a short time. This paper is research on estimation about construction performance of waterjet trenching machine mounted on ROV trencher. Optimal number of nozzles that can maximize trenching efficiency is selected by considering clearance and angle of nozzles through CFD. Then verified effectiveness of waterjet apparatus on the result of trenching depth and velocity by model test analogized performance for construction work of waterjet trenching machine.