• Title/Summary/Keyword: 최적생육

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Optimum cultivation conditions for mass production of antagonistic bacterium Pseudomonas azotoformans HC5 effective in antagonistic of brown blotch disease caused by Pseudomonas tolaasii (버섯 세균갈색무늬병균(Pseudomonas tolaasii)에 대한 길항세균 Pseudomonas azotoformans HC5의 대량배양을 위한 최적 배양조건)

  • Lee, Chan-Jung;Moon, Ji-Won;Yoo, Young-Mi;Han, Ju-Yeon;Cheong, Jong-Chun;Kong, Won-Sik
    • Journal of Mushroom
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    • v.13 no.2
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    • pp.97-102
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    • 2015
  • This study was conducted to investigate optimum conditions for mass production of antagonistic microbes Pseudomonas azotoformans HC5. P. azotoformans HC5 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This markedly showed the antagonistic activity against P. tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the P. azotoformans HC5, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 6.0 and $15^{\circ}C$, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium was determined as follows: 0.6% adonitol, 1.5% yeast extract, 0.8% $NH_4H_2PO_4$, 5mM $MgSO_4$, and 0.2% asparagine.

Optimum cultivation conditions for mass production of antagonistic bacterium Alcaligenes sp. HC12 effective in antagonistic of browning disease caused by Pseudomonas agarici (버섯 세균성회색무늬병균(Pseudomonas agarici)에 대한 길항 세균 Alcaligenes sp. HC12의 대량배양을 위한 최적 배양조건)

  • Lee, Chan-Jung;Moon, Ji-Won;Cheong, Jong-Chun
    • Journal of Mushroom
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    • v.14 no.4
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    • pp.191-196
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    • 2016
  • This study was conducted to investigate optimum conditions for mass production of ntagonistic microbes Alcaligenes sp. HC12. Alcaligenes sp. HC12 had a potent biological control agent to control browning disease caused by Pseudomonas agarici. Alcaligenes sp. HC12 markedly showed the antagonistic activity against Pseudomonas agarici, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Alcaligenes sp. HC12, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 9.0 and $30^{\circ}$, respectively. The optimal concentration of medium elements for the growth of pathogen inhibitor bacterium(Alcaligenes sp. HC12) was determined as follows: 0.5% dextrine, 1.5% yest extract, 1.0% $NaNO_3$, 0.5% $KH_2PO_4$, and 1.5% asparagine.

Cultivation conditions for mass production of detoxifying bacterium Pseudomonas sp. HC1 of tolaasin produced by Pseudomonas tolaasii (버섯 세균성갈색무늬병원균(Pseudomonas tolaasii)의 독소(tolaasin) 저해균 Pseudomonas sp. HC1의 대량배양을 위한 최적 배양조건)

  • Lee, Chan-Jung;Yoo, Young-Mi;Han, Ju-Yeon;Jhune, Chang-Sung;Cheong, Jong-Chun;Moon, Ji-Won;Kong, Won-Sik;Suh, Jang-Sun;Han, Hye-Su;Cha, Jae-Soon
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.35-40
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    • 2014
  • Several bacteria are known as the causal agents of diseases of the cultivated button mushroom(Agaricus bisporus) and oyster mushroom(Pleurotus ostreatus). Pseudomonas tolaasii is the causal agent of brown blotch disease of commercial mushrooms. Pseudomonas sp. HC1 is a potent biological control agent to control brown blotch disease caused by Pseudomonas tolaasii. This can markedly reduce the level of extracellular toxins (i.e., tolaasins) produced by Pseudomonas tolaasii, the most destructive pathogen of cultivated mushrooms. To define the optimum conditions for the mass production of the Pseudomonas sp. HC1, we have investigated optimum culture conditions and effects of various nutrient source on the bacterial growth. The optimum initial pH and temperature were determined as pH 5.0 and $20^{\circ}C$, respectively. The optimal culture medium for the growth of tolaasin inhibitor bacterium was determined as follows: 0.9% dextrin, 1.5% yest extract, 0.5% $(NH_4)_2HPO_4$, 4mM $FeCl_3$, and 3.0% cysteine.

Biological Treatment on Wastewater of Soluble Metal Working Fluids (수용성 금속가공유 폐액의 생물학적 처리)

  • 차미선;한창민;박근태;조순자;손홍주;이상준
    • Journal of Life Science
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    • v.13 no.6
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    • pp.917-923
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    • 2003
  • The present investigation was conducted to determine the chemical oxygen demand (COD) removal efficiency by Pseudomonas aeruginosa EMS1. The COD removal efficiency in the medium containing 1% metal working fluid (MWF) was 12% after cultivation of 4 days. The composition of optimum medium for the COD removal efficiency of 1% MWF by P. aeruginosa EMS1 were NH$_4$Cl 0.3%,$ K_2HPO_4\; 0.05%,\; KH_2PO_4\; 0.04%,\; MgSO_4.7H_2O\; 0.05%,\; CaCl_2.2H_2O 0.03%$ and $FeSO_4.7H_2O$ 0.04% at initial pH 7.0 and $30^{\circ}C$. Under this condition, the highest the COD removal efficiency was observed after 4 days.

Effects of pH Level and Electrical Conductivity on Growth, Nutrient Absorption, Transpiration and $CO_2$ assimilation of Leaf Lettuce in Hydroponics (잎상추 수경재배에서 근권 pH와 EC가 무기이온흡수, 광합성, 증산량 및 생육에 미치는 영향)

  • 박미희;심미영;이용범
    • Journal of Bio-Environment Control
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    • v.8 no.2
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    • pp.115-124
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    • 1999
  • This study was conducted to determine the optimum root zone environment condition and proper nutrient management system for lettuce in hydroponics. For the root zone environment condition, several level of pH and electrical conductivity (EC) were treated respectively. Though all the level of pH 4 to pH 8, except pH 3, performed better growth without any visible physiological disorder, the optimum pH of the nutrient solution for lettuce production was pH 5.5 to 6.0. The optimum ionic strength of the solution was EC 1.2 to 1.6 mS $cm^{-1}$ / because higher nutrient level caused tip burn symptom by calcium deficiency. Considering the above results, it is concluded that lettuce can be efficiently mass-produced through the optimum root zone environment.

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A Design of Growth Measurement System Considering the Cultivation Environment of Aquaponics (아쿠아포닉스의 생육 환경을 고려한 성장 측정 시스템의 설계)

  • Hyoun-Sup, Lee;Jin-deog, Kim
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.27 no.1
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    • pp.27-33
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    • 2023
  • Demands for eco-friendly food materials are increasing rapidly because of increased interest in well-being and health care, deterioration of air quality due to fine dust, and various soil and water pollution. Aquaponics is a system that can solve various problems such as economic activities, environmental problems, and safe food provision of the elderly population. However, techniques for deriving the optimal growth environment should be preceded. In this paper, we intend to design an intelligent plant growth measurement system that considers the characteristics of existing aquaponics. In particular, we would like to propose a module configuration plan for learning data and judgment systems when providing a uniform growth environment, focusing on designing systems suitable for production sites that do not have high-performance processing resources among intelligent aquaponics production management modules. It is believed that the proposed system can effectively perform deep learning with small analysis resources.

Fruit-body Formation of Pleurotus florida on the Synthetic Medium (합성배지(合成培地)를 이용(利用)한 고온성(高溫性) 느타리버섯의 자실체(子實體) 형성(形成)에 관한 연구(硏究))

  • Hong, Jai-Sik;Kang, Kui-Hwan
    • The Korean Journal of Mycology
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    • v.11 no.3
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    • pp.121-128
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    • 1983
  • Nutritional and physio-chemical conditions for mycelial growth and fruit-body formation of Pleurotus florida were determined in synthetic media. Mannitol and sucrose were good sugar substances for the mycelial growth and fruit-body formation whereas less mycelial growth and no fruit-body formation was obtained with arabinose, lactose and inulin. The optimum concentration of mannitol was about 2%. Peptone as a nitrogen sources resulted in a rapid mycelial growth and fruit-body formation with higher yield, but nitrite-nitrogens inhibited the mycelial growth. The higher yield was obtained with 0.2% peptone. Among the vitamins used, the greatest mycelial growth and fruit-body formation brought about by thiamine and folic acid. $KH_2PO_4$ and $MgSO_4$ at 0.2% and 0.02%, respectively, were effective for the mycelial growth and fruit-body formation, but other inorganic salts used were ineffective. The optimum temperature for mycelial growth and fruit-body formation were $25^{\circ}C$ and $20^{\circ}C$, respectively, and light intensity of $100{\sim}500\;lux$ and pH 6.0 appeared to be effective.

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